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Extensive new high-temperature, high-resolution FTIR emission spectroscopy measurements for the five common isotopomers of GeO are combined with previous diode laser and microwave measurements in combined isotopomer analyses. New Dunham expansion parameters and an accurate analytical potential energy function are determined for the ground X1Sigma+ state. Copyright 1999 Academic Press.
The Thailand 'Rural Data Base' includes a number of variables in the social, economic, services, housing, agriculture and health fields. This data base comes from a survey at the village level. There are weaknesses in the data base--inaccuracies, missing data, etc ... that make it difficult to discover the structures that theoretically exist in the data using direct analysis methods, like correlation analysis for instance. The 2383 villages of the province of Ubon-Ratchathani, in Thailand, are the units of this study. The objective is to uncover the hypothetical health disparities that exist among the villages and to devise a typology of villages that will show classes of villages that have significant problems and, also, classes of villages that could suggest concrete solutions and be used as models. Some variables of the data base are considered dependent (health indicators) and others are considered independent; they are used to build a model called 'geo-systemic' that is used to expose mortality differentials in Ubon-Ratchathani.
The aim of this paper is to pilot a method for geo-demographic classification for mortality patterns in Britain. Age and sex directly standardised mortality ratios (DSMRs) for 100 grouped International Classification of Disease series 9 causes of death (ICD-9) were calculated. The 84 European Parliamentary (EP) constituencies as defined in 1999 were used as the spatial basis of this study to allow regional comparisons to be made while comparing units of similar population sizes. Scotland was excluded from the final analysis, leaving 76 regions. This paper is a preliminary investigation of the patterns of the causes of death over time and space in England and Wales using cluster analysis to summarise some of the structure in the data. Seven major and three minor cluster profiles were developed.
Er(3+)/Yb(3+) co-doped 60Bi(2)O(3)-(40 - x)B(2)O(3)-xGeO(2) (BBG; x=0, 5, 10, 15 mol%) glasses that are suitable for fiber lasers, amplifiers have been fabricated and characterized. The absorption spectra, emission spectra, and lifetime of the (4)I(13/2) level and quantum efficiency of Er(3+):(4)I(13/2) --> (4)I(15/2) transition were measured and calculated. With the substitution of GeO(2) for B(2)O(3), both Delta lambda(eff) and sigma(e) decrease from 75 to 71 nm and 9.88 to 8.12 x 10(-21) cm(2), respectively. The measured lifetime of the (4)I(13/2) level and quantum efficiency of Er(3+):(4)I(13/2) --> (4)I(15/2) transition increase from 1.18 to 1.5 ms and 36.2% to 43.2%, respectively. The emission spectra of Er(3+):(4)I(13/2) --> (4)I(15/2) transition was also analyzed using a peak-fit routine, and an equivalent four-level system was proposed to estimate the stark splitting for the (4)I(15/2) and (4)I(13/2) levels of Er(3+) in the BBG glasses. The results indicate that the (4)I(13/2) --> (4)I(15/2) emission of Er(3+) can be exhibit a considerable broadening due to a significant enhance the peak A, and D emission.
The ROSA beta geo26 (ROSA26) mouse strain was produced by random retroviral gene trapping in embryonic stem cells. Staining of ROSA26 tissues and fluorescence-activated cell sorter-Gal analysis of hematopoietic cells demonstrates ubiquitous expression of the proviral beta geo reporter gene, and bone marrow transfer experiments illustrate the general utility of this strain for chimera and transplantation studies. The gene trap vector has integrated into a region that produces three transcripts. Two transcripts, lost in ROSA26 homozygous animals, originate from a common promoter and share identical 5' ends, but neither contains a significant ORF. The third transcript, originating from the reverse strand, shares antisense sequences with one of the noncoding transcripts. This third transcript potentially encodes a novel protein of at least 505 amino acids that is conserved in humans and in Caenorhabditis elegans.
PURPOSE: Epidermal growth factor receptor (EGFR), a protein tyrosine kinase expressed in many types of human cancers, has been strongly associated with tumor progression. Cetuximab is an IgG(1) anti-EGFR chimeric mouse/human monoclonal antibody that has been approved for the treatment of advanced colon cancer. Using human tumor xenografts grown in nude mice, we have determined the in vivo pharmacodynamic response of cetuximab at efficacious doses. Three pharmacodynamic end points were evaluated: tumoral phospho-EGFR, tumoral mitogen-activated protein kinase (MAPK) phosphorylation, and Ki67 expression. EXPERIMENTAL DESIGN: The pharmacodynamic study was conducted in nude mice bearing Geo tumors following a single i.p. administration of 0.25 and 0.04 mg. The tumors were analyzed by immunohistochemistry. The levels of phospho-EGFR were quantitated by an ELISA assay. RESULTS: At 0.25 mg, phospho-EGFR was maximally inhibited by 91% at 24 hours, whereas the level of inhibition decreased to 72% by 72 hours. At 0.04 mg, the maximum inhibition of phospho-EGFR was 53% at 24 hours, whereas the level of inhibition decreased to 37% by 72 hours. The time course of phospho-EGFR inhibition and recovery seemed to correlate with the pharmacokinetics of cetuximab. Immunohistochemical analysis showed that phospho-MAPK and Ki67 expression were inhibited between 24 and 72 hours at 0.25 and 0.04 mg. A pharmacokinetic/pharmacodynamic model was established and predicted that the plasma concentration of cetuximab required to inhibit 90% of phospho-EGFR was 67.5 mug/mL. CONCLUSIONS: Phospho-EGFR/phospho-MAPK could be useful clinical biomarkers to assess EGFR inhibition by cetuximab.
The automatic alignment system for the two suspended, triangular mode-cleaner cavities of the gravitational wave detector GEO 600 has been in continuous operation since the spring of 2001. A total of 20 angular degrees of freedom are controlled by feedback loops with almost no manual alignment having been required since installation. Error signals for the eight most important degrees of freedom are obtained with the differential wave-front sensing technique. We describe the sensing system, the control topology, and the performance of the automatic alignment system. A continuous locking stretch of more than 120 h has been achieved.
Antibody in human sera that induces lysis of sheep erythrocytes in hemolytic assay was investigated. The present study showed that the presence in serum of the thermostable cytolytic anti-sheep red blood cells antibodies is dependent on the Schistosoma mansoni infection, and this is more frequent in adults than in children. The thermostable characteristic of hemolysins in normal sera was not dependent on the presence of Ascaris lumbricoides, Trichuris trichiura or hookworm geo-helminths. Further, thermostable complement-activating heterophile antibodies were noticed in children in association with massive number of S. mansoni eggs. The results were obtained by using the z- and the chi-square tests. The z-test allows us to formulate a one-sided alternative, i.e., a tendency of one of the attributes. On the other hand, the chi-square test analyzes the independence between attributes by using a contingency table. Besides the obtained results being interesting in the field of schistosomiasis mansoni, they can provide a new insight into the use of statistics in medical science.
The descriptive epidemiology and geo-pathology of malignant lymphomas have been hampered by the absence of a unified classification. Recent proposal of the REAL classification which will soon become the WHO classification is a significant step toward better understandings of the epidemiology of this interesting neoplasia. Worldwide epidemic of non-Hodgkin's lymphoma was discussed with possible relationships to herbicides. General characteristics of Japanese lymphomas as compared to Western lymphomas were described with special emphasis on the correlation between the low incidence rates of follicular lymphomas and Hodgkin's lymphomas.
OBJECTIVE: To study the relationship between the Geo-authentic and Geologic Background System (GBS) and physicochemical properties of cultivated soil in the Flos Lonicerae. METHOD: The analysis on the physicochemical properties of cultivated soil, the geologic background system in 5 producing areas of Flos Lonicerae was compared. RESULT: The distribution of genuine Flos Lonicerae was restricted by GBS, which was mainly restricted in the temperate continental monsoon semi-arid climate zone between north latitude 34-36 degrees and east longitude 113-118 degrees. CONCLUSION: The most suitable soil for the genuine Flos Lonicerae is the neutral or weak alkali sandy loamy soil with higher ion exchanging capacity, and the soils from those producing areas are determined by soil-forming parent materials.
BACKGROUND AND PURPOSE: Tiagabine (TGB) is a new antiepileptic drug with a unique mechanism of action, with efficacy and tolerance confirmed in many randomized, placebo-controlled add-on trials in patients with partial epilepsy. The main aim of the international GEOS study (Gabitril Epilepsy Observational Study) was to analyze the efficacy and safety of TGB as an add-on therapy in everyday clinical practice. MATERIAL AND METHODS: 260 patients from Poland (1227 in the whole study) were enrolled (mean age 35.0+/-13.7 years). 53% had symptomatic epilepsy, 25% idiopathic, 12% cryptogenic, in remaining 10% of patients the etiology of the epileptic syndrome had not been determined. 69% of patients had complex partial seizures (CPS), 60% of patients had secondary generalized tonic clonic seizures (SGTCS) and 32% of patients had simple partial seizures (SPS). The patients were observed for 12 months. RESULTS: The full study was completed by 201 (76%) of patients. The mean TGB dose received at the end of the study was 31,3+/-12 mg/day. At the end of the study, i. e. after 12 months, 75% patients with SP, 67% patients with SGTCS and 48% patients with CPS were free of seizures. 94% of patients with SPS, 92% patients with SGTCS and 91% of patients with CPS had 50% or more reduction in seizures versus baseline. TGB was well-tolerated, adverse events appeared in 14% of patients and had mild or moderate severity. CONCLUSIONS: The obtained results confirmed that TGB was effective and well-tolerated in everyday clinical practice when given as add-on therapy to patients with partial epilepsy.
One of the crucial steps on the way to individualized medicine to treat cardiovascular disease (CVD) is to better understand the identities, roles, extent and at least the major patterns of interaction between influential genomic and environmental factors. It is clear that such a bold goal can hardly be achieved without a major upgrade of our conceptualization of the phenomena studied, taking advantage of recent developments of novel technological and computational tools. Firstly, the search for the genomic components of the most common multifactorial CVDs is no longer restricted to protein-coding genes; truly genome-wide investigations should replace them in both humans and animal models. Secondly, the 'environment' has also undergone semantic expansion, incorporating such remote constituents as developmental plasticity and epigenetics on one side, and socioeconomic status on the other. To elucidate and analyze the resulting complex picture, appropriate statistical models and approaches need to be designed to tackle issues such as population stratification and admixture, multiple testing, and multidimensionality reduction in models involving multiple genes and environmental factors. Eventually, an integrated platform bringing together all of the above will probably be necessary to secure relevant information specific to a particular combination of conditions and settings (age, geo-ethnicity and exposure), which may perhaps become visible only after a step back, through systems (network) biology.
This study investigates the degree to which gender, ethnicity, relationship to perpetrator, and geomapped socio-economic factors significantly predict the incidence of childhood sexual abuse, physical abuse and non- abuse. These variables are then linked to geographic identifiers using geographic information system (GIS) technology to develop a geo-mapping framework for child sexual and physical abuse prevention.
Nuclear-targeted beta-galactosidase (beta-gal) is increasingly used as a genetic cell marker in vitro and in vivo. Nuclear sequestration concentrates beta-gal and permits sensitive identification of expressing cells and/or tissues without obscuring the cytoplasmic detail necessary for analysis of cell phenotype. Here, we report the construction and testing of a nuclear-targeted version of the beta geo fusion protein that combines nuclear localization with the ability to select expressing cells with the drug G418. This new marker gene functions efficiently in retroviral vectors and will be useful in identification and isolation of cells transfected in vitro and cells expressing transgenic or gene-targeted constructs in vivo.
The detailed structure of locomotor activity rhythm of rats in the ultradian range was investigated. The stability of discovered ultradian periods and their accordance to well-known periods of magnetic AE-index were estimated. It was revealed that a lot of geo- and heliophysically significant harmonics in the ultradian range with different stability are always present in the rhythm of locomotor activity. In most cases, the stable periods are daily harmonics. Such time organization is optimal for living organisms in case of simultaneous use of several "zeitgebers" of different nature, whose periods can substantially vary.
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Sox10 is an essential transcription factor required for development of neural crest-derived melanocytes, peripheral glia, and enteric ganglia. Multiple transcriptional targets regulated by Sox10 have been identified; however, little is known regarding regulation of Sox10. High sequence conservation surrounding 5' exons 1 through 3 suggests these regions might contain functional regulatory elements. However, we observed that these Sox10 genomic sequences do not confer appropriate cell-specific transcription in vitro when linked to a heterologous reporter. To identify elements required for expression of Sox10 in vivo, we modified bacterial artificial chromosomes (BACs) to generate a Sox10betaGeoBAC transgene. Our approach leaves endogenous Sox10 loci unaltered, circumventing haploinsufficiency issues that arise from gene targeting. Sox10betaGeoBAC expression closely approximates Sox10 expression in vivo, resulting in expression in anterior dorsal neural tube at embryonic day (E) 8.5 and in cranial ganglia, otic vesicle, and developing dorsal root ganglia at E10.5. Characterization of Sox10betaGeoBAC expression confirms the presence of essential regulatory regions and additionally identifies previously unreported expression in thyroid parafollicular cells, thymus, salivary, adrenal, and lacrimal glands. Fortuitous deletions in independent Sox10betaGeoBAC lines result in loss of transgene expression in peripheral nervous system lineages and coincide with evolutionarily conserved regions. Our analysis indicates that Sox10 expression requires the presence of distant cis-acting regulatory elements. The Sox10betaGeoBAC transgene offers one avenue for specifically testing the role of individual conserved regions in regulation of Sox10 and makes possible analysis of Sox10+ derivatives in the context of normal neural crest development.
Asthma researchers have found some evidence that geographical variations in susceptibility to asthma could reflect the effect of community level factors such as exposure to violence. Our methodology was motivated by a study of age at onset of asthma among children of inner-city neighbourhoods in East Boston. Cox's proportional hazards model was not appropriate since there was not enough information about the nature of geographical variations so as to impose a parametric relationship. In addition, some of the known risk factors were believed to have non-linear log-hazard ratios. We extend the geoadditive models of Kamman and Wand to the case where the outcome measure is a possibly censored time to event. We reduce the problem to one of fitting a Poisson mixed model by using Poisson approximations in conjunction with a mixed model formulation of generalized additive modelling. Our method allows for low-rank additive modelling, provides likelihood-based estimation of all parameters including the amount of smoothing and can be implemented using standard software. We illustrate our method on the East Boston data.