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Ovulation detection and timing of insemination based on hormone concentrations, vaginal cytology and the endoscopic appearance of the vagina in domestic bitches.

The fertilization period, the time when fertilization can occur, can best be determined using a combination of vaginal cytology and morphology of the vaginal mucosa, behaviour and endocrinology. Neither vaginal cytological changes nor standing oestrus are reliable indicators of the fertilization period per se although with natural mating or when using AI with fresh semen there may be enough biological leeway for pregnancy to follow insemination timed using these parameters alone. This is indicated by the well recognized fecundity of bitches mated early or late in oestrus. Precise discrimination of the fertilization period, which is often required and is necessary for AI using frozen semen, can be achieved by using vaginoscopy to detect the onset of shrinkage with angulation of the mucosal folds. Vaginoscopy is also useful to determine the probable end of the fertilization period and the transition to a 'metoestrous' vaginal smear is also useful in this regard. Detection of the LH peak enables the start of the fertilization period to be forecast quite precisely but, in lieu of practical assays with which to measure plasma LH, a rise in plasma progesterone concentration to 6-8 ng/ml may be more practical.

Animals↗

Accuracy of basal body temperature for ovulation detection.

In 30 normally menstruating women, ages 19 to 41 (mean 24), gravida 0 to 5 (mean 0.7), basal body temperature (BBT) was correlated with serum luteinizing hormone (LH), progesterone, and estradiol or urinary estrogen levels assayed serially during one menstrual cycle. In 21 subjects (70%), a biphasic BBT correlated with an ovulatory hormonal pattern. Six women (20%) had a monophasic BBT but demonstrated a preovulatory estrogen peak, a midcycle LH surge, and a significant rise in serum progesterone levels during the luteal phase. The remaining three women (10%) showed anovulatory cycles (two women) or a deficient luteal phase (one woman) as determined by BBT and hormonal assays. The results indicate that in approximately 20% of ovulatory cycles the BBT failed to demonstrate ovulation.

Adult↗

A prototype for ovulation detection: pros and cons.

A noninstrumented enzyme immunoassay for urinary estrone conjugates was adapted from an instrumented microtiter plate enzyme immunoassay assay. The end point of the assay was a color change from green to clear, which was visible to the unaided eye. The visible color change was adjusted to allow 80 ng/ml estrone conjugates (on the basis of a sample size of 6.5 microliters urine) to be distinguished from an infinite dilution without instrumentation. The evaluation of human urine collected from ovulatory ovarian cycles demonstrated that early follicular phase concentrations (35.9 +/- 6.8 to 79.4 +/- 14.7 ng/ml, n = 10) produced a dark-green color, whereas late follicular phase concentrations (162.9 +/- 20.1 ng/ml, n = 10) produced no color. Daily urine samples throughout 10 ovulatory ovarian cycles produced parallel profiles when compared to measurements of estradiol in paired blood samples. Complete analysis of the data indicated that ovarian follicular dynamics can be accurately monitored through the noninstrumented analysis of daily estrone conjugates in urine samples.

Adult↗

Non-invasive hormonal analysis for ovulation detection.

The present study suggested that measurement of LH, E2 and P in blood may be replaced by non-invasive measurements of E2 and P in saliva and by assays of LH, E1-3G and P2-3G in urine. These alternative methods for determination of reproductive hormones in saliva and in urine provide similar and reliable information of endocrine status in both normal and abnormal reproductive functions. The advantages of these non-invasive methods should be brought into consideration whenever the physiological assessments or clinical investigations of ovarian functions are required.

Adult↗

[Sonographic prediction and detection of ovulation].

The aim of the study was to define the role of sonography as a separate method in prediction and confirmation of ovulation. The growth of the follicle over the period from the 10th to the 17th day of menstruation was sonographically followed-up in 63 spontaneous and induced cycles. It was found that sonography was a quick and simple method. As separate method it can be used for the confirmation of ovulation, prevention of hyperstimulated ovarian syndrome and determination of the best time for artificial insemination. In combination with the other procedures it is convenient for prediction of ovulation.

Female↗

Detection of ovulation by a single endometrial aspiration smear. A preliminary report.

An attempt was made to detect ovulation in 106 normally menstruating women by studying a single endometrial aspiration smear taken just before an endometrial biopsy or D & C on about day 24 +/- 2 of the menstrual cycle. The interpretation of the smears was compared with the histology, and a correlation was observed in 92% of the cases. It is therefore concluded that a single endometrial aspiration smear can be used to detect ovulation. It is an easy, quick, accurate and practical method and can be repeated as often as required.

Biopsy, Needle↗

The prediction and/or detection of ovulation by means of urinary steroid assays.

Twenty normally menstruating women volunteered for a study in which plasma samples were collected daily during an entire menstrual cycle. On the same days, samples of morning urine were also collected, as well as random samples of urine voided at the visit to the Outpatient Clinic. Progesterone (P), estradiol (E2) and lutropin (LH) were assayed in plasma, and pregnanediol-3-glucuronide (PdG), estrone-glucuronide (E1G), estriol-16-glucuronide (E3G), P, and E2 were measured in urine using radioimmunoassays. Progesterone in urine was assayed both with and without preceding chromatography. All urinary glucuronides and progesterone exhibited cyclic patterns similar to those of E2 or P in plasma. Seven-fold increases from early follicular to luteal phase values (for PdG and urinary P; the latter both with and without chromatography), or to peak levels (for E1G and E3G) were observed. The difference between the baseline and peak levels was less distinct (approximately 5-fold) for E2 in urine. The day-to-day coefficient of variation of early follicular phase values decreased from 40% to 25% by calculating the ratios of the glucuronides or P to creatinine (C). The peaks of estrogen glucuronides were delayed mostly by 1 day in comparison to the peaks of E2 in plasma. The urinary peaks of estrogens were in most cases more closely clustered around the day of the LH-peak when the measurements were corrected for C. For the determination of the first significant rise of steroid levels in a cycle, the calculation of a sustained rise (leading to a significant cumulative sum - CUSUM) was found superior when compared to other recommended indices, such as a 50% increase over the mean of 3 preceding values, or the increase over the baseline level plus 2 standard deviations. Sustained rises were calculated for all indices studied (including the ratio of urinary E1G to PdG). The ratio of E1G to C in morning urine gave consistently the most compact distribution of sustained rises. It is concluded that daily measurements of urinary PdG (or P) and E1G (or, possibly, E2) could substitute the serial assays of P and E2 in peripheral blood in the retrospective assessment of the ovarian functionn. The day-to-day variation can be significantly reduced, if results are expressed per concentration of C. For the prediction of ovulation or fertile period, the best index of urinary steroids appears to be the sustained rise in the ratio of E1G to C. However, this "best" method is still not good enough in terms of overall reliability and practicability.

Adult↗