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PubMed1965

[Rheumatic tests].

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Arthritis, Rheumatoid↗
PubMed1983

[Latex agglutination reaction for the diagnosis of toxoplasmosis].

Agglutination of latex-particles sensitized with toxoplasma-antigen is a simple, fast, inexpensive test for toxoplasmosis-diagnosis. This test has been compared with passive-hemagglutination (1091 sera) and immunofluorescence (1093 sera). From the results, this new test is showed to be closer to passive-hemagglutination than indirect immunofluorescence. After studying possible false results and biological qualities it is thought that this test has good qualities to render great services for the toxoplasmosis diagnosis, and specially for epidemiological investigations.

Fluorescent Antibody Technique↗
PubMed1981

Humoral immunity in experimental thromboembolic meningoencephalitis in cattle caused by Haemophilus somnus.

Of 23 cattle inoculated IV with Haemophilus somnus, 16 (70%) died of thromboembolic meningoencephalitis. The inoculum was prepared from a minimally subcultured isolate of H somnus that was passaged through a calf by intracisternal inoculation immediately before use. Serum antibody titers, as measured by 7 serologic tests, were not correlated with the animal's susceptibility to infection. All cattle that died had a mean 4-fold increase in agglutination titer during the acute phase of the disease, 2 to 4 days after inoculation. Similarly, high acute-phase titers were demonstrated in 15 cattle with naturally occurring disease. Haemophilus somnus was isolated more frequently and in greater numbers from the CNS and urinary tract than from other organs of cattle that died.

Agglutination Tests↗
PubMed1993

[DNA probe for the detection of uropathogenic strains of P-fimbriated Escherichia coli].

BACKGROUND: P fimbria is one of the main factors of virulence of the uropathogenic Escherichia coli strains thus developing methods for its detection is of interest. P fimbriation may manifest through associated characteristic hemagglutination patterns. Another way of directly detecting its expression is by the PF test consisting in a specific agglutination test with latex particles which have the specific receptor of the fimbria incorporated. METHODS: The two phenotypic techniques (hemagglutination pattern using human, bovine, and sheep erythrocytes, and the PF test) were compared with colony hybridization with a specific DNA probe (pap1) in 35 strains of uropathogenic E. coli. RESULTS: Eight of the 35 strains studied were positive for the PF test with 7 strains presenting mannose-resistant agglutination to human erythrocytes without agglutinating the other erythrocytes tried. However, with hybridization with the DNA probe the number of positives was higher (25/35). CONCLUSIONS: The difference found in the number of positive strains may be due to the probe used corresponding to cluster pap, thus the use of a smaller more specific probe for fimbrial expression obtained from pap should be used given that the hybridization technique is easy to perform and is carried out in less time than phenotypic detection which requires long periods of culture prior to the test.

Animals↗