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Pseudomonas aeruginosa mutants altered in their sensitivity to the effect of iron on toxin A or elastase yields.

Iron affects yields of toxin A, alkaline protease, elastase, pyochelin, and pyoverdin in Pseudomonas aeruginosa. Mutants of P. aeruginosa PAO1 resistant to the effect of iron on toxin (toxC) or elastase (elaC) yields were isolated. Two types of mutants were isolated: iron transport and iron regulatory mutants. The toxC regulatory mutants produced toxin A in medium containing iron; however, yields of elastase and alkaline protease remained sensitive to regulation by iron. The elaC regulatory mutants were resistant to the effect of iron on elastase yields, but toxin A and alkaline protease yields were decreased by iron, analogous to the parent strain. These data suggest that toxin A, elastase, and alkaline protease yields can be independently regulated by iron.

ADP Ribose Transferases↗

Influence of transport energization on the growth yield of Escherichia coli.

The growth yields of Escherichia coli on glucose, lactose, galactose, maltose, maltotriose, and maltohexaose were estimated under anaerobic conditions in the absence of electron acceptors. The yields on these substrates exhibited significant differences when measured in carbon-limited chemostats at similar growth rates and compared in terms of grams (dry weight) of cells produced per mole of hexose utilized. Maltohexaose was the most efficiently utilized substrate, and galactose was the least efficiently utilized under these conditions. All these sugars were known to be metabolized to glucose 6-phosphate and produced the same pattern of fermentation products. The differences in growth yields were ascribed to differences in energy costs for transport and phosphorylation of these sugars. A formalized treatment of these factors in determining growth yields was established and used to obtain values for the cost of transport and hence the energy-coupling stoichiometries for the transport of substrates via proton symport and binding-protein-dependent mechanisms in vivo. By this approach, the proton-lactose stoichiometry was found to be 1.1 to 1.8 H+ per lactose, equivalent to approximately 0.5 ATP used per lactose transported. The cost of transporting maltose via a binding-protein-dependent mechanism was considerably higher, being over 1 to 1.2 ATP per maltose or maltodextrin transported. The formalized treatment also permitted estimation of the net ATP yield from the metabolism of these sugars; it was calculated that the growth yield data were consistent with the production of 2.8 to 3.2 ATP in the metabolism of glucose 6-phosphate to fermentation products.

Biological Transport, Active↗

Mortality in relation to tar yield of cigarettes: a prospective study of four cohorts.

OBJECTIVE: To investigate relation between tar yield of manufactured cigarettes and mortality from smoking related diseases. DESIGN: Prospective epidemiological study of four cohorts of men studied between 1967 and 1982. SETTING: Combined data from British United Provident Association (BUPA) study (London), Whitehall study (London), Paisley-Renfrew study (Scotland), and United Kingdom heart disease prevention project (England and Wales). SUBJECTS: Of the 56,255 men aged over 35 who were included in the studies, 2742 deaths occurred among 12,400 smokers. Average follow up was 13 years. MAIN OUTCOME MEASURES: Relative mortality from smoking related diseases according to tar yields of cigarettes smoked. RESULTS: Age adjusted mortality from smoking related diseases in smokers of filter cigarettes was 9% lower (95% confidence interval 1% to 17%) than in smokers related diseases consistently decreased with decreasing tar yield. Relative mortality in cigarette smokers for a 15 mg decrease in tar yield per cigarette was 0.75 (0.52 to 1.09) for lung cancer, 0.77 (0.61 to 0.97) for coronary heart disease, 0.86 (0.50 to 1.50) for stroke, 0.78 (0.40 to 1.48) for chronic obstructive lung diseases, 0.78 (0.65 to 0.93) for these smoking related diseases combined, and 0.77 (0.65 to 0.90) for all smoking related diseases. CONCLUSION: About a quarter of deaths from lung cancer, coronary heart disease, and possibly other smoking related diseases would have been avoided by lowering tar yield from 30 mg per cigarette to 15 mg. Reducing cigarette tar yields in Britain has had a modest effect in reducing smoking related mortality.

Adult↗

Contributions of motor-unit recruitment and rate modulation of compensation for muscle yielding.

1. Subdivided portions of the cut ventral root innervation of the soleus muscle were electrically stimulated in 14 anesthetized cats. The stimulus trains imposed on these nerves simulated the recruitment and rate-modulation patterns of single motor units recorded during stretch-reflex responses in decerebrate preparations. Each activation pattern was evaluated for its ability to prevent muscle yield. 2. Three basic stimulus patterns, recruitment, step increases in stimulus rate, and doublets were imposed during the course of ramp stretches applied over a wide range of velocities. The effect of each stimulus pattern on muscle force was compared to the force output recorded without stretch-related recruitment or rate modulation. 3. Motor-unit recruitment was found to be most effective in preventing yield during muscle stretch. Newly recruited motor units showed no evidence of yielding for some 250 ms following activation, at which time muscle stiffness declined slightly. This time-dependent resistance to yield was observed regardless of whether the onset of the neural stimulus closely preceded or followed stretch onset. 4. Step increases in stimulus rate arising shortly after stretch onset did not prevent the occurrence of yield at most stretch velocities, but did augment muscle stiffness later in the stretch. Doublets in the stimulus train were found to augment muscle stiffness only when they occurred in newly recruited motor units. 5. These results suggest that at low or moderate initial forces, the prevention of yield in lengthening, reflexively intact muscle results primarily from rapid motor-unit recruitment. To a lesser extent, the spring-like character of the stretch-reflex response also derives from step increases in firing rate of motor units active before stretch onset and doublets in units recruited during the course of stretch. Smooth rate increases appear to augment muscle force later in the course of the reflex response.

Animals↗

Endoscopic findings in sarcoidosis. Characteristics and correlations with radiographic staging and bronchial mucosal biopsy yield.

Flexible fiberoptic bronchoscopy was performed in 101 patients with sarcoidosis, and the endoscopic findings were analyzed and correlated with radiographic stages and diagnostic bronchial biopsy. Endoscopic findings included bronchostenosis (26% of patients), mucosal nodularity (64%), hypervascularity (38%), and mucosal edema (55%). The only correlation between these findings and the radiographic stage was the presence of mucosal nodularity observed in 73% of stage I (14/19 patients), decreasing to only 48% in stage III (15/31 patients). Bronchial biopsy yield was 58% for stage I, 62% for stage II, and 46% for stage III, with overall yield of 57%. However, the addition of bronchial biopsies increased the diagnostic yield of the bronchoscopic procedure from 73% for transbronchial biopsies alone to 88% when the two procedures were combined. In the presence of endobronchial stenosis, mucosal nodularity and hypervascularity, bronchial biopsy yield was 91% vs 37% when these were absent. We conclude that the endoscopic characteristics, with the exception of nodularity, do not correlate with radiographic stages. Bronchial biopsy yield is higher in those patients with mucosal nodularity, increased vascularity, and bronchostenosis. Bronchial mucosal biopsy also improved the overall diagnostic yield when it is obtained in conjunction with transbronchial lung biopsy.

Biopsy↗

Estimation of yields of OH radicals in water irradiated by ionizing radiation.

The yield of OH radical induced by ionizing radiation was estimated by an empirical model; a prescribed diffusion model for a spur of single size applying to neutral water. Two representative spur distances were introduced, one for an incident primary charged particle and one for a representative secondary electron, to calculate chemical yields among active species in a spur. The total yield from the track was a combination of these primary and secondary yields. Two coefficients of this combination were the parameters of the present model. Based on an optimization of these parameters by existing experimental Fricke G-values, the present model estimates the yields of OH at the microsecond timescale after an irradiation, in a unified manner from electrons to heavy ions. The predicted yields of OH around the nanosecond timescale after an irradiation may be a relevant basis for a study on the mechanisms of radiation effects. This prediction by the present model was exemplified for electrons, photons and heavy ions (proton, helium, carbon, neon, argon and iron).

Computer Simulation↗

[The influence of anticancer agents at various stages to induce LAK cell on its cytotoxicity and cell yield. Examination in vitro].

To establish the combination of chemotherapy with adoptive immunotherapy (AIT), using lymphokine activated killer cells (LAK) and/or interleukin-2 (IL-2), author examined the following: 1) Pretreatment with anticancer agents for peripheral blood mononuclear cells (PBMC) and its effect on LAK cell cytotoxicity and cell yield. 2) The addition of anticancer agents in the induction phase to LAK cell and its effect on LAK cell cytotoxicity and cell yield. 3) Pretreatment with anticancer agents to induced LAK cell and its effect on LAK cell cytotoxicity and cell yield. 4) The cytotoxicity of LAK cell against tumor cells treated with anticancer agents. Our experiment has shown that when we take peripheral blood mononuclear cells (PBMC) to induce LAK cells, there is no influence on its LAK cell yield or its cytotoxicity after being harvested as LAK cells, even if there is a maximum concentration of anticancer agents, but in the case of the LAK cell induction phase VDS, CDDP, ADM and MMC have a significant effect on LAK cell yield and on cytotoxicity of LAK cell after being harvested as LAK cells. And it has also been shown that, if there is a maximum concentration of anticancer agents, it has an effect on the induced LAK cell cytotoxicity and on the LAK cell yield after being recultured with IL-2. On the other hand, LAK cell cytotoxicity makes no difference to tumor cells whether they are treated or not with anticancer agents. These results suggest that we can take peripheral blood mononuclear cells to induce LAK cells unrelated to the administration of anticancer agents, and that if we use a combination of chemotherapy with adoptive immunotherapy (IL-2 administration and/or LAK cell adaptation), we should start with the administration of anticancer agents and then administer IL-2 and/or transfer LAK cells after the concentration of anticancer agents decreased under 1/10 of maximum concentration in the blood level of our conventionally clinical use.

Antineoplastic Agents↗

Association of carcinoma yield with early papilloma development in SENCAR mice.

The responsiveness of SENCAR mouse skin to 20 different chemicals with known carcinogenic properties was assessed in initiation/promotion experiments. The purpose of these experiments was to evaluate the extent of false negative responses in mouse skin initiation/promotion protocols and to determine the extent to which early papilloma development can be used to predict the eventual development of malignant tumors. The chemicals were administered as initiators by four different routes: oral, intraperitoneal, subcutaneous, and topical. Following the initiating dose of carcinogen, the animals were subjected to topical applications of 1 microgram 12-O-tetradecanoylphorbol-13-acetate (TPA) 3 times per week for a period of 20 weeks. The yield of papillomas at 24 weeks was selected as a potential predictor of carcinoma yields at 52 weeks following the start of the promotion schedule. Positive responses were observed with only eight of the compounds tested. Where positive results were observed, there was some evidence that the response could depend both qualitatively and quantitatively on the route of administration. However, no route was clearly superior, i.e., different chemicals gave greater responses by different routes. Papilloma yield at 24 weeks following the start of the promotion schedule was clearly related to the development of carcinomas at 52 weeks. No simple linear relationship existed between papilloma yield and carcinoma development, since the number of malignant tumors per papilloma decreased with increasing papilloma yields. The relationship between papilloma and carcinoma yields appeared to be independent of the carcinogen used. These data indicate that there are some limitations in using mouse skin initiation/promotion experiments as the sole basis for identifying substances with carcinogenic activity.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Optimization of yield in kinetically controlled synthesis of ampicillin with immobilized penicillin acylase in organic media.

Immobilized penicillin acylase is a moderately priced versatile enzyme, that is able to catalyze the synthesis of derived penicillins and cephalosporins from the corresponding beta-lactam nuclei and proper side-chain precursors. Kinetically controlled synthesis is a better strategy when product yield is a key issue. Yield should increase at reduced water activity by depressing the competing hydrolytic reactions in favor of synthesis; therefore, organic cosolvents can be a suitable reaction media for synthesis. Using response surface methodology and product yield as objective function, temperature and pH were optimized in the kinetically controlled synthesis of ampicillin using previously screened cosolvents and reaction conditions. Optimum pH was 6.0 for ethylene glycol (EG) and glycerol (GL) and 6.6 for 1-2 propanediol (PD); optimum temperature was 30 degrees C for GL and for EG and PD was in the lower extreme of the range studied, optimum lying below 26 degrees C. Maximum molar yields predicted by the model were 58,51, and 46% for EG, GL, and PD, respectively, which were experimentally validated. Highest yield in aqueous buffer was always <40%. Molar yields about 60% compare favorably with values reported for the kinetically and thermodynamically controlled synthesis of ampicillin and other derived penicillins.

Ampicillin↗

Population rates of cardiac catheterization and yield of high-risk coronary artery disease.

BACKGROUND: The optimal population rate of cardiac catheterization is unknown. One potential way to determine it would be to examine whether there is a population rate beyond which the yield of high-risk coronary artery disease (CAD) does not rise. METHODS: Using a detailed clinical registry that captures all patients undergoing cardiac catheterization in Alberta, we determined annual population rates of cardiac catheterization and the corresponding yield of cases of high-risk CAD in each of Alberta's 17 health regions from 1995 to 2002. Least squares linear regression analysis and hierarchical modelling methods were then used to assess the linear relation between catheterization rates and rates of high-risk CAD. RESULTS: The age-adjusted average rate of cardiac catheterization among men ranged from 404.9 to 638.1 per 100,000 population aged over 20 years. Among women, the average rate ranged from 171.8 to 314.0 per 100,000. For both sexes, increased regional rates of catheterization were associated with a linearly increasing yield of high-risk CAD, with no evidence of a plateau in yield when more procedures were performed. One additional case of high-risk CAD was identified for every 2.5 additional cardiac catheterization procedures performed among men, and for every 3.7 additional procedures performed among women. INTERPRETATION: The increasing yield of patients with high-risk CAD associated with increased regional population rates of cardiac catheterization, together with the absence of a plateau in yield, suggests that Alberta's population rates of cardiac catheterization are suboptimal to detect people with high-risk CAD.

Adult↗

Relationship between cereal leaf beetle (Coleoptera: Chrysomelidae) egg and fourth-instar populations and impact of fourth-instar defoliation of winter wheat yields in North Carolina and Virginia.

Cereal leaf beetle, Oulema melanopus (L.), has become a serious pest of small grains in the mid-Atlantic region of the United States. Existing thresholds for implementing control measures allowed too much leaf damage and consequent yield loss to occur before recommending treatment. Information on beetle biology and crop response to injury, both prerequisites for developing new management strategies, was lacking for this region. A 3-yr project was initiated to generate an area wide cereal leaf beetle biological and yield impact database for winter wheat, and to evaluate the injury and yield loss potential of different population densities. Over the study period, beetle populations were evaluated at 26 winter wheat field locations in Virginia and North Carolina. Eggs and larvae, classified to instar, were counted twice each week from February to June. Replicated insecticide versus noninsecticide treatments were conducted at each location where leaf defoliation and yield were documented. Results showed that the relationship between 50th percentile egg and fourth-instar population estimates were in strong agreement (y = 0.36x - 0.01; r2 = 0.79). Potentially detrimental larval infestations were forecast before appearance of foliage injury from egg populations present during the stem elongation to flag leaf emergence developmental stages. A significant positive linear relationship between total fourth instar per stem population estimates and percent flag leaf defoliation was detected (y = 20.29x + 1.34; r2 = 0.60). A weaker but still significant relationship between the total fourth-instar population estimates and percent yield loss was found (y = 11.74x + 6.51; r2 = 0.26), indicating that factors in addition to flag leaf injury, primarily by fourth instars, also contributed to reduced yields.

Animals↗

Yields of tar, nicotine, and carbon monoxide in the sidestream smoke from 15 brands of Canadian cigarettes.

Sidestream smoke yields for 15 brands of cigarettes were determined under conditions where mainstream yields were approximately equal to those used for determining the values which appear on packages of Canadian cigarettes. Sidestream yields of tar, nicotine, and carbon monoxide were much higher than mainstream yields for all brands tested. The average sidestream-to-mainstream ratios for the 15 brands were 3.5, 6.6, and 6.8 for tar, nicotine, and carbon monoxide, respectively. The highest yields of sidestream were obtained from the brands with the lowest mainstream yields.

Canada↗

Consumption of 'low-yield' cigarettes: its frequency and relationship to serum thiocyanate.

To determine the use and possible health risks of low-yield cigarettes, we ascertained the cigarette brands and serum thiocyanate (SCN) levels of 2,561 adult smokers (age 25-74) in population-based samples of seven upper Midwestern communities during 1980-82. Brands were coded according to December 1981 Federal Trade Commission (FTC) ratings for "tar", nicotine, and carbon monoxide (CO). Compared to 1980 data from the National Center for Health Statistics for the United States as a whole, a greater proportion of smokers in these communities smoked low-yield brands. More people with higher education than lesser and more women than men smoked low-yield cigarettes. Greater proportions of older people (65-75 years) than younger people (less than 65 years) smoked cigarettes in the highest and lowest brand yield categories. SCN, adjusted for number of cigarettes smoked and for sex, was only weakly associated with brand ratings for "tar" (r = +.12), nicotine (R = +.11), and CO (r = +.15). Furthermore, the gradient in SCN between lowest and highest quintiles of brand strength was less than 16 per cent--much lower than the 300-500 per cent gradient in smoke components implied by FTC ratings. These data add to the evidence that smoking low-yield cigarettes may not be significantly less hazardous than smoking high-yield brands.

Adult↗

Waterlogging effects on growth and yield components in late-planted soybean.

A major agronomic problem in the southeastern USA is low yield of late-planted soybean [Glycine max (L.) Merr.]. This problem is aggravated by the adverse effect of waterlogging on crop growth. Our objectives were to identify soybean growth stages sensitive to waterlogging; identify yield components and physiological parameters explaining yield losses induced by waterlogging; and determine the extent of yield losses induced by waterlogging under natural field conditions. Greenhouse and field studies were conducted during 1993 and 1994 near Baton Rouge, LA, (30 degrees N Lat) on a Commerce silt loam. Waterlogging tolerance was assessed in cultivar Centennial (Maturity Group VI) at three vegetative and five reproductive growth stages by maintaining the water level at the soil surface in a greenhouse study. Using the same cultivar, we evaluated the effect of drainage in the field for late-planted soybean. Rain episodes determined the timing of waterlogging; redox potential and oxygen concentration of the soil were used to quantify the intensity of waterlogging stress. Results of the greenhouse study indicated that the early vegetative period (V2) and the early reproductive stages (R1, R3, and R5) were most sensitive to waterlogging. Three to 5 cm of rain per day falling on poorly drained soil was sufficient to reduce crop growth rate, resulting in a yield decline from 2453 to 1550 kg ha-1. Yield loss in both field and greenhouse studies was induced primarily by decreased pod production resulting from fewer pods per reproductive node. In conclusion, waterlogging was determined to be an important stress for late-planted soybean in high rainfall areas such as the Gulf Coast Region.

Agriculture↗

Relationship of maternal milk expected progeny differences to actual milk yield and calf weaning weight.

Calf weaning weights and estimates of 205-d milk yield (n = 317) were obtained from 90 Polled Hereford (PH) and 80 Simmental (SM) cows to evaluate the relationship of these measures with maternal milk and total maternal EPD from national cattle evaluations. An inverse parabolic exponential function predicted 205-d milk yields for each cow from five estimates of daily milk yield taken once per month via calf weigh-suckle-weigh. Changes in calves' 205-d adjusted weights were greater than predicted (P < .02) by maternal milk EPD of PH and SM dams and PH maternal grandsires (MGS) but were similar to changes predicted by SM MGS (P > .50). These changes were also greater than those predicted by total maternal EPD of PH dams and MGS (P < .02) but were similar to those predicted in SM dams and MGS (P > .38). Interactions of maternal milk and total maternal EPD with sex of calf and year did not influence calves' 205-d adjusted weights (P > .10). A positive relationship was found in both breeds between 205-d milk yield EPD (calculated from 205-d milk yield estimates) and maternal milk EPD from national cattle evaluations. Objectively assessing the magnitude of this relationship was hindered by problems in estimating heritability and interpreting correlations between EPD. These data indicate that maternal milk and total maternal EPD from national cattle evaluations are reasonably good predictors of genetic differences in milk yield and weaning weight.

Age Factors↗

Subprimal purchasing and merchandising decisions for pork: relationship to retail yield and fabrication time.

Boxed pork was obtained to represent four different purchase specifications (different anatomical separation locations and[or] external fat trim levels) common in the pork industry to conduct a study of retail yields and labor requirements. Bone-in loins (n = 180), boneless loins (n = 94), and Boston butts (n = 148) were assigned randomly to fabrication styles within subprimals. When comparing cutting styles within subprimals, it was evident that cutting style affected percentage of retail yield and cutting time. When more bone-in cuts were prepared from bone-in loin subprimals, retail yields ranged from 92.80 +/- .61 to 95.28 +/- .45%, and processing times ranged from 222.57 +/- 10.13 to 318.99 +/- 7.85 s, from the four suppliers. When more boneless cuts were prepared from bone-in loin subprimals, retail yields ranged from 71.12 +/- 1.10 to 77.92 +/- .77% and processing times ranged from 453.49 +/- 8.95 to 631.09 +/- 15.04 s from the different loins. Comparing boneless to bone-in cuts from bone-in loins resulted in lower yields and required greater processing times. Significant variations in yields and times were found within cutting styles. These differences seemed to have been the result of variation in supplier fat trim level and anatomical separation (primarily scribe length).

Animals↗

Predicting the yield and composition of mature cow carcasses.

Cow carcasses (n = 60) were selected based on conformation and external fat to develop more current and useful prediction equations for estimating yield and composition. Adjusted preliminary yield grade was highly correlated to percentage of the carcass as fat (.91), percentage fat in the total lean (.89), and percentage fat in the lean trimmings (.88) of carcasses from non-grain-fed mature cows. Equations for predicting percentage of the carcass as chemical fat had higher -R2 values than equations predicting other compositional end points. The "best" regression equation for predicting total yield (i.e., whole muscle cuts plus lean trimmings adjusted to 10% chemical fat) included hot carcass weight (HCWT), adjusted preliminary yield grade (APYG), longissimus area (LMA), and marbling (MARB), with R2 = .75 and residual standard deviation (RSD) = 2.47. A similar equation predicting total yield from unribbed carcass data included HCWT, APYG, and conformation (CONF) with R2 = .69 and RSD = 3.11. These two equations were applied to a test group of cow carcasses (n = 20), and the average difference between the actual and predicted total yield values from ribbed data and unribbed data was .45 and .83% of HCWT; simple correlations between the actual and predicted values were .74 and .69, respectively. These equations contain relatively simple independent variables to identify and more nearly represent current industry processing practices than equations previously available.

Aging↗

Influence of body condition score on by-product yield and value from cull beef cows.

Mature beef cows (n = 122) representing British and Continental phenotypes were slaughtered to measure the influence of body condition score (BCS) on by-product yield and value. All cows were weighed and assigned BCS, based on a 9-point scale, 24 h before slaughter. By-product weights were obtained during the slaughter process and included blood, feet (with hooves attached), oxlips, tongue, gullet, trachea, cheek meat, head meat, skull, tripe, honeycomb tripe, large and small intestines, spleen, mesenteric fat, weasand meat, kidneys, heart, lungs, and oxtail. By-product yields were calculated as a percentage of the animal's live weight taken 24 h before slaughter. By-product values were computed by multiplying the weight of each piece removed during the slaughter process by the 1997 average price. Live weight increased linearly (P<.001) as BCS increased from 2 to 8, whereas Continental cows were approximately 86 kg heavier (P<.05) at slaughter than British cows. Cows assigned a BCS of 2 or 3 had greater (P<.05) skull, feet, tongue, tripe, honeycomb tripe, trachea, and lung yields than cows assigned a BCS of 4 or higher. On the other hand, BCS-7 and 8 cows had greater (P<.05) weights and yields of large intestines and mesenteric fat than cows given a BCS of 6 or lower. The feet, trachea, lungs, and bone meal from BCS-2 cows had the greatest (P<.05) value, whereas the value of the large intestine, oxtail, and mesenteric fat was highest (P<.05) for BCS-7 and 8 cows. Weight, yield, and value of the skull, head meat, and feet were greater (P<.05) for Continental cows than British cows. Total by-product value was quadratically (P<.001) related to BCS. Cows assigned a BCS of 5 had lower (P<.05) total by-product values than either BCS-2 or BCS-7 and 8 cows. Drop credit for BCS-2 cows was greater (P<.05) than BCS-3, 4, 5, and 6 cows, with cows assigned a BCS of 7 and 8 having intermediate drop credit values. Continental cows tended to have greater (P<.10) total by-product and drop credit values than British cows. Information from this study indicated that the BCS of cows at the time of slaughter had a profound influence on by-product yields and, more importantly, values of by-products that are credited back against the cost of production to the beef cattle producer.

Animal Husbandry↗