[Isolation of flavonoids using oxides and salts of bivalent cations, II: sorption of flavonoids to caO, mgO and znO (author's transl)].
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Genista tenera is a plant native to the Madeira Island (Portugal). From the ethanol extract of its powdered aerial parts, two flavones, three isoflavones and one 7-O-glucosyl isoflavone were isolated. A mass spectrometric study of these compounds was performed using liquid secondary ion mass spectrometry (LSIMS) in combination with high-energy collision-induced dissociation (CID) and tandem mass spectrometry (MS/MS). Characteristic fragmentation patterns were observed in all the investigated compounds; the loss of small neutral species from the protonated molecules was useful for identifying the presence of specific functional groups in the A- and B-rings. In order to help to establish the proposed structures, NMR and UV studies were also performed.
A flavone glycoside was isolated from the leaves of Betula platyphylla var. latifolia and characterized as 4',6-Dimethoxy-5-hydroxyflavone-7-O-beta-D-glucoside(pectolinar igenin-7-O -beta-D-glucopyranoside) by method of chemical and NMR spectral analysis. 13C-1H long range coupling was confirmative for determination of its substituted position. In connection with this study, 6-C-Glucosylnalingenin and 6-C-Glucosylaromadendrin were confirmed its structures using this techniques.
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