Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “INSEMINATION”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 559 records · Page 31Linked to original sources
PubMed1995

Sources of variation in the reproductive performance of ewes inseminated with frozen-thawed ram semen by laparoscopy.

Fertility data from 8 artificial insemination programs, involving more than 5000 ewes and 110 rams in 3 flocks, were analyzed to determine variation due to individual AI program and ram in the reproductive performance of ewes inseminated with frozen-thawed semen by laparoscopy. The semen had been previously frozen by commercial AI centers in either pellets or straws. Both AI program and individual ram affected the proportion of ewes pregnant and the number of fetuses per ewe inseminated, but not the number of fetuses per pregnant ewe. Semen samples from 97 of the rams used were analyzed on a Hamilton Thorn HTM 2000 image analyzer for sperm concentration, percentage of motile and progressively motile spermatozoa, mean progressive velocity, and mean linear index. The correlations between these traits and reproductive performance obtained after insemination were calculated. There was large variation in the quantity and quality of the frozen semen, but only the number of total and motile spermatozoa inseminated per ewe was correlated with fertility (0.25 and 0.26, respectively). Regression analysis showed that none of the traits measured were useful for predicting fertility.

Journal Article↗
PubMed1997

The role of in vitro fertilization and intracytoplasmic sperm injection in couples with unexplained infertility after failed intrauterine insemination.

OBJECTIVE: To determine an optimal insemination technique in patients undergoing IVF after failed IUI and the role of intracytoplasmic sperm injection (ICSI) in such cases. DESIGN: Prospective, randomized study in couples with unexplained infertility (n = 63) and mild endometriosis (n = 7) undergoing IVF after four IUI cycles. Sibling oocytes were randomized into standard IVF or ICSI insemination according to the order of retrieval. SETTING: In vitro fertilization program at the Instituto Valenciano de Infertilidad, Valencia, Italy. PATIENT(S): Seventy couples with unexplained infertility undergoing IVF after failing to conceive with controlled ovarian stimulation and IUI. INTERVENTION(S): In vitro fertilization and ICSI. MAIN OUTCOME MEASURE(S): Fertilization, cleavage, and embryo quality were compared in IVF- and ICSI-inseminated oocytes. RESULT(S): There was no significant difference in fertilization rates between ICSI (60.4%) and conventional IVF (54.0%). Similarly, there was no difference in embryo quality between both groups. There was no total fertilization failure in ICSI-inseminated oocytes, whereas 8 (11.4%) of 70 cases showed absence of fertilization when conventional IVF was used. CONCLUSION(S): Couples with unexplained infertility and mild endometriosis failing to conceive with IUI and undergoing IVF have an 11.4% chance of fertilization failure that can be overcome easily by using ICSI in at least some oocytes. ICSI, however, is not superior to IVF as an insemination technique in most cases. These data should be used in counseling patients.

Adult↗
PubMed1988

Successful uterine insemination of fallow deer with fresh and frozen semen.

Six fallow does were inseminated directly into the uterine horns 72 h (three does) or 78 h (three does) after the removal of progestagen intravaginal sponges. Three does were inseminated with fresh (two at 72 h and one at 78 h) or frozen-thawed (one at 72 h and two at 78 h) semen. The semen used had been collected by electroejaculation and had been stored for 2 yr in liquid nitrogen in a Tris, citric acid, glycerol diluent containing 2.25% egg yolk. Three does each produced a live fawn to insemination and all does had been inseminated 72 h after removal of sponges; two with fresh semen and one with frozen semen. The remaining three does failed to conceive to insemination, but did produce fawns to mating at a subsequent estrus.

Journal Article↗
PubMed1997

The influence of time of insemination relative to time of ovulation on farrowing frequency and litter size in sows, as investigated by ultrasonography.

The objective of this experiment was to identify the optimal time of insemination relative to the time of ovulation, based on ultrasonographic detection of embryonic survival at 10 days after ovulation, number of sows farrowing, and litter size. Furthermore, the possible value of the interval from weaning to onset of estrus for prediction of the time of ovulation was examined. Crossbred sows (n = 143) that had farrowed 2 to 9 litters were weaned (Day 0) and observed for estrus every 8 h from Day 3 until end of estrus. Ultrasonography was performed every 6 h, from 12 h after onset of estrus until ovulation had been observed. The sows were inseminated once at various time intervals from ovulation. At Day 16, 25 of the sows were slaughtered and their uteri were flushed for embryos. In the remaining sows, the number of viable and dead piglets and mummified fetuses per sow was recorded at farrowing, with the sum of the 3 constituting the total number of piglets born per sow. The highest number of embryos recovered per sow was found after insemination during the interval from 24 h before to 4 h after ovulation. The lowest frequency of non-pregnant sows and the highest total number of piglets born per sow were found after insemination from 28 h before to 4 h after ovulation. Consequently, the optimal time for insemination was found to be in the interval 28 h before to 4 h after ovulation. The interval from weaning to onset of estrus and from onset of estrus to ovulation were negatively correlated, allowing a rough prediction of the time of ovulation from the interval from weaning to onset of estrus.

Journal Article↗
PubMed1999

Effect of adding reduced glutathione during insemination on the development of porcine embryos in vitro.

This study evaluated the effect of adding reduced glutathione (GSH) during sperm washing and insemination on the subsequent fertilization dynamics and development of IVM porcine oocytes. Follicular oocytes were matured in vitro in NCSU 23 medium with porcine follicular fluid, cysteine and hormone supplements for 22 h. They were then matured in the same medium but without hormones for another 22 h. Matured oocytes were stripped of cumulus cells and co-incubated with frozen-thawed spermatozoa for 5 h. Putative embryos were cultured in NCSU 23 with BSA for either 7 h to examine fertilization parameters or 6 d to evaluate cleavage (2 d) and blastocyst rates. In Experiment 1, GSH was added to the insemination medium at 0, 0.125, 0.25 or 0.5 mM. The presence of GSH during insemination did not affect (P>0.05) rates of penetration, polyspermy, male pronuclear formation or cleavage, but did increase (P<0.05) blastocyst formation rates when added at concentrations of 0.125 (36%) and 0.25 mM (34%) compared with that of the control (0 mM; 19%). However, the numbers of inner cell mass and trophectoderm cells of blastocysts were unaffected by GSH treatment (P>0.05). The presence of GSH during insemination was found not to significantly increase intracellular glutathione concentrations of oocytes (P>0.05). In Experiment 2, addition of GSH (0.25 mM) during sperm washing did not affect cleavage or blastocyst formation rates or cell numbers (P>0.05). In conclusion, the presence of GSH during insemination improves the developmental competence of IVM pig oocytes in a dose-dependent manner.

Animals↗
PubMed1998

Effects of reducing insemination time in human in vitro fertilization and embryo development by using sibling oocytes.

PURPOSE: Recent studies showed a beneficial effect of reducing the time of sperm-oocyte interaction on fertilization, division, and implantation rates of the oocytes obtained from randomized patients. In the present study, the effects of reduced insemination time on fertilization and embryo development were evaluated by using sibling oocytes from the same patient. METHODS: A total of 464 oocytes from 36 patients was randomly allocated to be inseminated for either 1 hr (reduced) or 18 hr (regular). RESULTS: Fertilization rates were not significantly different between reduced (135/229; 59%) and regular (150/235; 64%) groups. Cleavage rates and embryo quality were similar in both groups. A total of 135 embryos (73 from the reduced and 62 from the regular group) was transferred to 36 patients. Thirty-four embryos implanted in 18 patients (25.2% implantation and 50.0% pregnancy rates). CONCLUSIONS: Fertilization, cleavage, and embryo development from 1-hr insemination is comparable, not superior, to those from an 18-hr insemination time, which is commonly used in in vitro fertilization programs. These data suggest that reduced insemination time can be used during in vitro fertilization to avoid unnecessarily longer exposure to spermatozoa.

Adult↗
PubMed2001

Traumatic insemination and sexual conflict in the bed bug Cimex lectularius.

The bed bug, Cimex lectularius, has a unique mode of copulation termed "traumatic" insemination [Carayon, J. (1966) in Monograph of the Cimicidae, ed. Usinger, R. (Entomol. Soc. Am., Philadelphia), pp. 81-167] during which the male pierces the female's abdominal wall with his external genitalia and inseminates into her body cavity [Carayon, J. (1966) in Monograph of the Cimicidae, ed. Usinger, R. (Entomol. Soc. Am., Philadelphia), pp. 81-167]. Under controlled natural conditions, traumatic insemination was frequent and temporally restricted. We show for the first time, to our knowledge, that traumatic insemination results in (i) last-male sperm precedence, (ii) suboptimal remating frequencies for the maintenance of female fertility, and (iii) reduced longevity and reproductive success in females. Experimental females did not receive indirect benefits from multiple mating. We conclude that traumatic insemination is probably a coercive male copulatory strategy that results in a sexual conflict of interests.

Animals↗
PubMed1999

Effects of oestrus synchronisation and fixed-time artificial insemination on the reproductive performance of dairy heifers.

AIM: To compare the reproductive performance of heifers after oestrus synchronisation and fixed-time artificial insemination with non-synchronised heifers bred by herd sires. METHODS: Heifers from 10 spring-calving herds were randomly divided into two groups by herd, breed and age. Heifers in one group (the synchronised group, n = 478) were synchronised with a combination of progesterone, oestradiol benzoate and PGF2alpha, and inseminated 50-54 hours after progesterone treatment. Returns to first service were resynchronised with progesterone treatment 16-21 days after the fixed-time artificial insemination. Heifers in the other group (the control group, n = 470) did not receive any treatment and were bred by herd sires. RESULTS: The conception rate of synchronised heifers to the fixed-time artificial insemination was 51.2% and to the artificial insemination after resynchronisation 40.4%. The pregnancy rate at the end of the breeding season was lower (p<0.001) for the synchronised (92.9%) than for the control (97.2%) group. The interval from start of breeding to calving was earlier for synchronised (295.9 +/- 22.5 days, mean +/- s.d.) than for control (298.5 +/- 17.3 days) heifers. CONCLUSION: Results from this study indicate that the oestrus synchronisation programme used in the present study can reduce reproductive performance by increasing the empty rate compared with natural mating.

Journal Article↗
PubMed2003

Rescue ICSI of oocytes that failed to extrude the second polar body 6 h post-insemination in conventional IVF.

BACKGROUND: Attempts to 'rescue' by ICSI oocytes that remained unfertilized 24 h after conventional IVF have generally resulted in poor outcomes. The aim of the present study was to compare the outcome of rescue ICSI performed on one group of patients 6 h after initial insemination with those of another group where rescue ICSI was performed 22 h after initial insemination. METHODS: Twenty-five patient IVF cycles provided the oocytes for rescue ICSI 6 h after initial insemination, and 20 cycles provided the oocytes for rescue ICSI 22 h after initial insemination in this retrospective study. Fertilization and cleavage rates, embryo quality, implantation, and pregnancy rates after rescue ICSI were the main outcome measures. RESULTS: A fertilization rate of 70.3% was achieved with 6 h rescue ICSI compared with 48.5% with 22 h rescue ICSI (P < 0.0001). From 6 h rescue ICSI, 12 clinical pregnancies (48.0%) resulted in three sets of twins, eight singletons and one abortion. From 22 h rescue ICSI there was one (5.0%) singleton pregnancy and delivery of a healthy baby. Likewise, the implantation rate was 20.2% from 6 h rescue ICSI compared with 1.72% from 22 h rescue ICSI (P < 0.02). CONCLUSIONS: Rescue ICSI after 6 h post-insemination (46 h post-HCG) gave better fertilization, pregnancy and implantation rates compared with rescue ICSI after 22 h when oocytes have become aged.

Adult↗
PubMed2000

Exposure of turkey sperm to a synthetic peptide before insemination increases fertility.

Effects on fertility and hatch of eggs laid by hens inseminated with sperm exposed to a synthetic peptide were studied. Pooled semen from 40 randomly selected toms was split and held in vitro for 0 or 24 h before use. Just before insemination, sperm (at 8.33x10(9) sperm/mL) were exposed for 5 min to 0.0, 0.05, 0.25, or 0.50 microM peptide. Hens (28 per group) were inseminated within less than 30 min with 250x10(6) in 30 microL. Two inseminations 24 h before onset of lay were followed by weekly inseminations through 22 or 20 wk. For sperm that was fresh or held 24 h, peptide treatment (P<0.02 or 0.01) and week of lay (P<0.01) affected fertility and hatch of total eggs set. There was no effect of peptide treatment on hatchability of fertile eggs. For fresh sperm, use of 0.25 microM peptide, but not 0.05 or 5.0 microM peptide, increased (P<0.05) fertility and hatch of total eggs set compared with the control (0 microM). Values for fertility were 94 vs. 90% and for hatch were 84 vs. 80%. Increases in hatch were especially evident for fresh sperm after approximately 13 wk of lay.

Animal Husbandry↗
PubMedDate not supplied

Exposure of thawed frozen bull sperm to a synthetic peptide before artificial insemination increases fertility.

We evaluated the effect on fertility of in vitro exposure of thawed frozen bull sperm to synthetic FertPlus peptide prior to artificial insemination (AI). The peptide represented a 60-amino acid sequence within rat prosaposin. Commercial cryopreserved semen was from three Holstein bulls. Onset of estrus in groups of Holstein nulliparous heifers was synchronized via injection of prostaglandin F2-alpha, and heifers were scheduled for AI 8-24 hours after estrus was detected. Semen was thawed, diluted to 2.4 x 10(6) sperm/ml with buffer, and split to provide control and exposed aliquots (0 or 30 microM peptide) that were incubated at 37 degrees C for 10 minutes and then were held at 32 degrees C. The two aliquots of semen then were used on an alternate basis 2-65 minutes later to inseminate females. Each AI (one per female) involved the deposit of approximately 250,000 sperm into each uterine horn. This procedure for AI was used to reduce the pregnancy rate with control semen to below the maximum value for a given bull and to facilitate detection of any beneficial effect of the peptide. For each bull, approximately 32 heifers were inseminated with control semen, and approximately 32 heifers were inseminated with peptide-exposed semen. Pregnancy was evaluated ultrasonically approximately 60 days after AI. After excluding one group of heifers with unusually low fertility, averaged across all animals, a 29% increase in pregnancy rate resulted from exposure of sperm to peptide (P < 0.04; one-tailed chi-square test; means were 48 vs. 62%). Pregnancy rates for the three bulls for control and peptide-exposed semen, respectively, were 42 and 62%, 44 and 64%, and 56 and 61%; means in the first two pairs of values tended to differ (P approximately equal to 0.10). These observations should be confirmed with sperm from other bulls used in a more conventional manner. However, with insemination of a limiting number of cryopreserved sperm, brief exposure of the thawed bull sperm to FertPlus peptide appeared to improve fertility dramatically.

Animals↗
PubMed1989

[Management of reproduction in large dairy herds under Moroccan conditions. III. Resumption of heat and anestrus after natural insemination].

This study aims at determining both the accuracy and the rate of heat detection together with the so-called post-insemination anoestrus incidence in four Moroccan dairy herds under natural or semi-hand mating insemination. 369 females (cows and heifers) were involved from 4 large dairy units in which a herd fertility survey took place on a weekly basis. Heat detection was performed several times a day by the herdsman. Blood was sampled for plasma progesterone (P) determination on the oestrus and the mating day, on day 12 later as well as on day 21 after mating. Accuracy (A) was defined as the ratio of correct oestrus on detected oestrus during the mating days. Heat detection rate (R) was defined as the ratio of oestrus detected on oestrus expected, according to the P values 21 days after mating when the females were confirmed to be cycling. Post-insemination incidence was the complementary ratio to the latter. In the Moroccan dairy unit conditions, A = 91 p. 100, and R = 49 p. 100, the post-insemination anoestrus incidence was therefore just over 50 per cent. The distribution of the non-in-heat but mated females (9 per cent) allows two roughly equal sub-populations to be distinguished: those mated in mid-luteal phase and those with a 1-2 day overlap between the follicular phase and the oestrus. Finally the high incidence of post-insemination anoestrus demonstrates the inadequacy of heat detection for pregnancy diagnosis even under natural or semi-hand mating conditions.(ABSTRACT TRUNCATED AT 250 WORDS)

Anestrus↗
PubMed1988

[Ultrastructural observations of follicular oocytes inseminated in vitro in squirrel monkeys (Saimiri sciureus)].

The ultrastructure of squirrel monkey (Saimiri sciureus) follicular oocytes inseminated in vitro was studied at the Endocrine Research Center of Michigan State University in the United States. Adult female squirrel monkeys were induced to ovulate with FSH-hCG, followed by laparoscopy 16 hours after hCG to collect ovarian follicular oocytes. A total of 45 oocytes were inseminated 21 hours after recovery by adding sperm collected by electroejaculation, followed by insemination culture for 24 hours. Inseminated oocytes were examined by light microscopy and transmission electron microscopy. Only three (6.7%) of inseminated oocytes were judged to be fertilized morphologically, but no sperm remnant was found in the cytoplasm of the oocytes. Fifteen (33.3%) had reached the second metaphase stage. As a whole, the maturation rate was 40%. Ultrastructurally, acrosome reacted sperm were observed in the cumulus cell layer, confirming the occurrence of sperm capacitation in the in vitro culture system. Cortical granules were seen throughout the cytoplasm of the germinal vesicle oocyte, especially in the vicinity of the Golgi complexes, suggesting the possibility that cortical granules originate from Golgi complexes. Metaphase chromosome, first polar body and cortical granules were observed at the second metaphase oocyte. Of three oocytes judged to be fertilized, one oocyte contained six pronucleus-like structures and another oocyte contained a pronucleus supposing fusion of two pronuclei (syngamy). But the ultrastructural morphology of a normal appearing fertilized oocyte could not be obtained. The possible cause of the low fertilization rate in the present study is supposed to be the poor follicular response and the prematurity of the cytoplasm due to repeated administrations of exogenous gonadotropins.

Acrosome↗
PubMed1988

Life table analysis of intrauterine insemination pregnancy rates.

One hundred twenty-eight couples undergoing intrauterine inseminations were retrospectively reviewed. Life table methodology was used to analyze cumulative pregnancy rates and monthly fecundability. Respective 6- and 12-month cumulative pregnancy rates for each diagnostic group receiving intrauterine insemination were: cervical factor, 28.6% and 42.8%; male factor, 16.7% and 16.7%; female immune factor, 66.7% and 100.0%; male immune factor, 37.5% and 68.8%; and empiric treatment, 60.0% and 60.0%. There was no difference in pregnancy rates between sperm processed with a swim-up in Ham's F-10 or a two-gradient Percoll system. Abnormal sperm penetration assay results in patients with male factor did significantly (p = 0.05) lower the pregnancy rate. It is concluded that if no pregnancy has occurred after six cycles of inseminations, further workup or other treatment may be initiated, but additional pregnancies can be achieved from the seventh through the twelfth cycles of intrauterine insemination.

Actuarial Analysis↗
PubMed1977

The risks and prevention of Neisseria gonorrhoeae transfer in fresh ejaculate donor insemination.

The risk of transferring gonorrhea with donor insemination was investigated in this study. Thirteen semen specimens contaminated by Neisseria gonorrhoeae were placed in containers as used in donor artificial insemination (AID) and cultured serially. Since most fresh ejaculates are used rapidly, a 2-hour peroid was used as the end-point. Ten of the thirteen ejaculates were positive on initial and 2-hour delay cultures. Three were negative by both cultures. The epidemiology of gonorrhea is reviewed, and those cases of gonorrhea reported following AID are discussed. The use of frozen semen as advocated by some is compared with the use of fresh ejaculates. It is shown that fresh ejaculates, which are more practical for many physicians and have better fertilizing capacity, can be used if proper cultures are obtained at the time of insemination. It is suggested that either frozen ejaculates with negative culture or fresh ejaculates screened by smear and cultured at the time of insemination be utilized. The use of fresh semen is possible, since results of appropriate cultures could be available and treatment instituted before clinical disease occurs.

Gonorrhea↗
PubMed1990

A prospective randomized study of pregnancy rates following intrauterine and intracervical insemination using frozen donor sperm.

Cryopreserved sperm have lowered fertility when compared with fresh sperm in artificial insemination by donor programs. The purpose of this study was to compare pregnancy rates following intrauterine insemination (IUI) and intracervical insemination (ICI) with cryopreserved sperm in a prospective trial using the patient as her own control. A total of 154 patients were randomized into alternating treatment cycles and underwent 238 cycles of IUI and 229 cycles of ICI. The pregnancy rate per treatment cycle was 9.7% following IUI and 3.9% following ICI. Treatment outcome was influenced by patient age, ovulatory status, and endometriosis. Pregnancy success correlated well with the post-thaw survival of sperm and the number of motile cells inseminated. In spite of having normal semen parameters, some donors were found to have markedly reduced sperm fecundity. We conclude that IUI with cryopreserved sperm can be an effective treatment for couples with infertility, genetic indications, or other reasons.

Adult↗
PubMed1994

Outcome of donor versus husband insemination in couples with unexplained infertility treated by in vitro fertilization and embryo transfer.

OBJECTIVE: To examine the IVF-ET outcome of couples with unexplained infertility treated by husband versus donor sperm. DESIGN: A retrospective analysis of the IVF-ET outcome of couples with unexplained infertility treated by either husband or donor sperm and in a subgroup of patients treated simultaneously by husband and donor sperm. SETTING: IVF Unit, Serlin Maternity Hospital, Tel Aviv, Israel. PATIENTS: Couples diagnosed as having unexplained infertility underwent IVF at our Unit; included were 96 couples treated by husband insemination (group A), 27 couples who received donor insemination because of azoospermia (group B), and 8 couples who sought donor insemination after having previously failed IVF (group C). RESULTS: No statistically significant difference was found between groups A and B regarding age of the females, duration of infertility, number of IVF cycles, fertilization rate, number of ETs, and pregnancy rate. Oocytes collected in group C were subdivided further into two groups: 45 were incubated with husband sperm and 46 were incubated with donor sperm. Fertilization rates were 46.6% and 50%, respectively. One pregnancy occurred. CONCLUSION: In couples with unexplained infertility who had undergone IVF-ET with husband insemination, the fertilization and pregnancy rates were similar to those of couples who were treated by donor sperm.

Adult↗
PubMed1989

Evaluation of high vaginal insemination at oocyte recovery in patients undergoing in vitro fertilization.

The value of high vaginal insemination at the time of oocyte recovery for in vitro fertilization (IVF) has been assessed. A previous study reported a dramatic increase in the incidence of pregnancy in women after high vaginal insemination (53%) compared with those without insemination (23%). Of 306 patients undergoing IVF, 97 (32%) became pregnant and 20 (21%) miscarried. Those patients with tubal damage numbered 187. Sixty (33%) became pregnant and 10 (17%) miscarried. The data were assessed according to the numbers of conceptuses replaced, the age of the patient, and whether a spontaneous luteinzing hormone surge or human chorionic gonadotropin was a trigger for ovulation, each with a significant effect on the outcome of treatment. No significant effect of the use of high vaginal insemination was found.

Abortion, Spontaneous↗