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PubMedDate not supplied

Bacteriology of maxillary sinusitis in relation to quality of the retained secretion.

The bacteriological findings in 200 patients with acute maxillary sinusitis are reported. It is concluded that the sampling technique--by antral aspiration--is highly significant in the evaluation of the bacteriological background of sinusitis, whereas the anerobic transport of the sample seems to be of less importance. By aspiration the purulent secretion can be properly separated from the non-purulent secretion. Pneumococci, H. influenzae and anaerobic bacteria can be isolated in about 90% of the patients with true sinus empyema, while sterile conditions are rare.

Anaerobiosis↗
PubMed1977

Surveillance of hospital infections: at the bedside or at the bacteriological laboratory?

A prevalence study concerning wound infections in the surgical service at a university hospital was made during a 7-month period. One ward was visited every week during the wound dressing rounds and samples for bacteriologic culture were taken from clinical infections. The prevalence of infection varied between 0 and 21% (median value 8.2%) for surgical wounds and between 0 and 47% (median value 5.3%) for other wounds. Staphylococcus aureus made up 16% of the bacteria isolated from surgical wounds and the Enterobacteriacae 32.6%. For other wounds Staph. aureus and the Enterobacteriacae made up 22.6% each. A comparison of the number of cultures taken by investigators and that taken by the ward showed that the culturing frequency by the ward staff was so high that surveillance of infection by review of bacteriological culture records only, would give an almost equivalent degree of information concerning infection rates. This system is less time consuming and also has the advantage of giving epidemiological information. The drawback with the system is that little or no patient data are given on the requisitions from the ward. This makes it impossible to know from what kind of infections samples are taken.

Cross Infection↗
PubMed1978

Bacteriologic cure of experimental Pseudomonas keratitis.

Two long-term therapy trials with high concentrations of antibiotic were carried out to determine the duration of therapy required to achieve bacteriologic cure of experimental Pseudomonas keratitis in guinea pigs. In the first study, corneas still contained Pseudomonas after 4 days of continual topical therapy with either tobramycin 400 mg/ml, amikacin 250 mg/ml, ticarcillin 400 mg/ml, or carbenicillin 400 mg/ml. In an 11-day trial of topical therapy with tobramycin 20 mg/ml, 34 of 36 corneas grew no Pseudomonas after 6 or more days of therapy. The bacteriologic response to therapy in this model occurred in two phases. About 99.9% or more of the organisms in the cornea were killed in the first 24 hr of therapy. The numbers of bacteria remaining in the cornea declined gradually over the next several days until the corneas were sterile. Optimal antibiotic therapy may include two stages: initial intensive therapy with high concentrations of antibiotic applied frequently to achieve a large rapid decrease in numbers of organisms in the cornea, followed by prolonged, less intensive therapy to eradicate organisms and prevent relapse.

Administration, Topical↗
PubMed1975

[Bacteriological studies during conservative treatment of periapical inflammations].

32 teeth with the diagnosis of periapical osteitis (established by clinical and radiological examinations) were subjected to endodontic treatment (irrigation with hydrogen peroxide), the course of which was followed by bacteriological examinations. The timing of treatment termination depended solely upon clinical evaluation. The examination of smears taken before the last irrigation showed that 26 of the 32 root canals (81%) were germ-free; in 2 cases, an anaerobic infection was still extant; and in 4 cases, an aerobic infection was observed. The high percentage of root canals which were germ-free before the last irrigation testifies to the efficiency of hydrogen peroxide application for disinfecting root canals on the one hand, and demonstrates the good agreement between clinical and bacteriological findings on the other hand. The germs identified belonged mainly to the normal oral flora. Enterococci proved to be particularly resistant. The conservative treatment of periapical osteitis by means of root-canal disinfection with a hydrogen peroxide preparation ans subsequent root-canal filling with a calcium peroxide paste is recommended.

Calcium Hydroxide↗
PubMed1979

Use of quantitative bacteriologic techniques to diagnose catheter-related sepsis.

A case of polymicrobial sepsis occurred in a patient who had a permanent indwelling hyperalimentation catheter. Because it was undesirable to remove the catheter, quantitative bacteriologic techniques were used to determine whether the catheter was the source of sepsis. Blood drawn from a peripheral vein had 25 colonies per milliliter whereas blood drawn through the catheter had more than 10,000 colonies per milliliter. On the basis of these results, the catheter was removed. The catheter tip was found to be infected with the same organisms that were present in the blood. Quantitative bacteriologic techniques may prove useful in diagnosing catheter-related sepsis when it is undesirable to remove the catheter.

Adult↗
PubMed1977

[Bacteriological findings in chronic infantile tonsillitis (author's transl)].

Under general anaesthesia cultures were taken from infantile tonsils prior to T & A. The bacteriological results and the serological classification of hemophilus are questionning literature reports. According to our findings one must consider haemophilus influencae as a resident of the common oral flora. There was no significant difference culturing tonsils with or without repeated antibiotic treatment. Hemophilus influencae combined with beta-hemolytic streptococci is common. Immunological considerations against infantile T & A, based on bacteriological observations are questioned.

Anti-Bacterial Agents↗
PubMed1977

[Combination of methylmethacrylate (acrylic cement) and antibiotics. Bacteriologic and mechanical study].

The authors report the results of a study about the association of acrylic cement with antibiotics. Three cements have been tested: Simplex, CMW, Palacos, with 14 different antibiotics. A series of experiments has been carried out in three phases: a macroscopic study of the polymerisation of the mixture; in vitro bacteriological studies; and mechanical studies (trials of resistance to traction, flexion, shock measuring the modulus of elasticity and hardness). In vivo bacteriological testings have been carried out on 10 patients. It has come out from this study that the polymerisation of cement, usually does not destroy antibiotics which remain active and that the mechanical properties of cement are modified in sundry manners. All additions of tested antibiotics modifie mechanical properties by about 25 p. 100 but some additions seem to be preferable. Improvements must still be made and confirmed by this type of research.

Ampicillin↗
PubMedDate not supplied

[Radiophotographic and simultaneous bacteriological detection in an industrial group].

After carrying out a complex investigation of the workers of a confections factory the authors detected 2 new cases of tuberculosis (0,5 0/00), with the aid of radiophotography and another 2 cases (3,4 per 1000 subjects examined) by bacteriological investigation of the subjects that coughed. These last 2 cases were detected between those that had a normal thoracic aspect on the radiophotographic film alone. The observation of the authors allows to conclude that a bacteriological investigation of the suspects following radiophotography is not sufficient and that this type of investigation should be extended to certain groups of the population that show broncho-pulmonary symptoms. The association of the two methods, in well-defined technical and organisational conditions will allow for a significant increase of the efficiency of systematic examinations for the detection of pulmonary tuberculosis.

Humans↗
PubMed1975

[Bacteriological characteristics of Streptococcus agalactiae. Incidence and conditions of appearance of the pigmentation. Sensitivity to antibiotics].

From a bacteriological study of 137 strains of streptococcus agalactiae, it seems that this species is perfectly defined by bacteriological tests, the most characteristic of which is hydrolysis with hippuric acid. The brown pigment of the strains, the conditions for demonstration of which are described and discussed here, may also be an important characteristic for it is rare in streptococci. The pigment was extracted, its nature and role are discussed. The sensitivity to 12 antibiotics was studied; the beta-lactamines were the most commonly active.

Aerobiosis↗
PubMedDate not supplied

[Survey on tuberculosis treatment in the Sahara. Preliminary bacteriological results].

The preliminary bacteriological results of a survey carried out in the Sahara desert and concerning antituberculosis treatment are reported. Two regimens were compared ; a twelve-months regimen SHE/HE and six months regimen RHEZ/RH. Out of 370 patients admitted in one year, 292 had a positive smear, whereas 40 had not any bacteriological proof. 275 strains were isolated and 205 were tested to antituberculosis drugs; 15% showed a resistance to a least one drug. The 15% rate was in fact very different from one sanitary district to another. The search for INH metabolites in the urines of patients before treatment showed 67 positive cases out of 341. The follow up was carried out at the 5th, 6th, 9th and 12th month after admission, smears and cultures of sputum and detection of INH metabolites in urines were used.

Algeria↗
PubMed1979

The bacteriology of perimandibular space infections.

The bacteriology of 21 perimandibular clised-space infections was studied prospectively using optimal bacteriological techniques to collect, transport, and process specimens. There was an average of six microbial species per specimen, including approximately four anaerobes and two aerobes. The predominant aerobes were alpha-hemolytic and non-hemolytic streptococci; the predominant anaerobes were peptostreptococci, Bacteroides melaninogenicus, and Fusobacterium nucleatum. These findings indicate that most perimandibular space infections involve a polymicrobial flora in which anaerobic bacteria are the dominant isolates. Antimicrobial decisions should account for these observations, particularly in cases where cultural data are not available or when anaerobic cultures are not performed with optimal techniques.

Adult↗
PubMed1979

[H.R.P.-System 2000: a new universally applicable ventilation system for single or limited multiple use with high bacteriologic-hygienical safety (author's transl)].

A new bacteriologically safe system for artificial respiration, H.R.P.-System 2000, is presented. Only two different SETS for artificial respiration of prematures, newborns and infants (P.N.J.) and adults, children and todlers (A.T.) are necessary. They can be adapted very easily on different types of respirators. Besides the tubing and expiratory valve the H.R.P.-SETS include a highly efficient electronically controlled humidifier working on the principle of gas bubbling through a heated water bath, a special tracheal tube adapter with an automatic water exhaust and flexible gas-reservoir for manual ventilation as well as C.P.A.P. or I.M.V. therapy. The H.R.P.-SETS are available as steril disposables. Resterilisation with gas on several occasions is possible. In a control study on thirty children, mainly premature infants and newborns a conventional SET, similar to the Bennett respiration system with disposable tubing and the H.R.P.-SETS are compared under the conditions of our intensive care unit. Using the criteria of serial bacteriological examinations of the tracheo-bronchial secretions and clinical criteria, H.R.P.-SETS proved superior. Artificial respiration without bacterial contamination by hospital strains for more than 10--14 days seems to be possible as a routine in newborns and children.

Adult↗
PubMed1977

Quantitative bacteriology and pathology of the lung in experimental Pseudomonas pneumonia treated with positive end-expiratory pressure (PEEP).

Mechanical ventilation with positive end-expiratory pre-sure (PEEP) is widely used to treat ventilatory failure complicating pulmonary infection. The present experiment was carried out to test the hypothesis that PEEP is beneficial in an experimental model of canine pneumonia studied for 24 hours. Sixteen mongrel dogs were assigned to ventilation with either zero end-expiratory pressure (ZEEP) or 10 cm H2O PEEP. Pneumonia was induced in half of each group by intratracheal inoculation with Pseudomonas. Tissues for quantitative bacteriology and pathology were obtained at the time of death at 24 hours. Three of four infected-ZEEP dogs died before 24 hours. The geometrical mean of quantitative bacterial counts from infected-ZEEP lobes was 2.0 X 10(6) (+/-3.9) (organisms/gm of tissue), while the mean of the infected-PEEP lobes was 1.7 X 10(4) (+/-3.6) (p less than 0.05). Semiquantitative pathology scores indicated greater injury to the ZEEP-infected than to the PEEP-infected lungs. Quantitative bacteriology and microscopic evidence of parenchymal injury were positively correlated. Thus PEEP-treated animals had lower quantitative bacterial counts, less microscopic pulmonary damage, and improved survival. The advantage conferred by PEEP may be due to facilitation of local mechanisms of pulmonary defense against infection, to increased systemic resistance to sepsis, or to both.

Animals↗
PubMed1977

A bacteriological study of some frozen and nonfrozen foods.

Over a period of 19 months, a total of 331 food samples were submitted to the Food Section of the Bacteriology Division for bacteriological examination. These included 184 samples of frozen seafoods from exporters and 147 samples of fresh, nonfrozen foods from food caterers. The total bacterial count for frozen seafoods ranged from 1 x 10(2) to 2.98 x 10(6) per gm with a mean of 2.14 x 10(5) per gm. Coliforms, Escherichia coli and Staphylococcus aureus were present in 48.9%, 3.3% and 8.2% of the samples examined respectively. Two of the cooked prawn samples showed the presence of Vibrio parahaemolyticus. For the fresh, nonfrozen foods, the total bacterial count ranged from 1 x 10(2) to 3.87 x 10(6) per gm with a mean of 2.58 x 10(5) per gm. The examination also showed that 74.8% were coliform positive, 14.9% were E. coli positive, and 4.8% were S. aureus positive. V. parahaemolyticus was not isolated in any of the samples tested. Other pathogens, namely, Vibrio cholerae, Salmonella and Shigella were not isolated from any of the foods examined. The bacterial levels in these foods were determined and their sanitary and public health significance is discussed.

Animals↗
PubMed1979

Quantitative bacteriology in wound care.

Quantitative bacteriology in the management of wound sepsis provides an objective way to monitor the success or failure of wound care. The clinical appearance of a wound requiring skin grafting may be misleading. Grafting wounds that appeared clinically ready was unsuccessful when analysis showed bacteriological counts of 10(5) or greater organisms per gram of tissue. A rapid and simple method for quantitative tissue culture is described. Three cases are described illustrating the value of the results of the method.

Aged↗
PubMed1979

[Bacterial infections in renal homotransplant recipients. Results of regular bacteriological controls (author's transl)].

Bacterial infections were studied in 51 immunosuppression kidney transplant recipients. Eighty infectious episodes occured in the first six post-transplantation months. Forty percent of these episodes were nonfebrile, detected during the routine bacteriological follow-up examination. Out of these 80 bacterial infections, 59 were localized (47 in the urinary tract, nine in the surgical wound and three in the lungs) and 21 had spread. Neutopenia occured in 13 patients, in association with septicaemia in eight of them. Usually septicaemia appeared before neutropenia. In 12 cases, infectious episodes (10 septicaemias and two localized infections) and transplant failure were closely related. The bacterial strains responsible for these infections were essentially: Staphylococcus spp (39), Streptococcus spp (14), Klebsiella pneumoniae (9), Escherichia coli (9). Most of the localized infections cleared up when treated with a specific antibiotic. Seven failures of antibiotic treatment were observed in 21 cases of generalized infections. The importance of regular post-transplantation bacteriological check-up in detecting and treating infectious complications in graft recipients must be emphasized.

Adolescent↗
PubMed1977

[Clinical, bacteriological and histological studies following several years of IUD use for contraception].

It is informed about clinical, bacteriological and histological examinations of 108 women who had used IUD's for 2 years. The examinations were carried out on the IUD and on the endometrium. We made use of the transcervical technic of taking out under sterile conditions. Pathological findings could only be fixed amongst 4 women in clinical view. In bacteriological view numerous germs of quite different kind were found at the same patient. Optional pathological germs as they also exist in the cervix were predominant. Of an infection was only spoken if in correspondence of the total material the same germ was found. This concerned only in 6% of the women. Histologically adequate findings resulted. Genuine inflammations were only considered in 6% of the examinated female patients.

Adult↗
PubMed1979

[Cytological and bacteriological study of the vagina in newborn in a hospital environment (author's transl)].

An attempt has been made to ascertain the percentage of bacteriological transmission in the vaginal cavity, in a series of 98 studied cases between mother and daughter. All these findings have been correlated with the hormone quantities shown in smear tests. As a result of the work carried out it has been proved that transmission does not necessary take place, from the bacteriological point of view, in spite of the fact that E. coli is the bacteria most frequently found in cultures of smears from the vagina of both mothers and their newborn daughters. Similarly it was confirmed that differences between indices and percentages of cells from the vaginal epithelium of mothers and daughters is significant and are influenced by the presence of germs, among other factors.

Age Factors↗