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PubMed1988

[Clostridium perfringens septicemia].

We report 3 cases of Clostridium perfringens bacteremia with uterine gas gangrene. Clinical presentation included severe infectious syndrome, hemoglobinemia and hemoglobinuria, jaundice, uterine tenderness and hypertension. All 3 cases were first seen with installed renal failure. Diagnosis and modalities of therapy were reviewed. Clostridium perfringens bacteremia with uterine gas gangrene still occur in developing countries.

Adult↗
PubMed2000

Diagnosis of neonatal pig diarrhea.

To be effective, swine practitioners should develop a unit health program. Development should involve unit managers, owners, and employees involved in day-to-day operations. Emphasis on training personnel and management to reduce disease and collection of accurate records is necessary. Routine diagnostics are needed to solve disease problems. Communication with laboratory personnel to ascertain what samples are needed for diagnosis of particular problems cannot be overemphasized. General diagnosis of disease problems outlined by Vinson can be similarly followed within the specifics of diarrheal problems within units. 1. Observe symptoms exhibited by pigs, i.e., huddling, fecal material around perineum, extreme thirst, etc. 2. Evaluate the degree of morbidity and potential production losses. 3. Analyze possible specific causes of symptoms, i.e., environmental cleanliness, affected litter distribution, age of affected neonates, and other populations affected. 4. Examine live animals, i.e., obtain serum samples from a random population, take rectal temperatures of affected neonates, and evaluate fecal pH. 5. Necropsy dead or dying pigs [that] appear to represent the problem. 6. Submit live pigs or appropriate tissues from necropsied pigs to a diagnostic laboratory. 7. Re-evaluate environmental conditions that may be contributing to the problem (remember, unit employees are a part of the pigs' environment). 8. Evaluate management procedures contributing to the disease problem, i.e., lack of adherence to all-in all-out, rapid turn-around decreasing cleaning time etc. Following this format and communicating with diagnosticians should provide for positive results for the producers both entities serve.

Animals↗
PubMed2004

In vivo characterization of Lactobacillus johnsonii FI9785 for use as a defined competitive exclusion agent against bacterial pathogens in poultry.

AIMS: To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. METHODS AND RESULTS: Specific pathogen-free chicks (1 day old) were dosed with a single oral inoculum of 1x10(9) CFU. Lactobacillus johnsonii FI9785 and 24 h later were challenged in separate experiments with S. Enteritidis (S1400, nalr) and E. coli O78:K80 (EC34195, nalr). There were no significant effects against S. Enteritidis whereas colonization of the small intestine by E. coli O78:K80 was reduced significantly. Both S. Enteritidis and E. coli colonized the caeca and colon to levels equivalent to control birds and there was no reduction in shedding as assessed by a semi-quantitative cloacal swabbing technique. Specific pathogen-free chicks (20 day old) were dosed with a single oral inoculum of 1x10(9) CFU L. johnsonii FI9785 and 24 h later were challenged with C. perfringens. A single oral dose of L. johnsonii FI9785 was sufficient to suppress all aspects of colonization and persistence of C. perfringens. CONCLUSIONS: Lactobacillus johnsonii FI9785 may be given to poultry for use as a competitive exclusion agent to control C. perfringens. SIGNIFICANCE AND IMPACT OF THE STUDY: Lactobacillus johnsonii FI9785 may be a valuable tool to control the endemic disease of necrotic enteritis, thereby reducing economic losses associated with reduced use of antimicrobials in the poultry industry.

Animals↗
PubMed2006

A "fishy" cough: hepatobronchial fistula due to a pyogenic liver abscess.

A hepatobronchial fistula is an anatomic communication between the liver parenchyma and the bronchial tree. Major causes of such fistulae include inflammatory conditions resulting from obstruction of the biliary tract and infectious processes, such as pyogenic liver abscesses, amoebiasis, and hydatid cysts. We report a rare case of a patient (with a chronic, recurrent hepatic abscess) who suffered a persistent, productive cough resulting from a hepatobronchial fistula.

Anti-Bacterial Agents↗
PubMed1978

Synergistic hemolysis phenomenon shown by an alpha-toxin-producing Clostridium perfingens and streptococcal CAMP factor in presumptive streptococcal grouping.

A new phenomenon of synergistic hemolysis by Clostridium perfringens alpha-toxin and the streptococcal CAMP factor on human and guinea pig erythrocytes is described. A possible mode of action of the CAMP factors is suggested. On human blood agar all of the tested isolates of group B streptococci gave an arrowhead-shaped zone of hemolysis; 74% of group A gave a crescent-shaped lytic zone, whereas all isolates of groups C and G and the remaining 26% of group A streptococci gave a bullet-shaped lytic zone. By comparison, in the CAMP test incubated aerobically and anaerobically, 70 and 91%, respectively, of streptococci other than group B gave positive, arrowhead-shaped lytic zones. If all intermediate positive reactions in the CAMP tests were read as negative after aerobic incubation, only 89% of group B streptococci would be properly identified. The synergistic hemolysis phenomenon, using an alpha-toxin-producing C. perfringens and human blood agar, provided a reliable test for presumptive identification of group B streptococci, with promising potential to differentiate in the same test group A streptococci from other groups.

Animals↗
PubMed2003

Late bacterial keratitis after implantation of intrastromal corneal ring segments.

We report a case of bacterial keratitis that occurred after implantation of intrastromal corneal ring segments (Intacs). The patient presented with decreased vision, inflammation, and stromal infiltrates localized at the extremity of an Intacs channel 3 months after surgery. Culture were positive for Clostridium perfringens and Staphylococcus epidermidis. The infiltrates progressed despite treatment with topical fortified and systemic antibiotics. The Intacs were removed. The keratitis slowly resolved, and the patient recovered a best corrected visual acuity of 20/20.

Adult↗