Study on the ion-permeability of human dental enamel with special reference to an electrochemical approach.
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This was measured qualitatively by using dyes and quantitatively by hydraulic conductance in dentine discs and crown segments in vitro. Both types of preparation demonstrated large regional differences in permeability, with the highest values at the periphery and the lowest in the centre of the disc or crown. As dentine permeability may vary 3-10-fold across a few millimetres, investigators should use as large a surface area as possible to compensate for these regional differences.
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The filtration and diffusion of tritiated water through dentin disks were measured ina split-chamber diffusion cell. The dentin had been cut with a diamond disk and the surfaces modified with a carbide fissure bur or diamond bur. Disks were given a secondary burnishing treatment with a blank bur or a modified blank bur. Burnishing reduced the permeability of dentin cut with a fissure bur.
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The exposure of bovine enamel to an albumin-containing demineralizing solution results in penetration of protein into the porous enamel. Washing of this albumin-containing enamel results in a complete (low pretreatment albumin concentrations) or partial removal of the albumin (pretreatment concentrations greater than or equal to 200 micrograms/ml-1). Subsequent exposure to increasing salt concentrations of fluoride, phosphate, calcium or chloride shows a partial removal of albumin at fluoride or phosphate concentrations of 75 mM while complete removal occurred at 150-200 mM fluoride or phosphate. Exposure to either calcium or chloride, even at 3 M concentrations, showed a negligible albumin release. It is proposed that protein removed at high fluoride or phosphate concentrations is bound by a strong interaction between protein-carboxyl groups and calcium on the surface of the enamel mineral. The partial removal of albumin released at low fluoride or phosphate concentrations indicates an enamel-albumin interaction by means of Ca-bridging between protein-carboxyl groups and mineral phosphates. Finally, it is suggested that salt-free washing removes albumin that has lost its native form upon binding to the partially dissolved crystallites of the enamel. It is concluded that enamel is mainly protected from demineralization by the inhibitory effects of protein penetrated into the pores, in addition to possible protection by the pellicle on the surface.
In 14 dogs aged 6 months with severe fluorosis, the authors studied the effects of intake of calcium glycerophosphate and local remodent application on 45Ca enamel incorporation and the depth of its penetration into the dental hard tissues. The treatment led to a substantial decrease in both enamel 45Ca accumulation and the depth of its penetration under severe fluorosis. Combined use of the drugs was more beneficial in terms of enamel 45Ca permeability.
OBJECTIVES: In order to use cryopreserved teeth in laboratory studies, cryopreservation must not modify the functional properties of teeth, in particular dentin permeability. The purpose of this study was to evaluate the effects of tooth cryopreservation on human dentin permeability. METHODS: Three hundred and sixty freshly extracted human noncarious third molars were used. The teeth were randomly assigned to two groups: 180 teeth were stored in phosphate-buffered saline at 4 degrees C and tested within 4 h of collection; 180 teeth were cryopreserved. Hydraulic conductance was calculated according to the method developed by Pashley (Outhwaite et al., 1976). RESULTS: Cryopreserved teeth presented a hydraulic conductance of 8.97 x 10(-3) +/- 4.21 x 10(-3) microL.cm-2.min-1.cm.H(2)0(-1) slightly higher than the hydraulic conductance of the freshly extracted teeth of 8.03 x 10(-3) +/- 3.87 x 10(-3) microL.cm-2.min-1.cm.H(2)0(-1). No statistically significant difference was found between the two groups. SIGNIFICANCE: Under the conditions of this study, cryopreservation did not modify dentin permeability.
The principles, indications, and techniques of resinifying therapy and the composition of a resinifying agent are described in this article. The permeability and bacteriostasis of the agent and the volume change of the agent after treatment were observed, and 14C-labeled phenoaldehyde showed that the agent not only filled the main canals and accessory canals but also infiltrated into dentinal tubules with a depth one fourth to one third the total length of the tubules. The antimicrobial activity of the agent after polymerization for 2 to 6 months was determined. Even 9 months after treatment, no change in volume of the agent was found in vivo, and sealing of canals was maintained. Seventy-eight teeth in 69 patients with pulpoperiapical diseases were subjected to resinifying therapy and followed up for 6 to 12 months; the success rate was 82.05%. The histologic study indicated that, of the specimens experimentally treated for 6 to 12 months, 85.7% showed mild or no inflammation while 53.6% showed complete healing or an obvious healing tendency. Furthermore, the mechanisms and advantages of this therapy are discussed.
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A theoretical model was devised that describes the dependence of the hydraulic conductance of dentine disks on the decrease in dentine tubule radius and dentine tubule density (number per area of cross-section) in the pulp-enamel direction. A parameter that describes the decrease in tubule radius was calculated from experimental results from the literature.
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