Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “gene technologies”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 55 records · Page 3Linked to original sources
PubMed1981

[Topicalities from gene technology. Protein synthesized by bacteria the better therapeutic alternative (author's transl)].

Compared with conventional methods for obtaining highly complicated natural substances such as peptide hormones and vaccine antibodies from animal or human tissues or cell culture, the synthesis of these therapeutically important substances in bacterial cells, made possible by gene technology, has the decisive advantage that the bacteria synthesize exclusively that protein whose appropriate genetic information has previously been obtained and insinuated into the bacterial cell under accurately controlled physicochemical conditions. Thus, the mmammalian protein produced in bacteria is purer and consequently freer from side-effects than material obtained from ammmalian cells.

Animals↗
PubMed1988

[Gene technology in the diagnosis of viruses and vaccine development].

The development of genetechnological methods since the beginning of the 1970's allowed the molecular cloning of partial or complete viral genomes and the sequencing of their nucleic acids. On this basis, new tools for viral diagnostics are available: molecular probes for hybridization techniques and synthetic peptides or highly purified proteins for the specific detection of antibodies. While the role of synthetic peptides as vaccines seems to be limited, complete viral surface proteins produced by gene technological methods are already used for vaccination in man. The advantages and disadvantages of production in bacteria, in yeast and in higher eukaryotic cells of polypeptides designed as subunit vaccines are discussed. An additional, attractive model is the synthesis of antigens immediately in the host, directed by a recombinant vaccinia virus. Another promising approach is the establishment of potent and safe live vaccines by the introduction of defined mutations or deletions into a viral genome, based on the previous elucidation of the molecular mechanism of attenuation.

Cloning, Molecular↗
PubMed2006

Gene technology based therapies in the brain.

Gene therapy potentially represents one of the most important developments in modern medicine. Gene therapy, especially of cancer, has created exciting and elusive areas of therapeutic research in the past decade. In fact, the first gene therapy performed in a human was not against cancer but was performed to a 14 year old child suffering from adenosine deaminase (ADA) deficiency. In addition to cancer gene therapy there are many other diseases and disorders where gene therapy holds exciting and promising opportunities. These include amongst others gene therapy within the central nervous system and the cardiovascular system. Improvements of the efficiency and safety of gene therapy is the major goal of gene therapy development. After the death of Jesse Gelsinger, the first patient in whom death could be directly linked to the viral vector used for the treatment, ethical doubts were raised about the feasibility of gene therapy in humans. Therefore, the ability to direct gene transfer vectors to specific target cells is also a crucial task to be solved and will be important not only to achieve a therapeutic effect but also to limit potential adverse effects.

Alzheimer Disease↗
PubMed1989

[Gene technology in animal husbandry, new research approaches exemplified by the milk protein gene of cattle].

For investigation of bovine milk protein genes several methods of recombinant DNA techniques are presented. Possible applications of genome research in animal breeding are given including the characterization of structure and function for single genes, gene mapping as well as screening for gene variants in populations. Hence it follows that scientific and practical developments can be expected and will be an influence on future animal production.

Animals↗
PubMed1994

Introduction to gene technology.

The increased use of biotechnology for numerous categories of common products (pharmaceuticals, foods, agricultural chemicals, etc.) has an ever increasing impact on our society. In the medical/pharmaceutical field, biotechnology signifies a drastic change in the approach to drug discovery, research and development, diagnosis, and disease management. The basis of replication, transcription, translation, recombinant DNA technology, and production of altered genes are defined. Examples of biopharmaceuticals, i.e., enzymes or regulators of enzyme activity, hormones or hormone-like growth factors, cytokines, vaccines, monoclonal antibodies, and gene transfer in humans are discussed.

Animals↗
PubMed2001

Design of polymer materials enhancing nucleotide hybridization for anti-gene technology.

Stabilization of nucleotide hybridization is considered important for improving gene therapy using oligonucleotides. We have designed comb-type copolymer consisting of polycation backbone (polylysine) and hydrophilic side chains as a stabilizer for double and triple helical DNAs. The copolymer considerably increased the thermal stability of triple helical structure but did not affect the reversible transition between triple helical and single-stranded DNA. An in vitro electrophoretic mobility shift assay revealed that the copolymer remarkably increased association constants of both Hoogsteen and reverse Hoogsteen-type triple helix formation. Moreover the triple helix-stabilizing efficiency of the copolymer was significantly higher than that of other oligocations like spermine and spermidine. Not only being good DNA triple helix stabilizer, it has also been shown to accelerate DNA strand exchange reactions between double helical DNA and its complementary oligonucleotides. From these, we conclude that this copolymer is capable of either 'stabilizing' or 'activating' DNA hybrids, and may useful for gene targeting employing oligonucleotides.

Animals↗
PubMed2025

Gene regulation technologies for gene and cell therapy.

Gene therapy stands at the forefront of medical innovation, offering unique potential to treat the underlying causes of genetic disorders and broadly enable regenerative medicine. However, unregulated production of therapeutic genes can lead to decreased clinical utility due to various complications. Thus, many technologies for controlled gene expression are under development, including regulated transgenes, modulation of endogenous genes to leverage native biological regulation, mapping and repurposing of transcriptional regulatory networks, and engineered systems that dynamically react to cell state changes. Transformative therapies enabled by advances in tissue-specific promoters, inducible systems, and targeted delivery have already entered clinical testing and demonstrated significantly improved specificity and efficacy. This review highlights next-generation technologies under development to expand the reach of gene therapies by enabling precise modulation of gene expression. These technologies, including epigenome editing, antisense oligonucleotides, RNA editing, transcription factor-mediated reprogramming, and synthetic genetic circuits, have the potential to provide powerful control over cellular functions. Despite these remarkable achievements, challenges remain in optimizing delivery, minimizing off-target effects, and addressing regulatory hurdles. However, the ongoing integration of biological insights with engineering innovations promises to expand the potential for gene therapy, offering hope for treating not only rare genetic disorders but also complex multifactorial diseases.

Humans↗
PubMed2025

Problematic use of sustainability claims in recent scientific literature on crop gene technologies: toward improving practices and communication.

There is growing interest in the role of agricultural genomics, including biotechnology, in enhancing the "sustainability" of food production systems. However, as "sustainability" becomes more frequently linked to the goals of agricultural genomics, a critical question arises: what claims are made about "sustainability" and how is the concept of "sustainability" defined in the scholarly literature on agricultural genomics? Using a structured analysis of the recent scientific literature, this article investigates increasingly frequent claims about "sustainability," including how this term is defined and measured in the field of agricultural genomics. It argues that more transparent definitions and clearer metrics, tied to appropriate scholarly literature, are crucial for improving the coherence, impact, and credibility of research in agricultural genomics.

Crops, Agricultural↗
PubMed1995

[Gene technology to overproduce the enzymes useful as diagnostic reagents].

We cloned the genes encoding the microbial enzymes described below and discussed the possibility as the diagnostic reagents: Creatinase(CR) and sarcosine oxidase(SOX) are useful for a diagnostic measurement of creatinine in combination with creatinase. Bacillus sp. B-0618 produces both CR and SOX enzymes when grown in the presence of an inducer, choline chloride. We cloned these two genes encoding CR and SOX by using recombinant DNA techniques. We found that the SOX-encoding gene is located upstream from the CR-encoding gene. When the CR- and SOX-encoding genes were independently inserted into the pUC-based vector and introduced into Escherichia coli, the transformants produced 15.5-fold more CR and 50-fold more SOX than Bacillus sp. B-0618, respectively, in the absence of the inducer. Although the Bacillus SOX is a flavoprotein, we created an FAD-free SOX by site-directed DNA mutagenesis. The mutant protein no longer expressed the SOX activity. Cholesterol esterase is useful for a diagnostic measurement of total cholesterol in combination with cholesterol oxidase. We cloned a gene encoding the cholesterol esterase enzyme. When the gene was introduced into the non-producing Streptomyces strain, the transformant produced the cholesterol esterase. Phospholipase A2 is a diverse family of enzymes that hydrolyze the sn-2 fatty acyl ester bond of phosphoglycerides producing free fatty acids and lysophospholipids. The enzymes are present in pancreatic juice and in the venoms of snakes and bees, where they serve digestive functions.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed2001

Array of hope for gene technology.

A Washington-based bioinformatics company is developing sophisticated DNA microarrays that should help researchers measure and analyze gene expression faster, more economically, and with greater precision than ever before possible. The FlexJet system, as the microarray product is known, uses inkjet technology to propel microscopic strands of DNA nucleotides onto slides, "printing" arrays of DNA molecules in a process not unlike the manner in which a printer deposits ink onto paper, forming distinct patterns of characters and images. Microarray technology may revolutionize the field of toxicogenomics by helping scientists target new drugs, discover gene function, determine biologic pathways, and better understand diseases such as cancer, cystic fibrosis, and cardiovascular disease at the molecular level.

Computers↗
PubMed1998

Application of mating type gene technology to problems in fungal biology.

In ascomycetes, the single mating type locus (MAT) controls sexual development. This locus is structurally unusual because the two alternate forms ("alleles") are completely dissimilar sequences, encoding different transcription factors, yet they occupy the same chromosomal position. Recently developed procedures allow efficient cloning of MAT genes from a wide array of filamentous ascomycetes, thereby providing MAT-based technology for application to several ongoing issues in fungal biology. This article first outlines the basic nature of MAT genes, then addresses the following topics: efficient cloning of MAT genes; the unusual molecular characteristics of these genes; phylogenetics using MAT; the issues of why some fungi are self-sterile, others self-fertile, and yet others asexual; the long-standing mystery of possible mating type switching in filamentous fungi; and finally the evolutionary origins of pathogenic capability.

Journal Article↗