Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “PASTEURELLA”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 541 records · Page 30Linked to original sources
PubMed1979

Serologic study of Pasteurella aerogenes sp n.

The antigenic attributes of Pasteurella aerogenes sp n were serologically compared with species of the genera Actinobacillus and Pasteurella. Examination included the tube-agglutination and double-immunodiffusion techniques. The results indicated the possibility of serologically different strains of P aerogenes. Antisera prepared from strains of P aerogenes also reacted well with antigens prepared from Yersinia pseudotuberculosis (P pseudotuberculosis) and P pneumotropica.

Actinobacillus↗
PubMed1979

[Decarboxylase activity study of Pasteurella multocida].

Ornithine-, lysine- and arginine-decarboxylase activity of 218 P. multocida strains, isolated from birds of varying disease symptoms in Bulgaria and CSSR, and from pigs, rabbits and birds in Cuba, USSR and CSSR, was studied after the method of Moller. Positive ornithine decarboxylase activity was established in 211 strains, low -- in 2, and negative -- in 5 strains. Low arginine decarboxylase activity was observed in 12 Pasteurella strains, while in 14 -- low lysine decarboxylase activity. The presence of ornithine decarboxylase activity can be used, along with the cultural and biochemical properties and with lyzation by a specific bacteriophage, as a taxonomic character for the species. All Pasteurella strains pathogenic for white mice, produce ornithine-decarboxylase. Lines of the strain X 73 obtained following gamma-irradiation having lost their ornithine-decarboxylase are pathogenic for white mice.

Animals↗
PubMed1975

[Study of Dermanyssus gallinae as a carrier of Pasteurella multocida].

Microbiologic studies and biologic experiments revealed that Pasteurella multocida persists in the body of Dermanyssus gallinae mites after these engorge with blood from infected birds. Depending on the temperature of the environment the carrier status was shown to last from 42 to 64 days. It is reported that the red mite acts as a vector and does not transmit Pasteurellae directly. However, the parasite is potentially hazardous in maintaining and passing on the infection through other indirect routes. Carrier status has been established in naturally infected Dermanyssus gallinae mites.

Animals↗
PubMed2002

Coinfection with BHV-1 modulates cell adhesion and invasion by P. multocida and Mannheimia (Pasteurella) haemolytica.

Viruses are thought to facilitate bacterial infections of the respiratory tract. The present study shows the effect of BHV-1 on Pasteurella multocida and Mannheimia haemolytica adherence and invasion of MDBK cells. The virus-infected MDBK cells become more susceptible to the adherence of both species of Pasteurella. The observed adherence increase depends on the length of virus pre-incubation time and on virus concentration. When MDBK cells are not infected with virus, they are only invaded by P. multocida, while M. haemolytica is not able to penetrate. The viral infection favours also the invasion by M. haemolytica.

Animals↗
PubMed1992

Colonization of the tonsils of calves with Pasteurella haemolytica.

Tonsils of 10 calves were inoculated with Pasteurella haemolytica (PH) and the degree of colonization was followed by collecting sequential tonsil. wash specimens. Tonsils were colonized for at least 3 weeks after instillation of PH into the tonsillar sinus. Calves with colonized tonsils responded with serum and nasal secretion antibody responses to PH and to leukotoxin. Pasteurella haemolytica was detected in nasal mucus specimens of 2 calves during the week after inoculation of the tonsils, but all other specimens were culture-negative. Infectious bovine rhinotracheitis virus-induced respiratory tract disease 25 days later did not elicit a population increase of PH in the tonsils, and did not elicit shedding of PH in nasal mucus.

Animals↗
PubMed1991

[Isolation and certain properties of the dermonecrotic toxin from Pasteurella multocida].

The procedure for isolation and purification of Pasteurella multocida serovariant D toxin has been described. It includes the three steps of protein precipitation from cultural filtrates by 70% ammonium sulfate, chromatography of the concentrated material on Ultragel AcA44 gel-filtration on Sephracryl S-200. The proposed technique permits one the 155-fold purification of the preparation with 32.6% yield estimated by biological activity. The obtained purified preparation is homogeneous in polyacrylamide gel electrophoresis. The immunological methods also confirm the homogeneity of the preparation. The minimal dermonecrotic dose for guinea pigs of the purified 120 kDa toxin is 78 ng and LD50 for mice is 280 ng. Pasteurella multocida toxin is found to be a thermolabile protein sensitive to trypsin, glutaraldehyde and formaldehyde treatments.

Animals↗
PubMed1977

[Pasteurella multocida bacteriophage adsorption].

Studied was the adsorption of 8 strains of Pasteurella multocida bacteriophages presenting three groups of phages isolated in Bulgaria. With the bacteriophages of groups I and II the adsorption in broth in the first 10 minutes amounted to 50-65%, and at the 80 the minute reached its maximum value--98-99%. With the bacteriophages of group III adsorption started with a lower percent and by the 140th minute reached 96-97%. It was by 60 min slower as compared with the adsorption with group I and II phages. In a veronal-medinal buffer the adsorption of all investigated phages of the three groups set in with a prolonged time of 60 min as compared with the time elapsed using broth. At the cross testing of adsorption the phages of group I were adsorbed by the hosts of the other two groups to a maximum level, however, the phages of groups II and III were adsorbed to a negligible extent on the other hosts (except for the adsorption on their homologous one). The difference established in the adsorption for a 60 minutes time so far as the phages of group III were concerned was sufficiently persuasive to consider this group as an individual one by this feature too. Adsorption appeared as a subsidiary taxonomic characteristic in the classification of Pasteurella multocida bacteriophages.

Adsorption↗
PubMed1991

Determination of affinity of Pasteurella multocida isolates for porcine respiratory tract mucus, and partial characterization of the receptors.

The ability of 25 Pasteurella multocida isolates to adhere in vitro to porcine respiratory tract mucus was examined. Microplate wells were coated with crude mucus preparation, then bacteria were added. After incubation, unbound bacteria were removed by washing, and the number of mucus-bound bacteria was estimated by quantitation of the adherent colony-forming units and by use of an ELISA. Pasteurella multocida had affinity to respiratory tract mucus, although significant differences were not observed in affinity of capsular type-A and type-D isolates. Preliminary characterization, using ultrafiltration, gel filtration chromatography, electrophoresis, and enzymatic treatments, indicated that the receptors may be a class of protein molecules of low molecular weight (less than 25,000). The origin of these receptors, however, is not known at this time.

Animals↗
PubMed1990

Pasteurella haemolytica lipopolysaccharide-induced arachidonic acid release from and neutrophil adherence to bovine pulmonary artery endothelial cells.

Bovine pulmonary artery endothelial cells (BPAEC) were labeled with 3H-arachidonic acid. Exposure of the labeled BPAEC to Pasteurella haemolytica lipopolysaccharide (LPS) resulted in a time- and dose-dependent release of radioactivity. The release was inhibited by 5 mM indomethacin, but inhibition was not caused by less than or equal to 500 microM indomethacin or hydrocortisone, which suggests that the release was caused primarily by a mechanism other than cyclooxygenase or phospholipase A2 metabolism of arachidonic acid. Pasteurella haemolytica LPS also caused increased adherence of bovine neutrophils to BPAEC through independent effects on both cell types. The increased adherence was inhibited by treatment of either cell type with cycloheximide or actinomycin D prior to LPS exposure, indicating that de novo protein synthesis was required in both cell types to promote the LPS-induced adherence. Lipopolysaccharide may be an important factor in neutrophil-mediated effects in pneumonic pasteurellosis by causing increased neutrophil adherence and, thus, the vascular sequestration of neutrophils. Together, these experiments provide additional evidence for the involvement of LPS in pneumonic pasteurellosis. Moreover, they provide evidence of LPS-induced endothelial activation, which could have broad ramifications in the inflammatory and immune responses of pneumonic pasteurellosis.

Animals↗
PubMed1990

Distribution of Pasteurella haemolytica in the respiratory tracts of carrier calves and those subsequently infected experimentally with Dictyocaulus viviparus.

Distribution of Pasteurella haemolytica in the respiratory tracts of calves with no apparent clinical signs of illness and those infected experimentally with Dictyocaulus viviparus was determined so as to define carrier sites for this organism. The calves had been positive by nasopharyngeal swab for either P haemolytica A2 or A1 for at least two months or for over a month, respectively, before slaughter. P haemolytica A1 was acquired following horizontal spread from other infected calves. It was observed post mortem that P haemolytica A1 or A2 resided in the tonsils and retropharyngeal lymph nodes of calves of both groups. In addition to these sites, P haemolytica A1 was also isolated from the right cranial lung lobe of one of the calves from the D viviparus infected group although there was no evidence of pasteurella associated pneumonia. It was concluded that tonsil and retropharyngeal lymph nodes appear to be the most important carrier sites for P haemolytica when compared to other tissues of the bovine respiratory tract.

Animals↗
PubMed1990

Comparison of DNA:DNA homology and enzymatic activity between Pasteurella haemolytica and related species.

A commercially available microbiological identification system and DNA:DNA hybridization were used to determine relationships between and within serovars 1-13 of Pasteurella haemolytica, and between P haemolytica and P multocida and 4 species of Actinobacillus. All serovars of P haemolytica that belonged to biovar A were related with mean DNA homology of 78%, whereas all serovars of P haemolytica that belonged to biovar T were related to each other with mean DNA homology of 90%. The DNA:DNA hybridization between strains of biovars A and T ranged from 3 to 13%, indicating little or no genetic relationship between the 2 biovars of P haemolytica. The DNA homology between all serovars of P haemolytica and other species of non-P haemolytica bacteria tested (P multocida and actinobacilli) was less than 14%, suggestive of essentially no genetic relationship of P haemolytica with the ATCC reference strains of the genus Pasteurella or the genus Actinobacillus. Enzymatic differences were observed between P haemolytica and the other non-P haemolytica bacteria tested; however, the microbiological identification system that uses enzymatic reactions could not distinguish among biovars of P haemolytica. Results of this research support other data that suggest that biovars A and T of P haemolytica should be classified as separate species, but do not support the inclusion of either biovar A or T within the genus Actinobacillus.

Acid Phosphatase↗
PubMed1990

[Epizootiology, clinical aspects and therapy of Pasteurella multocida infection in bird patients after cat bite].

Since early 1986, fatal cases of Pasteurella multocida infection following cat bites have been seen regularly among avian patients at our institute. The problems of the epizootiology of this infection are discussed on the basis of research during the last three and a half years. By evaluation of collected data from clinical examinations, autopsies, bacteriological and biochemical diagnosis of isolated strains from birds and cats the possibility of transmission via cat bite is discussed as well as the status of cats and birds in an epizootic chain. Even birds with trivial wounds caused by cats must be classified as emergency patients. The risk of an infection after a cat bite is about 56%. For treatment of a Pasteurella multocida infection Doxycycline should be used.

Animals↗
PubMed1990

Pasteurella multocida empyema: successful treatment with open thoracostomy.

Pasteurella multocida appears to be an uncommon pathogen in human thoracic empyema. The morbidity and mortality associated with these infections has been significant, presumably secondary to the elderly populations they affect, many with chronic lung disease and impaired pulmonary defenses. We report a case of pasteurella empyema treated with open thoracostomy and rib resection and advocate use of such a procedure early in the treatment of patients with this infection.

Aged↗
PubMed1990

Effect of Pasteurella haemolytica-derived endotoxin on pulmonary structure and function in calves.

The role of endotoxin in the pathogenesis of acute pneumonic pasteurellosis is uncertain. Recently, we reported that Escherichia coli-derived endotoxin given by airway inoculation fails to induce lung injury in calves. Because Pasteurella haemolytica-derived endotoxin may differ substantially from E coli in its pathogenicity, we repeated these studies with Pasteurella endotoxin. Intratracheal inoculation of P haemolytica endotoxin caused hypoxemia and increased the alveolar-arterial oxygen differences without causing hypercarbia or changes in lung mechanical properties and volumes. In contrast, IV inoculation of endotoxin caused systemic hypotension, leukopenia, gas exchange impairment, increased total pulmonary resistance, and decreased dynamic compliance. Both routes of inoculation increased serum endotoxin concentrations and were associated with areas of pulmonary hemorrhage, edema, and acute inflammation. We concluded that P haemolytica-derived endotoxin is pathogenic by IV and airway routes of inoculation, and therefore differs from E coli endotoxin in its ability to induce lung lesions in calves.

Animals↗
PubMed1990

Effects of Pasteurella haemolytica A1 culture supernatant on mechanisms controlling bovine alveolar macrophage oxygen radical production.

Pasteurella haemolytica A1 culture supernatant containing leukotoxin, and modifiers of cyclic nucleotide and arachidonate metabolism, were evaluated for their ability to alter oxygen radical production by pulmonary alveolar macrophages obtained from seven Holstein calves. Calves were sedated, and underwent bronchoalveolar lavage to harvest macrophages, which were then incubated with culture supernatant and/or the drugs and toxins under study, and challenged with opsonized zymosan to induce oxygen radical generation. This was measured by a chemiluminescence technique. Pasteurella haemolytica A1 culture supernatant alone delayed the time to maximum oxygen radical production, although total production was increased. The cyclooxygenase inhibitor indomethacin and the phospholipase inhibitor p-bromophenacyl bromide significantly reduced maximum oxygen radical production, but their effects were diminished in the presence of culture supernatant. Although forskolin markedly inhibited oxygen radical generation, this effect was not altered by culture supernatant. Incubation of macrophages with pertussis toxin had no effect on oxygen radical production, while incubation with cholera toxin did inhibit production. This inhibitory effect was significantly lessened by concurrent incubation with P. haemolytica A1 culture supernatant.

Acetophenones↗
PubMed1988

Antigenic analysis of Pasteurella haemolytica serovars 1 through 15 by crossed immunoelectrophoresis.

Pasteurella haemolytica serovars 1 through 12, grown in broth and on agar plates, and 2 field isolates (types A1 and T10) were used to develop polyvalent crossed immunoelectrophoresis (XIE) reference systems. The maximal number of antigens was revealed by XIE when sonicates of agar plate-grown organisms were used as the immunogen (to produce antibodies) and as the soluble antigen for XIE. Antigens produced from agar plate-grown organisms were less contaminated (by antigenic components of the medium) than were those produced from organisms grown in broth. Seventy-two antigens were detected in sonicated preparations of agar plate-grown P haemolytica. The common antigen of gram-negative bacteria was identified in the P haemolytica XIE reference system; precipitation was observed with rabbit antiserum to the common antigen of gram-negative bacteria isolated from Escherichia coli, as well as with rabbit immunoglobulins (obtained from unvaccinated rabbits). Most preimmune sera from our vaccinated rabbits also precipitated the common antigen. Serovar-specific antigens in the P haemolytica XIE reference system were defined and presumptively identified as part of the bacterial lipopolysaccharide complex by use of the limulus amebocyte lysate test. Partial cross-reactions were found between serovar-specific antigens within each biovar (A and T). Pasteurella haemolytica biovar A-specific and biovar T-specific antigens were defined by crossed-line immunoelectrophoresis. When serovars A13, A14, and T15 were tested in the P haemolytica XIE reference system, they gave high matching coefficient values of 0.98, 0.98, and 0.87, respectively. The proposal to separate P haemolytica biovars A and T into 2 different species was supported by immunotaxonomic data obtained from crossed immunoelectrophoresis, but more extensive studies will be necessary to establish the appropriate taxonomic position of these 2 groups of organisms.

Animals↗
PubMed1989

[Corneal abscess caused by Pasteurella following cat scratch injury].

Authors report a Pasteurella Multocida corneal abscess after a scratch from a cat. The evolution was torpid and needed a transfixiant keratoplasty. They recall bacterial tanks, infestation ways, clinical manifestations and bacteriological identification. They note the low occurrence of ophthalmological manifestations and the sensibility of Pasteurella to various antibiotics, especially Beta lactams, phenicol and cyclins.

Abscess↗
PubMed1989

[A case of chronic bronchitis with Pasteurella multocida possibly resulting from infection from a bird].

A 60-year-old woman was admitted to our division for further evaluation of fever and purulent sputa. In sputum cultures performed when the patient had complained of an increase in symptoms on three occasions during the previous 6 months, Pasteurella multicoida was usually detected. Based on the fact that the bacteria had been detected from the patient's sputa after feeding a macaw, but was not detected after treatment of the bird with OFLX, a diagnosis of respiratory tract infection by P. multocida was made. Clinical symptoms and laboratory data of the patient were markedly improved after treatment with cefaclor (750 mg/d). The bacteria in this case were sensitive toward many antibiotics. This case was considered to be the first case of bird-mediated Pasteurella infection.

Animals↗