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At least 55 records · Page 3Linked to original sources
PubMed2003

Comparison of several point-of-care testing (POCT) glucometers with an established laboratory procedure for the diagnosis of type 2 diabetes using the discordance rate. A new statistical approach.

The applicability of point-of-care testing (POCT) glucometers for monitoring blood glucose concentrations has been demonstrated. However, their use in diagnosing type 2 diabetes is still debated. Therefore, a new statistical procedure for estimating discordance rates (DRs) was applied in comparing a well-established laboratory method (Ebio) with another laboratory method (Cobas Integra 700) and with several POCT glucometers (Accu-Chek, Accutrend, Elite, HemoCue, Omni) in detecting glucose intolerance states. All procedures led to parallel glucose concentration patterns in capillary blood, venous plasma, and venous blood during oral glucose tolerance tests. However, the mean concentrations differed more or less. The Ebio and Integra results agreed within a maximal deviation of 3%. In blood samples, the HemoCue and Accutrend results were closest to the laboratory procedures (Ebio and Integra) and the highest differences were obtained with the Elite. Comparing whole blood values with those obtained in the aqueous blood compartment (Omni), even greater differences were observed. When all procedures were referred to the same glucose standard, the Ebio, Integra, Accutrend, and Omni results remained almost unchanged, whereas the Elite "moved" toward the Ebio results, and the Accu-Chek results toward the Omni results. Thus, traceability to an aqueous standard was observed with the Ebio, Integra, Accutrend, and Elite in all three sample systems. The Accu-Chek was only traceable in the presence of albumin, and HemoCue was not traceable at all. The clinical relevance of the differences observed between Ebio and POCT glucometers was tested by comparing the relative number of discordant classifications. The highest DRs were observed in the fasting state. They were higher in capillary blood than in the other sample systems. The DRs were found higher with POCT glucometers than with the other established laboratory procedure (Integra). Thus, at least in the fasting state, all POCT glucometers were less reliable than the established laboratory procedures and above the chosen criteria of clinical acceptability (DR < or = 5%). After transforming all glucometer results with a regression function (bias correction), the DRs were less than 5% if compared with the Ebio procedure in all sample systems. In conclusion, the WHO recommendation not to use POCT glucometers for diagnosing type 2 diabetes must be supported. However, after proper recalibration, the tested systems were acceptable. Therefore, manufacturers should reconsider their calibration procedure. Those POCT procedures should be preferred that can be referred to aqueous glucose solutions.

Blood Glucose↗
PubMed1994

Office laboratory procedures, economics of practice, patient and parent education, and urinary tract infection.

This review highlights recent advances in four major areas that are relevant to office practice: office laboratory procedures, economics of practice, adolescent risk-taking behavior in terms of sexually transmitted diseases, and urinary tract infections. Who should be screened for diseases and where these screening tests should be done are addressed, keeping the practicing pediatrician in mind. Next we review current office economics, including whether professional courtesy should be continued, how our practices are going to be increasingly influenced by guidelines developed by the American Academy of Pediatrics, the Clinical Laboratory Improvement Amendments, and the new Clinton Health Plan if it survives Congress, and finally how all of these issues will affect our expected income in the years ahead. As pediatricians strive to retain adolescent patients in their practices, they will need to find appropriate ways of counseling these patients concerning risk behaviors that could result in sexually transmitted diseases or HIV infections. Should we leave the comfortable confines of our offices to participate in these counseling programs for adolescents, and are there lessons from existing successful International Health Programs that we can use? Finally, urinary tract infections (UTIs) continue to be a common cause of childhood infections with possible serious long-term sequelae. Can we do a better job of diagnosing UTIs, has improved treatment become available, and is prevention of recurrences possible? Once the diagnosis has been made, how can we best evaluate these children with UTIs for underlying urologic abnormalities? It is our hope that the practicing pediatrician will be better prepared to face these issues having read this review.

Adolescent↗
PubMed1985

Clinical praxis and laboratory procedures in subrenal capsule assay (SRCA).

Subrenal capsule assay (SRCA) is a promising method in cancer research and in the selection of individual chemotherapy for cancer patients. The laboratory procedures of SRCA are as follows: on day zero 1 X 1 X 1 mm pieces of fresh human tumour are carefully prepared. The pieces are transferred by a trocar under the outer capsule of normal immunocompetent mice, one piece to each. The mice (about 30-40/test) are divided randomly into groups of at least five. The size of each piece (initial size) is measured in situ by a stereomicroscope fitted with an ocular micrometer. On days 1-5 cytostatics are administered to the animals. On day 6 the animals are killed and the final tumour size is measured. The difference between the final and initial tumour size describes the response of the tumour to the treatments. The critical point of the assay is the selection of the tumour fragment which has to be representative, as homogeneous as possible and contain living tumour cells. When the sample is in pieces of about 3 X 3 mm (4-5 tumour fragments are sufficient for the assay) in the culture medium, it may be stored in an ice bath or at room temperature for one day. During this time, though as rapidly as possible, the sample has to be delivered to a research laboratory where the SRCA is carried out.

Animals↗
PubMed2006

Correct coding for laboratory procedures during assisted reproductive technology cycles.

New Current Procedural Terminology (CPT) codes for 2004 have been adopted for utilization with assisted reproductive technologies. It is important for professionals in the field of assisted reproductive technology (ART) to become familiar with them prior to their implementation. This document replaces the July 2002 American Society for Reproductive Medicine (ASRM) Practice Guideline Correct Coding for Laboratory Procedures During Assisted Reproductive Technology.

Clinical Laboratory Techniques↗
PubMed1989

[Displacement and distortion of non-precious ceramometal fixed bridges during the laboratory procedure. Non-precious metal].

The purpose of this study is to measure the three-dimentional displacement and distortion in the Non-precious (Ni-Cr) ceramometal bridges during laboratory procedure. At the time of pre-soldering stage, the author examined the phosphatic investment (low and high thermal expantion: ph, ps) to addition of gypsum investment which was usually to used to fix the position for soldering. The following results were obtained. 1. The displacement of the framework at the pre-soldering stage, in case of using phosphatic investment mixed by water (ph), it was small on the horizontal and vertical plane. And in the other case of mixing by 100% colloidal silica (ps), a kind of displacement was seen where the curve of the bridge opened as result of the position of two soldering bodies getting out of order from each other. So that phosphatic bonded investment, it can be used to because thermal expantion can be controlled, the soldering block. 2. Throughout the surface grinding of framework to grazing, displacement was minimal, at the time of opaque porcerain firing, the curve of the bridge closed but it had no effect on clinical adaptation. 3. In the case of assembling multi-unit non-precious ceramometal bridge mainly the displacement caused at pre-soldering stage, so it is important to check the adaptation after soldering.

Chromium Alloys↗
PubMedDate not supplied

Brånemark Novum: prosthodontic and dental laboratory procedures for fabrication of a fixed prosthesis on the day of surgery.

PURPOSE: The purpose of this report is to describe a new technique to fabricate and deliver an implant-supported fixed prosthesis to the patient on the day of surgery, and to propose a protocol for the prosthodontic and dental laboratory procedures. MATERIALS AND METHODS: The development of the Brånemark Novum prosthodontic protocol is reviewed, and clinical and dental laboratory assessments and methods are described. RESULTS: The total treatment time to fabricate a permanent implant-supported fixed prosthesis in the mandible can be reduced to 1 day with the Brånemark Novum technique. The new method includes a precise surgical technique using drilling templates for predetermined implant positions, a rigid splinting of the implants immediately after placement, the use of a prefabricated titanium framework, and elimination of implant impression procedures. CONCLUSION: With the technique described in this report, it is possible to provide patients with a permanent implant-supported fixed prosthesis in the mandible on the day of implant surgery. Patient benefits are obvious, with drastically reduced total treatment time, lower cost, and fewer clinical visits.

Dental Implantation, Endosseous↗
PubMed2003

Recent advances in laboratory procedures for pathogenic mycobacteria.

Just as tuberculosis has persisted for many centuries as one of most serious and deadly infectious diseases in many parts of the world, so has the motivation to develop improved laboratory methods for characterizing M. tuberculosis isolates. Modern technology has lead to great improvements in mycobacteriology laboratory procedures, particularly in detection, identification, epidemiologic strain typing, and drug susceptibility testing. Although the usefulness of some of these newer methods is under evaluation, many already are showing potential as adjuncts to clinical diagnostic procedures.

Genotype↗
PubMed1998

Bond strength of photocured composite resin facings: clinical versus laboratory procedures.

The tensile strengths of laboratory versus clinical photocured composite resins have been investigated. Metal surfaces were bonded to photocured composite resin by either retentive beads, Sebond or Silicoating. The bond strengths were measured by an Instron machine and the fracture sites observed. Following repair with the adhesives Dentacolor Opaquer, Fusion and Cover up 2, the Instron measurements were repeated. The metal/facing bond strength was the highest in samples fabricated by the Silicoating technique, the bond strength exceeding the cohesive forces in the composite resin facing. The tensile strengths of metal/facing bonded by Sebond and retentive beads were similar. Fractures that include the facing and partially reveal the metal are the least resistant to tension after repair. Renewal of the Opaque layer and completion of the facing is superior to any of the repair methods used in this paper. The original fracture point is the weakest after repair. All the repair kits tested were inferior to the original restoration materials.

Adhesiveness↗
PubMed2004

Correct coding for laboratory procedures during assisted reproductive technology cycles.

New Current Procedural Terminology (CPT) codes for 2004 have been adopted for utilization with assisted reproductive technologies. It is important for professionals in the field of assisted reproductive technology (ART) to become familiar with them prior to their implementation. This document replaces the July 2002 American Society for Reproductive Medicine (ASRM) Practice Guideline Correct Coding for Laboratory Procedures During Assisted Reproductive Technology.

Clinical Laboratory Techniques↗
PubMed1995

Stereo laser-welded titanium implant frameworks: clinical and laboratory procedures with a summary of 1-year clinical trials.

A new technology for implant framework fabrication (Procera) has been introduced to North America after initial clinical trials in Sweden. This technique is unique because it eliminates the conventional approach to framework fabrication with the lost wax casting technique. Clinical and laboratory procedures associated with the Procera technique are described in this article, as are the most recent developments in this rapidly emerging technology. A report of 1-year results of the first 10 patients treated with the Procera technique at the University of Washington is described. Treatment outcome has been favorable, with no clinical evidence of prosthodonic or soft-tissue complications noted. All 50 of the implants placed in the 10 patients remain integrated after 1 year of service. The Procera technology offers an alternative to current conventional framework fabrication techniques. Continued follow-up of this patient series is pursued to explore the treatment outcome for a longer interval of time.

Aged↗
PubMed1998

Adhesive crowns and fixed partial dentures fabricated of ceromer/FRC: clinical and laboratory procedures.

Ceramic optimized polymer and fiber-reinforced composite materials represent a significant development in prosthetic dentistry. When utilized in conjunction with adhesive luting techniques, exceptionally conservative crown and bridge restorations may be achieved. This article discusses utilization of these materials in inlay and onlay restorations, as well as clinical and laboratory procedures for fabrication, preparation, and seating of adhesive crown and bridge restorations. The initial results of restorations utilizing these innovative materials are presented.

Ceramics↗
PubMed2004

Correct coding for laboratory procedures during assisted reproductive technology cycles.

New Current Procedural Terminology (CPT) codes for 2004 have been adopted for utilization with assisted reproductive technologies. It is important for professionals in the field of assisted reproductive technology (ART) to become familiar with them prior to their implementation. This document replaces the July 2002 American Society for Reproductive Medicine (ASRM) Practice Guideline Correct Coding for Laboratory Procedures During Assisted Reproductive Technology.

Cell Culture Techniques↗
PubMed1977

Enzymatic degradation of polygalacturonic acid by Yersinia and Klebsiella species in relation to clinical laboratory procedures.

As scored by several specified plating procedures, clinical and environmental strains of Yersinia enterocolitica, Yersinia pseudotuberculosis, and Klebsiella pneumoniae "Oxytocum" showed detectable, albeit generally weak, ability to digest polygalacturonic (pectic) acid. None of these bacterial strains had the vigorous and rapid pectolytic activity on these polygalacturonic acid-containing media that is typical of soft-rot Erwinia species, although some of the Oxytocum strains came fairly close. Analyses of the pectolytic enzyme contents of the cells and culture supernatants of the Yersinia and Klebsiella species revealed that readily detectable quantities of cell-bound polygalacturonic acid trans-eliminase and hydrolytic polygalacturonase were formed by the Yersinia and Klebsiella species; however, the total units of enzyme activity produced by these bacteria were, in general, lower than were produced by soft-rot Erwinia species. Furthermore, unlike the situation in soft-rot Erwinia cultures, these pectolytic enzymes of Yersinia and Klebsiella species were not excreted rapidly and massively into the growth medium. Cultures of other enterobacteria (Citrobacter species, Enterobacter species, Erwinia amylovora, Erwinia herbicola, Escherichia coli, Proteus species, Salmonella typhimurium, and Serratia marcescens) showed no pectolytic ability whatsoever by any of the plating procedures used and (to the extent they were so examined) produced no pectolytic enzymes detectable either in their cells or culture supernatants. This slow or weak release of pectolytic enzymes by Yersinia and Klebsiella species has a bearing on clinical laboratory procedures suitable for detecting their pectolytic activity; methods adequate for this purpose are detailed.

Bacteriological Techniques↗