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[Preliminary tests with Hemastix and Sangur test strips and Phosphatesmo KM test paper do not modify DNA typing of blood and semen stains].

Some of the commonly used presumptive test reagents for identification of blood and semen could affect the recovery or the analysis of high-molecular-weight DNA from evidentiary samples. The study presents data on restriction fragment length polymorphisms (RFLP) and polymerase chain reaction (PCR) analyses from blood and semen stains treated with Hemastix (Bayer Diagnostics), Sangur (Boehringer) and Phosphatesmo-KM-paper (Macherey-Nagel). The results demonstrate that the three tests examined do not have any effect on quality and quantity of the DNA nor on DNA typing by RFLP and PCR analyses. In conclusion, presumptive testing of blood and semen stains using Hemastix, Sangur, or Phosphatesmo paper can principally be carried out prior to submission to DNA analysis. In spite of these findings, it is recommended to continue the prudent practice of testing only small portions of an evidentiary stain in order to prevent contamination.

Blood Grouping and Crossmatching↗
PubMedDate not supplied

[A rapid penicillinase paper strip test for the detection of beta-lactamase].

The penicillinase paper strip beta-lactamase detecting test is an easy and rapid method which gives the information about some organism susceptibility to penicillins, immediately after their primary isolation. This information is important in serious infection treatment. This test is a modification of the standard iodometric test and is performed on paper penicillin and starch impregnated strips. The paper penicillinase strip test has been evaluated in speed, accuracy and value; it was compared with iodometric test, nitrocefin test and penicillin susceptibility test. The following fresh clinical isolates were used: 247 Staphylococcus aureus strains, 20 Staphylococcus epidermidis strains and 36 Haemophilus influenzae strains; total 303 isolates. The high correlation among all three tests mentioned in beta-lactamase detection was found as well as the correlation between penicillin susceptibility and this enzyme production. The sequence of susceptibility of the tests used is obtained as follows: nitrocefin test, paper penicillinase strip test, iodometric test.

Bacteria↗