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At least 559 records · Page 31Linked to original sources
PubMed1978

Rapid identification of group A, B, C and G beta-haemolytic Streptococci by a modification of the co-agglutination technique. Comparison of results obtained by co-agglutination, fluorescent antibody test, counterimmunoelectrophoresis, and precipitin technique.

A rapid modification of the co-agglutination (COA) technique for grouping A, B, C and G beta-haemolytic streptococci was developed. The results are obtained within three hours after inoculation from primary plates of 0.5 ml broth. This method was compared to the fluorescent antibody test (FA) and counterimmunoelectrophoresis (CIE), two other rapid methods available for serological grouping of streptococci. Of 71 recently isolated streptococcal strains from clinical sources, 70 were correctly grouped in COA, 63 in FA and 68 in CIE. With commercial reagents COA compared favourably in accuracy to the other methods and can be recommended for routine serological grouping of beta-haemolytic streptococci in the clinical laboratory.

Agglutination Tests↗
PubMed1958

Measurement of precipitin reactions in the millimicrogram protein-nitrogen range.

Exploitation of newer instrumentation and a dye-binding method for protein measurement made possible reduction in volume down to 1/1000 that commonly used, with no greater error. The procedure was tested at two levels, 1- and 10-microl volumes, with human gamma globulin and rabbit antiserum. Of the dyes tested, bromsulfalein proved best for the protein estimation.

Blood Proteins↗
PubMed1961

Diffusion-precipitin index to antibody avidity.

Antigen, diffusing into converging edges of an antibody depot, inhibits the precipitation unless the reaction is completed before an inhibiting antigen excess is established. Since the rate of the increase of antigen concentration depends on the size of the angle, relative avidities of similar preparations of antibody may be estimated from the angle just producing observable inhibition.

Antibodies↗
PubMed1967

Agar-gel precipitin-inhibition test for coccidioidomycosis. II. Serological test antigen studies in agar-gel.

In this study of saprophytic and parasitic growth-phase extracts of Coccidioides immitis, an antigen from the spherule culture supernatant fluid detected a specific antibody in heretofore serologically negative suspect coccidioidomycosis human sera when diffused in agar-gel. This antigen-antibody reaction occurred also in some of the serologically positive human coccidioidomycosis sera. This study indicates that this antigen-antibody reaction should be utilized as a possible routine serological test for complete serodiagnosis.

Antibodies↗