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At least 559 records · Page 31Linked to original sources
PubMed2006

Obsessive compulsive symptoms associated with quetiapine treatment in a schizophrenic patient: a case report.

PURPOSE: Atypical antipsychotics (AAPs) are used as adjunct therapy in the treatment of resistant obsessive-compulsive symptoms (OCSs). Paradoxically other reports suggest that AAPs, particularly clozapine, risperidone, and olanzapine can induce de novo emergence or exacerbation of OCSs in psychotic patients. The authors present here the first report suggesting an association between de novo appearance of OCSs and quetiapine treatment in a schizophrenic patient. CASE: The patient was a 33-year-old woman with the diagnosis of paranoid schizophrenia, who displayed OCSs for the first time during treatment with quetiapine. The symptoms reduced remarkably when fluoxetine was added to her treatment regimen while keeping the quetiapine dosage unchanged. CONCLUSION: AAP-induced OCSs merit consideration and early identification, as these drugs are now widely in use in clinical practice. This rare but disabling side effect should also be monitored in quetiapine treated schizophrenic patients.

Adult↗
PubMed1997

Centers for disease control and prevention lead guidelines: do pediatricians know them?

American Academy of Pediatrics (AAP) fellows responded to a questionnaire measuring knowledge of 1991 Centers for Disease Control and Prevention (CDC) guidelines. Generalists' scores on a knowledge scale were positively correlated with reporting CDC and AAP documents as knowledge sources, and negatively correlated with private practice, medical school as a knowledge source, and age. However, private practitioners who read AAP documents scored well.

Adult↗
PubMed2000

Patterns in trace element chemistry in the freshwater tidal reaches of the River Trent

The major, minor and trace element chemistries at two freshwater tidal sites on the River Trent are described and compared with the non-tidal river, to demonstrate the variability in chemical processes in a major chemically and hydrologically active part of the river system, which is often overlooked in freshwater hydrochemical studies. The study shows a chemical gradient along which concentrations increase downstream for suspended sediments and acid-available particulate (AAP; > 0.45 microm) trace element fractions and also the 'dissolved' (< 0.45 microm) fractions of those trace elements with low-solubility phases. The study highlights the importance of tidally induced suspended-sediment dynamics and the generation of microparticulates for trace element transport within the tidal reaches. The microparticulate and particulate fractions are associated with both anthropogenic and lithogenous sources, and the concentrations of these two fractions co-vary according to a two-component mixing model involving riverine and tidal endmembers, with the tidal endmember exhibiting a strong lithogenous component. In many cases the AAP trace element concentrations are highly linearly correlated. At the downstream tidal site, suspended sediment concentrations were particularly high and ranged from 44 to over 24,000 mg/l as a result of tidally induced sediment mobilisation. As a consequence of this, particulate metal concentrations are especially high, and here, for the first time within the east coast studies of UK rivers, particulate fractions were measurable for trace elements such as boron and molybdenum, previously reported above detection limits only in dissolved form. It is demonstrated that the microparticulate components are not simply related to suspended sediment concentrations or to the bulk composition of the AAP fraction. Rather, microparticulate generation is related to a more complex pattern, probably linked to hydrodynamic factors involving sediment resuspension and microparticulate transport mechanisms.

Journal Article↗
PubMed2000

The water quality of the River Trent: from the lower non-tidal reaches to the freshwater tidal zone

The water quality of a major river system, the River Trent, which flows into the Humber estuary and drains large centres of population and industry in central England, is examined in terms of trace element, nutrient, major-ion, suspended sediment and carbon concentrations and set within the context of the transition from non-tidal to the tidal freshwater reaches. Detailed investigation of the water quality variability in the non-tidal river Trent has revealed mainly simple patterns in dissolved chemical concentrations, controlled largely by hydrology and the mixing of baseflow and stormflow endmember chemistries. However, silicon, carbon and nitrate show much more complex behaviour, as concentrations of these chemical determinands are regulated by in-stream biological processes. Major increases in concentrations of suspended solids and acid-available particulate (AAP) trace elements are shown to be linked to tidal mobilization of sediment and the location of the 'turbidity maximum'. Reductions in the concentrations of AAP trace elements per gram of suspended sediment suggest the mobilization of a different sediment type with a lower trace element content, which may result from: (i) a secondary sediment source; or (ii) mobilization of coarser-grained sediment under the stronger tidal hydrodynamic forces. Increased correlations for AAP-trace element interrelationships downstream suggest increasing simplicity of mixing relationships.

Journal Article↗
PubMed2005

[A comparative study of the renal damage produced after the extracorporeal shock wave lithotripsy according to the lithiasis location].

INTRODUCTION: The Extracorporeal shock waves lithotripsy (ESWL) is fundamental in the treatment of lithiasis. However, there are evidences that it can produce renal damage. The objective of our study is to determine the degree of affectation of the glomerular and tubular function after ESWL, and the influence of the lithiasis location on the type of renal damage. MATERIAL AND METHODS: A prospective longitudinal study was carried out in 14 patients with normal renal function subjected to ESWL. We determined the basal level, and the levels at the 24 hours, at the 4th and the 10th day post ESWL of: microalbuminuria (MA) (that values the glomerular function), and N-acetyl glucosamide (NAG) and alanine aminopeptidase (AAP), (that value the tubular function). RESULTS: The basal levels of of MA, NAG and AAP didn't show significant differences in connection with the localization of the stones. A significant increase was observed of the three parameters only 24 hours post ESWL. No significant differences were observed between the variation of the microalbuminuria levels, AAP and NAG and the treatment in relation to the localization of the stones. CONCLUSIONS: It exists a glomerular and tubular damage after ESWL. This damage is not related with the pelvic or calicial location of the stones. In patient with previous normal renal function, the renal damage recovers at the 4th day post ESWL.

Adult↗
PubMed2001

Screening of various Swertia species extracts in primary monolayer cultures of rat hepatocytes against carbon tetrachloride- and paracetamol-induced toxicity.

Swertia chirata Buch-Ham. (Gentianaceae), one of the oldest medicinal herbs of India, is a source of the Indian ayurvedic drug 'chirata' used for the treatment of liver disorders and malarial fevers. In this study, eight species of Swertia were collected. Each of the dry whole plant was extracted into methanol, the aqueous extract of which was sequentially extracted into hexane, chloroform and butanol extracts. The extracts were screened for their anti-hepatotoxic activity against carbon tetrachloride (CCl4) and paracetamol (acetaminophen (AAP)) toxicity in primary monolayer cultures of rat hepatocytes. The primary cultures, 2.5 x 10(6) cells /3 ml medium/60 mm collagen-coated plates, were exposed to 2.5 mM CCl4 or 12 mM AAP in the presence or absence of plant extracts (100 microg/ml culture medium). Cells and medium were harvested after 22 h of treatment for the assay of cellular reduced gluthathione (GSH) content and leakage of lactate dehydrogenase as biological end-points of toxicity. Both CCl4 and AAP at the indicated concentrations reduced GSH by almost 50 and 80%, respectively, while the enzyme leakage was almost 15% above the untreated control. Hexane and methanol extracts of most of the species in general offered relatively good protection. The anti-hepatotoxic activity, nevertheless, was evident in all Swertia species against both the toxicants. However, Swertia purpurascens, Swertia chirata, Swertia paniculata and Swertia cordata exhibited better activity compared with other species investigated. In addition, influence of various extracts (10-100 microg/ml medium) was examined on cellular growth of rat Reuber hepatoma cell line H4IIEC3/G-. Except for the butanol extract of S. chirata, no other extracts exerted toxicity in terms of neutral red uptake by the cells.

Acetaminophen↗
PubMed2002

The 1.20 A resolution crystal structure of the aminopeptidase from Aeromonas proteolytica complexed with tris: a tale of buffer inhibition.

The aminopeptidase from Aeromonas proteolytica (AAP) is a bridged bimetallic enzyme that removes the N-terminal amino acid from a peptide chain. To fully understand the metal roles in the reaction pathway of AAP we have solved the 1.20 A resolution crystal structure of native AAP (PDB ID = 1LOK). The high-quality electron density maps showed a single Tris molecule chelated to the active site Zn(2+), alternate side chain conformations for some side chains, a sodium ion that mediates a crystal contact, a surface thiocyanate ion, and several potential hydrogen atoms. In addition, the high precision of the atomic positions has led to insight into the protonation states of some of the active site amino acid side chains.

Aeromonas↗
PubMed2000

Media matters for youth health.

We live in a media world and the mass media offer educational and entertainment opportunities for adults and children. However, pediatricians and parents are increasingly aware of the potential health risks that media exposure presents to children and adolescents. For many years, the American Academy of Pediatrics (AAP) has made media education a key goal for member pediatricians: Six policy statements about the impact of mass media have been published (and revised) over the past decade. In 1997, the AAP launched a 5-year, nationwide media education campaign called Media Matters. Although education about media is the cornerstone of Media Matters, pediatricians have other important roles. In offices and clinics, the AAP suggests that pediatricians take a media history from patients and discuss connections between a child's health and behavior and media use habits. Pediatricians can also provide anticipatory guidance to families about media in the home, including limiting media use, making wise media choices, encouraging co-viewing and critical thinking, and making the home a positive media environment. Many gaps in our knowledge about the effects of media images and messages on young people still exist; pediatricians call for ongoing, targeted research about media. In this way, we can all enjoy the benefits offered by media, while minimizing the risks.

Adolescent↗
PubMed1996

The political anatomy of a guideline: a collaborative effort to develop the AHCPR-sponsored practice guideline on otitis media with effusion.

BACKGROUND: In July 1991 the Agency for Health Care Policy and Research (AHCPR) invited private organizations to develop a practice guideline on otitis media. The American Academy of Pediatrics (AAP) collaborated with the American Academy of Family Physicians, the American Academy of Otolaryngology-Head and Neck Surgery, and the Department of Pediatric Otolaryngology at the University of Pittsburgh in preparing a proposal. The AAP--the prime contractor--and the consortium were awarded the contract in October 1991. The 19-member panel assessed evidence and achieved consensus on most recommendations and maintained positive relationships that helped them function as a group. DISCUSSION: The practice guideline project has influenced the AAP's own evidence-based practice parameter methodology. Many lessons were learned. For example, since any project is only as strong as its weakest link, it was important to build the proposal on organizational strengths--including access to a combined 244,000 members and other resources. The weak links were the large number of persons involved in the project, their varying time commitments, and the wide geographic distribution of those involved. Also, neither AHCPR nor any of the organizations involved in the consortium had previous government contract experience in developing practice guidelines, so that much of the process became "on-the-job training". UPDATE: Research is needed to determine the extent to which the otitis media guideline is being used. The three academies are conducting a follow-up practice pattern variation study of pediatricians, family physicians, and otolaryngologists, which may provide data on behavior change with regard to managing the condition.

Family Practice↗
PubMed1994

Contrast nephrotoxicity: predictive value of urinary enzyme markers in a rat model.

RATIONALE AND OBJECTIVES: We tested whether urinary enzymes are an accurate and useful marker of renal damage in a rat model of contrast media nephrotoxicity. METHODS: Thirty rats were pretreated with a combination of salt depletion, indomethacin, and contrast material. Alanine aminopeptidase (AAP), gamma-glutamyltranspeptidase (GGT), and N-acetyl-beta-D-glucosaminidase (NAG) were measured before and 24 hr after injection of contrast material. Enzyme concentrations were correlated with glomerular filtration rate (GFR) and histology. RESULTS: Decreasing GFR and histopathologic changes were found only in rats treated with all three variables. NAG levels increased from baseline for both diatrizoate meglumine- and ioversol-treated animals (from 83.9 +/- 48.6 to 145.5 +/- 55.4 and from 69.41 +/- 43.6 to 123.1 +/- 50.7, respectively; P < 0.05 from baseline for ioversol) and declined in other groups. GGT and AAP levels did not correlate well with structural and functional changes. CONCLUSIONS: In this model of contrast nephrotoxicity, NAG concentration appears to correlate with structural and functional changes associated with contrast media nephrotoxicity. However, the large range of baseline values makes this of dubious clinical use. AAP and GGT levels appear less helpful in detecting renal damage.

Acetylglucosaminidase↗
PubMed2004

Aeromonas proteolytica aminopeptidase: an investigation of the mode of action using a quantum mechanical/molecular mechanical approach.

The aminopeptidase of Aeromonas proteolytica (AAP) belongs to the group of metallo-hydrolases that require two divalent cations for full activity. Such binuclear metal centers are found in several aminopeptidases, raising the question whether a common mechanism, at least partly, is likely. We have used a quantum mechanical/molecular mechanical (QM/MM) approach to investigate the reaction mechanism of AAP. Among several possibilities, one reaction path was found to be clearly the most favorable. Beside the chemical transformation steps, effects of the enzyme environment and the influence of the solvent on the catalytic reaction were included in the study. The results are in good agreement with experimental studies and correspond to a high degree to our previous QM/MM calculations on the reaction mechanism of the related binuclear bovine lens leucine aminopeptidase (blLAP), which, although related to the AAP, has different Zn(2+)-coordination spheres and a different catalytic residue. The mechanisms of the two enzymes as suggested in the literature differ on the mode of coordination of the nucleophile and the identity of the general base. However, the results of this and our previous work on blLAP allow us to identify a common mechanism for the two enzymes. This mechanism is probably quite general for binuclear zinc enzymes.

Aeromonas↗
PubMed1996

A drug resistance mutation in the inhibitor binding pocket of human immunodeficiency virus type 1 reverse transcriptase impairs DNA synthesis and RNA degradation.

Selection of the IIIB strain of human immunodeficiency virus type (HIV-1) resistant to the (alkylamino)piperidine-bis(heteroaryl)piperazine (AAP-BHAP) U-104489 results in substitution of a glycine to glutamate at residue 190 (G190E) of reverse transcriptase (RT). The AAP-BHAP resistant HIV-1 displays reduced in vitro replication capacity [Olmsted, R. A., et. al. (1966) J. Virol. 70, 3698-3705]. We report here that the G190E mutation in recombinant heterodimeric HIV-1 RT, compared to the wild-type RT (G190) or a G190A control mutant, results in a 40% and 80% reduction in the polymerase and RNase H specific enzymatic activities, respectively. A primer-extension assay that allowed determination of DNA elongation by the G190E mutant RT on a heteropolymeric HIV-1 gag-based RNA template showed an overall decrease in DNA polymerization. The size distribution of products generated by G190E RT-associated RNase H digestion of RNA from [35S]poly(rA).poly(dT) was markedly distinct from that of the G190A RT and was consistent with the observed reduction in RT-associated RNase H activity of the G190E RT. When challenged with unlabeled substrates, the G190E RT was relatively nonprocessive with respect to DNA synthesis and RNA degradation. It is concluded that the deleterious effect of the G190E resistance mutation on both of these RT functions is most likely involved in the observed retarded replication capacity of the AAP-BHAP-(U-104489-) resistant HIV-1.

Base Sequence↗
PubMed2002

Absolute asymmetric photoreactions of aliphatic amino acids by circularly polarized synchrotron radiation: critically pH-dependent photobehavior.

The absolute asymmetric photoreaction (AAP) of racemic aliphatic amino acids, such as alanine (Ala) and leucine (Leu), by left- and right-handed circularly polarized light (l- and r-CPL) irradiation was investigated in aqueous solutions at various pHs, by using the Onuki-type polarizing undulator installed in an electron storage ring. The magnitude of the optical purity (op) generated and the enantiomer-enriching mechanism operative in the AAP were found to be entirely dependent on the ionic state (and thus pH) of the amino/carboxylic acid moieties. At pH 1, the op of Ala and Leu determined by circular dichroism (CD) spectral measurement gradually developed with CPL irradiation, according to Kagan's equation. In contrast, irradiation at pH 7 gave op's much smaller than the theoretical values predicted by Kagan's equation. However, it turned out that the photodecomposition at pH 7 produces the corresponding alpha-hydroxycarboxylic acids stereoselectively, the CD sign of which is just opposite to that of the remaining amino acid, thus affording the apparently small op. It is concluded that, irrespective of solution pH, the AAP of amino acid proceeds upon CPL irradiation. At pH 1, the photodecomposition of valine, Leu, and isoleucine occurs via a Norrish type II mechanism, which is also applicable to other amino acids possessing a gamma-hydrogen. In the case of amino acids lacking a gamma-hydrogen, such as glycine and Ala, the photodecomposition mechanism is a photodeamination/hydroxylation and a Norrish type I reaction. At pH 7, the main photoproducts were ammonia and alpha-hydroxycarboxylic acids that were produced via photodeamination.

Alanine↗
PubMed2003

Inhibition of diphenolase activity of tyrosinase by vitamin b(6) compounds.

The vitamin B(6) compounds pyridoxine (PN), pyridoxamine (PM), pyridoxal (PL), and pyridoxamine 5'-phosphate (PMP) inhibited the diphenolase activity of mushroom tyrosinase. PM showed the highest inhibition; the control activity was inhibited by 38% at 1.5 mM. Each PL, PN, and PMP showed about 30% inhibition at the same concentration. Lineweaver-Burk plots showed that PM and PN were mixed-type inhibitors with K(I) values of 4.3 and 5.2 mM, respectively. Because PM and PN cannot form a Schiff base with a primary amino group of the enzyme, their inhibition is not attributable to the formation of the Schiff base. Alternatively, their quenching function of reactive oxygen species (ROS) was postulated to be responsible for the inhibition. Thus, the inhibitory effect of ROS was examined. The representative singlet oxygen quenchers l-histidine, sodium azide, Trolox, and anthracene-9,10-dipropionic acid (AAP) inhibited the activity. The specific scavenger of superoxide, proxyl fluorescamine, also inhibited the activity. The scavengers of hydroxyl radical, d-mannitol and dimethyl sulfoxide, showed no inhibition. The fluorescence of AAP was decayed during the diphenolase reaction, and PM inhibited the decay. AAP was also a mixed-type inhibitor. The results showed that the vitamin B(6) compounds inhibited the diphenolase activity by quenching ROS (probably singlet oxygen) generated during some reaction step of the diphenolase reaction.

Dose-Response Relationship, Drug↗
PubMed1996

Synthesis and bioactivity of novel bis(heteroaryl)piperazine (BHAP) reverse transcriptase inhibitors: structure-activity relationships and increased metabolic stability of novel substituted pyridine analogs.

The major route of metabolism of the bis(heteroaryl)piperazine (BHAP) class of reverse transcriptase inhibitors (RTIs), atevirdine and delavirdine, is via oxidative N-dealkylation of the 3-ethyl- or 3-isopropylamino substituent on the pyridine ring. This metabolic pathway is also the predominant mode of metabolism of (alkylamino)piperidine BHAP analogs (AAP-BHAPs), compounds wherein a 4-(alkylamino)piperidine replaces the piperazine ring of the BHAPs. The novel AAP-BHAPs possess the ability to inhibit non-nucleoside reverse transcriptase inhibitor (NNRTI) resistant recombinant HIV-1 RT and NNRTI resistant variants of HIV-1. This report describes an approach to preventing this degradation which involves the replacement of the 3-ethyl- or 3-isopropylamino substituent with either a 3-tert-butylamino substituent or a 3-alkoxy substituent. The synthesis, bioactivity and metabolic stability of these analogs is described. The majority of analogs retain inhibitory activities in enzyme and cell culture assays. In general, a 3-ethoxy or 3-isopropoxy substituent on the pyridine ring, as in compounds 10, 20, or 21, resulted in enhanced stabilities. The 3-tert-butylamino substituent was somewhat beneficial in the AAP-BHAP series of analogs, but did not exert a significant effect in the BHAP series. Lastly, the nature of the indole substitution sometimes plays a significant role in metabolic stability, particularly in the BHAP series of analogs.

Anti-HIV Agents↗
PubMed2003

Enzymuria following amphotericin B application in the rat.

The effect of amphotericin B application on urinary renal tubule enzyme excretion was investigated in rats treated with amphotericin B (1.5 mg kg-1 b.i.d., i.v.) for 4 days. Application of amphotericin B induced a significant higher daily urinary enzyme activity of the renal tubular enzymes N-acetyl-beta-glucosaminidase (NAG), beta-glucuronidase (GRS), alanine-aminopeptidase (AAP) and gamma-glutamyltransferase (GGT) in comparison with controls and sodium deoxycholate treated animals as well. A significant increase in the renal excretion of NAG, GRS, AAP and GGT occurred after the first day of amphotericin B treatment and continued until the fourth day. Following treatment for 4 days with amphotericin B urine AAP activity amounted to 69 +/- 19 U g-1 creatinine, control: 39 +/- 7 U g-1 creatinine (P < 0.05). After 4 days GGT excretion increased to 803 +/- 238 U g-1 creatinine, control: 445 +/- 106 U g-1 creatinine (P < 0.05). At the fourth day NAG excretion was 80 +/- 39 U g-1 creatinine, control: 23 +/- 5 U g-1 creatinine (P < 0.05) and GRS 724 +/- 604 U g-1 creatinine (amphotericin B), control: 276 +/- 158 U g-1 creatinine (P < 0.05). Treatment with amphotericin B decreased the creatinine clearance significantly: 0.94 +/- 0.16 ml-1 min-1 vs. control 1.35 +/- 0.29 ml-1min-1 (P < 0.05). Fractional sodium and potassium excretion was not influenced by amphotericin B. The application of sodium deoxycholate had no influence on urinary renal tubular enzyme activity. The results show that amphotericin B application induces early enzymuria of renal tubule enzymes suggesting damage of proximal renal tubules.

Acetylglucosaminidase↗
PubMedDate not supplied

Hemolytic anti-hr" (e) detectable solely by an automated polybrene technique.

An example of a hemolytic anti-hr" (e) detectable solely by an AutoAnalyzer-Polybrene (AAP) system is reported. The antibody was undetectable by routine indirect antiglobulin and enzyme tests, and produced a delayed intravascular hemolytic transfusion reaction. The anti-hr" (e) was no longer AAP detectable five months after the transfusion reaction. This paper points out the potential usefulness of the AAP technique in detecting alloantibodies in cases of transfusion reactions where no antibody specificity can be demonstrated by standard manual techniques.

Antibody Specificity↗
PubMed2003

National survey beverage consumption data for children and adolescents indicate the need to encourage a shift toward more nutritive beverages.

The American Academy of Pediatrics (AAP) recently issued recommendations for 100% fruit juice consumption for children and adolescents. National survey data (1994-1996, 1998 Continuing Survey of Food Intakes by Individuals) were used to evaluate intake of 100% fruit juice for comparison with AAP recommendations. Mean daily intakes of 100% fruit juice were 0.9, 4.6, and 3.4 ounces in children aged <6 months, 6 months-6 years, and 7-18 years, respectively, which fall within AAP recommendations for these age groups. At age 5, mean intake of fruit drinks and ades exceeded that of 100% fruit juice (P=.009). Carbonated soft drink intake exceeded that of 100% fruit juice at age 5 and of milk at age 13 (P<.04). By age 13, adolescents drank more carbonated soft drinks than 100% fruit juice, milk, or fruit drinks and ades. Increased consumption of nutrient-dense beverages (100% fruit juice, milk) and water as part of a varied diet should be encouraged.

Adolescent↗