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[Preparations for serodiagnosis of diseases due to causative agents of ixode tick-borne Borreliosis (Lyme disease). Communication 2. Comparative study of recombinant enzyme immunoassay test-systems (rELISA) for serological diagnosis of ixode tick-borne Borreliosis].

One foreign and two Russian recombinant enzyme immunoassay test-systems were comparatively investigated under conditions of an encoded experiment. Sensitivity in the experiment with the Russian test-systems Borreliosis-ELISA-IgG and Lyme Best was 63.8 and 68.8% respectively. As for the test-system Borrelia IgG Recombinant, it was 47.5%. All the test-systems were highly specific (94.4 to 99.5%). The test-systems Lyme Best and Borrelia IgG Recombinant revealed partial cross reactions with sera from patients with systemic lupus erythematosus and leptospirosis.

Antibodies, Bacterial↗

[Genetic characteristics of human pathogenic Borrelia isolated from Ixodes trianguliceps Bir. and Ixodes pavlovskyi Pom].

To support the results of typing the amplicon of the spacer site 5S-23S of pRNA by restriction fragment length polymorphism (RFLP) assay, the isolates from the ticks I. trianguliceps and I. pavlovskyi underwent sequenation of rrf (5S)-rrl (23S) spacer to make a comparative analysis of derived nucleotide sequences with the DDBJ/EMBL/ GenBank databases. For this purpose, the authors used six isolates (three imago and three nymphal) from I. trianguliceps and three isolates from adult hungry I. pavlovskyi females from the Borrelia museum of the Laboratory of Infection Transmitters, N. F. Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences. In the natural foci of the Perm Region, in the tick I. trianguliceps the same genetic variants of B. afzelii VS461 and B. garinii NT29 was found to occur as in I. persulcatus and small mammals, the reservoir Borrelia hosts. Borreliae previously detected in the tick I. pavlovskyi belong to B. garinii 20047.

Animals↗

[A case of bringing along Mediterranean ticks Ixodes (Ixodes) festai Rondelli, 1926 (Acari: Ixodida: Ixodidae) to Poland by migrating birds].

A partly fed female of I. festai was collected from Turdus merula L. caught in Hel Peninsula (Southern Coast of Baltic Sea). This species (known only as females) is a parasite of birds, and inhabits western part of the Mediterranean area. I. festai is a second species after Hyalomma marginatum which probably every spring is transferred by migratory bird into territory of Poland. This finding is the first recorded case of transfer of I. festai to Poland, therefore the description of female is given.

Animals↗