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PubMed1993

A survey of the use of live animals, cadavers, inanimate models, and computers in teaching veterinary surgery.

A questionnaire, designed to gather information regarding the use of live animals, cadavers, inanimate models, and other innovative methods to teach veterinary surgery, was mailed to individuals in the surgery sections of all 31 veterinary schools in the United States and Canada. Of the 31 questionnaires mailed, 27 (87%) were completed and returned. Mean number of total, general, small-animal, and large-animal surgery laboratory sessions were 22, 5.9, 11, and 7.4, respectively. At 7 of 26 (27%) schools, animals were euthanatized prior to recovery from anesthesia in all teaching laboratories; in 18 schools (69%), small animals were euthanatized prior to recovery from anesthesia. In 4 (20%) of the 20 schools that offer large-animal laboratory courses, large animals were euthanatized prior to recovery from anesthesia. In 24 schools (88%), cadavers, models, or both were used in at least 1 laboratory session in their surgery training program. Models were used most frequently to teach suturing, knot typing, and hemostasis. Plastic bones were used in 8 (30%) schools to teach fracture repair. In several schools, models were used to teach other general psychomotor skills, and at several schools, models were available for sale to other teaching institutions. At 16 (59%) of the 27 veterinary schools, some type of program has been developed with local humane societies. At 13 (81%) of the 16 schools with such a program, small animals were euthanatized prior to recovery from anesthesia in their traditional laboratories. At 10 (37%) of the schools, some process was used to evaluate students in the laboratory, and at 5 (19%), course evaluations were completed by students taking the laboratory.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Welfare↗
PubMed1995

The infection hazards of human cadavers.

Cadavers may pose infection hazards to people who handle them. None of the organisms that caused mass death in the past--for example, plague, cholera, typhoid, tuberculosis, anthrax, smallpox--is likely to survive long in buried human remains. Items such as mould spores or lead dust are much greater risks to those involved in exhumations. Infectious conditions and pathogens in the recently deceased that present particular risks include tuberculosis, group A streptococcal infection, gastrointestinal organisms, the agents that cause transmissible spongiform encephalopathies (such as Creutzfeldt-Jakob disease), hepatitis B and C viruses, HIV, and possibly meningitis and septicaemia (especially meningococcal). The use of appropriate protective clothing and the observance of Control of Substances Hazardous to Health regulations, will protect all who handle cadavers against infectious hazards.

Autopsy↗
PubMed1976

HL-A and cadaver kidney transplantation. 7-year experience at Johannesburg General Hospital.

The role of HL-A matching in the clinical outcome of 159 consecutive first cadaver renal allografts at the Johannesburg General Hospital has been analysed over a 7-year period, April 1968-May 1975. Over-all actuarial graft survival was 69% at 1 year and 57% at 7 years. When all cases were considered together there was a trend towards improved graft survival with better grades of matching, but this was not statistically significant. Presensitisation had an adverse effect on graft survival. Donor-recipient combinations with no demonstrable FOUR locus mismatches provided a significantly superior graft survival rate of 82% 1-6 years after transplantation. The majority of these compatible for HL-A 7, 8 OR 12, and therefore may have been matched indirectly for lymphocyte activating determinants. Relatively inferior graft survival was observed with FOUR locus incompatibility in patients who had been presensitised, and when donor-recipient combinations had no serologically determined antigens in common, particularly in the donor "full house" situation. Despite these unfavourable immunogenetic circumstances, approximately 50% of grafts functioned well for long periods, and not infrequently clinical considerations took precedence over immunological considerations in the selection of recipients. This policy is justified by over-all results, the critical shortage of cadaver organs in relation to clinical requirements and the rare occurrence of combinations with superior HL-A matching. Specific allograft tolerance remains the goal of clinical organ transplantation.

Cadaver↗
PubMedDate not supplied

Wound strength comparison of a 5.1-millimeter no-stitch with a 7.0-millimeter sutured incision in human cadaver globes.

We compared the wound strength of 5.1-millimeter no-stitch incisions with that of 7.0-millimeter sutured incisions in cadaver eyes. Each incision was performed on seven cadaver eyes. Wound integrity was evaluated by subjecting each globe to increasing intraocular pressure (IOP), increasing external pressure, and sudden external pressure. Wound leaks occurred in the 7.0-millimeter-incision globes at consistently lower IOPs and with less external pressure than they did in the 5.1-millimeter-incision globes. We conclude that the 5.1-millimeter no-stitch flap incision has greater wound integrity than the 7.0-millimeter sutured incision when subjected to either internal or external pressures.

Cadaver↗
PubMed1994

Cadaver pancreas recovery technique. Impact on islet recovery and in vitro function.

Previous studies in rodent and canine animal models have suggested a detrimental impact on islet recovery and function when pancreas excision is preceded by in situ vascular flushing with cold preservation solutions. We studied the efficacy of islet isolation from 19 consecutive cadaver pancreases procured alternately by initial pancreatectomy before in situ flush (group 1, our standard procurement technique, n = 9) or pancreas removal following in situ vascular flushing with cold University of Wisconsin solution (group 2, n = 10). Once procured, pancreases were weighed, the main pancreatic duct was cannulated, and 150 ml of collagenase solution was injected. The pancreases were transported to the isolation laboratory and processed within 2 hr. Islets were counted and sized after dithizone staining, and the islet equivalents were calculated. Aliquots of isolated islets were cryopreserved using established techniques. Islet function of both freshly isolated and frozen-thawed islets was assessed using a glucose-stimulated perifusion system. Significantly more pancreas was harvested after University of Wisconsin flush (90.6 +/- 6.9 g for group 1 versus 66.7 +/- 4 for group 2, P < 0.05). The quantity of islets per gram of processed pancreas released during enzymatic digestion from each of the experimental groups did not differ significantly (4.5 +/- 0.6 x 10(3) islet equivalents per gram for primary pancreatectomy versus 4.0 +/- 0.4 x 10(3) University of Wisconsin flush). Similarly, following Ficoll purification, the overall yields of islets did not differ significantly. Total islet yield in the primary pancreatectomy group was 181 +/- 25 x 10(3) islet equivalents (2.7 +/- 0.3 x 10(3) IE/g) versus 217 +/- 41 x 10(3) for the University of Wisconsin flush group (2.9 +/- 0.8 x 10(3) islet equivalents/g; P not significant). No differences were observed in in vitro viability. Perifusion stimulation indexes (peak/basal insulin release) were 5.9 +/- 1.3 for group 1 and 7.1 +/- 1.5 for group 2. These results conflict with published results in animal models and indicate that large numbers of viable islets can be recovered from cadaver pancreas utilizing either procurement technique. The decreased operating time, simplicity, and safety favor the use of total pancreatectomy after limited in situ vascular flushing as the method of choice for pancreas procurement for subsequent islet isolation.

Cadaver↗
PubMed1994

Estimation of adipose tissue mass by magnetic resonance imaging: validation against dissection in human cadavers.

The evaluation of adipose tissue distribution has become an essential component of investigations on the complications of obesity. However, a major limitation is lack of methodology for accurate estimation of adipose tissue mass in the different regions of the body. Therefore, we have tested the accuracy and precision of magnetic resonance imaging (MRI) as a method to measure adipose tissue mass in regions of the body not accessible with standard anthropometric methods. The mass of subcutaneous and intraabdominal adipose tissue estimated by MRI was compared with that obtained by direct weighing of the same adipose tissue compartments after dissection in human cadavers. MRI was performed on three unembalmed cadavers (two males, one female) who were subsequently dissected to isolate intraperitoneal, retroperitoneal, and subcutaneous adipose tissues. These same components were delineated by MRI. The results of the two methods were highly congruent. For the various compartments, the mean of the difference between the two methods was only 0.076 kg (95% confidence interval + 0.005 kg and + 0.147 kg). The "limits of agreement" between the two techniques were -0.066 kg and +0.218 kg. Multiple repeated estimates of mass of adipose tissue compartments were made to determine reproducibility of the MRI measurement; the coefficient of variation for repeated measures was below 14%. The results of this study show that MRI is an accurate and precise technique to evaluate adipose tissue mass in subcutaneous and intraabdominal compartments. Furthermore, MRI was found to be a valid method to separately evaluate the mass of intraabdominal subcompartments of intraperitoneal and retroperitoneal adipose tissue.

Abdomen↗
PubMed1976

Long term survival and graft function after cadaver kidney transplantation.

During the period 1968-75 310 cadaver kidney transplantations were carried out; 261 first-time, 45 second-time and 4 third-time transplantations. Graft survival after 5 years was about 30% for both the first and the second transplant. Patient survival after 5 years was about 50%. No relationship could be found between match grade and graft survival whereas the patient survival was definitely better in the younger age groups. The major cause of graft loss was rejection, vascular and ureteral complications being less frequent. The main cause of lethal outcome was complications to the immunosuppressive therapy and to a lesser degree cardiovascular events and malignant disorders. Cadaver kidney transplantation is an acceptable treatment of chronical renal failure particularly in younger persons and in cases where dialysis treatment is difficult.

Adult↗
PubMed1993

[Arterial embalming method of the cadaver and its application to research].

Ever since 1974, the cadaver has been embalmed by the arterial embalming method, using pre-embalming fluid with blood clot disperser and cell conditioner for the removal of blood clots and drainage of blood, at the Department of Anatomy of the Kawasaki Medical School. According to this method, the cadavers are always very well fixed so that they can be used for not only anatomical dissection but also research for the vascular system by vasography, kinematics of the joint and other histologic examinations. In this report we have described our embalming procedure concretely and its application to research.

Angiography↗
PubMedDate not supplied

Keratoprosthesis as an aid to learning surgical techniques on cadaver eyes.

Practice on cadaver eyes can be an essential aid for residents and inexperienced surgeons in learning operative techniques. Cadaver eyes, however, have been of only limited utility for teaching highly complicated procedures because of the obscuring of intraocular structures caused by the rapid post mortem onset of corneal swelling. We have overcome this problem by the use of a silicone keratoprosthesis that provides an excellent view of all intraocular tissues. Our model allows the use of donor eyes unsuitable for transplantation and facilitates the learning of even complicated cataract surgery, and vitrectomy techniques on the human eye. It thus can reduce the rate of complications in operations on patients.

Cadaver↗
PubMed1995

[Histological, magnetic resonance imaging, and discographic findings on cervical disc degeneration in cadaver spines: a comparative study].

A total of 210 cervical intervertebral discs were taken at autopsy from 36 cadavers, and underwent both magnetic resonance imaging (MRI) and discography to compare their diagnostic efficacies for investigating degenerative changes in the cervical spine. The age of the subjects had ranged from 43 to 92 years with an average of 68.1 years. Following the autopsy, MRI and discography were performed on the excised cervical spinal column, and the specimen was then prepared for histological examination. The findings were compared with those of the lumbar spine that had previously been reported by Yasuma et al. on 1238 lumbar discs from 197 cadavers ranging in age from 11 to 92 years. The results were as follows: 1) Low intensity in the T2-weighted MRI was well correlated with histological degeneration in the cervical disc disc. 2) The rate of appearance of the posterior protrusion of the cervical disc on the MRI was in accordance with the degree of histological disc degeneration, but it did not always correspond with histological posterior protrusion. There was a remarkably high incidence for false-positive posterior protrusion on the MRI, which should be kept in mind on reading the MRI. 3) In the comparison of the MRI with the discography, a certain positive correlation was found as for disc degeneration, but not in complete accordance. 4) There was a considerable difference in the patterns of degeneration and in posterior protrusion of the discs between the cervical spine and the lumbar spine. The posterior protrusion in the cervical disc was more likely related to horizontal fissure and hyalinization of the posterior annulus, while posterior protrusion in the lumbar disc was often related to reversed orientation of the bundles and myxomatous degeneration of the posterior annulus. This difference was attributed to the difference in the mechanical properties of the cervical and lumbar spines.

Adult↗
PubMed1996

Contact versus noncontact diode laser transscleral cyclophotocoagulation in cadaver eyes.

BACKGROUND AND OBJECTIVE: The optimal placement and laser energy levels of a new contact probe with a variable focus used for transscleral diode cyclophotocoagulation were compared with the diode laser noncontact technique. MATERIALS AND METHODS: Fresh cadaver eyes were evaluated by a modified posterior Miyake view using videotape, light microscopy, and scanning electron microscopy. RESULTS: The noncontact diode technique at maximum energy (1.2 J) and contact diode applications at similar energy levels failed to produce obvious gross tissue ciliary body change and mild alterations by histology. Consistent gross, histologic, and scanning electron microscopic damage was noted at 3.0 J or more with the contact probe placed at the surgical limbus and defocused 0.5 to 1.5 mm posteriorly. CONCLUSIONS: This study indicates that the Multilase contact diode laser causes transscleral ciliary body destruction in a cadaver eye model and may have potential clinical usefulness.

Cadaver↗
PubMedDate not supplied

[Organization and results of cadaver kidney transplantation from 1969 to 1973 (author's transl)].

France transplant was founded in order to organize rationally the cadaver kidneys transport and transplantation with is omogenous compatibility tests. 2143 hemodialysis patients have been presently (1-9-72) treated in 82 dialysis center in France; 964 of them are in the France Transplant waiting list. According with recent laws, nervous function cessation is synonimous of death. That made possible, by good resuscitation techniques, to maintain a good level of circulation and oxigenation of organs. Family permit is required for this purpose. 17 medical transporttion staffs are at work in 12 France towns and cooperate with 12 typing laboratoires working with the same techniques and reagents. One permanent secretariat in Paris is always telex connected with all staffs and mantains a continuous up to date patients waiting list. 415 cadaver kidneys were transplanted, 255 in the same town and 160 trabsported from a town another. A significative rise in cold ischemia times happened recently because of the increasing number of transported kidneys.

Cadaver↗
PubMed1977

[Studies on the estimation of the postmortem interval. 1. The temperature of cadaver (author's transl)].

The relationship was investigated between the postmortem intervals and the rectal temperatures of 35 persons who had died at known times and had been subjected to medico-legal autopsies after exposed to changed environments. The influence of air temperature, body size and cause of death upon the cooling rate of cadaver has been found to be complex and not to be analyzed quantitatively. It has been concluded that the time of death cannot be estimated merely by means of the temperature reading of cadaver.

Adolescent↗
PubMed1997

[Storage of cadaver kidney by trans-organ oxygen insufflation].

Transorganic oxygen preservation was used in storage of 87 cadaver kidneys. The kidneys were stored for 14 to 56 h (34 +/- 1.95 on the average). After storage, all organs were transplanted to 87 patients with chronic renal insufficiency in the terminal stage. The patients age ranged from 18 to 55 years. All recipients were divided into 3 groups according to the results of the operation. Group 1 (55 patients)-the function of the organ was restored immediately, blood creatinine normalized on the 5th-10th postoperative day; subsidiary hemodialysis was not needed. Group 2 (22 patients)-the function of the transplant was restored 14-21 days after the operation; 2 to 8 sessions of hemodialysis had to be conducted. Group 3 (10 patients)-the transplant did not function due to acute irreversible canalicular necrosis or irreversible rejection. No correlative dependence between restoration of the transplant function and the duration of storage was noted (r = 0.15). Our experience allows us to assert that transorganic oxygen perfusion can ensure long-term safe storage of cadaver kidneys.

Adolescent↗
PubMed1998

The femoral sulcus angle in neonatal cadavers: a macroscopical and ultrasonographical study.

The shape of the femoral sulcus is an important factor in patellofemoral congruence. The aim of the present study was to investigate the femoral sulcus angle using neonatal cadavers through ultrasonographic and macroscopic observations, in order to evaluate the differences between those two methods and also in order to compare them with adult data published previously. Both knees of 20 neonatal cadavers were examined to measure the femoral sulcus angle. The mean cartilaginous femoral sulcus angle was 145.77 degrees on the macroscopic measurements, and 146 degrees in the ultrasonographic examinations. There was no significant difference between neonatal and adult values and between ultrasonographic and macroscopic measurements (P > 0.05). In the most of the knees two condyles were symmetrical (92.5%). In conclusion the cartilaginous femoral sulcus angle is completely formed in the neonatal age group. Internal and external factors during childhood may be responsible for condylar dysplasia.

Cadaver↗
PubMed2004

Dynamic contour tonometry: a comparative study on human cadaver eyes.

OBJECTIVE: To compare intraocular pressure measurements obtained by recently introduced dynamic contour tonometry (DCT), Goldmann applanation tonometry (GAT), pneumatonometry (PTG), and intracameral manometry in human cadaver eyes. METHODS: Sixteen freshly enucleated human cadaver eyes were deepithelialized and dehydrated with dextran. A tube was placed in the anterior chamber and connected to a transducer and to a bottle system filled with balanced salt solution. The pressure in the eye was then altered between 5 mm Hg and 58 mm Hg. Intraocular pressure measurements were obtained with DCT, GAT, and PTG at each manometric pressure reading. RESULTS: On average, DCT values measured 0.58 +/- 0.70 mm Hg higher than real intracameral pressure. The GAT and PGT showed consistently lower values, -4.01 +/- 1.76 mm Hg and -5.09 +/- 2.61 mm Hg, respectively. At all bottle heights, DCT values were significantly closer to the reference pressure than GAT and PTG (P<.001). CONCLUSIONS: Measurement with DCT provides IOP values significantly closer to true manometric levels than either GAT or PTG. Further studies are warranted to determine its reliability in patients and the effect of corneal thickness.

Aged↗
PubMed1990

Absorption of 308-nm excimer laser radiation by balanced salt solution, sodium hyaluronate, and human cadaver eyes.

Absorption of the excimer laser radiations of 193-nm argon fluorine and 308-nm xenon chloride in balanced salt solution, sodium hyaluronate, and human cadaver eyes was measured. The absorption of these materials as considerably different for the two wavelengths; we found that 308-nm light experienced much less absorption than the 193-nm light. The extinction coefficient (k) for 308 nm was k = 0.19/cm for balanced salt solution and k = 0.22/cm for sodium hyaluronate. In contrast to this, the extinction coefficient for 193 nm was k = 140/cm for balanced salt solution and k = 540/cm for sodium hyaluronate. Two 1-day-old human phakic cadaver eyes showed complete absorption with both wavelengths. Using aphakic eyes, incomplete absorption was noted at the posterior pole with 308 nm and complete absorption was noted with 193 nm. The extinction in the anterior part of aphakic eyes (the first 6 mm) was 4.2/cm for 308 nm, meaning that the intensity of the light is reduced by a factor of 10 after traveling the first 5.5 mm. However, we observed that the material in the eye fluoresces, meaning the 308 nm is transformed into other (longer) wavelengths that travel through the total eye with minimal absorption. Conclusions drawn from this experiment are that the use of the 308-nm wavelength may have undesirable side effects, while the use of the 193-nm wavelength should be consistent with ophthalmic use on both the cornea and the lens.

Absorption↗