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Factors affecting preparation of chromosomes for scanning electron microscopy using osmium impregnation.

Osmium impregnation techniques have become useful for imparting conductivity to tissue specimens for SEM, thereby avoiding coating with gold or other metals. Such techniques have been developed to produce aesthetically pleasing images of mammalian (particularly human) chromosomes prepared by standard cytogenetical methods which use methanol-acetic acid fixation. The present study was designed: (1) to examine changes in the appearance of chromosomes as a result of preparation by osmium impregnation techniques; (2) to assess the function and importance of the various stages of chromosome preparation; and (3) to identify the chemical groups responsible for osmium binding. Methanol-acetic acid fixed chromosomes are known to have lost many proteins during fixation, and appear to be flattened down on the substrate. Osmium impregnation swells these flattened chromosomes to a variable extent, but the result is inevitably an artefact, albeit a useful one, and not a true representation of the chromosome in vivo. The size of chromatin fibres, for example, is the consequence of the degree of protein extraction during fixation, the loss of material during pre-treatments (e.g. trypsin), and the amount of osmium uptake during impregnation. Trypsin pre-treatment removes a surface coating of protein from the chromosomes as well as exposing chemical groups which can react with osmium. The principal reactive site appears to be amino groups, which bind glutaraldehyde, which in turn binds thiocarbohydrazide, to which the osmium becomes attached. Pre-treatments other than trypsin can be used to extract chromosomal material and to reveal different aspects of chromosome structure.

Animals↗

Theories of narcissism and trauma.

The more severe the pathology, the more one is likely to be dealing with sexual and aggressive fantasies and transferences based on actual traumas sustained by the child. Psychic trauma interferes with the normal structuring of experience. The psychotherapist needs to help transform traumatic registrations into more or less normal memories and tolerable affects. This organizing and structuring process, which brings with it increasing cohesion of self- and object-representations, corresponds to a crucial 'self-object' function of the therapist. The 'trauma paradigm' of psychopathology, with its implied organizational point of view, explains this structuring action of analytic therapy and bridges general psychoanalytic theory and special theories couched in object-relations language.

Denial, Psychological↗

[Relationship, resistance and insight as depicted in measures concerning form and content of speech - a textual analysis of the course of a female in-patient with eating disorder].

OBJECTIVES: This multi-methodical single case study examines how relationship, resistance and insight, the important therapeutic factors, are conveyed in a therapeutic process. METHODS: For depicting interaction the textual parameters - direct speech, acknowledgement tokens, activity of speech and personal pronouns - of Formal Psycholinguistic Text Analysis (Overbeck et al., 1996) are applied. The activity of the unconscious theme is measured by the Interferenzindex (Argelander, 1984). The link of emotional experiences with words is depicted by the Computerized Referential Activity method (Mergenthaler and Bucci, 1999). The attainment of emotional insight in the Model of the Therapeutic Cycle is perceived via the Emotion-/Abstraction-Patterns (Mergenthaler, 1997). RESULTS: Three phases are discriminated accordingly: Resistance, characterised by defensive intellectualization, symbolic representation on the basis of the secure relationship and emotional insight and detachment. CONCLUSIONS: The validity of the results in this single case study is guaranteed by the multi-methodical strategy.

Awareness↗

[Signs of change in the affective context of dreams and the psychoanalytic process].

The author inquires whether dreams related in psychoanalysis can be indicator of curative change, and if so what affective conditions go to making this possible. To answer this question convincingly, he puts forward a new model of dream generation. It proceeds from the relation between cognitive elements, regulatory affective processes and species of interaction represented in individual dream situations. Such a model requires a new theory of mental representation, affective processes and memory. For precise analysis of change processes, Moser has recourse to a coding system for dream content. Finally he compares change processes in dreams with those in the psychoanalytic situation and suggests hypotheses on the extent to which changes in dreams can be indicators of changes in the psychoanalytic process.

Affect↗

[Development of the affect system].

The authors show that the development of the affect system commences with affects of an exclusively communicative nature. These regulate the relationship between subject and object. On a different plane they also provide information on the feeling of self deriving from the interaction. Affect is seen throughout as a special kind of information. One section of the article is given over to intensity regulation and early affect defenses. The development of cognitive processes leads to the integration of affect systems and cognitive structures. In the pre-conceptual concretistic phase, fantasies change the object relation in such a way as to make unpleasant affects disappear. Only at a later stage do fantasies acquire the capacity to deal with affects. Ultimately, the affect system is grounded on an invariant relationship feeling. On a variety of different levels it displays the features typical of situation theory and the theory of the representational world, thus making it possible to entertain complex object relations. In this process the various planes of the affect system are retained and practised. Finally, the authors discuss the consequences of their remarks for the understanding of psychic disturbances and the therapies brought to bear on them.

Affect↗

Nonrandom segregation during meiosis: the unfairness of females.

Most geneticists assume that chromosome segregation during meiosis is Mendelian (i.e., each allele at each locus is represented equally in the gametes). The great majority of reports that discuss non-Mendelian transmission have focused on systems of gametic selection, such as the mouse t-haplotype and Segregation distorter in Drosophila, or on systems in which post-fertilization selection takes place. Because the segregation of chromosomes in such systems is Mendelian and unequal representation of alleles among offspring is achieved through gamete dysfunction or embryonic death, there is a common perception that true disturbances in the randomness of chromosome segregation are rare and of limited biological significance. In this review we summarize data on nonrandom segregation in a wide variety of genetic systems. Despite apparent differences between some systems, the basic requirements for nonrandom segregation can be deduced from their shared characteristics: i) asymmetrical meiotic division(s); ii) functional asymmetry of the meiotic spindle poles; and iii) functional heterozygosity at a locus that mediates attachment of a chromosome to the spindle. The frequency with which all three of these requirements are fulfilled in natural populations is unknown, but our analyses indicate that nonrandom segregation occurs with sufficient frequency during female meiosis, and in exceptional cases of male meiosis, that it has important biological, clinical, and evolutionary consequences.

Animals↗

Parental representation in young women who attempt suicide.

A reliable and valid Parental Bonding Instrument was administered to young women who had attempted suicide. Results indicated low parental care and high overprotection. These results are consistent with theoretical formulations of suicidal behaviour, and taken with other studies help provide a profile of parental characteristics which may be associated with such behaviour.

Adolescent↗

Recontact with the analyst following termination: a survey of seventy-one cases.

A questionnaire was sent to graduate members of the Denver Psychoanalytic Society asking for their experience with patients making contact with them after the analysis. They reported that within three years after termination two thirds of "successfully analyzed" patients had recontacted them. Seventy-one cases were surveyed. Most contacts were brief and did not seem to be the result of incomplete analysis. Rather, they aimed to satisfy ongoing needs for: the continuing deidealization of the analyst, the reactivation of the self-analytic function, and the restructuring of self- and object representations by reporting developmentally significant accomplishments to the former analyst. The analyst's acknowledgement appears to be an integral part of the restructuring. This is work which either cannot be done before termination or the need for which has not been recognized during the analysis.

Fantasy↗

Somatosensory control of precision grip during unpredictable pulling loads. I. Changes in load force amplitude.

In manipulating 'passive' objects, for which the physical properties are stable and therefore predictable, information essential for the adaptation of the motor output to the properties of the current object is principally based on 'anticipatory parameter control' using sensorimotor memories, i.e., an internal representation of the object's properties based on previous manipulative experiences. Somatosensory afferent signals only intervene intermittently according to an 'event driven' control policy. The present study is the first in a series concerning the control of precision grip when manipulating 'active' objects that exert unpredictable forces which cannot be adequately represented in a sensorimotor memory. Consequently, the manipulation may be more reliant on a moment-to-moment sensory control. Subjects who were prevented from seeing the hand used the precision grip to restrain a manipulandum with two parallel grip surfaces attached to a force motor which produced distally directed (pulling) loads tangential to the finger tips. The trapezoidal load profiles consisted of a loading phase (4 N/s), plateau phase and an unloading phase (4 N/s) returning the load force to zero. Three force amplitudes were delivered in an unpredictable sequence; 1 N, 2 N and 4 N. In addition, trials with higher load rate (32 N/s) at a low amplitude (0.7 N), were superimposed on various background loads. The movement of the manipulandum, the load forces and grip forces (normal to the grip surfaces) were recorded at each finger. The grip force automatically changed with the load force during the loading and unloading phases. However, the grip responses were initiated after a brief delay. The response to the loading phase was characterized by an initial fast force increase termed the 'catch-up' response, which apparently compensated for the response delay--the grip force adequately matched the current load demands by the end of the catch-up response. In ramps with longer lasting loading phases (amplitude greater than or equal to 2 N) the catch-up response was followed by a 'tracking' response, during which the grip force increased in parallel with load force and maintained an approximately constant force ratio that prevented frictional slips. The grip force during the hold phase was linearly related to the load force, with an intercept close to the grip force used prior to the loading. Likewise, the grip force responses evoked by the fast loadings superimposed on existing loads followed the same linear relationship.(ABSTRACT TRUNCATED AT 400 WORDS)

Adult↗

A three-dimensional analysis of a bioprosthetic heart valve.

A three-dimensional finite element model of the leaflets of a bicuspid bioprosthetic heart valve is presented. The model is based on a non-linear elastic representation of the tissue behaviour which closely simulates that found in experiments. The geometry of the model is based on measurements from a real valve. Shell elements which permit bending have been used in the analysis. The results indicate that bending stresses in the leaflets make a significant contribution to their deformation. This confirms earlier two-dimensional work which had suggested that analyses, where only membrane stresses were modelled, were likely to produce significant errors in the stress states. The analysis also predicts peak stresses close to, but not at, the attachment of the leaflet to the stent post.

Biomechanical Phenomena↗

Clinical aspects and strategy for biomaterial engineering of an auricle based on three-dimensional stereolithography.

At the present time, the partial and/or complete reconstruction of an auricle from autologous rib cartilage is one of most widely published techniques. In the field of tissue engineering, different techniques have been described to generate cartilage tissue using isolated chondrocytes. The basis of these tissue-engineering techniques is bioresorbable or non-bioresorbable biomaterials, which serve as a three-dimensional cell carrier. Tissue engineering of an auricle requires preformed bioresorbable biomaterials designed to fit the form of a patient's auricular defect. Three-dimensional imaging acquired from computed tomography scans or laser surface scanning has become an important tool in modern medicine. This study represents the preoperative procedures for the reconstruction of an auricle through tissue engineering in accordance with the clinical aspects. Hyaff 11, a hyaluronic acid derivative, was used as a three-dimensional cell carrier for isolated human nasoseptal chondrocytes. The chondrocytes were amplified in a conventional monolayer culture before the cells were seeded on a hyaluronic non-woven mesh and cultured in vitro for 4 weeks. The chondrogenic potential of human nasal chondrocytes in Hyaff 11 was investigated by confocal laser scanning microscopy, histology (toluidine blue) and immunohistochemistry (collagen type II). Computer-aided design (CAD) and manufacture of an auricle model with stereolithographical methods were used for the prefabrication of a bioresorbable three-dimensional cell carrier designed in the form of a patient's auricular defect. The cell carrier used was Hyaff 11, a fully benzyl-esterified hyaluronic acid derivative. Confocal laser scanning microscopy has shown good cell attachment, a homogenous distribution of amplified chondrocytes and a viability of more than 90%. After 4 weeks in vitro culture the human nasoseptal chondrocytes synthesized new cartilage with the expression of cartilage-specific collagen type II. In order to shape a patient's designed scaffold the auricle model was fitted exactly and symetrically to the contralateral side. Subsequently, the mirror image patient-specific model was used to prepare an identical scaffold model made of a fully benzyl-esterified hyaluronic acid derivative. The bioresorbable scaffold that was produced gave a satisfactory representation of auricle structure. Bioresorbable preformed biomaterials in the form of a patient's auricle defect represent an important prerequisite for the tissue engineering of autologous auricle grafts. Hyaff 11 seems to be a promising material for tissue engineering of cartilage transplants, and the application of this approach will improve conventional reconstructive surgery in the future.

Absorbable Implants↗

A new system to detect native microaggregates of platelets in vivo, with a novel platelet aggregometer employing laser light scattering.

Circulating platelets are susceptible to various stimuli in vessels, and a certain portion of platelets may thereby form native microaggregates (NMAs), which can be visualized by a bother scanning electron microscopy (SEM). We developed an easy method to evaluate the existence and extent of NMAs and the subsequent spontaneous platelet aggregation (SPA) induced by not any agonist but stirring force with a novel platelet aggregometer (PA-200; Kowa, Tsukuba, Japan) employing a particle-counting method based on laser light scattering. In this system, platelet-rich plasma obtained by a delicate centrifugation was infused in a cuvette to replace the content and to avoid stirring shear. An insertion attachment was designed in a coaxial shape to maintain a constant sample volume. Our data indicated that NMAs, tentatively defined as a "small" division by their light scattering (25-400 of the intensity threshold), could be detected by not only a complicated SEM but also by our simple system with the PA-200 in a few minutes. No correlation was found in the comparison of the existence of NMAs and the SPA generation, in aliquot platelets, respectively. These results suggested that NMAs and SPA might be mutually different cluster and have meanings as representations of platelet status.

Blood Platelets↗

Dimensions of family rituals across two generations: relation to adolescent identity.

Family rituals are considered part of a generational process that fosters a sense of identity for individual members and is reflective of the family's shared belief system. The symbolic significance attached to family rituals is considered central to the force of family rituals. Three questions were addressed in the study: (1) Are the dimensions of family rituals viewed similarly across generations?; (2) Is level of ritualization related to adolescent identity?; (3) If there is disagreement about the relative level of ritualization in a family, is there a negative relation to adolescent identity? A total of 77 families with an adolescent member completed the Family Ritual Questionnaire, and the adolescents completed a measure of self-esteem. Results of a factor analysis demonstrated shared representation of family rituals across two generations, with one factor loading on the symbolic qualities of family rituals and the second factor loading on the routine aspects of family rituals. Positive relations were found between adolescent identity and the family's report of symbolic significance and affect associated with family rituals. A negative relation was found between mother-adolescent disagreement about family rituals and adolescent feelings of belonging. Distinguishing between meaning and routine aspects of family rituals is discussed as well as clinical implications.

Adolescent↗

Internal consistency of the object relations and social cognition scales for the Thematic Apperception Test.

We examined 8 data sets to determine whether it is possible to attain acceptable levels of internal consistency (coefficient alpha) reliability for the 4 Object Relations and Social Cognition scales (ORSC; Westen, Lohr, Silk, Kerber, & Goodrich, 1989) for the Thematic Apperception Test (TAT; Murray, 1943) when cards are considered as items in a scale. Number of cards used in the data sets ranged from 4 to 10, and the Spearman-Brown prophecy formula was applied to estimate the number of cards that would be required to attain alpha levels of different magnitudes. The two more structural subscales (Complexity of Representations and Understanding of Social Causality) have somewhat higher consistencies than the two more affective ones (Affect Tone and Capacity for Emotional Investment and Moral Standards). The results suggest that the use of 10 to 12 cards provides internal consistencies of alpha > or = .70 across each of the 4 ORSC scales.

Cognition↗

Spatial selectivity and binaural responses in the inferior colliculus of the great horned owl.

In this study we have investigated the processing of auditory cues for sound localization in the great horned owl (Bubo virginianus). Previous studies have shown that the barn owl, whose ears are asymmetrically oriented in the vertical plane, has a 2-dimensional, topographic representation of auditory space in the external division of the inferior colliculus (ICx). As in the barn owl, the great horned owl's ICx is anatomically distinct and projects to the optic tectum. Neurons in ICx respond over only a small range of azimuths (mean = 32 degrees), and azimuth is topographically mapped. In contrast to the barn owl, the great horned owl has bilaterally symmetrical ears and its receptive fields are not restricted in elevation. The binaural cues available for sound localization were measured both with cochlear microphonic recordings and with a microphone attached to a probe tube in the auditory canal. Interaural time disparity (ITD) varied monotonically with azimuth. Interaural intensity differences (IID) also changed with azimuth, but the largest IIDs were less than 15 dB, and the variation was not monotonic. Neither ITD nor IID varied systematically with changes in the vertical position of a sound source. We used dichotic stimulation to determine the sensitivity of ICx neurons to these binaural cues. Best ITD of ICx units was topographically mapped and strongly correlated with receptive-field azimuth. The width of ITD tuning curves, measured at 50% of the maximum response, averaged 72 microseconds. All ICx neurons responded only to binaural stimulation and had nonmonotonic IID tuning curves. Best IID was weakly, but significantly, correlated with best ITD (r = 0.39, p less than 0.05). The IID tuning curves, however, were broad (mean 50% width = 24 dB), and 67% of the units had best IIDs within 5 dB of 0 dB IID. ITD tuning was sensitive to variations in IID in the direction opposite to that expected for time-intensity trading, but the magnitude of this effect was only 1.5 microseconds/dB IID. We conclude that, in the great horned owl, the spatial selectivity of ICx neurons arises primarily from their ITD tuning. Except for the absence of elevation selectivity and the narrow range of best IIDs, ICx in the great horned owl appears to be organized much the same as in the barn owl.

Acoustic Stimulation↗

Suggestions for a framework for an empirically based classification of personality disorder.

BACKGROUND: The classification of personality disorder is one of the least satisfactory sections of contemporary psychiatric classification. Fundamental problems with current classifications include extensive diagnostic overlap, limited evidence of validity, and poor empirical support. METHODS: Conceptual analysis and the results of empirical studies are used to propose a framework for organizing an empirically based classification. RESULTS: First, personality disorder is a form of mental disorder and, therefore, should be classified as a single diagnostic entity on Axis I along with other mental disorders. A preliminary definition of personality disorder as a tripartite failure involving the self system, kinship relationships, and societal relationships is proposed. The evidence suggests that this definition can be translated into a reliable set of items. Second, the diagnosis of personality disorder should be separated from the assessment of clinically relevant personality traits. Given the consistent evidential support for a dimensional model of personality disorder, it is suggested that personality be coded on a set of trait dimensions selected to provide a systematic representation of the domain of behaviours represented by current diagnostic concepts. Third, given that personality traits are hierarchically organized, it is suggested that an axis for coding personality include basic or lower-order dimensions as the primary level of assessment and a few higher-order patterns to summarize information for some purposes. CONCLUSION: A preliminary list of 16 basic dispositional traits is proposed to describe the more specific components of personality disorder based, in part, on the convergence of evidence across studies: anxiousness, affective lability, callousness, cognitive dysregulation, compulsivity, conduct problems, insecure attachment, intimacy avoidance, narcissism, oppositionality, rejection, restricted expression, social avoidance, stimulus seeking, submissiveness, and suspiciousness. Three higher-order patterns were proposed: emotional dysregulation, dissocial behaviour, and inhibitedness, which may occur independently or in combination.

Diagnosis, Differential↗

Visual spatial illusions: a general explanation.

Representation at the visual receptors of such properties of the object as its size, shape, orientation, and movement undergo considerable variation as the distance, bearing, posture, and motion of the observer, relative to the object, changes. However, despite these gross and frequent deformations of the image, perceived properties remain extraordinarily stable. Such constancy has obvious biological utility; the observer perceives his world according to its fixed physicalfeatures rather than in terms of its variable sensory representation. Constancy of apparent size, shape, orientation, and movement is dependent on information for the distance, bearing, lateral tilt, and movement of the observer, respectively. Thus, as the retinal image shrinks with distance, constancy of apparent size is maintained by five classes of distance, stimuli that operate singly or in various combinations. Likewise, visual orientation and visual stimuli for the orientation of the observer, and movement constancy on visual and nonvisual stimuli for the movement of the observer. Illusion occur when stimuli that normally preserve constancy are operative but with the image of the object not varied. Thus if retinal disparity, convergence-accommodation, projected stimuli, or other distance stimuli are varied with the image not varied, illusions of size occur. Those rsluting from variation of projected stimuli are the. well-known geometrical size illusions and include the Mü llerLyer group. In essentially the same manner, independent manipulation of stimuli for the orientation and the motion of the observer, with the orientation and the motion of the image at the retina not varied, gives rise to illusory orientations and movements of the object. Limited attempts to explain size illusions in terms of the projected stimuli that preserve perceptual constancy are by no means new; Thiéry (51) proposed such a view in the later part of the last century, and in recent times there has been a spate of such proposals including the " misapplied constancy hypothesis" advanced by Gregory (2). However, Gregory's theory is confined largely to geometrical size illusions and invoks only distance scaling given by a limited number of projected stimuli. Furthermore, the Müller-Lyer illusion is seen by him to be a consequence of distance scaling resulting from the converging arrows. There is no recognition of the range of such effects with various attached elements, as shown in Fig3, and no attention is accorded the recently established difference between the illusions with inward and outward-directed elements. The theory of spatial illusions outlined here distinguishes between classes of illusory effect and, in linking each to its particular class of spatial constancy, offers a general and testable explanation. Failure to recognize classes of illusion(and perceptual constancy), such as those of size, orientation, and movement, can be regarded as among the major deficiencies of recent attempts (2, 44) to explain illusory effects. I do not claim that this explanation, which I call the general constancy theory, satisfactorily encómpasses all known illusions, but merely that it is more comprehensive than alternative explantions I conclude that any stimulus which serves to maintain perceptual constancy of a property of an object as the visual representation of that property varies will, when independently manipulated with the retinal image not varied, produce an illusion. This general principle predictS the conditions under which illusory effects will occur and has wide explanatory application.

Age Factors↗

[Psychodynamic therapy approaches in depressive disorders. Pathogenesis models and empirical principles].

Targets for psychodynamic psychotherapy are the core conflictual relational mode and its consequences for mood, social behaviour, and self image. Libido- and conflict-based concepts of the relational mode are increasingly being replaced by the concept of intersubjectivity, which includes also nonconflictual, healthy modes of relations as a basic human mental task. According to recent mother-infant interaction research, the psychotherapeutic effect on the relational mode can be interpreted in terms of procedural learning rather than insight-oriented discourse. The mother-infant paradigm is a particularly suitable research paradigm to elucidate unconscious procedural acquisition of mental representations of dyadic interactional styles. Procedural learning requires a more active corrective style of interacting on the therapist's side, beyond abstinence and control of countertransference. Evaluative studies show that psychodynamic psychotherapy of depression is nearly as effective as cognitive behaviour therapy (CBT) and interpersonal therapy (IPT).

Adult↗