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Epoxide hydrase activity in mouse skin eidermis.

By fractionation of mouse epidermis a "microsomal" fraction may be obtained with a specific EH-activity at least 6-7 times higher than that of epidermis homogenates. Such enrichment allows to utilize the radioassay with 7--3H-styrene oxide as substrate which normally is too insensitive for organs with low levels of EH-activities. By the procedure developed the unambiguous demonstration of EH-activity in the epidermis of mouse skin is possible.

Animals↗

Effect of vitamin A deficiency on rat hepatic and colon epoxide hydrase.

Rat liver microsomes and homogenized mucosal linings prepared from vitamin A-supplemented and deficient male rats were used in metabolic studies of 7-3H-styrene oxide. The colon tissue in deficient animals exhibits a significantly higher value of Vmax than the same tissue from vitamin-supplemented animals. The implications of this finding in addition to our earlier observation 10 is discussed in relation to colon carcinoma.

Animals↗