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At least 541 records · Page 30Linked to original sources
PubMed1999

Electrophoretic monitoring of pollutants: effect of cations and organic compounds on protein interactions monitored by native gel electrophoresis.

We describe how the interaction between actin and its protein ligands can be used to evaluate the presence of certain metal (Cd, Cu, Hg, Zn) ions and organic compounds (2,4-dioxin or Picloram) which are common components of environmental pollution. The assay detects the high-affinity binding of actin to actin-binding proteins (ABPs), cofilin or DNase I. The actin-ABP complex was analyzed using native polyacrylamide gel electrophoresis and quantified by scanning densitometry. These proteins are widely distributed in animals and plant cells. The assay involves allowing the proteins to form an actin-ABP complex into which increasing amounts of pollutants are titrated. Thus, the assay directly tests for inhibition of protein-protein interaction. It is sensitive to common pollutants using concentration ranges over which they are known to exert a biological toxicity. A convenient feature of the assay is the fact that all the proteins can be stored in freeze-dried form, and can be purchased commercially. We suggest that if this molecular assay is sensitive to a wide range of environmental pollutants, it could be used as a rapid and convenient assay of the environment in combination with currently available tests.

Actin Depolymerizing Factors↗
PubMed1985

Multiple unipolar lead electrocardiographic monitoring during exercise in severe coronary artery disease: a comparison with bipolar lead monitoring.

A system of 21-lead electrocardiography was used to assess 21 patients with severe angina during and after exercise using on-line computerised ST segment analysis. A direct comparison was made between the results obtained from 18 unipolar precordial leads and those from bipolar leads CM5 and CC5. Treadmill exercise was performed 48 hr prior to cardiac catheterization, which revealed luminal narrowing of at least 70% in one or more major coronary arteries in all cases. In all cases the ST depression exceeded 1 mm in both CM5 and CC5 at the peak of exercise. The magnitude of ST depression was greater in the bipolar leads in 75% of cases and in the remaining 25% the greatest peak ST depression occurred in a single unipolar lead. There was no correlation between the magnitude of ST depression and the number of coronary vessels involved. Isopotential surface mapping in the anterior, lateral and inferior projections from the unipolar leads at each stage of exercise failed to show a correlation between the area or distribution of ST segment change and the number or anatomical location of the vessels involved. It was not possible to show that the multiple-lead system could differentiate the site and severity of coronary artery disease in these patients with angina. The multiple-lead system was cumbersome and time-consuming in application and therefore cannot be recommended for routine exercise testing.

Aged↗
PubMed1993

Quality assurance of ultrasound imaging instruments by monitoring the monitor.

Ultrasound quality assurance (QA) is a means of assuring the constant performance of an ultrasound instrument. A novel 'ultrasound image analyser' has been developed to allow objective, accurate and repeatable measurement of the image displayed on the ultrasound screen, i.e. as seen by the operator. The analyser uses a television camera/framestore combination to digitize and analyse this image. A QA scheme is described along with the procedures necessary to obtain a repeatable measurement of the image so that comparisons with earlier good images can be made. These include repositioning the camera and resetting the video display characteristics. The advantages of using the analyser over other methods are discussed. It is concluded that the analyser has distinct advantages over subjective image assessment methods and will be a valuable addition to current ultrasound QA programmes.

Humans↗