Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “Cellular Structures”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 55 records · Page 3Linked to original sources
PubMed1997

[Effects of electric fields on the cellular structure of the regenerated nerve].

Eighty adult SD rats were randomly divided into four groups of 20 each: 1. local electrostimulation of nerve stump (Group LS); 2. electrostimulation of myeloneure (Group N); 3. electrostimulation of the denervated muscle (Group M); and 4. Control (Group C). 5 millimeters of the left sciatic nerve of the rat was excised and the severed nerve was bridged with a silicon tube. The gap between the stumps was about 10 millimeters. On the 3rd day and the 1st, 2nd, 3rd, 4th week after surgery, samples were taken and processed for microscopic examinations to observe the effects of electric fields on cellular structural development of regenerated axons, especially on the proliferation and migration of Schwann's cell and fibroblasts, etc. The number of the axon and capillary, the degree of axon maturity and myelinated axon proportion were also observed. The results showed that electrostimulation enhanced nerve regeneration by, 1. promoting the form of matrix bridge; 2. stimulating Schwann's cell and fibroblast migration and proliferation; 3. promoting vascularization of regenerating nerves; and 4. direct electrophoretic effects on structural protein of growth cone.

Animals↗
PubMed1995

Cellular structure and function of rat fat cells in the primary culture.

A primary culture of undigested fat tissue fragments was used to obtain fat cells in vitro. On day 2 of culture, immature fat cells, which are fibroblast-like fat cells containing fine lipid droplets, appeared, surrounding the fat tissue fragments, and began to proliferate extensively. Afterwards, these fibroblast-like fat cells grew to become multilocular fat cells containing larger intracytoplasmic lipid droplets, and differentiated further into unilocular fat cells containing a single large intracytoplasmic lipid droplet. Treatment with dibutyryl-cAMP, which is a second messenger of the lipolytic factor, caused the cultured fat cells to retract, and the intracytoplasmic lipid droplets of those fat cells became finely granulated and decreased along with an increase of hormone-sensitive lipase activities. Conversely, administration of insulin caused the lipid droplets in the fat cells to increase and become larger along with an increase of alpha-glycerophosphate dehydrogenase activities. These findings indicate the occurrence of lipolysis and lipogenesis of fat cells in vitro. Immuno-cytochemistry revealed that vimentin surrounded intracytoplasmic lipid droplets, and became distinct with an increase of lipid droplets through lipogenesis in the fat cells. Vimentin seems to be correlated to the behavior of lipid droplets in the fat cells. Fat cells in this study showed the appropriate cellular structures and functions in response to stimulation of lipolysis and lipogenesis under culture conditions. It is expected that in vitro culture of fat cells will facilitate cell biological elucidation of obesity in the future.

Adipocytes↗