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At least 559 records · Page 31Linked to original sources
PubMed2003

[Theoretical analysis on comprehensive national power for sustainable development].

The connotation of comprehensive national power (CNP) was studied, the relationships between CNP and other related notions were comparatively analyzed, and the process for the study of CNP was reviewed. Based on the principles of sustainable development and the idea of ecosystem services, the concept of comprehensive national power for sustainable development (CNPSD) was proposed, and the main significance of CNQSD was discussed.

China↗
PubMed2005

Identifying genes with differential expression in gemcitabine-resistant pancreatic cancer cells using comprehensive transcriptome analysis.

Pancreatic cancer is often unresectable at diagnosis, and chemotherapy using gemcitabine is now the standard treatment for advanced pancreatic cancer. However, acquired resistance to gemcitabine resulting in therapeutic failure is often encountered. Therefore, we sought to identify genes that determine gemcitabine resistance by evaluating the relationship between gene expression profiles and gemcitabine sensitivity to provide molecular targets for overcoming gemcitabine resistance. First, the gemcitabine concentration needed for 50% growth inhibition was examined in six pancreatic cancer cell lines. By exposing MIA PaCa-2 cells to long-term gemcitabine, we established gemcitabine-resistant cells. The gene expression profiles of the six pancreatic cancer cell lines and gemcitabine-resistant cells were determined using cDNA microarray analysis. By comparing the results, 30 genes were identified as differentially expressed genes correlated with gemcitabine sensitivity. Differentially expressed genes in the parental cell lines were also examined, and six overlapping genes were identified as genes correlated with gemcitabine sensitivity in both assays. Of these genes, the down-regulated expression of TNFSF6 protein, also known as Fas ligand, was confirmed in the gemcitabine-resistant cell line. These results should provide therapeutic molecular targets for overcoming gemcitabine resistance.

Antimetabolites, Antineoplastic↗
PubMed2026

[Comparative analysis of comprehensive treatment outcomes in patients with chronic bacterial prostatitis with the addition of the multicomponent complex AndrOPREN].

INTRODUCTION: Type II chronic bacterial prostatitis is characterized by persistent infection, insufficient efficacy of standard therapy, and a high recurrence rate, which necessitates the search for additional treatment options. AIM: To evaluate the efficacy and safety of adding the multicomponent complex AndrOPREN to comprehensive therapy for type II chronic bacterial prostatitis. MATERIALS AND METHODS: This prospective, comparative, randomized study included 233 patients allocated to the main group (n=126) and the control group (n=107). In both groups, patients received standard therapy; men in the main group additionally received the multi-ingredient complex AndrOPREN at a dose of two capsules of No. 1 and two capsules of No. 2 daily for 1-2 months. The follow-up period was 60 days. Changes in symptoms according to the IPSS and QoL scores, urinalysis parameters, microscopy findings of expressed prostatic secretions, pathogen eradication, and biochemical safety parameters were assessed. RESULTS: Improvement was observed in both groups and was more pronounced in the main group. By day 14, the median IPSS score was 14.0 [12.0; 16.0] vs. 18.0 [15.0; 20.0] in the control group (p<0.001); by day 60, the corresponding values were 7.0 [5.0; 9.0] and 11.0 [9.0; 13.0] (p<0.001). At the end of follow-up, the QoL score was 2.0 [1.0; 2.0] and 3.0 [2.0; 3.0], respectively. No microbial growth was detected in 91.2% and 80.4% of patients, respectively (p=0.026); Escherichia coli eradication was achieved in 91.7% and 76.9%, respectively. No biochemical changes indicative of nephrotoxicity or hepatotoxicity were detected. DISCUSSION: The addition of the multicomponent complex AndrOPREN was associated with more rapid symptom resolution, a reduction in inflammatory changes, restoration of the secretory function of the prostate, and greater microbiological efficacy. CONCLUSION: The addition of the multicomponent complex AndrOPREN to comprehensive therapy for type II chronic bacterial prostatitis improves treatment efficacy while maintaining a favorable safety profile.

Humans↗
PubMed1996

[Comprehensive neuropsychological analysis of epileptic children with WISC-R, including profile analysis of subtests].

To evaluate intellectual functions of epileptic children, the Wechsler Intelligence Scale for Children-Revised (WISC-R) was performed on 69 children with epilepsy, and the test results were compared with their clinical symptoms and electroencephalographical findings. Both verbal IQ (VIQ) and performance IQ (PIQ) were significantly lower in the symptomatic group than in the idiopathic or cryptogenic groups, and also significantly lower in patients receiving polytherapy than monotherapy. PIQ was significantly low in patients treated with carbamazepine (CBZ), in those with poor control of seizures, in clumsy children, and cases with poor performance on visuo-motor tests. In the last two categories of patients there was an increased difference between VIQ and PIQ (discrepancy), and profile analysis revealed an impairment in non-verbal cognition and visual organization. On EEG records the patients with frontopolar (Fp) or frontal (F) focus had a significantly lower PIQ and an increased discrepancy. Migration of the epileptic focus during the periodic monitoring of EEG was also an important factor for an increased discrepancy. Furthermore, profile analysis suggested the impairment of the functions of the cerebral cortex associated with epileptic foci. These results indicate that either motor disability, such as clumsiness and minor motor disturbance, or epileptic focus on EEG are important factors affecting the assessment of neuropsychological aspects in epileptic children.

Adolescent↗
PubMed1994

Remotely related sequences and structures: analysis and predictive modelling.

Through the comprehensive analysis of protein sequence and structural data, relationships can be established that suggest, with varying degrees of success, structural models for a protein for which only the sequence is known. The certainty with which a model can be proposed depends on the degree of similarity between the sequence of unknown structure and the sequence of a protein of known structure. Methods are being developed to detect remote similarities between sequences or structures, and to predict protein structure based on such small levels of similarity.

Amino Acid Sequence↗
PubMed2001

Sequence and analysis of the Arabidopsis genome.

The comprehensive analysis of the genome sequence of the plant Arabidopsis thaliana has been completed recently. The genome sequence and associated analyses provide the foundations for rapid progress in many fields of plant research, such as the exploitation of genetic variation in Arabidopsis ecotypes, the assessment of the transcriptome and proteome, and the association of genome changes at the sequence level with evolutionary processes. Nevertheless, genome sequencing and analysis are only the first steps towards a new plant biology. Much remains to be done to refine the analysis of encoded genes, to define the functions of encoded proteins systematically, and to establish new generations of databases to capture and relate diverse data sets generated in widely distributed laboratories.

Arabidopsis↗
PubMed1999

Microfabricated modules for sample handling, sample concentration and flow mixing: application to protein analysis by tandem mass spectrometry.

The comprehensive analysis of biological systems requires a combination of genomic and proteomic efforts. The large-scale application of current genomic technologies provides complete genomic DNA sequences, sequence tags for expressed genes (EST's), and quantitative profiles of expressed genes at the mRNA level. In contrast, protein analytical technology lacks the sensitivity and the sample throughput for the systematic analysis of all the proteins expressed by a tissue or cell. The sensitivity of protein analysis technology is primarily limited by the loss of analytes, due to adsorption to surfaces, and sample contamination during handling. Here we summarize our work on the development and use of microfabricated fluidic systems for the manipulation of minute amounts of peptides and delivery to an electrospray ionization tandem mass spectrometer. New data are also presented that further demonstrate the potential of these novel approaches. Specifically, we describe the use of microfabricated devices as modules to deliver femtomole amounts of protein digests to the mass spectrometer for protein identification. We also describe the use of a microfabricated module for the generation of solvent gradients at nl/min flow rates for gradient chromatography-tandem mass spectrometry. The use of microfabricated fluidic systems reduces the risk of sample contamination and sample loss due to adsorption to wetted surfaces. The ability to assemble dedicated modular systems and to operate them automatically makes the use of microfabricated systems attractive for the sensitive and large-scale analysis of proteins.

Equipment Design↗
PubMed1991

[Staff requirements for inpatient psychiatry. "Regulation of criteria and principles for personnel requirements in inpatient psychiatry" by the Federal Government in comparison with a personnel analysis generated by the clinic].

A comprehensive analysis of the organization and personal setup was conducted at the Hans-Prinzhorn Clinic in Hemer, West Germany, from July to September 1989 after appropriate preparations that had taken about 12 months. The investigation method and the results are described. Besides the results themselves, planning and execution of this systematic study are significant in shaping the future development of this clinic. Later, and independent of our analysis, the Federal German Government promulgated a special regulation governing the setup of personal engaged in psychiatry, on 18 December 1990. The results of this regulation are compared with those obtained at our clinic. The results are practically always comparable or similar. Hence, minimum staff requirements in inpatient psychiatry were found to be near-identical as assessed by methodically different and independent analysis conducted at different times.

Germany↗
PubMed2002

[Use and comprehensibility of statistical analysis in Archivos de Bronconeumología (1970-1999)].

OBJECTIVES: To describe the type of statistical analysis used most often in original research articles published in Archivos de Bronconeumología, and the evolution of statistical analysis over time in terms of complexity. To determine comprehensibility, taking bivariate analysis as the reference threshold. MATERIAL AND METHODS: All articles published in the original research section of Archivos de Bronconeumología from 1970 through 1999 were reviewed manually. For each article we recorded the category or categories of statistical analysis and its comprehensibility (with a reference threshold set at category 7). We studied the following factors: year of publication, type of analysis, comprehensibility, maximum category achieved, subject area and number of authors. RESULTS: Eight hundred sixty original articles, with a mean 5 2 authors per article were examined. The maximum category reached was a mean 4.15 4.61. The three types of analysis used most often in all articles were category 1 (descriptive only) at 49.4%, category 2 (t and z tests) at 26.4% and category 3 (bivariate tables) at 19.1%. Among the more complex analytical categories, the most often used were analysis of variance (category 8) at 9%, survival analysis (category 16) at 6.2%, and non-parametric correlations at 3.4%. Comparing results by decade, the proportion of articles with only descriptive analysis fell from 74% in the seventies to 63.9% in the eighties and to 36.1% in the nineties (90s vs. 80s, p < 0.05; 90s vs. 70s, p < 0.05; 80s vs. 70s, p < 0.05). The use of statistics from categories 3 and 6 increased (p < 0.05), and the use of the more complex categories 8, 10, 11 and 16 increased (p < 0.05). Comprehensibility decreased significantly to 68.1% in the 90s in relation to increased complexity of analysis. CONCLUSIONS: The complexity of statistical analysis in articles published in Archivos de Bronconeumología increased over time, while comprehensibility decreased.

Analysis of Variance↗
PubMed2025

CoDIAC: A comprehensive approach for interaction analysis reveals novel insights into SH2 domain function and regulation.

Protein domains are conserved structural and functional units that serve as building blocks of proteins. Through evolutionary expansion, domain families are represented by multiple members in diverse configurations with other domains, evolving new specificities for their interacting partners. Here, we develop a structure-based interface analysis to comprehensively map domain interfaces from experimental and predicted structures, including interfaces with macromolecules and intraprotein interfaces. We hypothesized that comprehensive contact mapping of domains could yield new insights into domain selectivity, conservation of domain-domain interfaces across proteins, and identify conserved post-translational modifications (PTMs), relative to interaction interfaces, allowing for the inference of specific effects due to PTMs or mutations. We applied this approach to the human SH2 domain family, a modular unit central to phosphotyrosine-mediated signaling, identifying a novel approach to understanding binding selectivity and evidence of coordinated regulation of SH2 domain binding interfaces by tyrosine and serine/threonine phosphorylation and acetylation. These findings suggest multiple signaling systems can regulate protein activity and SH2 domain interactions in a coordinated manner. We provide the extensive features of the human SH2 domain family and this modular approach as an open source Python package for COmprehensive Domain Interface Analysis of Contacts (CoDIAC).

SH2 domains↗
PubMed2002

Impact of genomics approaches on plant genetics and physiology.

Comprehensive analysis of genetic information in higher plants is under way for several plants of biological and agronomical importance. Among them, Arabidopsis thaliana, a member of Brassica family, and Oryza sativa(rice) have been chosen as model plants most suitable for genome analysis. Sequencing of the genome of A. thaliana was completed in December 2000, and rice genome sequencing is in progress. The accumulated genome sequences, together with the hundreds of thousands of ESTs from several tens of plant species, have drastically changed the strategy of plant genetics. By utilizing the information on the genome and gene structures, comprehensive approaches for genome-wide functional analysis of the genes, including transcriptome analysis using microarray systems and a comprehensive analysis of a large number of insertion mutant lines, have been widely adopted. As a consequence, a large quantity of information on both the structure and function of genes in these model plants has been accumulated. However, other plant species may have their own characteristics and advantages to study individual phenomena. Application of knowledge from the model plants to other plant species and vice versa through the common language, namely the genome information, should facilitate understanding of the genetic systems underlying a variety of biological phenomena. Introduction of this common language may not be very simple, especially in the case of complex pathways such as a process of cell-covering formation. Nevertheless, it should be emphasized that genomics approaches are the most promising way to understand these processes.

Journal Article↗