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At least 55 records · Page 3Linked to original sources
PubMedDate not supplied

Some results obtained in the "Stefan S. Nicolau" Institute of Virology by the study of herpes simplex virus.

The results obtained in the "Stefan S. Nicolau" Institute of Virology by the study of herpes simplex virus (HSV) are reviewed. The investigations were mainly focused on HSV biology, genetic markers, experimental HSV infection, correlation between HSV and some diseases, action of some chemical and biological preparations on HSV, in vitro and in vivo HSV oncogenicity, HSV-induced surface antigens, a.o.

Animals↗
PubMedDate not supplied

Results in the study of virus--tumor relationships obtained in the "Stefan S. Nicolau" Institute of Virology.

The results of over 200 scientific papers in the field of in vivo and in vitro virus--tumor relationships, worked out in the "Stefan S. Nicolau" Institute of Virology during a period of over 25 years, are very briefly reviewed. Stress is laid on the original theoretical and practical contributions of the institute to the knowledge of viral carcinogenesis in animals and humans. Some personal viewpoints in the interpretation of results are presented and new research prospects in this field--which represents a traditional concern of the institute--are lutlined.

Animals↗
PubMed1975

Further virological and clinical investigations on the attenuated E5"14" virus from the tick-borne excephalitis complex.

A total of 22 persons, given a single intramuscular dose of 3.1 times 10(6) newborn mouse ic LD50/ml of the E5"14" virus (stored for 26 months at 4 degrees C in lyophilized state), were subjected to rigorous clinical, clinical-laboratory and virological investigations. Clinical observations and laboratory tests (blood and cerebrospinal fluid cytology and biochemistry) revealed no ill-effects or deviations attributable to the immunization procedure. After administration of the immunizing dose, no viraemia was detected and specific seroconversions from negativity to positivity were found after 9 weeks in 73-82% of the subjects, using different tick-borne encephalitis (western subtype) viruses in the virus neutralization experiments. The results obtained seem to give increased confidence in the further use of this attenuated virus clone with strictly defined characteristics.

Adult↗
PubMedDate not supplied

Research on viral hepatitis performed in the "Stefan S. Nicolau" Institute of Virology.

The first phase of research on viral hepatitis (VH) performed in the Institute of Virology (1949--1967) consisted of etiopathogenic, clinico-epidemiological and histopathological investigations which outlined the concepts of VH plurietiology, of infravirus, as well as the characteristic symptomatic triad, the "age group" phenomenon, the clinico-morphological characteristics of sclerogenous hepatitis. The 2nd research phase was mainly devoted to the study of the distribution, properties and clinico-epidemiological correlations of HBsAg. The application of a modern methodology confirmed many of the previously obtained results.

Academies and Institutes↗
PubMedDate not supplied

Study of arboviruses in Romania (1958--1978). Results obtained in the "Stefan S. Nicolau" Institute of Virology.

The results obtained in the Institute of Virology by the study of arboviruses in Romania are reviewed. Isolation of tick-borne encephalitis virus and detection of specific serum antibodies in humans, animals and migratory birds demonstrate its presence on the territory of Romania. Serum antibodies could also be detected to other three flaviviruses (West Nile, Ntaya, dengue types 1, 2, 3), though none of them could be isolated in our country. However, isolation of Kemerovo and Tahyna viruses and detection of antibodies to these viruses as well as to Calovo virus draw attention to the possibility of human infections with arboviruses belonging to Kemerovo, California and Bunyavirus groups.

Animals↗
PubMedDate not supplied

Research on respiratory virus diseases performed in the "Stefan S. Nicolau" Institute of Virology.

The studies on respiratory virus diseases performed for 30 years in the "Stefan S. Nicolau" Institute of Virology approached various aspects of the ecology of inlfuenza and parainfluenza viruses, adenoviruses and other respiratory viruses, including numerous experimental, fundamental, clinical and morphopathological investigations. Special attention was paid to specific influenza prophylaxis and to the preparation of an inactivated influenza vaccine applicable by nasal and oral route.

Animals↗
PubMedDate not supplied

Studies on the infectivity of viral nucleic acids performed in the "Stefan S. Nicolau" Institute of Virology.

The investigations on viral nucleic acids (NA) performed in the Institute of Virology since 1957 refer to the infectivity of NA (RNA or DNA) from large, complex viruses, from RNA viruses (ECHO, MM Coxsackie, etc.), from modified viruses, as well as to the biological activity (oncogenicity and infectivity) of NA from tumors and leukemic tissues of human, murine, bovine and avian origin. Studies are reviewed as regards the action of physical and chemical agents on the behaviour of viral and cellular NA and the implications of the use of such agents in viral vaccine preparation are discussed.

Animals↗
PubMedDate not supplied

Thirty years of research on animal virus diseases performed in the "Stefan S. Nicolau" Institute of Virology.

The results of a 30-year research activity in the field of animal virus diseases performed in the "Stefan S. Nicolau" Institute of Virology are briefly reviewed. Investigations referred to swine pest, fowl pest, Newcastle disease, fowl pox, sheep pox, Aujeszky's disease, Rubarth virus, foot-and-mouth disease, chlamydial infections, etc. Mention is made of the preparations obtained for the specific prophylaxis of most of these diseases.

Animals↗
PubMed1979

[Virological diagnosis of epidemic keratoconjunctivitis (author's transl)].

Among 180 patients with proved adenovirus infection of the eye, 144 infections with adenovirus 8 (Ad 8) were found. 11 or 7 patients were infected with Ad 19 or Ad 4 respectively; further 18 cases were associated with 6 other serotypes. The main symptoms of epidemic keratoconjunctivitis (EKC) (pronounced inflammatory swelling of the plica and caruncula, nummular corneal infiltations) are not confined to Ad 8 infections. For the virological diagnosis, a combination of virus isolation and serology is recommended. Virus isolation is mostly successful from conjunctival swabs obtained during the first week of the disease. For serological investigation, the first blood specimen should be taken as early as possible, the second not earlier than 15 days after onset of disease. The mere application of the group-specific adenovirus complement-fixation is insufficient; it should be supplied by neutralization and hemagglutination-inhibition with Ad 8 (or Ad 19) virus, although these reactions are not invariably type-specific. A swifter diagnosis of the adenovirus infection by immunofluorescence performed in cell cultures inoculated with conjunctival material, 3 or 7 days after inoculation, was often successful; however, this procedure proved to be less sensitive than virus isolation.

Adenoviridae Infections↗
PubMed1979

Epidemic dengue hemorrhagic fever in rural Indonesia. I. Virological and epidemiological studies.

Virological studies were carried out during an epidemic of dengue hemorrhagic fever in Central Java, Indonesia in 1976. Dengue virus was isolated from the acute sera of 45 of 69 patients (65%). The isolation rate was higher in primary than secondary cases. Dengue 3 was the predominant serotype being transmitted (27 isolates), but both dengue 1 (8 isolates) and dengue 4 (10 isolates) were also being transmitted. A composite picture of magnitude and duration of viremia showed that many patients were circulating over 10(8) MID50 per milliliter dengue 3 virus for the first 3 days of illness and that viremia persisted for 5-6 days in some persons. If all shock cases were considered, there was no relationship between dengue serotype and severity of disease. All three confirmed fatal cases, however, were associated with dengue type 3 infections.

Antibodies, Viral↗
PubMed1977

Virological and immunological characteristics of tumors induced in adult rats by Rous sarcoma.

Forty primary tumors of Wistar rats and first seven passages of tumors of August rats were investigated by different virological and immunological methods. The tumors were induced in adult rats by Schmidt--Ruppin strain of Rous sarcoma. Based on the virus-cell interaction, the tumors were divided into four groups. The first group of tumors of Wistar rats (2 out of 40) contained mature RSV and viral antigens could be detected by means of the FA test, while the rats had virus neutralizing antibodies in their sera. The second group of Wistar rat tumors (19 out of 40) and August rats tumors contained virus connected with the cells. The third group was composed of "virus-free" tumors of Wistar rats (8 out of 40). Application of the method of cells association increased rescue RSV in tumors of the 2nd group by 2--4 fold and caused activation of the virus in tumors of the 3rd group. Irradiation of rat tumor cells with a dose of 5000-10 000 R did not enhance the rescue or activation of oncogenic virus in any case. In tumors of the second and third group viral antigen was detected by the FA test in 47% and gs antigen by the CF test in 17%. gs antibodies were found in 29% of the sera from rats with these tumors. The 4th group of Wistar rats tumors were "virus-free" (11 out of 40) showing a negative effect of RSV activation. These tumors with one exception contained no virus antigen, and there were no antibodies against them in sera of the rats.

Animals↗
PubMedDate not supplied

[Virological and serological study in the herpetic gengivostomatitis (author's transl)].

Virological and serological investigations to verify herpetic etiology have been performed on 24 children and 1 adult with acute gengivostomatitis. Viral isolation and detection of antibodies against HSV, carried out by neutralization, complement fixation, and indirect immunofluorescence techniques, have confirmed this etiology in the 88% of cases.

Antibody Formation↗
PubMed1978

Classification of rotaviruses: report from the World Health Organization/Food and Agriculture Organization Comparative Virology Program.

The Reoviridae working team was established under the World Health Organization/Food and Agriculture Organization Comparative Virology Program in 1975. The generic name rotavirus has been adopted for the reovirus-like agents associated with diarrhea in man and animals, and the Nebraska calf diarrheal virus strain of bovine rotavirus has been selected as a candidate reference virus. Stocks of this virus and of gnotobiotic calf antiserum have been prepared. Antigenic differences among rotaviruses isolated from different species were recognized on the basis of virus-neutralization tests; a possible association between antigen specificity and variation in the RNA segments and structural proteins of rotaviruses was noticed.

Antigens, Viral↗
PubMed1975

[Virological control of laboratory mice].

Virological control of laboratory mice can be obtained by serology or virus isolation from the animals. Advantages and inconveniencies of these two techniques are discussed. Special techniques i.e. preparation of immune sera or cultures of infected tissues, are also described.

Animals↗
PubMed2025

An Evolutionary Framework Exploiting Virologs and Their Host Origins to Inform Poxvirus Protein Functions.

Poxviruses represent evolutionary successful infectious agents. As a family, poxviruses can infect a wide variety of species including humans, fish, and insects. While many other viruses are species-specific, an individual poxvirus species is often capable of infecting diverse hosts and cell types. For example, the prototypical poxvirus, vaccinia, is well known to infect numerous human cell types but can also infect cells from divergent hosts like frog neurons. Notably, poxvirus infections result in both detrimental human and animal diseases. The most infamous disease linked to a poxvirus is smallpox caused by variola virus. Poxviruses are large double-stranded DNA viruses, which uniquely replicate in the cytoplasm of cells. The model poxvirus genome encodes ~200 nonoverlapping protein-coding open reading frames (ORFs). Poxvirus gene products impact various biological processes like the production of virus particles, the host range of infectivity, and disease pathogenesis. In addition, poxviruses and their gene products have biomedical application with several species commonly engineered for use as vaccines and oncolytic virotherapy. Nevertheless, we still have an incomplete understanding of the functions associated with many poxvirus genes. In this chapter, we outline evolutionary insights that can complement ongoing studies of poxvirus gene functions and biology, which may serve to elucidate new molecular activities linked to this biomedically relevant class of viruses.

Animals↗