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At least 541 records · Page 30Linked to original sources
PubMed1998

[Study on the adhesion of an endemic strain of Streptococcus mutans serotype C to acquired pellicle. I. A comparison of the methods to extract adhesins from Streptococcus mutans].

The adhesins of S. mutans serotype c endemic strain WD9463A were extracted from culture superant by cold ethol precipitation, or were extracted from cell surface by 0.5 mol/L phosphate buffer, 6 mol/L urea or 2% SDS solution. The composition and adhesive activities of the extracts were compared by SDS-PAGE and bacterial adhesion inhibition experiments. The results showed that the protein composition of the four extracts were similar, and all extracts inhibited the adhesion of the endemic strain dramatically. It indicates that four methods can be used to extract adhesins of S. mutans, but extracting adhesins from culture superant is better than the others.

Adhesins, Bacterial↗
PubMed1998

[Study on the adhesion of an endemic strain of Streptococcus mutans serotype C to acquired pellicle. II. Isolation and extraction of adhesins of an endemic strain of Streptococcus mutans serotype C].

Surface proteins of S. mutans WD9463 A(c) were separated by DEAE-Sephadex A25 Chromatography and Sepharose C1-6B Chromatography. The adhesins were distinguished by bacterial adhesin inhibition experiment and were identified by PAGE, SDS-PAGE, IEP-PAGE, immunol diffusion test, GTF activity test and annalization of the sorts of amino acid. The results showed P1, two proteins with molecular weight of 117 kD and 127 kD and two proteins with GTF activity could inhibit the adhesion effectively. On the other hand, another kind of GTF could improve the adhesion of the strain. These indicated that S. mutans may be many kinds of adhesins.

Adhesins, Bacterial↗
PubMedDate not supplied

Susceptibility of Zimbabwean Streptococcus agalactiae (group B Streptococcus; GBS) isolates to four different antibiotics.

OBJECTIVE: To establish the susceptibility of Zimbabwean GBS strains isolated from hospitalised patients to four antibiotics. DESIGN: Cross sectional survey. SETTINGS: Four regions of Zimbabwe (Bindura, Bulawayo, Harare, and Masvingo). SUBJECTS: 113 GBS isolates from hospitalised patients in Bindura, Bulawayo, Harare and Masvingo, of whom most were suffering from infectious diseases. MAIN OUTCOME MEASURES: All isolates were tested for their susceptibility to clindamycin, erythromycin, penicillin and tetracycline. RESULTS: All isolates were 100% sensitive to clindamycin, 98% to penicillin, 86% to erythromycin; 2% of the isolates showed intermediate susceptibility to penicillin and 100% showed resistance to tetracycline. CONCLUSION: Penicillin is still the antibiotic of choice for treatment of GBS infections and for intrapartum chemoprophylaxis in Zimbabwe. For patients who are allergic to penicillin, clindamycin will be the drug of choice for both treatment and/or chemoprophylactic use in Zimbabwe.

Anti-Bacterial Agents↗
PubMed1976

[The immunobiology of M-proteins of Streptococcus pyogenes. I. Isolation and purification of M-proteins from group A streptococci (Streptococcus pyogenes) type 1].

Streptococci of group A, type 1, are lysed by use of phage associated lysin, which is characterized as a N-Acetyl-D-glucosaminidase. This enzyme has no proteolytic activity. Type specific protein antigens are isolated and purified by ion exchange chromatography on CM-cellulose and DEAE-cellulose of the crude extract, concentrated by precipitation with ammonium sulfate. The immunization tests are carried out on monkeys and rabbits.

Animals↗
PubMed1991

[Epidemiology of aminoglycoside resistance in Streptococcus except in group D Streptococcus and Enterococcus].

During a ten month period (march-december 1989), all streptococcal strains isolated from clinical specimens sent to our laboratory were routinely tested for high-level resistance to aminoglycosides. High-level resistance was detected in 16 out of 475 streptococci: streptomycin (two strains), kanamycin (four strains), streptomycin and kanamycin (ten strains).

Drug Resistance, Microbial↗