Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “RADIOAUTOGRAPHY”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 541 records · Page 30Linked to original sources
PubMed1967

Radioautography of noradrenaline-14-C in atopic dermatitis.

Since storage-time of administered noradrenaline in the skin of patients with atopic dermatitis may be prolonged, it would be of interest to demonstrate the site of uptake of noradrenaline-(14)C in atopic dermatitis as compared with other eczematous and normal skins. Two adult patients with longstanding atopic dermatitis, a patient with contact dermatitis to nickel and one with normal skin, were studied. Identical sites in the four patients were injected intradermally with 0.02 mug. DL-noradrenaline-7-(14)C acetate. An 8-mm. punch biopsy of the injected site was performed 24 hours later. Radioautographs were developed between three and 199 days, according to the technique of Kopriwa and Leblond.(2) At 199 days, the number of grains in atopic dermatitic skin was greater than in contact dermatitis or normal skin. There was a concentration of grains over arrectores pilorum muscles and the upper one-third of the epidermis of atopic skin. Grains were also visible in proximity to arteriolar walls. There were few grains visible in the control sections. The results confirm earlier studies suggesting that atopic dermatitic skin retains noradrenaline longer than other dermatoses. Noradrenaline concentrates in the arrectores pilorum muscles and the upper epidermis. These findings may explain the cutis anserina (goose-flesh) appearance in atopic dermatitis.

Adult↗
PubMed1978

Fine structure and radioautography of rabbit photoreceptor cells.

The retina of the adult rabbit contains two types of photoreceptors. Type I predominates and is a typical rod. The identity of the type II cell has been uncertain until now. This study demonstrates that the type II cell has certain conelike morphologic features including a pale nucleus, complex synaptic pedicel, and multiple wrappings of the outer segment by microvilli of the pigment epithelium. The type II outer segment is also conelike in that some intradisc spaces are patent to the extracellular space, that the pattern of renewal is diffuse as demonstrated radioautographically with sugar and amino acids as precursors, and that the few examples of distal tip shedding found in this study occur mainly in the early part of the dark cycle. The interior of the type II outer segment is labeled selectively with 3H-fucose, whereas the interior of the type I outer segment remains unlabeled with this sugar at all time intervals examined. The label probably reflects the presence of a fucose-containing glycoprotein in the conelike outer segment which is lacking in the rod outer segment and may represent a visual pigment or other intrinsic molecule(s). An amorphous material which forms a symmetric sleeve around the photoreceptor connecting cilium has been demonstrated after intravitreal injections of horeseradish peroxidase. This cilium-associated material is labeled specifically by 4 hr following intravitreal injection of 3H-fucose, before the interphotoreceptor matrix shows significant labeling, suggesting that it is composed of a sugar-containing material. Possible functions of the cilium-associated material, including structural support and ionic regulation, are discussed.

Animals↗
PubMedDate not supplied

[Use of highly sensitive radioautography for determining the optimal conditions for obtaining hybridomas].

To detect the hybrid cells forming as a result of fusion of the mouse myeloma cells with the immunic mouse splenocytes, a radioautographic method was used which involved the application of 3H-hypoxanthine as a labelled precursor of nucleic acids and of high-sensitive UK emulsion for accelerated preparations of autographs. The optimal conditions of hybridization providing for the maximum yield of hybrid cells and hybrid clones in our experiments were: use of polyethylene glycol ("Löba", mol. weight 4000) in 50% (w/v) concentration, of NS-O or X-653 myeloma cells in the ratio of parental cells 1:5 (myeloma cell: splenocyte).

Animals↗
PubMed1982

The effects of vinblastine on the cell structure and activity in the rat incisor enamel organ during the secretory stage in vivo as shown by radioautography using 3H-proline, 3H-serine and 3H-fucose.

Four hours after injection of vinblastine sulphate (VBL) the cells of the enamel organ of the rat incisor showed: 1) loss of polarity in the tall secretory ameloblasts--secretory granules, which were lacking in the Tomes processes accumulated in the supranuclear area; condensing vacuoles also accumulated near dictyosomes; enlarged intercellular spaces without disruption of intercellular junctions; lysosomal structures and autophagic vacuoles. 2) an increase of lysosome-like structures in the outer enamel epithelium, the stellate reticulum and the stratum intermedium. 3) a severe reduction in number of free ribosomes in the stratum intermedium as well as an increase in size of the Golgi complex: numerous electron lucent vesicles appeared. These findings as well as radioautographic data demonstrated 1) the impairment of secretory activity of ameloblasts. 2) the restriction of permeability in the enamel organ suggesting strong interaction between VBL and the membranes.

Ameloblasts↗
PubMed1980

Fine structure and radioautography of retinal cone outer segments in goldfish and carp.

Cone but not rod outer segments (OSs) in the retinas of goldfish and carp are diffusely labeled after intravitreal injection of 3H-fucose. Among the different cone types, the OSs are heavily labeled in the long double, short single, and some long single cones. The OSs of short double and the remaining long single cones are consistently more lightly labeled. A correlation thus exists between heavy labeling of OSs maximally sensitive to red and blue light and lighter labeling of OSs with maximum sensitivity to green light, and this may reflect different carbohydrate compositions of the cone visual pigments. New information is presented on OS fine structure of rods and cones, and on differential marking of cone OSs with the extracellular tracers horseradish peroxidase (only green-sensitive cone OSs "stained") and microperoxidase (red- and blue- but not green-sensitive cones OSs "stained").

Animals↗
PubMed1980

Glucocorticoid localization by radioautography in the rabbit eye following systemic administration of 3H-dexamethasone.

Dexamethasone was localized radioautographically in the nuclei of target cells of the rabbit eye following intravenous administration of the labeled steroid. Specifically bound steroid was found in the nuclei of stromal and endothelial cells of the outflow pathway region. This suggests that the glucocorticoid-induced alteration in outflow facility may be mediated by specific effects in these target cells. Nuclear localization was also found in conjunctiva, iridial smooth muscle, choroidal stroma, retina, and sclera, suggesting a physiologic role for glucocorticoids in these tissues as well.

Animals↗
PubMed1981

Ultrastructural cytochemistry and radioautography of complex carbohydrates in secretory granules of epiphyseal chondrocytes.

Secretory granules of chondrocytes contain a variety of complex carbohydrates, which include glycosaminoglycans and glycoproteins necessary for the formation of the cartilage matrix. The present study has utilized ultrastructural, cytochemical, and radioautographic methods to determine the subcellular route of incorporation of sulfated and vicinal glycol-containing complex carbohydrates into secretory granules and their subsequent distribution in granules. High iron diamine specifically stained sulfated glycoconjugates in Golgi saccules, Golgi derived vacuoles, and mature secretory granules. Incubation of chondrocytes with 35SO4 resulted in significant radioautographic labeling of Golgi saccules, condensing vacuoles, intermediate granules containing moderately dense amorphous material, and mature granules containing fibrillar material. Periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) staining of unosmicated thin sections localized vicinal glycol-containing glycoconjugates in cisternae of rough endoplasmic reticulum and coated vesicles, some of which appeared to be budding from rough endoplasmic reticulum. Similar coated vesicles appeared to fuse with condensing vacuoles and granules of intermediate maturity which evidenced moderate PA-TCH-SP reactivity. These vesicles were very seldom seen associated with Golgi saccules which contained 35SO4 radiolabels and high iron diamine reactivity but lacked significant PA-TCH-SP staining. The PA-TCH-SP-positive vesicles were not associated with mature secretory granules which demonstrated strong PA-TCH-SP staining. Lysosomes demonstrated moderately heavy PA-TCH-SP staining. Amylase digestion of en bloc specimens removed all PA-TCH-SP reactive cytoplasmic glycogen, however, staining of cytoplasmic organelles was not decreased. These studies indicate that sulfated glycosaminoglycans and vicinal glycol-containing glycoproteins are transported to secretory granules by Golgi saccules and coated vesicles, respectively, and are subsequently distributed differently within the maturing secretory granules.

Animals↗