[Ossification mechanisms in normal and pathological conditions].
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On the basis of radiographic studies on animals (rabbits), the authors determined the beginning of bone and dental hard tissue calcification. First signs of ossification were observed on the 17th day after copulation, whereas the calcification of the incisors began only on the 19th day after copulation.
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Forty-five caries-free primary molars from children with continuous fluoride supplementation (n = 28) after birth and from children without fluoride prophylaxis (n = 17) were investigated by light microscopy. The following results were obtained: The prophylaxis group evidenced a special form of calcification consisting of fibrodentin-like hard tissue developing intramurally in the dentin wall especially at the pulp floor and spreading irregularily into the pulp cavity by displacing the degenerating pulp tissue (n = 24). These teeth were ankylosed in the bi- and trifurcation area as well as inside the roots. This kind of hard tissue was not observed in the teeth from untreated children. Only 5 children had greater calcifications attached at the pulp wall. Nevertheless, this difference was statistically significant (p = 0.001) and indicates a relationship between fluoride prophylaxis, the special form of pulp calcification and tooth ankylosis.
Mineral particle clusters, corresponding to calcospherites or matrix vesicle-initiated clusters in calcifying cartilage and dentin and to collagen bundle-related mineralization in lamellar bone, have been isolated from NaOCl solutions used to dissolve the organic matrix in preparation of anorganic mineralizing fronts for scanning electron microscopy.
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