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At least 55 records · Page 3Linked to original sources
PubMed1998

Adhesion formation of the parietal and visceral peritoneum: an explanation for the controversy on the use of autologous and alloplastic barriers?

OBJECTIVE: To compare formation of adhesions after injury to both parietal and visceral peritoneum and to determine the benefit of autologous transplants and alloplastic barriers in adhesion prevention. DESIGN: Experimental prospective animal study and prospective randomized clinical study. SETTING: An academic research environment. PATIENT(S): Forty women undergoing laparoscopy for endometriosis treatment. INTERVENTION(S): In 60 rats, either the visceral or parietal peritoneum was injured and covered with autologous peritoneal transplants in half of the animals. The formation of adhesions was assessed 21 days postoperatively. In women, adhesions were evaluated 3 months after surgery with or without placement of alloplastic barriers on visceral lesions. MAIN OUTCOME MEASURE(S): Adhesions evaluated according to a scoring system. RESULT(S): Adhesions after injury of the visceral peritoneum in rats were significantly more severe than those from the parietal peritoneum. Autologous peritoneal transplants led to fewer adhesions especially after serosal injuries. Female volunteers treated with alloplastic barrier material showed less severe adhesions than the control group. CONCLUSION(S): The present data indicate that the potential to form adhesions is significantly higher in visceral than in parietal peritoneal lesions. The development of adhesions after injury to the visceral peritoneum could be reduced by a synthetic barrier material.

Animals↗
PubMed1996

Fibrinolysis in human peritoneum during operation.

BACKGROUND: A reduced local fibrinolysis seems to be an important mechanism in the formation of adhesions. Peritonitis may cause adhesions, and the aim of the present study was to determine peritoneal fibrinolytic capacity in inflamed and normal peritoneum. METHODS: Biopsy specimens from normal and inflamed human peritoneum were taken at the beginning and end of operation. After extraction plasminogen activator activity (PAA) was determined by using a chromogenic substrate assay in the presence or absence of inhibitory antibodies against tissue-type plasminogen activator (t-PA), urokinase or plasminogen activator inhibitor-1. RESULTS: t-PA exerted 95% of the PAA. PAA was significantly reduced (p < 0.01) during peritonitis (3.0 IU/micrograms protein; range, 0.3 to 4.2) compared with normal peritoneum (7.1 IU/micrograms protein; range, 0.6 to 18.1). A significant reduction (p < 0.05) in PAA occurred during operation both in normal peritoneum (3.8 IU/micrograms protein; range, 0.8 to 8.6) and in peritonitis (0.6 IU/micrograms protein; range, 0.16 to 2.1). Values are given as medians. CONCLUSIONS: The main PAA in human peritoneum was t-PA. The activity was decreasing during operation and reduced in peritonitis. This reduction in PAA might be a local response to inflammation.

Abdomen↗
PubMed2001

Nerve fibers and histopathology of endometriosis-harboring peritoneum.

STUDY OBJECTIVE: To evaluate the presence of nerve fibers and histopathology of normal peritoneum and endometriosis-harboring peritoneum. DESIGN: Prospective, nonrandomized study (Canadian Task Force classification II-1). SETTING: University hospital. MATERIALS: Peritoneal specimen from 40 women with laparoscopic findings of endometriosis (24 confirmed histopathologically, group H, 16 diagnosed by laparoscopy, group L) and from 9 women with no endometriosis (controls). INTERVENTION: Histopathologic examination of peritoneal specimens with nerve fibers identified by immunocytochemistry staining with an antibody to neurofilament. MEASUREMENTS AND MAIN RESULTS: No differences in mean nerve score were seen among the three groups. Degrees of lymphocytic infiltration and mesothelial hyperplasia were higher in group H than in the other two groups (p <0.01 and <0.05, respectively). The degree of lymphocytic infiltration was significantly higher in group L than in the control group (p <0.05). There were no differences in all measurements between women in group H who experienced chronic pelvic pain and those who did not. CONCLUSION: The presence of nerve fibers in peritoneum is not related to endometriosis. Endometriosis-harboring peritoneum contains more lymphocytic infiltration than normal peritoneum. (J Am Assoc Gynecol Laparosc 8(1):95-98, 2001)

Adult↗
PubMed2003

Nonclosure of peritoneum: a reappraisal.

In our specialty, the practice of closing or not closing the peritoneum is still being debated. Our purpose was to review the literature on the subject, to evaluate the advantages and disadvantages of the procedure, and to provide clinical opinions. Closure of the peritoneum either parietal or visceral peritoneum is unnecessary, it is associated with a slightly longer operating time and more postoperative pain, and there are some suggestions that it might cause more adhesion formation. There are more advantages than disadvantages to not closing the peritoneum. We encourage clinicians not to close both parietal and visceral peritoneum.

Animals↗
PubMed2006

A randomised controlled study of non-closure of peritoneum at caesarean section in a Nigerian population.

A prospective randomised study of 54 patients undergoing caesarean section was carried out to determine if non-closure of peritoneum at lower segment caesarean section has advantages over suture peritonisation with regard to postoperative morbidity. A total of 27 women were randomised to closure and 27 to non-closure of peritoneum. Management decisions were made without reference to treatment groups. Statistical analysis compared the outcome between the two groups. The results revealed that anaesthesia and operating times were significantly shorter in patients that had the peritoneum left open. The incidence of febrile morbidity, wound infection, duration of postoperative and duration of hospital stay were not significantly different in both groups ( p < 0.05). The study concludes that non-closure of peritoneum is associated with significantly reduced operation and anaesthesia time with a non-significant difference in immediate postoperative outcome. Non-closure of peritoneum should be adopted at caesarean delivery.

Adult↗
PubMed1998

An essential role for tumor necrosis factor in natural killer cell-mediated tumor rejection in the peritoneum.

Natural killer (NK) cells are thought to provide the first line of defence against tumors, particularly major histocompatibility complex (MHC) class I- variants. We have confirmed in C57BL/6 (B6) mice lacking perforin that peritoneal growth of MHC class I- RMA-S tumor cells in unprimed mice is controlled by perforin-dependent cytotoxicity mediated by CD3(-) NK1.1(+) cells. Furthermore, we demonstrate that B6 mice lacking tumor necrosis factor (TNF) are also significantly defective in their rejection of RMA-S, despite the fact that RMA-S is insensitive to TNF in vitro and that spleen NK cells from B6 and TNF-deficient mice are equally lytic towards RMA-S. NK cell recruitment into the peritoneum was abrogated in TNF-deficient mice challenged with RMA-S or RM-1, a B6 MHC class I- prostate carcinoma, compared with B6 or perforin-deficient mice. The reduced NK cell migration to the peritoneum of TNF-deficient mice correlated with the defective NK cell response to tumor in these mice. By contrast, a lack of TNF did not affect peptide-specific cytotoxic T lymphocyte-mediated rejection of tumor from the peritoneum of preimmunized mice. Overall, these data show that NK cells delivering perforin are the major effectors of class I- tumor rejection in the peritoneum, and that TNF is specifically critical for their recruitment to the peritoneum.

Animals↗
PubMed2000

CA125 production by the peritoneum: in-vitro and in-vivo studies.

The source of CA125 synthesis is still debated. Endometrial, peritoneal, ovarian and amniotic cells have been demonstrated to produce and secrete CA125. Different studies show that the peritoneum is a source of CA125. The present study aimed at investigating in vivo and in vitro the peritoneal contribution to circulating CA125. Cultures of uterine peritoneum, abdominal peritoneum and myometrium explants were performed and CA125 measured in the culture medium. To modulate the potential production of CA125, the explants were cultured with or without cycloheximide, bacterial lipopolysaccharide (LPS) or ascitic fluid. In a prospective study, we compared a group of patients after abdominal surgery (n = 19; nine men, 10 women) with a group after extra-abdominal surgery (n = 21; 11 men, 10 women), in order to detect a postoperative increase of serum CA125. De-novo synthesis of CA125 could not be demonstrated in the cultures of uterine and abdominal peritoneum and in myometrium, but CA125 concentrations were detectable in the culture medium without being modulated by cycloheximide, LPS or ascitic fluid. After peritoneal surgery, the proportion of patients with increased serum CA125 was significantly higher (P < 0.03) after abdominal surgery as compared with extra-abdominal surgery. This is considered as indirect evidence for in-vivo production of CA125 by the peritoneum.

Abdomen↗
PubMed1994

Immunohistochemical analysis of the peritoneum adjacent to endometriotic lesions using antibodies for Ber-EP4 antigen, estrogen receptors, and progesterone receptors: implication of peritoneal metaplasia in the pathogenesis of endometriosis.

This study was designed to investigate whether or not the pelvic peritoneum exhibits a metaplastic process into müllerian-type epithelium using a marker for epithelial differentiation (Ber-EP4 antigen) and markers that indicate müllerian differentiation (estrogen receptors and progesterone receptors). The peritoneum and/or ovarian surface epithelium adjacent to endometriotic lesions were obtained from 24 patients with endometriosis at operation, and peritoneum and ovarian surface epithelium without any lesions were also obtained from control patients without endometriosis. The specimens were immunohistochemically analyzed using antibodies for epithelial antigen Ber-EP4, estrogen receptor (ER), and progesterone receptor (PR) on frozen sections. Normal peritoneal mesothelium showed negative staining for Ber-EP4, ER, and PR. The mesothelium of the peritoneum adjacent to the endometriotic lesions showed focal positivity for Ber-EP4, ER, and PR. Several cases of ovarian surface epithelium from normal control ovaries and ovaries adjacent to endometriotic lesions also showed focal positivity for Ber-EP4, ER, and PR. Stromal cells accompanying these foci were sporadically positive for ER and/or PR but negative for Ber-EP4. Focal expression of Ber-EP4, ER, and PR in the mesothelium of the peritoneum and the ovarian surface epithelium adjacent to endometriotic lesions suggests that mesothelium possibly acquires characteristics of epithelial as well as müllerian-type nature. These results support an existence of a metaplastic process of the peritoneal mesothelium in the pathogenesis of endometriosis. The more frequent Ber-EP4 positivity in normal ovarian surface epithelium compared to normal peritoneal mesothelium also suggests a fundamental difference in these tissues that may be related to the greater prevalence of epithelial neoplasms arising in ovarian tissue.

Adult↗
PubMed1998

Aquaporin-1 and endothelial nitric oxide synthase expression in capillary endothelia of human peritoneum.

Water transport during peritoneal dialysis (PD) requires ultrasmall pores in the capillary endothelium of the peritoneum and is impaired in the case of peritoneal inflammation. The water channel aquaporin (AQP)-1 has been proposed to be the ultrasmall pore in animal models. To substantiate the role of AQP-1 in the human peritoneum, we investigated the expression of AQP-1, AQP-2, and endothelial nitric oxide synthase (eNOS) in 19 peritoneal samples from normal subjects (n = 5), uremic patients treated by hemodialysis (n = 7) or PD (n = 4), and nonuremic patients (n = 3), using Western blotting and immunostaining. AQP-1 is very specifically located in capillary and venule endothelium but not in small-size arteries. In contrast, eNOS is located in all types of endothelia. Immunoblot for AQP-1 in human peritoneum reveals a 28-kDa band (unglycosylated AQP-1) and diffuse bands of 35-50 kDa (glycosylated AQP-1). Although AQP-1 expression is remarkably stable in all samples whatever their origin, eNOS (135 kDa) is upregulated in the three patients with ascites and/or peritonitis (1 PD and 2 nonuremic patients). AQP-2, regulated by vasopressin, is not expressed at the protein level in human peritoneum. This study 1) supports AQP-1 as the molecular counterpart of the ultrasmall pore in the human peritoneum and 2) demonstrates that AQP-1 and eNOS are regulated independently of each other in clinical conditions characterized by peritoneal inflammation.

Adolescent↗
PubMed1994

Osmotic barrier of the parietal peritoneum.

Fluid movement into the peritoneal cavity results after instillation of a hypertonic solution. Some investigators have assumed that the peritoneum is a significant barrier to small solutes and have predicted that fluid would be drawn by an osmotic gradient into the cavity from the tissue surrounding the peritoneal cavity, resulting in tissue hydrostatic pressures well below atmospheric pressure. Contrary to this, we have previously shown that protein and fluid cross the peritoneum and enter the tissue at the same rate during either isotonic or hypertonic dialysis. To investigate the nature of the osmotic barrier of the peritoneum, the hydrostatic pressure profiles were measured in the abdominal wall of the rat during conditions of either isotonicity or hypertonicity in the peritoneal cavity and constant intraperitoneal hydrostatic pressure (Pip). Measurements were made with a micropipette mounted on a micromanipulator and connected to a servo-null pressure measurement system. No interstitial pressures below atmospheric pressure were observed with either type of solution in the peritoneal cavity. For the three Pip values tested, there were few significant differences between the corresponding pressure profiles of isotonic or hypertonic solutions. It is concluded that the parietal peritoneum is not a functional barrier to small solutes, which are often used to raise the osmolality of intraperitoneal solutions. This finding also implies that the tissue interstitium underlying the parietal peritoneum is not the source of water flow into the cavity, which is observed during hypertonic dialysis.

Abdominal Muscles↗
PubMed2005

Hypertonic glucose solution 10%-25% on the mesenterium and peritoneum of the rat: macroscopic and microscopic study.

PURPOSE: The objective of the experimental study is to detect the macroscopic and microscopic alterations of the mesenterium and parietal peritoneum when hypertonic glucose aqueous solution 10%-25% is administrated into the peritoneal cavity of the rat. METHODS: 90 Wistar females young rats adults were used weighing between 180-250 g, numbered 1 to 90, establishing unique group and divided in three groups (A, B, C) of 30 animals chosen aleatory manner. 0.9% saline solution was used called control group, or group A, 10% glucose solution named group B, and in the others 30 was used 25% glucose solution named group C, differing in the observation period, (06 h, 24 h and 48 h), but with the same procedure. A midline abdominal wall laparotomy was made and in the animals of the control group was injected 2 ml of a 0.9% saline solution into the peritoneal cavity. After, we made a suture in mass without to include the peritoneum. For the others groups (B, C) the rats received 10% glucose solution and 25% glucose solution injected into the peritoneal cavity respectively. All groups were kept under observation and the results were submitted to statistical analysis by a longitudinal and transversal comparative study. RESULTS: A new surgery was done in 6 h, 24 h and 48 h, and we observed in macroscopic evaluation, the presence of fluid, serous uniform and rosy all over the cavity. Vascular congestion was present. We dried out 90 fragments of mesenterium and 90 fragments of parietal peritoneum bilateral. In the microscopic study, necrosis was not present. For the mesenterium histological study we observed 16 cases (17.8%) unspecific chronic inflammation, 30 cases (33.4%) hyperplasc linfonod, 10 cases (11.1%) high vascular congestion, 6 cases (6.6%) reaction fibrosis and 28 cases (31.1%) no alteration. For the parietal peritoneum histological study we observed 6 cases (3.3%) reaction fibrosis and 174 cases (96.7%) no alteration. Giant cell was not present. In the statistical analysis statistic there is no significance between the groups (p>0.05). CONCLUSION: Hypertonic glucose solution and NaCl 0.9% on the mesenterium and parietal peritoneum do not produce tissue necrosis in a rat and the inflammation process has the same intensity.

Animals↗
PubMed2001

Improving contact area between the peritoneum and intraperitoneal therapeutic solutions.

A general assumption in peritoneal dialysis or intraperitoneal chemotherapy has been that a volume of 2 to 3 L in the human is sufficient to make contact with the entire anatomic peritoneum. On the basis of our previous experimental work and that of others, it was hypothesized that only a fraction of the anatomic peritoneum was in contact with the therapeutic solution in the cavity over a short period of time. It was also hypothesized that use of agitation of the experimental animal or a surfactant in the dialysis fluid would increase the contact area of the intraperitoneal solution. These hypotheses were tested by developing a method to measure the peritoneal contact area simultaneously with the anatomic peritoneal area. Anesthetized mice (25 to 35 g) received an injection of a relatively large volume (10 ml) of isotonic solution containing a radiolabeled protein that adhered to the peritoneum with which it came in contact. After a dwell of 1 to 24 h, the animal was killed and frozen. Cross sections of the abdominal and pelvic cavities were cut and placed against film to develop into autoradiograms, which represent the linear dimension of fluid contact in each sampling plane. The tissue sections that corresponded to the autoradiograms were stained to display the linear dimension of the anatomic peritoneum in the sampling plane. By imaging both the autoradiogram and the corresponding histologic slide, an estimate of the ratio of the contact area to anatomic area in each plane can be calculated (R(mean) = average of all ratios). Applying this method to mice that were dialyzed with an isotonic salt solution under quiescent conditions for 1 h produced R(mean) = 0.43 +/- 0.03. With rapid shaking of the animal, R(mean) = 0.54 +/- 0.03 (P: < 0.05). Addition of the surfactant dioctyl sodium sulfosuccinate (DSS) 0.5% to the solution under quiescent conditions increased R(mean) to 1.07 +/- 0.03 (P: < 0.001). Lengthening the dwell of the isotonic solution to 24 h increased R(mean) to >0.90. In further study of the effect of the concentration of DSS on contact area, there was a direct correlation of R(mean) with concentrations ranging from 0.0005 to 0.05% DSS. It is concluded that less than half of the mouse peritoneum is in contact with a large volume of solution in the peritoneal cavity. Maneuvers such as agitation and use of surfactant in the intraperitoneal solution increase the fraction of contact area. Also demonstrated was a direct dose-response of contact area versus intraperitoneal concentration of DSS, which may be useful in intraperitoneal therapies of peritoneal dialysis or intraperitoneal chemotherapy.

Animals↗
PubMed2003

[Non-closure of the peritoneum after hysterectomy--postoperative clinical assessment].

Dynamic development in modern medicine permanently brings the modifications of operation techniques. Both closure and non-closure of the peritoneum after gynecologic operations has been discussed recently. According to clinical observations provided by many authors, the controversial problem of non-closure of the peritoneum is still under discussion. Thus we decided to assess the influence of closing or non-closing of the peritoneum on short-term postoperative morbidity. The patients operated because of uterine myomae were the participants of our study. Total abdominal hysterectomy with suturing or non-closing of visceral and parietal peritoneum was performed. Our study revealed that non-closing of the peritoneum did not increase the amount of postoperative complications and it seemed to be a safe procedure.

Adult↗
PubMed2003

[Assessment of serum lipid peroxide levels and antioxidant status in females who had undergone total abdominal hysterectomy without closing of the peritoneum].

INTRODUCTION: Reactive oxygen species (ROS) generated after the trauma caused by surgical intervention, have the capacity to react in an indiscriminate manner leading to damage to almost any cellular component (proteins, nucleid acids) and to lipid peroxidation. The assessment of ROS generation intensity process may help in understanding of molecular processes observed in the intracellular environment after operations. The evaluation of antioxidative level may give an answer about the efficiency of antioxidative defence mechanism. The peritoneum is an organ of high metabolic activity, connected with ROS generation and participated in healing process. The modern opinions suggest closing of postoperative wound without closing of peritoneum. The aim of this study was assessment of antioxidant status and serum lipid peroxide levels in early postoperative period, in females who had undergone total abdominal hysterectomy without closing of peritoneum. MATERIALS AND METHODS: 30 women after total abdominal hysterectomy with salpingooophorectomy were studied, including 13 patients after peritoneal suturing (control group--C) and 17 women with nonclosed peritoneum (study group--S). The total serum antioxidant status (FRAP) was evaluated spectrophotometrically. The concentration of lipid peroxidation products was assessed in serum collected before operation and 8 and 24 hours after, as the concentration of lipid hydroxyperoxides (HPETE), malonyl dialdehyde (MDA) and 4-hydroxyalkenals (4-HDA). RESULTS: The antioxidative level before operation was 1147.09 +/- 93.6 microM/l in S group; 1022.04 +/- 115.4 microM/l in C group and did not changed significantly in early postoperative period. MDA + 4-HDA level before operation was 1.08 +/- 0.2 microM/l in S group and 1.06 +/- 0.2 microM/l in C group; HPETE level before operation was 4.58 +/- 0.1 microM/l in S group and 4.72 +/- 0.1 microM/l in C group, 8 and 24 hours after the operation MDA + 4-HDA level increased respectively to 1.34 +/- 0.2 microM/l (by 27%; p > 0.05) and 1.46 +/- 0.2 microM/l (37%) in S group and to 1.16 +/- 0.2 microM/l (by 8%) and 1.62 +/- 0.2 microM/l (by 52%; p < 0.05) in C group. HPETE level decreased to 4.56 +/- 0.1 microM/l (by 1%) and increased to 5.23 +/- 0.2 microM/l (by 14%) in S group and increased respectively to 6.0 +/- 0.2 microM/l (by 27%; p < 0.01) and to 6.43 +/- 0.2 microM/l (by 36%; p < 0.01) in C group. CONCLUSIONS: The concentration of lipid peroxidation products after the operation was lower in study group, where peritoneum was left without closing. Total antioxidative level was the same in both groups. Obtained results suggest smaller ROS generation in study group.

Biomarkers↗
PubMed2006

Changes in collagen and elastic fiber contents of the skin, rectus sheath, transversalis fascia and peritoneum in primary inguinal hernia patients.

BACKGROUND: It has been claimed that inguinal hernia is not a local disease; it is a local manifestation of a systemic disorder of collagen metabolism. Previous studies have shown that patients with inguinal hernia have some anomalies in collagen metabolism and changed ratio of collagen types. AIM: To search the changes in collagen and elastic fiber contents of the skin, rectus sheath, transversalis fascia and peritoneum in primary inguinal hernia patients. METHODS AND MATERIALS: Twenty patients operated on for inguinal hernia (HR) included in the study (11 direct and 9 indirect). Nine patients underwent open cholecystectomy served as the control group (CC). A 0.5 x 1 cm. tissue was sampled from skin, rectus sheath, transversalis fascia and peritoneum in HR group. Skin, rectus sheath and peritoneum samples were taken from the patients in CC group. The sections of those samples were submitted to two different staining methods: "Masson's trichrome" for collagen and "van Gieson" for elastin fibers and graded with light microscopy. RESULTS: The rectus sheath samples of CC had higher staining scores for both collagen and elastin fibers in comparison with HR (p = 0.032 and p = 0.026, respectively). CC had a significantly higher score for collagen in peritoneum samples (p = 0.019). There were no statistically significant differences between the patients with direct and indirect inguinal hernias for collagen or elastin fibers scores in skin, rectus sheath, transversalis fascia and peritoneum samples. CONCLUSIONS: These findings, which concur with most of the previous studies, support the theory that inguinal hernia may not be merely a local disease and can be more generalized, at least a regional connective tissue disorder. Regarding the difference between direct and indirect hernias, it could not be possible to report a certain answer, and this issue should be considered together with previous quantitative researches and more sophisticated studies may take place in the future (Tab. 2, Fig. 2, Ref. 23).

Collagen↗
PubMed1991

[Changes in the peritoneum of the small intestine and diaphragm in experimental portal hypertension].

Diaphragm and small intestine peritoneum morphology was studied in experimental portal hypertension in rats with the help of luminescent, transmission and scanning electron microscopy techniques. Structural organizations of these peritoneum portions and performance function were different: fluid transudation realized through the small intestine peritoneum and resorption occurred via diaphragm peritoneum. Morphological signs allowed to judge about the increasing of fluid transudation in abdominal cavity and diaphragmatic resorption in early period of portal hypertension. Morphological alterations appeared in peritoneum resorption sites (pumping diaphragmatic hatchs) according to progress of portal hypertension that indicated decompensation process of peritoneal fluid absorption and led to ascites.

Animals↗
PubMed1990

[The human peritoneum and human peritonitis in ultrastructural and immunohistochemical studies].

This paper describes observations of the normal and irritatingly changed morphology of the human peritoneum and about alterations in human peritonitis. The results were obtained by transmission- and scanning electron microscopy, immunohistochemical reactions, and other supplementary light microscopic investigations on surgically collected tissue material. Special attention was given to the superficial structure and the openings of the peritoneum. The superficial liquid film and the microvilli of the mesothelium are influenced by irritative conditions. In the human peritoneum, there are separated areas of small high and of large flat mesothelial cells. The small-cell areas of the peritoneum are connected with openings, special mesothelial cell glades, and cell migrations from deeper parts. There are signs of mesothelial secretion into the peritoneal cavity from mesothelial cells and intercellular spaces of the mesothelium too. The stomata--openings of the peritoneum--are of conditioned character. These depends upon the state of the superficial mesothelium and upon topical loci between the mesothelial cells. Special attention requires the so-called Dreierpunkt. In peritonitis, there are also openings in the fibrin layer. On this condition, the superficial layer of mesothelium mostly becomes destroyed. The keratin reaction is the most essential among the immunohistochemical investigations. It allows to demonstrate preserved mesothelial cells in the deepness of inflammatory tissue in peritonitis.

Adolescent↗
PubMed1989

[Experimental studies on the development of peritoneal adhesions in cases of suturing and non-suturing of the parietal peritoneum in rabbits].

The author performed (in general ether anaesthesia) median upper and lower right oblique laparotomy in 50 rabbits (two incisions in each). In 25 rabbits from group I the author closed the wound without suturing the parietal peritoneum whereas in the remaining 25 rabbits from group II they closed the wound suturing the parietal peritoneum. On 1st, 3rd, 7th, 14th and 21st days following the procedure, 5 rabbits from each group were killed. The author evaluated the wounds of the parietal peritoneum by macro- and microscopy, and also the number of peritoneal adhesions in each group. It was found that in group I adhesions appeared only in 3 out of 50 incisions whereas in group II--17 adhesions out of 50 wounds (p less than 0.005). The author believes that parietal peritoneum that has not been sutured heals better and with a smaller number of adhesions than sutured parietal peritoneum.

Abdominal Muscles↗