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Pharyngeal mucosal pressures, airway sealing pressures, and fiberoptic position with the intubating versus the standard laryngeal mask airway.

BACKGROUND: The tube of the intubating laryngeal mask (ILM) is more rigid than the standard laryngeal mask airway (LMA), and the authors have tested the hypothesis that pharyngeal mucosal pressures, airway sealing pressures, and fiberoptic position are different when the two devices are compared. METHODS: Twenty anesthetized, paralyzed adults were randomly allocated to receive either the LMA or ILM for airway management. Microchip sensors were attached to the size 5 LMA or ILM at locations corresponding to the pyriform fossa, hypopharynx, base of tongue, posterior pharynx, and distal and proximal oropharynx. Mucosal pressures, airway sealing pressures, and fiberoptic positioning were recorded during inflation of the cuff from 0 to 40 ml in 10-ml increments. RESULTS: Airway sealing pressures were higher for the ILM (30 vs. 23 cm H2O), but epiglottic downfolding was more common (56% vs. 26%). Pharyngeal mucosal pressures were much higher for the ILM at five of six locations. Mean mucosal pressures in the distal oropharynx for the ILM were always greater than 157 cm H2O, regardless of cuff volume. There was no correlation between mucosal pressures and airway sealing pressures at any location for the LMA, but there was a correlation at three of six locations for the ILM. CONCLUSIONS: The ILM provides a more effective seal than the LMA, but pharyngeal mucosal pressures are higher and always exceed capillary perfusion pressure. The ILM is unsuitable for use as a routine airway and should be removed after its use as an airway intubator.

Adult↗

Heat generation and hemolysis at the shaft seal in centrifugal blood pumps.

The heat and hemolysis around a shaft seal were investigated. Materials were original pumps (Nikkiso HMS-15:N-original, and 3M Delphin:D-original), vane-removed pumps (Nvane(-), Dvane(-)), and a small chamber with a shaft coiled by nichrome wire (mock pump). The original pumps were driven at 500 mmHg and 5 L/min, and vane-removed pumps were driven at the same rotation number. An electrical powers of 0, 0.5, 2, and 10 W was supplied to the mock pumps. In vitro hemolytic testing showed that hemolytic indices were 0.027 g/100 L in N-original, 0.013 in Nvane(-), 0.061 in D-original, and 0.012 in Dvane(-). Measurement of heat with a thermally insulated water chamber showed total heat within the pump of 8.62 and 10.85 W, and heat at the shaft seal of 0.87 and 0.62 W in the Nikkiso and Delphin pumps, respectively. Hemolysis and heat generation of mock pumps remained low. The results indicate that the heat generated around the shaft seal was minimal. Hemolysis at the shaft-seal was considerable but not major. Local heat did not affect hemolysis. It was concluded that the shaft-seal affected hemolysis, not by local heat but friction itself.

Animals↗

The unpredictability of seal after post space preparation: a fluid transport study.

A root canal filling remaining after post space preparation is commonly expected to provide adequate seal. Coronal leakage of 30 endodontically treated teeth was measured before post space preparation using a fluid transport assay. In 10 of these teeth post space was prepared, using a two-step procedure, first to a remaining filling of 6 mm and then to 3 mm, with the leakage studied after each step. In 10 teeth the removal was done in one step to a remaining length of 3 mm. The other 10 teeth, with intact root canal fillings, served as controls and were tested twice for leakage. A significant difference was found between the sealing ability of intact fillings and that of partially removed ones (p < 0.05). The difference between the sealing ability of 3 and 6 mm remaining length group was not statistically significant. The lack of statistical differences between the 6 mm and 3 mm fillings was due to a great variability which existed among the 3 mm remaining fillings. These results suggest that 3 to 6 mm fillings provided a seal inferior to that of intact root canal fillings. Reduction of the fillings to 3 mm resulted in an unpredictable seal.

Analysis of Variance↗

Coronal microleakage of five materials used to create an intracoronal seal in endodontically treated teeth.

The purpose of this study was to quantitatively compare the sealing effectiveness of five restorative materials that were used to create an intracoronal double seal. Fifty-two extracted mandibular molars were randomly divided into five groups of 10 teeth, and one positive and one negative control tooth. The crowns were removed and the pulpal floor and canal orifices were sealed with 3 mm of one of the following materials: Amalgabond, C&B Metabond, One-Step Dentin Adhesive with AEliteflo composite, One-Step with Palfique composite, or intermediate restorative material (IRM). Each tooth was affixed to a fluid filtration device and the seal was evaluated at 0, 1, 7, 30, and 90 days. The results showed a significant (p = 0.0001) difference in leakage between the materials. At 7 days, IRM, AEliteflo, and Palfique leaked significantly more than Amalgabond or C&B Metabond. Amalgabond consistently produced the best seal of all the materials throughout the duration of the study.

Barium Compounds↗

An in vitro comparison of adhesive systems to seal pulp chamber walls.

AIM: To compare in vitro the sealing properties of five different dentine adhesive materials (Prime&Bond NT (PBNT); Prompt L-Pop (PLP); Clearfil SE Bond (CSEB); Scotchbond Multi Purpose Plus (SMPP); EBS-Multi (EBSM)) inside the pulp chamber. METHODOLOGY: Seventy-five recently extracted human molar teeth were used. The roof of the pulp chambers and roots were removed under water cooling. Pulp tissue was removed, and the canal orifices were sealed. The pulp chambers were then treated with 5% sodium hypochlorite (NaOCl) for 1 min. The teeth were randomly divided into five groups of 15 teeth each. Adhesive systems were applied to the pulp chamber walls according to the manufacturers' instructions. The samples were connected to Plexiglass plates, and a fluid filtration method was used for quantitative evaluation of leakage. Measurements of fluid movement were made at 2-min intervals for 8 min. The quality of seal of each specimen was measured immediately, after 24 h, 1 week and 1 month. The data were statistically analysed by repeated-measurements multivariate anova, Friedman test, Wilcoxon signed rank test, Kruskal-Wallis of one-way anova and Mann-Whitney U-tests. The pulp chamber wall with and without NaOCl and resin-dentine interfaces of specimens were observed under a scanning electron microscope (SEM). RESULTS: The leakage values of the materials were significantly different at different measurement periods. In all groups, leakage values decreased with time (P < 0.05). PBNT and PLP had the least leakage during immediate measurements (P < 0.05). After 1 month, leakage of all adhesive systems was not significantly different (P < 0.05). SEM observation of pulp chamber walls demonstrated that the irregular dentine surface without smear layer was present in the nontreated group. However, NaOCl application removed the collagen fibrils leaving the dentine surface smooth. At resin-dentine interfaces of specimens, no hybridization zone was observed. CONCLUSIONS: None of the materials had created a perfect seal to the pulp chamber walls. PBNT and PLP had better sealing over the short term, but over the long term, there were no differences between the materials.

Alkanes↗

Light-cured glass ionomer cement as a retrograde root seal.

A light-cured ionomer cement was investigated as a retrograde root seal, without a retrograde cavity. This was compared with the material used in a retrograde cavity, and with a conventional glass ionomer cement, as a seal. The adaptation and sealing ability of the test materials were assessed using a confocal optical microscope with a fluorescent dye. The root canals of 40 extracted human single-rooted teeth were prepared and filled with gutta-percha and sealer. All the teeth were subsequently apicected, then divided into four equal groups. In one group, a retrograde cavity was prepared, and the light-cured glass ionomer cement was placed as a retrograde root filling. No retrograde cavities were prepared in the three remaining groups. The light-cured glass ionomer cement was applied directly onto the apicected root face. Two different thicknesses of light-cured glass ionomer cement were tested, a thin layer (approximately 1 mm) in one group, and a thicker layer (> 1 mm) in another group. A conventional glass ionomer cement was used in the last group, and applied directly onto the root face in a single thickness (approximately 1 mm). In the group where the light-cured glass ionomer cement was used in a retrograde cavity, the material was often well adapted to one cavity wall, but gaps were found on the opposite wall. The light-cured and conventional glass ionomer cement retrograde root seals were well adapted to the root face, regardless of the thickness of material used. The thinly applied (approximately 1 mm) light-cured glass ionomer cement retrograde root seals permitted the least leakage.(ABSTRACT TRUNCATED AT 250 WORDS)

Analysis of Variance↗

Integrity, maintenance and caries susceptibility of sealed surfaces in adolescents receiving regular care from general dental practitioners in Scotland.

Pit and fissure sealants (sealants) are recognized as an effective caries preventive measure. The aim of this study was to assess the fate of occlusal surface sealants in a sample of 'regularly' attending adolescent patients (mean age 12.5 years at baseline) of 41 general dental practitioners, participating in a 3-year prospective clinical trial. A subsidiary aim was to compare the caries status of completely sealed surfaces with that of inadequately sealed surfaces over the same period. Baseline and three annual follow-up examinations were conducted by one trained and calibrated examiner under optimal conditions (dental chair, operating light, and compressed air). Sealed occlusal surfaces were evaluated for sealant type, sealant coverage, and caries status of the surface. Data for the initial and final year examinations were available for 402 subjects, who had 1377 sealants at baseline. Surfaces with optimal sealant coverage at baseline (n = 592) were significantly less likely to have become decayed (dentinal caries) or to have been restored by year 4 than surfaces with less than complete coverage (P < 0.001). Considering sealant maintenance during the study, 484 of the sealants were of optimal coverage (covered the fissure system) at the final examination. However, only 135 surfaces demonstrated evidence of maintenance by attaining optimal coverage. It is concluded that the fissure sealants in this sample may not have received the regular maintenance required to ensure full surface coverage and thus provide maximal caries protection, as inadequately sealed surfaces were more likely to decay than completely sealed surfaces.

Adolescent↗

Frequency of seal disruption with the sarns centrifugal pump in postcardiotomy circulatory assist.

We have used the Sarns centrifugal pump for uni- or biventricular assist in 58 patients with postcardiotomy cardiogenic shock. This device utilizes a spinning impeller pump that is magnetically coupled to a motor imparting rotary motion to incoming perfusate. Nine patients (16%) experienced 22 device failures, which consisted of a nonvisible disruption of the seal within the pumphead. This allowed fluid to accumulate between the pumphead and the motor necessitating change of the pumphead. The time to seal disruption was 10-149 h (median 48). Of the 22 seal disruptions, 18 occurred in 73 left ventricular pumps (25%), and 4 occurred in 38 right ventricular pumps (11%) p = 0.015. Left ventricular pumps failed at 10-144 h (median 48), and right ventricular pumps failed at 48-149 h (median 83) p = 0.02. The Sarns centrifugal pump is dependable for its intended use of cardiopulmonary perfusion. However, when used for postcardiotomy assist, seal disruption should be expected. It occurs sooner and is more common during left ventricular assist. We recommend inspection of the magnet chamber for evidence of seal disruption every 12 h with left ventricular assist and every 24 h with right ventricular assist.

Adolescent↗

Bursting tension of medium and large canine arteries sealed with ultrasonic energy or suture ligation.

OBJECTIVE: To compare bursting tension of arteries > or =3 mm in diameter sealed with ultrasonic energy (UE) with arteries ligated with suture. STUDY DESIGN: Experimental, nested factorial design. SAMPLE POPULATION: Vascular segments from canine cadavers. METHODS: Arterial segments (12) were collected from each of 16 canine cadavers and equally divided into 2 groups based on vessel diameter (medium, 3.0- < 4.5 mm; large, 4.5 - < or =6.0 mm). Arterial specimens (3) from each group were sealed with either UE (Harmonic Scalpel, HS, power level 3) or suture ligation. The mean bursting pressure and tension were determined and compared. RESULTS: Bursting tension was significantly higher (P< .0001) for sutured arteries than UE-occluded arteries, irrespective of size. Bursting tension was significantly higher (P=.0013) for medium than large UE-occluded arteries, whereas there was no difference associated with size for ligated arteries. UE energy failed to seal 1 medium artery and 7 large arteries. Compared with normal blood pressure, bursting pressures were 3.5 times greater for sutured arteries irrespective of size, 1.8 times greater for medium UE-occluded arteries, and approached normal blood pressure for large UE-occluded arteries. CONCLUSION: At the power level tested, UE should not be used to seal arteries > 4.5 mm in diameter. Suture provided an optimal seal for arteries < or =6.0 mm in diameter. CLINICAL RELEVANCE: Use of UE to occlude arteries during laparoscopic surgery is advantageous because only 1 instrument is required to simultaneously cut and coagulate tissue, but care should be exercised where large arteries might be encountered.

Animals↗

Testing of sealing ability of endodontic filling materials.

Incomplete obturation of the root canal system is the major cause of endodontic failure. Endodontic filling materials with ability to seal the root canal are, therefore, essential for successful endodontic therapy. However, assessment of sealing ability is not included in the requirements specified in the current international standard covering root canal sealers. A number of different in vitro methods have been used to evaluate the sealing quality of endodontic filling materials. The tests have usually been leakage tests, i.e. based on assessment of penetration of a tracer along the obturated root canal. Leakage tests have poor reproducibility and are, therefore, not suited for standardized test programs. By selecting the properties necessary for sealing ability and testing them separately, it might be possible to find a number of tests, which individually are suitable for a standardized test program, and which combined will give information on the sealing ability of the test material.

Animals↗

Coronal sealing ability of three sectional obturation techniques--SimpliFill, Thermafil and warm vertical compaction--compared with cold lateral condensation and post space preparation.

The purpose of this study was to evaluate the effect post space preparation has on the coronal seal of root canals obturated with cold lateral condensation of gutta-percha compared with the corsonal sealing ability of three sectional obturation techniques not requiring post space preparation. Sixty-eight human maxillary central incisors were divided equally into four groups: cold lateral condensation (Group I), SimpliFill (Group II), Thermafil (Group III) and warm vertical compaction (Group IV). After coronal flaring, all four groups were cleaned and shaped with Lightspeed rotary instruments to a size 60 master apical rotary. After obturation Peeso Reamers were used to create a post space for Group I, while Groups 2, 3 and 4 incorporated the post space in the obturation (sectional technique) and did not require making a post space after obturation. The teeth were then stored in 100% humidity for 1 week and then kept in rhodamine B fluorescent dye for 3 weeks. The teeth were then split open, and the linear dye penetration from a coronal to apical direction (coronal seal) was evaluated with a fluorescent light microscope. Statistical analysis showed that Group I (cold lateral condensation followed by post space made with Peeso Reamers) leaked significantly more (P < 0.05) than the remaining three sectional obturation groups. It was concluded that stresses generated during post space preparation might be detrimental to the seal obtained by the obturation. Sectional obturations with their superior sealing ability offer a viable alternative.

Dental Leakage↗

Dynamics of membrane sealing in transient electropermeabilization of skeletal muscle membranes.

Large supraphysiologic transmembrane electrical potentials are known to alter the molecular organization of the bilayer lipid component of cell membranes, leading to ionic permeabilization or "electroporation". Typically, membrane electroporation is followed by several orders of magnitude increases in electrical conductance and diffusive permeability to low-molecular-weight solutes. Electroporation may be transient or stable depending on whether the membrane eventually seals or remains permeabilized. Factors that control sealing have not been well characterized. This paper describes the kinetics of membrane sealing following electroporation by pulses over a range of supraphysiologic potentials. The increase in membrane conductance is highly nonlinear during a -440-mV, 4-ms pulse and reaches two orders of magnitude greater than baseline. Electroporation and relaxation sealing kinetics are quite different, reflecting a significant hysteresis effect. Thus, it appears that the magnitude and duration of the field pulse are important factors in sealing.

Animals↗

Serum chemistry values for free-ranging ringed seals (Pusa hispida) in Svalbard.

BACKGROUND: Diseases and abnormal physiologic conditions can alter the concentrations of enzymes, metabolites, minerals, and hormones in the blood of animals. The ringed seal (Pusa hispida) has been selected as a key species for environmental monitoring, but information on disease and health parameters for this species is scarce. OBJECTIVES: The aim of the study reported here was to obtain serum chemistry reference intervals for free-ranging ringed seals in Svalbard, and then to evaluate serum chemistry values in relation to age, body condition, and sex. METHODS: Blood samples were collected after death from ringed seals in Wijdefjorden and Billefjorden, Svalbard (2002-2003; n = 75). Serum was analyzed for 24 selected serum chemistry parameters (enzymes, protein, metabolites, minerals, and cortisol). RESULTS: Compared with younger or older animals, seals between 7 and 16 years of age had larger variations in the activities of alanine transaminase, aspartate transaminase, lactate dehydrogenase, and creatine kinase (CK). Animals classified as having low body condition status had more variation in the serum activity of these enzymes, compared with that in animals with higher condition scores. Serum cortisol concentration was higher in young animals (1-5 years) than in older animals. Serum CK activity was higher in males than in females. CONCLUSION: The data reported here may be useful in monitoring the health of ringed seals and for tracking the impact of environmental changes in the Arctic.

Aging↗

Underwater auditory localization by a swimming harbor seal (Phoca vitulina).

The underwater sound localization acuity of a swimming harbor seal (Phoca vitulina) was measured in the horizontal plane at 13 different positions. The stimulus was either a double sound (two 6-kHz pure tones lasting 0.5 s separated by an interval of 0.2 s) or a single continuous sound of 1.2 s. Testing was conducted in a 10-m-diam underwater half circle arena with hidden loudspeakers installed at the exterior perimeter. The animal was trained to swim along the diameter of the half circle and to change its course towards the sound source as soon as the signal was given. The seal indicated the sound source by touching its assumed position at the board of the half circle. The deviation of the seals choice from the actual sound source was measured by means of video analysis. In trials with the double sound the seal localized the sound sources with a mean deviation of 2.8 degrees and in trials with the single sound with a mean deviation of 4.5 degrees. In a second experiment minimum audible angles of the stationary animal were found to be 9.8 degrees in front and 9.7 degrees in the back of the seal's head.

Acoustics↗

Control of membrane sealing in injured mammalian spinal cord axons.

The process of sealing of damaged axons was examined in isolated strips of white matter from guinea pig spinal cord by recording the "compound membrane potential," using a sucrose-gap technique, and by examining uptake of horseradish peroxidase (HRP). Following axonal transection, exponential recovery of membrane potential occurred with a time constant of 20 +/- 5 min, at 37 degrees C, and extracellular calcium activity ([Ca(2+)](o)) of 2 mM. Most axons excluded HRP by 30 min following transection. The rate of sealing was reduced by lowering calcium and was effectively blocked at [Ca(2+)](o) </= 0.5 mM, under which condition most axons continued to take up HRP for more than 1 h. Sealing at higher [Ca(2+)](o) was blocked by calpain inhibitors (calpeptin and calpain inhibitor-1) indicating a requirement for type II (mM) calpain in the sealing process. Following compression injury, the amplitude of the maximal compound action potential conducted through the injury site was reduced. The extent of amplitude reduction was increased when the tract was superfused with calcium-free Krebs' solution (Ca(2+) replaced by Mg(2+)). These results suggest that the fall in [Ca(2+)](o) seen following injury in vivo is sufficient to prevent membrane sealing and may paradoxically contribute to axonal dieback, retrograde cell death, and "secondary" axonal disruption.

Action Potentials↗

Testing sealing properties of restorative materials against moist dentin.

The objectives of this investigation were (1) to develop a sensitive laboratory system that simulates the physiological and clinical conditions of a prepared human tooth in order to facilitate the determination of the bonding and sealing efficacies of restorative dental materials and (2) to conduct experiments with two dentin bonding agents, two posterior composite resin restorative materials (hybrid and microfilled), and a glass-ionomer type II filling material. The system functioned well and provided data to indicate that, after margination, the two posterior composite resin restorative materials performed equally and both performed better than the glass-ionomer filling material; none of the materials provided a perfect seal immediately after insertion, although some restorations demonstrated perfect seals from 16 hours to 28 days after placement; the seal of the glass-ionomer material improved after 28 days of storage in buffer solution; each successive step in the respective composite resin procedures improved the seal except in one case for the microfilled resin.

Composite Resins↗

Adapting to the challenge of psychosis: personal resilience and the use of sealing-over (avoidant) coping strategies.

BACKGROUND: Avoidance coping (e.g. sealing over) is common in people recovering from psychosis, but it is not understood why some individuals 'seal over'. AIMS: We examined the hypothesis that individuals who 'seal over' do not have the personal resilience to withstand this major life event. METHOD: Fifty participants were interviewed during an acute episode of psychosis and reassessed at 3-month and 6-month follow-up. Measures included psychotic symptoms, recovery style, service engagement, parental and adult attachment and self-evaluative beliefs. RESULTS: Sealing-over recovery styles are associated with negative early childhood experience, insecure adult attachment, negative self-evaluative beliefs and insecure identity. Insecure adult attachment was associated with less engagement with services. CONCLUSIONS: Sealing over was associated with multiple signs of low personal resilience in adapting to psychosis.

Adaptation, Psychological↗

Developmental changes in cardiorespiratory patterns associated with terrestrial apnoeas in harbour seal pups.

During the nursing period seals undergo several physiological and behavioural changes. A key component of development is increased cardiorespiratory control, fundamental for breath-holding and thus diving. This study focused on the ontogenetic changes in cardiac responses to respiration in quietly resting, pre-weaned harbour seal pups (Phoca vitulina). During periods of quiet rest, breathing became episodic, eupnoea interspersed with periods of apnoea. Little change was observed in respiration (approximately 35 breaths min(-1)) and eupnoeic heart rate (approximately 160 beats min(-1)) throughout the nursing period. However, apnoea duration increased (from approximately 20 to 40 s), while apnoeic heart rate decreased with age (from approximately 150 to 90 beats min(-1)). The observed decline in apnoeic heart rate resulted from an increase in cardiorespiratory control as pups approached weaning, evident by the ability to maintain a lower heart rate more consistently. Similar changes in cardiorespiratory patterns have been reported for elephant and Weddell seals. Due to the early onset of independent foraging, however, the rate of cardiorespiratory control development was more rapid in harbour seals. Our findings suggest that by 1 month of age, harbour seal pups possess the cardiorespiratory control necessary to sustain long-duration apnoeas, fundamental for proficient diving and successful foraging upon weaning.

Age Factors↗