Molecular cloning, nucleotide sequence and structural analysis of the Streptomyces galbus DSM40480 fda gene: the S. galbus fructose-1,6-bisphosphate aldolase is a member of the class II aldolases.
The fda gene of Streptomyces galbus DSM40480 encoding the fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) was cloned, sequenced and characterised. The fda gene encodes a protein of 341 amino acids with a molecular mass of 36.5 kDa and belongs to the class II aldolases. When the S. galbus fda gene was expressed in the Escherichia coli fda(ts) mutant NP315, the growth defect of the strain was complemented at temperatures >35 degrees C. In Northern hybridisations, we identified an fda transcript of 1200 bp length. The transcript length indicates that the fda gene is transcribed from its own promoter. Attempts to isolate fda knock out mutants were not successful. Streptomyces lividans strains with a second copy of the fda gene were constructed and analysed.