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At least 541 records · Page 30Linked to original sources
PubMed2003

Effect of media on spore yield and thermal resistance of Bacillus stearothermophilus.

The interference of eight components in the yield of sporulation and thermal resistance to moist heat (121 degrees C) of Bacillus stearothermophilus spores suspended in 0.02 M calcium acetate solution and inoculated on paper strips previously treated with calcium acetate/calcium hydroxide was studied. The spore yield of 1.0 x 10(8)mL was developed at 62 degrees C in 17 media containing different concentrations of D-glucose, sodium chloride, L-glutamic acid, yeast extract, peptone, manganese sulfate, potassium phosphate, and ammonium phosphate. The combined effects of yeast extract, peptone, and glucose contributed positively to the spore yield and to the stability of the thermal resistance of both spores in suspension and on strips.

Culture Media↗
PubMed1997

A computationally feasible test day model for genetic evaluation of yield traits in the United States.

A multitrait analysis of test day yields is proposed that includes 60 traits [3 yield traits (milk, fat, and protein), 2 parity groups (first and later) per yield trait, and 10 stages of lactation per parity]. To reduce the computations needed for the 60 traits, test day effects are estimated within the herd before analysis across herds, the rank of t he genetic (co)variance matrix is reduced, a canonical transformation is used with missing values replace by their expectations, and a repeatability model is applied to allow inclusion of parities after second. Historical 305-d records are included through their correlations with test day effects. Possible benefits from this model include 1) more accurate estimation of environmental effects from including the influence of particular days of recording, 2) optimal use of information from all test days (especially for lactations with long intervals from calving to first test or between tests), 3) improved accuracy of evaluations for component yields through contributions from information for milk yield, and 4) greater stability of bull evaluations from accounting for genetic differences among daughters in the shape of lactation curve and maturity rate.

Animals↗
PubMed1998

Treatment of humeral fractures by closed reduction and retrograde intramedullary Ender nails.

A retrospective review of 94 humeral shaft fractures stabilized with retrograde Ender nails was performed to determine the safety and efficacy of this technique. Follow-up averaged 71 weeks. Eighty-six (91.5%) healed with no additional intervention (average: 14 weeks). There were eight (8.5%) nonunions. The functional results were good in 74 (81%), fair in 12 (13%), and poor in 5 (6%). There was one radial nerve palsy secondary to the operative procedure. The reoperation rate, including the repair of nonunions, was 19%. We conclude that intramedullary Ender nail fixation is a relatively simple and quick procedure, especially for the multiply injured patient, that adequately stabilizes most humeral fractures, yielding overall good results.

Adolescent↗
PubMedDate not supplied

Recent developments in drug delivery.

Some recent innovative approaches to drug delivery have demonstrated that the administration of drugs can be more rigidly controlled with respect to the rate and amount of drug delivered to sites of action than from the conventional dosage forms. One category of controlled release referred to as programmed drug delivery primarily involves the application of polymers of defined specifications to release agents from either non-bioerodible membrane-controlled systems or bioerodible and non-bioerodible matrices. Also included here are the pro-drugs, inactive derivatives of drugs which are transformed into the active form in vivo but possess improved solubility, stability and disposition properties, yielding more efficient action and fewer side effects. Thus, exploitation of several routes of administration have resulted in products which are inserted ophtalmically, rectally or vaginally, implanted subcutaneously, taken orally or applied topically to achieve transdermal delivery of drugs to the systemic circulation. In several cases, release is designed to follow zero-order kinetics to achieve control of therapeutic plasma concentrations for prolonged time periods. Targeting of drugs by carrier is another form of controlled release technology. Normally administered intravenously, carriers such as liposomes, nanoparticles, microspheres, human cells and linear macromolecules are finding application in treating disease states with drugs which previously were unavailable to treatment.

Delayed-Action Preparations↗
PubMed1990

Quantification of physical and cyto-physiological conditions for the electrofusion of Saccharomyces cerevisiae.

Various conditions for obtaining hybrids of the auxotrophic mutants SH1509 and SH1512 of Saccharomyces cerevisiae by electrofusion were investigated. An AC field of 400 Vp/cm and a DC field of 2 square pulses (7 kV/cm; 60 microsec each) at an interval of 0.5 sec were effective. Treatment with 0.2 (SH1509) or 1.0 mg/ml (SH1512) Zymolyase for 1 or 1.5 hr was essential. As to the molarity of the osmotic stabilizer (sorbitol), the hybrid yield peaked at 0.6 M. The presence of CaCl2 (up to 0.4 mM) or 0.1 mM CaCl2 with 0.1 mM MgCl2 enhanced the yield. The temperature of the spheroplast suspension during pulsations also affected the yield, the most suitable temperature being 28 degrees C.

Buffers↗
PubMed2003

[Study on extraction technology of soyasaponins from residual of bean ware].

OBJECTIVE: To find out the optimum extraction technology of soyasaponins from residual of bean ware. METHODS: The optimum extraction conditions were investigated by the orthogonal design, and the content of soyasaponins was determined by UV-spectro-pho-tometry. RESULTS: The optimum extraction technology was A3B1C1, that is adding 7 times and 6 times amount of 70% alcohol and refluxing for two times and each time for 1.0 h. CONCLUSION: The selected technology showed higher yield of soyasaponins, good stability and high efficient.

Drugs, Chinese Herbal↗
PubMedDate not supplied

[Time-effect relationship for unstable chromosome exchange levels in Chernobyl clean-up workers].

The post-irradiation changes of dicentric and centric ring levels were studied in Chernobyl liquidators using the data of 507 individual chromosomal surveys of persons sampled at different time after their activities at Chernobyl NPP accident zone. The time-effect relationship within 0-10.5 years after exposure was displayed as exponential decline of the mean chromosome exchange frequency with average decay half-time 2.2 y. During 10.5-13 years after exposure the increasing and stabilization of chromosome exchange yield on the level 2-3-times higher than control was observed. In the first few months after irradiation the dicentric and centric ring frequency in liquidators had the clear reverse correlation with the duration of person's duties at the Chernobyl zone. The parameters of unstable chromosome exchange elimination were independent on the initially induced aberration yield, that resulted in earlier reaching the subcontrol level in persons who had more protracted duration of duties at the Chernobyl accident zone.

Adult↗
PubMed1992

Evidence that the hormone-binding domain of the mouse glucocorticoid receptor directly represses DNA binding activity in a major portion of receptors that are "misfolded" after removal of hsp90.

After dissociation of cytosolic heteromeric glucocorticoid receptor complexes by steroid, salt, and other methods, only 35-60% of the dissociated receptors can bind to DNA-cellulose. The DNA-binding and non-DNA-binding forms of the dissociated receptors have the same Mr and are phosphorylated to the same extent (Tienrungroj, W., Sanchez, E. R., Housley, P. R., Harrison, R. W., and Pratt, W. B. (1987) J. Biol. Chem. 262, 17347-17349). The basis for the different DNA-binding activities is unknown, but the DNA-binding fraction of the receptor has a more basic pI than the non-DNA-binding fraction (Smith, A. C., Elsasser, M. S., and Harmon, J. M. (1986) J. Biol. Chem. 261, 13285-13292). We have separated the non-DNA-binding state of the receptor from the DNA-binding state and then cleaved it with trypsin and chymotrypsin. We find that the 15-kDa tryptic fragment derived from the non-DNA-binding state of the dissociated receptor is fully competent in binding DNA, whereas the 42-kDa chymotryptic fragment containing both the hormone-binding and DNA-binding domains does not bind DNA. Trypsin cleavage of the molybdate-stabilized untransformed receptor also yields a 15-kDa fragment that is fully competent in binding DNA. Reducing agents do not restore DNA-binding to the non-DNA-binding fraction of the receptor and the hormone-binding domain can be separated from the DNA-binding domain on nonreducing gel electrophoresis. These results argue that the two domains are not linked by disulfide bridges, and they are consistent with the proposal that there are two least energy states of folding after dissociation of hsp90. A significant portion of the receptors is "misfolded" in such a manner that the steroid binding domain is directly preventing DNA-binding activity.

Animals↗
PubMedDate not supplied

[Anaerobic co-digestion of RSU and macro-algae in the Venice lagoon. Preliminary results].

In these last few years in the lagoon of Venice the phenomenon of eutrophization has increased. The possibility of turning to an anaerobic digestion of the biomass seems to be interesting. In this view, the following experiment describes a study on the co-digestion of mechanically selected algaebiomass and organic fraction of solid urban waste. The results relevant to both yield parameters and process stability are reported, which have been obtained by monitoring a 3 m3 pilot digestor during a running period of about 85 days, under different working conditions.

Anaerobiosis↗
PubMed1989

Electrofusion of IBRS2 cells and the study of their fusion process.

IBRS2 epithelial cells in monolayer culture fused at a very high frequency when exposed to high-voltage electric pulsing fields. Exposure to four repetitive electric pulses of about 1.7 kilovolts per centimeter with a duration of 100 microseconds caused more than 90 percent of the cells to become fused (multinucleate) when 1 millimolar magnesium was present in the pulsing medium. Magnesium and calcium ions in the pulsing medium had a very strong effect on the electrofusion of IBRS2 cells. Magnesium could increase not only the electrofusion yield but also the stability of the cells under the conditions of electrofusion. In contrast, calcium inhibited electrofusion and decreased the stability of the cells. Careful microscopic observation revealed the electrofusion of IBRS2 cells to be very complex, dynamic process undergoing many interesting changes. A possible explanation for the process and mechanism of electrofusion of IBRS2 cells was proposed in agreement with the experimental observation.

Animals↗
PubMed1988

Immobilized polyphosphate kinase: preparation, properties, and potential for use in adenosine 5'-triphosphate regeneration.

Polyphosphate kinase (ATP:polyphosphate phosphotransferase; EC 2.7.4.1), partially purified from Escherichia coli, has been immobilized on glutaraldehyde-activated aminoethyl cellulose with a 10% retention of enzymatic activity. The immobilized enzyme can carry out the synthesis of ATP from ADP, using long-chain inorganic polyphosphate as a phosphoryl donor. Chromatographic analyses of the product mixture produced from ADP and [32P]polyphosphate demonstrated that 98% of the 32P was incorporated into ATP, indicating that the immobilized polyphosphate kinase is substantially free from contaminating polyphosphate phosphohydrolase (EC 3.6.1.11), adenosine triphosphatase (EC 3.6.1.4), and adenylate kinase (EC 2.7.4.3). Immobilized polyphosphate kinase loses no activity when stored in an aqueous suspension for 2 months at 5 degrees C or for 1-2 weeks at 25 degrees C. It may be stored indefinitely as a lyophilized powder at -10 degrees C. Michaelis constants for ADP and polyphosphate were determined to be 160 and 120 microM, respectively, for the immobilized enzyme. A small-batch reactor was found to produce ATP linearly with time up to 65% conversion of polyphosphate into ATP and to attain greater than 85% conversion to ATP at equilibrium. The ease of purification and immobilization of E. coli polyphosphate kinase, its storage stability, the purity and yield of its ATP product, and the low values of the Michaelis constants for its substrates make it a highly promising enzyme for ATP regeneration.

Adenosine Triphosphate↗
PubMed1985

Intra-articular transfer of the iliotibial tract. Two to seven-year follow-up results.

A proximally based transfer of a composite structure of the iliotibial tract, its osseous insertion, the lateral retinaculum, and part of the vastus lateralis was used in 111 anterior cruciate-deficient knees that were followed for two to seven years. No other ligament procedures were performed simultaneously. The clinical results included a negative Lachman test in 81 per cent (ninety) of the knees, an anterior drawer test of 1+ or less in 84 per cent (ninety-three knees), and a negative pivot-shift sign in 94 per cent (104 knees). Subjectively, 74 per cent (eighty-two) of the knees were rated as excellent; 21 per cent (twenty-three), as good; and 5 per cent (six), as poor. A significant modification in surgical technique, consisting of a limited posterior dissection of the iliotibial band, yielded even better static stability than the original operation did. These results, sustained for as long as seven years, are consistent with the physiological aspects of this transfer. The surgical technique is reproducible and the immediate fixation of bone allows early motion of the knee and reasonable rehabilitation.

Adolescent↗
PubMed1985

Benzethonium chloride method for proteins adapted to centrifugal analysis.

A benzethonium chloride method for measuring protein concentrations in several types of body fluids has been adapted to the Cobas Bio centrifugal analyzer. The ability of this analyzer to add two reagents stepwise, measuring absorbances after each addition, is essential for proper blanking of urine specimens. The method has excellent curve stability and precision and yields results that correlate well with those of four other protein methods.

Autoanalysis↗
PubMed1979

[A new functional durable mold after surgical correction of vaginal aplasia (author's transl)].

Unsatisfactory long-term results following operative construction of a neo-vagina are often produced by contraction in the upper third of the blind vagina. These contraction tendencies can only be halted by use of durable molds. Standardized phalli are not, in our experience, well-suited for this purpose. Fabrication and fitting of an individual durable mold of tissue-neutral plastic, adapted to pelvic stability and function and yielding a good measure of success, are described.

Dilatation↗
PubMed1980

Nucleic acid-binding glycoproteins which solubilize nucleic acids in dilute acid. Re-examination of the Ustilago maydis glycoproteins.

Holloman ((1975) J. Biol. Chem. 250, 2993-3000) reported the isolation from Ustilago maydis of a glycoprotein which prevented the precipitation of nucleic acids in cold 5% trichloroacetic acid. Two glycoprotein fractions from U. maydis with this nucleic acid-solubilizing activity were isolated in our laboratory using improved purification procedures. The activity was not due to nuclease contamination. The glycoproteins are distinguished by: their ability to bind to concanavalin A-Sepharose; their differential binding to double- and single-stranded deoxyribonucleic acid, and to ribonucleic acid; their molecular weights (46,000 and 69,000); and the relative amounts present in growing versus nongrowing cells. Both fractions required sulfhydryl-reducing conditions for optimal yields, specific activity, and stability. Nucleic acid binding was cooperative, the minimum number of glycoproteins required to make a native T7 DNA molecule soluble in dilute acid being estimated at 2 and 15, respectively.

Basidiomycota↗
PubMed1998

Development and evaluation of an ideal formulation of glibenclamide by solid dispersion techniques.

An attempt was taken to develop an ideal formulation of Glibenclamide. Considering the physico-chemical nature of the drug the solid dispersion system was utilised using hydrophilic and hydrophobic carriers and formulations were made by fusion as well as solvent evaporation/co-precipitation techniques. The resulting formulations were evaluated in respect of physical nature, yield, assay content and stability. The in vitro release profiles of the formulations were compared with the marketed tablet and simple physical mixtures having same composition of drug and excipients. The effects of various carriers as well as manufacturing procedures on the in vitro release rate of drug were also studied. It is observed that a suitable blend of different batches of solid dispersions containing hydrophilic and hydrophobic carriers will give an optimum formulation with quick onset and prolonged duration of action.

Drug Stability↗
PubMed2000

Gemcitabine in soft tissue or bone sarcoma resistant to standard chemotherapy: a phase II study.

PURPOSE: To assess the efficacy of gemcitabine in patients with a variety of sarcomas that have failed to respond or escaped Adriamycin- and ifosfamide-based chemotherapy. PATIENTS AND METHODS: A group of 18 symptomatic heavily pretreated patients with sarcomas of bone or soft tissue received one induction course of gemcitabine at a dose of 1000 mg/m(2) per week for 7 consecutive weeks, followed by 1 week rest. Response to the induction course was assessed by interview and by repeated ancillary tests. If no progression was observed, maintenance by gemcitabine 1000 mg/m(2) per week for 3 weeks every 28 days was given until failure was clinically or radiologically evident. RESULTS: A total of 51 cycles of gemcitabine were given including 18 cycles of induction. A mean of 3.6 postinduction cycles were given to nine patients. The treatment was well tolerated by the patients. One partial response (leiomyosarcoma) and one minimal response (angiosarcoma) were observed, yielding a true objective response rate of 5.5%. An additional six patients achieved stabilization of disease (chondrosarcoma and osteosarcoma), yielding an overall progression-free rate of 44%. The median time to progression was more than 27 weeks. Clinical benefit response was observed only in those who also achieved a progression-free state. CONCLUSION: Gemcitabine was found to be effective in achieving stabilization and even a minimal response of soft tissue or bone sarcoma refractory to standard chemotherapy.

Adolescent↗
PubMed2006

Weight-change as a prognostic marker in 12 550 patients following acute myocardial infarction or with stable coronary artery disease.

AIMS: To examine the prognostic importance of weight-change in patients with coronary artery disease (CAD), especially following acute myocardial infarction (AMI). METHODS AND RESULTS: In 4360 AMI patients (OPTIMAAL trial) without baseline oedema, we assessed 3-month weight-change, baseline body mass index (BMI), demographics, patient history, medication, physical examination, and biochemical analyses. Weight-change was defined as change >+/-0.1 kg/baseline BMI-unit. Patients were accordingly categorized into three groups; weight-loss, weight-stability, and weight-gain. Our findings were validated in 4012 AMI patients (CONSENSUS II trial) and 4178 stable CAD patients (79% with prior AMI, 4S trial). Median follow-up was 2.7 years, 3 months, and 4.4 years, respectively. In OPTIMAAL, 3-month weight-loss (vs. weight-stability) independently predicted increased all-cause death [n=471; hazard ratio (HR) 1.26; 95% CI 1.01-1.56; P=0.039] and cardiac death (n=299, HR 1.33, 95% CI 1.02-1.73, P=0.034). Weight-gain yielded risk similar to weight-stability (HR 1.07, P=0.592 and 0.97, P=0.866, respectively). In CONSENSUS II, 3-month weight-loss independently predicted increased mortality (HR 3.87, P=0.008). Weight-gain yielded risk similar to weight-stability (HR 1.11, P=0.860). In 4S, 1-year weight-loss independently predicted increased mortality (HR 1.44, P=0.004). Weight-gain conferred risk similar to weight-stability (HR 1.05, P=0.735). CONCLUSION: In patients following AMI or with stable CAD, weight-loss but not weight-gain was independently associated with increased mortality risk.

Aged↗