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PubMed1975

A theoretical approach of disinfectant testing.

The purpose of this study is to compare six methods of disinfectant testing, currently applied in Europe. The testing techniques are analysed into the separate elements, which can influence the activity of the disinfectant. The differences observed in these components allow to explain why in each method other parameters are evaluated and consequently, why the interpretation of the results varies from method to method. The six tests examined are (1) the suspension test of the DGHM, (2) the suspension test of the Committee on Phytopharmacy, (3) the A.O.A.C. use-dilution method, (4) the KELSEY SYKES test, (5) the surface-disinfection test of the DGHM and (6) a modified version of this latter. First of all the final concentrations of the disinfectant in the medication mixture are calculated. On the KELSEY SYKES test differs from the others, in using a much lower final dilution, up to 50% in the 3 rd stage. It is also obvious that the longer the exposure time is, the greater the killing effect on the bacteria in the medication mixture will be. To determine the germicidal activity a method needs to pass the test, the germicidal effect has been assessed starting from the inoculum applied in the medication mixture and, in case of tests that consider the absence of growth in the subcultures as end-point, the minimum extinction required. The lowest number of bacteria is applied in the inoculum of the A.O.A.C. use-dilution method (ca. 10(6)) and the highest in the modified practical surface disinfection test (ca. 10(10)). So the required germicidal effect varies from 5 (in case of the Committee on Phytopharmacy's test and the modified practical test) to more than 8 (in case of the A.O.A.C. use-dilution method). Further on, the protein content of the bacterial suspension which is added to the medication mixture, is experimentically verified. Considerable differences are observed here. In some tests the presence of organic matter is negligible (the A.O.A.C. use-dilution method, the KELSEY SYKES test under clean conditions), whereas in others (the KELSEY SYKES test under dirty conditions and the practical surface disinfection tests) the protein added equals that of serum 10-30%. Finally it is mentioned that the dilutions in the KELSEY SYKES test are made with artificial hard water. From a comparison of the factors described above it clearly appears that in the distinct tests different parameters are used and that the results of one test cannot be applied to another.

Disinfectants↗
PubMed1975

[A comparison of the results of 4 national methods for the evaluation of disinfectants in 2 laboratories (author's transl)].

At present the testing and assessment of disinfectants is carried out according to different methods in different countries. The result of this is that certain preparations or concentrations are allowed for use in some countries while they are not in neighbouring countries. A study was carried out jointly at two universities with the object of testing the reproducibility of these methods and standardizing them. Furthermore, an attempt was to be made to arrive at common test methods. In 2 labortories (A,B) we tested and compared the disinfectant activity of three disinfectant standards (phenol, aldehyde, iodophor) against Staphylococcus aureus and Pseudomonas aeruginosa (1) in the suspension test according to the "Richtlinien für die Prüfung chemischer Desinfektionsmittel" (Directions governing the testing of chemical disinfectants) of the DGHM, (2) in the suspension test of the "Dutch Commission for Phytopharmacy," (3) according to the "Use-Dilution Method" of the A.O.A.C. and (4) in the capacity test according to KELSEY and SYKES. Every individual test was repeated ten times. In addition we determined the reproducibility of the results by repeating the tests several times with one and the same culture and with different cultures (on different days). The exact method is described elsewhere (7). The statistical evaluation of the mean values (Tables 1,3,5 and 7) and variances (Tables 2,4,6 and 8) leads to the following findings: The results obtained with the various methods depend on the lab. technique and were specific to the germs and preparations tested. The reproducibility was best in the DGHM suspension test and the capacity test according to KELSEY and SYKES; the mean values of the two laboratories were closest for the Dutch test of the Commission of Phytopharmacy. The greatest mean value differences and scatter were observed with the A.O.A.C. "Use-Dilution Method." The conclusion of this investigation is that for the preliminary testing of disinfectants a method should be recommended which enables the determination of the acturl germ count reduction after varying periods of action and with well-defined organic challenge. In order to assess the disinfectant action, however, such a test - which answers most of our questions and has optimum reproducibility - must be supplemented by main tests which simulate practical conditions as near as possible.

Aldehydes↗
PubMed1975

[The meaning of the results of four national disinfectant testing techniques (author's transl)].

The bactericidal activity of three disinfectant standards has been determined by four national methods (the qualitative suspension test of the German Society for Hygiene and Microbiology DGHM, the quantitative suspension test of the Dutch Committee on Phytopharmacy, the use-dilution method of the A.O.A.C. and the Kelsey and Sykes' test) in order to compare these four testing techniques. In previous publications the results of these experiments and the degree of standardisation of the four methods have been tested. The purpose of the present study is to evaluate to what extent the results obtained by the four testing techniques do correspond. The bactericidal activity can be expressed as a germicidal effect. In theory (Tab. 1) the values of germicidal effect can be similar for the four testing techniques. In practice (Tab. 2 and 3) it has been found that with the suspension technique of the DGHM systematically higher values are obtained than in the other tests. These can not be compared to each other, because the medication times differ. Although the values of the germicidal effect obtained are widely different, it can happen that the minimum effective concentration determined by the four methods be identical as each of these methods applies different criteria. The authors have therefore calculated the minimum effective concentration which is required for the disinfectant standard by the four disinfectant testing techniques (Tab. 4). Even here, no correspondence has been found between the four methods. The lethal concentration of the disinfectant standards determined by the use-dilution method of the A.O.A.C. is thus 2 to 16 times those required by the Dutch suspension test. The difference in effective concentrations, however, is not constant and is not related to the type of disinfectant or the test organism. In consequence, there is no way of establishing a comparison between the results of the four methods with a view to setting up a conversion table. Each disinfectant testing technique has its own characteristics.

Disinfectants↗
PubMed1976

[Control of disinfection with formaldehyde].

The reliability of a simple method of formaldehyde disinfection control, based on the colour intensity of End's agar or sulphite agar, was tested in a sealed fume chamber 1.2 cubic metres in size at a constant air temperature (21 degrees C) and at a 40-per-cent or 95-per-cent humidity. The effect of formaldehyde concentration and air humidity was examined, as exerted on bactericidal effectiveness and on the colour intensity of the mentioned media. Air humidity proved to be highly important: together with formaldehyde concentration and exposure time, air humidity is the decisive factor underlying the final effect of disinfection if due temperature is maintained. The intensity of the colouring of End's agar or sulphite agar was found to depend mainly on concentration and slightly on air humidity. Hence it is recommended that this simple control should be used only for the testing of a good sealing of the disinfected space underlying the effect of the active concentration of formaldehyde for the necessary exposure time. End's agar, produced by the Imuna National Corporation, Sarisské Michal'any, gave better results. It is considered necessary for an estimation of formaldehyde disinfection effectiveness to record, at the same time, the humidity and temperature of the air in the disinfected space. In our trials at a 95-per-cent humidity level, E. coli was totally devitalized on carriers disinfected with formaldehyde developed from 25 ml formaline and St. aureus with formaldehyde developed from more than 50 ml formaline per 1 cubic metre of space. When the air humidity level was 40% the total devitalization of the mentioned bacterial strains was not achieved even with formaldehyde concentration developed from 75 ml formaline per 1 cubic metre of space.

Agar↗
PubMed1993

Comparative efficacies of soft contact lens disinfection systems against a fungal contaminant.

The efficacies of five FDA-approved soft contact lens disinfecting solutions and heat disinfection were evaluated against the mold, Beauveria bassiana. Beauveria bassiana is a ubiquitous soil fungus with a demonstrated ability to grow into a soft contact lens matrix. A stock solution of the fungus was prepared and aliquots were added to each of the following disinfection solutions: ReNu Multi-Purpose Disinfecting Solution, Opti-Free, AOSEPT Disinfection/Neutralization Solution, Lens Plus Oxysept 1, UltraCare, and a Softmate Thermal Disinfecting Unit. Hydrogen peroxide systems were neutralized immediately using the manufacturer's recommended method. After 1 min, 5 mins, 15 mins, 30 mins, 1 hour, 4 hours, and 8 hours; samples were removed, added to neutralizing broth, and streaked onto Sabouraud's Dextrose Agar plates. Our results showed that hydrogen peroxide and heat disinfection were much more effective against Beauveria bassiana than Opti-Free or ReNu.

Colony Count, Microbial↗
PubMed1995

[Mercury release from separated amalgam after the use of different disinfectants].

Set amalgam, annealed for twelve days, was cut with water cooling and sucked into eight dental units (Systematica 1062 T), equipped with an amalgam separator (Multisystem Type 1). Disinfection of the suction devices was performed with seven commonly used disinfectants according to the manufacturer's guidelines. Rinsing with water served as a control in the last experimental series. The waste water was collected at the beginning of the experiments and after disinfection. Repeating the experiments for seven times resulted in 112 specimens that were analyzed by atomic absorption spectrophotometry. The use of Green & Clean M2 and S & M matic resulted in very low releases of mercury. Disinfection with Alprojet D and Alprojet DD M2 was comparable with the control group. The release of mercury was significantly increased, if compared to Green & Clean and S & M matic (p < or = 0.05). The use of Orotol Ultra, Aseptoclean 2, and Tiutol resulted in significantly higher releases of mercury, if compared to all other disinfectants (p < or = 0.05). Disinfectants containing oxidizing agents provoke an increasing release of mercury, thus accumulating the amounts of mercury in the general environment.

Analysis of Variance↗
PubMed1996

[Experimental observation on microbicidal activity of a complex glutaraldehyde disinfectant].

The microbicidal activity of a complex glutaraldehyde disinfectant was observed. The result showed that when the temperature of the test was 20 degrees C and 10% calf serum were added to the bacterial suspensions 2% diluted solution of the disinfectant for a two-minute contact could kill 99.99% of Staphylococcus aureus, and 1% diluted solution for two minutes could kill 99.99% of E. coli. The undiluted disinfectant with five-minute contact time could destroy the antigenicity of HBsAg. The undiluted disinfectant with thirty-five minute contact and 50% diluted solution with forty-five minute contact time could completely kill the spores of Bacillus subtilis var. niger under 50 degrees C. It was discovered that the germicidal concentration of the disinfectant was four times as high as the inhibition concentration and the used concentration determinal with capacity test was four times higher than as germicidal concentration when the three concentrations were compared. This result indicated that selection of suitable inactivator was very important. The practical concentration of disinfectant should be decided under the basis of the result from capacity test.

Animals↗
PubMedDate not supplied

Color changes of denture base materials after disinfection and sterilization immersion.

This study evaluated the effects of chemical disinfectants on the color of denture base materials. Materials tested included Paladon 65 (Kulzer, Friedrichdorf, Germany), Triad VLC (Dentsply Int, York, PA), and ProBase Cold (Ivoclar, Schaan, Liechtenstein). The disinfectants were Klinex (Lever Hellas, Athens, Greece), Cidex-7 (Johnson & Johnson, East Windsor, NJ), Hibitane (Imperial Chemical, Macclesfield, UK), and Cabadol (Detax Karl Huber, Ettlingen, Germany). Denture material samples were placed in the various disinfectants and evaluated for color changes (delta E*) at intervals of 10 minutes, 10 hours, and 7 days. Changes in color were measured using a tristimulus colorimeter on 10 samples for each denture material and disinfectant combination. It was concluded that if the recommended disinfecting times are followed, no observable color changes should be anticipated for the denture materials tested. Even the long-term (7-day) immersion caused observable color changes only with Cabadol, a phenol-based disinfectant, and ProBase and Triad denture materials.

Acrylic Resins↗
PubMed1961

Evaluation of disinfection techniques for, and their effects on, rectal thermocouple catheters.

The antibacterial activities of an iodophor (Wescodyne G), a quaternary ammonium compound (Roccal), and an iodine tincture as agents for the cold disinfection of rectal catheters contaminated in vitro were determined. Following thorough cleaning with an alcoholic solution of soft soap, each of the three disinfectants tested showed satisfactory results (100% kill) in 5 min against the enteric test bacteria (Escherichia coli and Salmonella typhosa) as well as a test species of the genus Pseudomonas, among the bacteria most resistant to surface-active agents. An aqueous solution of Wescodyne G containing 75 ppm available iodine was used both as a wiping solution and for subsequent disinfection of rectal catheters contaminated in vivo. Total bacterial destruction was found to follow a 60-min soak preceded by the wiping procedure. Rectal catheters subjected to prolonged immersion in each of the test disinfectants were found to be essentially unaffected, retaining their initial calibrations within a permissible tolerance. Neither Roccal nor Wescodyne G solutions were found to measurably attack bare thermocouples. Alcoholic iodine 0.5% did, however, exert a deteriorating effect on bare thermocouples in a short time, as measured by change in resistance characteristics. The results of this study have led to the recommendation that Wescodyne G containing 75 ppm available iodine be used in standing operating procedures for the initial cleaning and subsequent disinfection of rectal thermocouple catheters.

Benzalkonium Compounds↗
PubMed2002

Development and application of a standardized assay for chemical disinfection of coccidia oocysts.

The development and application of a standardized model for testing of anticoccidial disinfectants are described. Due to its economic impact, tenacity of oocysts, and reproducibility of the course of infection Eimeria tenella has been chosen as test organism. Oocysts of the Houghton strain were more susceptible to disinfection with 4% TP4 (Preventol) than oocysts of a field isolate (FI 292/1) as determined by sporulation inhibition and lysis. Scoring of intestinal lesions and of oocyst numbers in mucosal scrapings in chicken infected with various doses of oocysts were found unsuitable for assessment of disinfectants. Because strain differences were observed only Houghton strain oocysts were applied for further testing. Guidelines for standardized in vivo testing of disinfectants have been stipulated by the German Veterinary Society (DVG) on the basis of these studies. When applied for testing of Neopredisan (NP) in two separate laboratories similar results were obtained. Inhibitory activity (IA; proportion of inactivated oocysts) of 92.9 and 90.6% were calculated for 3% NP and of 95.2 and 96.8% for 4% NP after treatment with the disinfectant over 120 min. According to the guidelines IA of at least 95% is required for certification of sufficient disinfecting efficacy by the DVG.

Animals↗
PubMed1997

The effect of disinfection on viability and function of baboon red blood cells.

Methods that have been optimized for disinfection of red blood cells before transfusion must be evaluated for their effect on red blood cell viability and function in vitro and in vivo. This study evaluates (1) in vitro effects of Panavirocide treatment and benzoporphyrin (BPD) photosensitization on baboon and human red blood cell parameters and (2) in vivo effects of five disinfectant treatments on 24 h posttransfusion survival and cell lifetimes for baboon red blood cells. The in vitro studies showed that both disinfection methods resulted in a significant reduction in red blood cell potassium, suggesting that intracellular potassium is a sensitive measure of red cell injury during disinfection. The in vivo studies demonstrated significant reductions in the 24 h posttransfusion survival of baboon red blood cells and reductions in cell lifespan treated with a Panavirocide solution, BPD photosensitization and 15 mM nonactivated sodium chlorite. No effects were seen with 250 ppm formaldehyde, aluminum phthalocyanine photosensitization or activated sodium chlorite. These in vivo data showing effects of disinfection treatments support the use of baboons in studying disinfection procedures of autologous red blood cells before attempting studies in humans.

Animals↗
PubMed1997

Inactivation of Cryptosporidium parvum oocysts and Clostridium perfringens spores by a mixed-oxidant disinfectant and by free chlorine.

Cryptosporidium parvum oocysts and Clostridium perfringens spores are very resistant to chlorine and other drinking-water disinfectants. Clostridium perfringens spores have been suggested as a surrogate indicator of disinfectant activity against Cryptosporidium parvum and other hardy pathogens in water. In this study, an alternative disinfectant system consisting of an electrochemically produced mixed-oxidant solution (MIOX; LATA Inc.) was evaluated for inactivation of both Cryptosporidium parvum oocysts and Clostridium perfringens spores. The disinfection efficacy of the mixed-oxidant solution was compared to that of free chlorine on the basis of equal weight per volume concentrations of total oxidants. Batch inactivation experiments were done on purified oocysts and spores in buffered, oxidant demand-free water at pH 7 an 25 degrees C by using a disinfectant dose of 5 mg/liter and contact times of up to 24 h. The mixed-oxidant solution was considerably more effective than free chlorine in activating both microorganisms. A 5-mg/liter dose of mixed oxidants produced a > 3-log10-unit (> 99.9%) inactivation of Cryptosporidium parvum oocysts and Clostridium perfringens spores in 4 h. Free chlorine produce no measurable inactivation of Cryptosporidium parvum oocysts by 4 or 24 h, although Clostridium perfringens spores were inactivated by 1.4 log10 units after 4 h. The on-site generation of mixed oxidants may be a practical and cost-effective system of drinking water disinfection protecting against even the most resistant pathogens, including Cryptosporidium oocysts.

Animals↗
PubMed1999

One stage full- versus partial-mouth disinfection in the treatment of chronic adult or generalized early-onset periodontitis. I. Long-term clinical observations.

BACKGROUND: A standard treatment strategy for periodontal infections often consists of 4 consecutive sessions of scaling and root planing (per quadrant, at 1- to 2-week intervals), without proper disinfection of the remaining intra-oral niches for periodontopathogens. This could theoretically lead to a reinfection of previously disinfected pockets by bacteria from an untreated region/niche. This study aimed to investigate, over an 8-month period, the clinical benefits of a one stage full-mouth disinfection in the control of severe periodontitis. METHODS: Sixteen patients with early-onset periodontitis and 24 patients with severe adult periodontitis were randomly assigned to test and control groups. The control group was scaled and root planed, per quadrant, at 2-week intervals and given standard oral hygiene instructions. A one stage full-mouth disinfection (test group) was sought by scaling and root planing the 4 quadrants within 24 hours in combination with the application of chlorhexidine to all intra-oral niches for periodontopathogens. Besides oral hygiene, the test group also rinsed twice daily with a 0.2% chlorhexidine solution and sprayed the tonsils with a 0.2% chlorhexidine spray, for 2 months. The plaque index, gingival index, probing depth, bleeding on probing, gingival recession, and clinical attachment level were recorded at baseline and at 1, 2, 4, and 8 months afterwards. RESULTS: The one stage full-mouth disinfection resulted, in comparison to the standard therapy, in a significant (P <0.001) additional probing depth reduction and gain in attachment up to 8 months. For initial pockets > or =7 mm, the "additional" probing depth reduction at the 8 month follow-up was 1.2 mm for single-rooted and 0.9 mm for multi-rooted teeth, with corresponding additional gains in attachment of 1.0 mm and 0.8 mm, respectively. The additional improvements were observed for all subgroups (adult periodontitis, generalized early-onset cases, smokers), with the largest differences in the non-smoking adult periodontitis patients. CONCLUSIONS: These findings suggest that a one stage full-mouth disinfection results in an improved clinical outcome for the treatment of chronic adult or early-onset periodontitis as compared to scaling and root planing per quadrant at 2-week intervals.

Adult↗
PubMedDate not supplied

Disinfection of wastewater using a 20-kHz ultrasound unit.

The present study explored the application of a 20-kHz ultrasound unit for the disinfection of domestic wastewater. Experiments were conducted in batch with an ultrasound probe. The disinfection efficiency of fecal coliform increased with ultrasound power input from 0.003 log kill/min at 70 W/L to 1.8 log kill/min at 1250 W/L. Disinfection data are well-described by the Chick-Watson disinfection model (coefficient of dilution, n = 2.0) using ultrasound power in place of chemical concentration. Water quality did not affect disinfection efficiency significantly. Temperature increases experienced with ultrasonic treatment were significant. Attempts to control the temperature resulted in a marked decrease in disinfection efficiency. Experiments devised to quantify the role of thermal mechanisms revealed that of the total kill produced by ultrasound approximately 52% was attributed to heat, 36% to mechanical stresses associated with ultrasonically induced cavitation, and 12% to uncharacterized synergistic effects. Successful scale-up was demonstrated using a 9.3-L pilot-scale ultrasound unit, operated under flow-through conditions.

Enterobacteriaceae↗
PubMed2001

Evaluation of in vitro antibacterial activity of some disinfectants on Escherichia coli serotypes.

Three disinfectants commonly used in poultry farms (formalin, TH4+, and Virkon-S) were chosen for the present study. The effect of disinfectant concentration and the duration of exposure to these disinfectants on the survival of Escherichia coli serotypes (O114:K-, O86, O55:K39, and O86:K60) were investigated. Formalin (0.6%), TH4+ (0.06%), and Virkon (0.5%) all killed the four serotypes within 5 min of exposure. As the disinfectant concentration decreases, the length of exposure time to kill serotype increases. At 0.03%, 0.007%, and 0.03% of formalin, TH4+ and Virkon-S concentrations failed to kill the four E. coli serotypes within 360 min, respectively. An improvement of the inhibitory effect of these disinfectants occurred when added together with the inoculum instead of an established population. The influence of formalin, TH4+, and Virkon-S on the cell morphology of E. coli O55:K39 was investigated by using transmission electron microscopy. Formalin-treated cells exhibited normal cell morphology, with the exception that the treated cell was less fimbriated, and more destruction of pili increased when formalin concentrations were doubled. Cells treated with TH4+ (0.03%) showed destruction of the cell wall and cell surface membrane after 5 min. Cell filamentation occurred at 0.015% and increased with the increase of exposure time to this drug. Spheroplasts were observed only when cells were treated with 0.125% Virkon-S for 60 min, and cell lysis started to occur when 0.25% Virkon-S was applied for 15 min. Scanning electron microscope study revealed that Virkon-S at 0.03% and TH4+ at 0.007% completely prevented the adherence of E. coli O55:K39 serotype to chicken tracheal organ, whereas formalin (0.03%) disinfection minimized the adherence of E. coli cells to tracheal explants after 360 min of incubation.

Journal Article↗
PubMed2006

Characterization of the microbial flora in disinfecting footbaths with hypochlorite.

Change or disinfection of footwear are measures to prevent cross contamination between areas with low and high hygienic levels in the food industry. The efficacy of disinfecting footwear is not well documented. Samples of used disinfectant and from swabbing of corners after draining were taken from disinfecting footbaths containing chlorine in four Norwegian cheese factories. Bacteria were present in 9 of 12 footbaths and more positive samples were found from swab samples than from used disinfectant. The microbial flora in footbaths varied between the dairies. In two dairies, the flora was dominated by Pseudomonas spp. and Acinetobacter spp., respectively. In the third dairy, both Bacillus spp. and Staphylococcus spp. were present and in the fourth dairy, the flora was diverse (Acinetobacter sp., Enterococcus faecalis, Klebsiella pneumoniae, and Bacillus sp.). The strains were not resistant to the recommended user concentration of chlorine in bactericidal suspension or surface tests. The degree of attachment to plastic varied between strains and species and bacteria attached to surfaces were in general more resistant than suspended bacteria. The results of the survey indicated that disinfecting footbaths containing chlorine may act as contamination sources in food factories and should not be used without regular hygienic monitoring.

Bacteria↗
PubMed2004

In vitro effect of cavity disinfectants on the bond strength of dentin bonding systems.

OBJECTIVE: To evaluate the effect of two cavity disinfectants, a 2% chlorhexidine and a 1% benzalkonium chloride solution, on the shear and tensile bond strengths of dentin bonding systems to dentin. METHOD AND MATERIALS: Superficial dentin was exposed from 84 freshly extracted human third molars. The teeth were randomly assigned to two main groups according to the bonding agent used, either One Step or Optibond Solo. Each dentin bonding system had six test groups (three for shear, three for tensile testing), and each test group had a control and two cavity disinfectant groups. In the control groups, dentin bonding systems were applied after etching the dentin, whereas in the cavity disinfectant groups, dentin was conditioned and treated for 20 seconds with the disinfectants before applying the dentin bonding systems. A hybrid resin composite then was applied to all treated samples. After storage in distilled water at 37 degrees C for 24 hours, shear and tensile tests were performed. Data were analyzed using Kruskall-Wallis and Mann-Whitney U tests. RESULTS: The mean shear and tensile bond strengths of One Step and Optibond Solo were not significantly different from each other, and the cavity disinfectants also had no significant effects on shear and tensile bond strength values versus the controls. CONCLUSION: The results indicate that the use of 2% chlorhexidine and 1% benzalkonium chloride solutions as cavity disinfectants after etching the dentin did not affect the shear and tensile bond strengths of One Step and Optibond Solo.

Anti-Infective Agents, Local↗
PubMed2004

Effect of cavity disinfectants on the sealing ability of nonrinsing dentin-bonding resins.

OBJECTIVES: The purpose of this study was to determine the effect of three cavity disinfectants (chlorhexidine gluconate-based, Consepsis; benzalkonium chlorite-based, Tubulicid red; iodine-potassium iodide/copper sulphate-based, Ora-5) on the microleakage of nonrinsing dentin-bonding systems, Clearfil SE Bond and Prompt L-Pop. METHOD AND MATERIALS: Class V cavity preparations were placed on the buccal and lingual surfaces of extracted molars with occlusal margins at the enamel and gingival margins in cementum. In the experimental groups, cavities were treated with combinations of one of the three cavity disinfectants with either Clearfil SE Bond or Prompt L-Pop. The preparations without disinfectant application were used as the negative controls for each adhesive system, and the cavities in which neither disinfectant nor dentin-bonding resin were applied, served as the positive controls. After the cavity preparations were restored with resin composite, specimens were thermocycled, stained, and sectioned to evaluate dye penetration. The tooth-resin composite interface of the sectioned specimens was examined under scanning electron microscopy. RESULTS: Consepsis and Tubulicid red did not significantly affect the sealing ability of Clearfil SE Bond and Prompt L-Pop. Ora-5 exhibited gap formations at the tooth-resin composite interface and produced significantly higher microleakage when used with these bonding systems. CONCLUSION: Consepsis and Tubulicid red can be used as cavity disinfectants with Clearfil SE Bond and Prompt L-Pop without affecting their sealing abilities. However, Ora-5 is not an appropriate disinfectant to use with these bonding systems.

Anti-Infective Agents, Local↗