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Monoclonal antibodies to proteins from cocksfoot grass (Dactylis glomerata) pollen: isolation and N-terminal sequence of a major allergen.

Monoclonal antibodies (Mabs) raised to an aqueous extract of cocksfoot grass (Dactylis glomerata) pollen have been characterised. Mab 1B9 was demonstrated by SDS-PAGE and Western blotting to recognise a major allergen of an approximate molecular weight of 28 kD in this extract, termed DG3, and a component with a molecular weight of between 35 and 40 kD in an extract of Secale cereale (cultivated rye) pollen. The 28 kD component of cocksfoot grass pollen isolated by affinity chromatography using Mab 1B9 was recognised by IgE antibodies in 80% (8 of 10) atopic sera, but only weakly by 25% (1 of 4) non-atopic sera tested. N-terminal sequencing of DG3 purified by affinity chromatography, 2 D electrophoresis and electroblotting to polyvinylidenedifluoride revealed significant homology with a group-V allergen (Phl p V) from timothy grass (Phleum pratense).

Amino Acid Sequence↗

Genomic in situ hybridization to sectioned nuclei shows chromosome domains in grass hybrids.

In situ hybridization using biotinylated total genomic DNA and avidin detection systems was adapted for examination of thin-sectioned plant material in the light and electron microscopes. Root tip material was preserved prior to sectioning, so that the in vivo disposition of the chromatin was maintained. Use of total genomic DNA from Secale africanum as a probe enabled the chromatin from the two parental genomes in the grass hybrid Hordeum chilense x S. africanum to be distinguished. The biotinylated probe preferentially labelled the chromosomes of S. africanum origin. DNA-DNA hybrids were visualized at the light-microscope level by Texas Red fluorescence and at the electron-microscope level by the enzymic precipitation of DAB (diaminobenzidine) or by colloidal gold particles. The use of thin sections allowed the location of probe hybridization to be established unequivocally in both metaphase and interphase nuclei. Analysis of interphase nuclei showed that chromatin originating from the two parental genomes did not intermix but occupied distinct domains.

Chromatin↗

Production of a monoclonal antibody specific for high molecular weight glutenin subunits (HMW-GS) in wheat and its antigenic determinant.

Wheat high molecular weight glutenin subunits (HMW-GS) 1Bx14 and 1By15 isolated by preparative SDS-PAGE are used as antigen to immunize BALB/c mice. Subcutaneous inoculation of the antigen is performed. The intra-peritoneal injection is completed 3 days before fusion with myeloma cell (SP2/0) via PEG-1500. The fusion cells are selected by indirect enzyme-linked immuno-sorbent assay (ELISA). Positive hybrid cells are further verified three times by limit dilution of the culture cells. A hybridoma cell line is successfully obtained. The monoclonal antibody belongs to IgG1 subclass. In immunoblotting, the antibody binds to all HMW-GS of T. aestivum cultivars, but does not bind to other storage proteins in seeds of wheat. This result is consisting with the high homology in amino acid sequences among the HMW glutenin subunits in wheat. The antibody also binds to HMW-GS storage proteins in Aegilops squarrosa and T. durum (durum wheat). Furthermore, it also binds to HMW storage proteins in Secale cereale (rye), Hordeum vulgare (barley). However, it never binds seed storage proteins in other cereals such as maize, oat, rice, foxtail millet, sorghum etc. The antigen determinant recognized by the antibody has been located within hexapeptide [PGQGQQ] or / and nonapeptide [GYYPTSPQQ] in the central repetitive region of HMW-GS.

Alleles↗

Comparison of four grass pollen species concerning their allergens of grass group V by 2D immunoblotting and microsequencing.

The identification and characterization of allergenic components is a vital step towards improving diagnosis and therapy. Members of the grass family (Poaceae) reveal a high cross-reactivity among each other caused by the close phylogenetical relationship. In order to investigate the variability between allergenic components, we studied the allergen grass group V, one of the major allergens. Pollen extracts of 4 different tribes (timothy grass (Phleum pratense)--Agrostidae, perennial rye grass (Lolium perenne)--Festuceae, meadow velvet (Holcus lanatus)--Aveneae, and rye (Secale cereale)--Triticeae) of the Festucoideae subfamily were separated by 2D PAGE and investigated by immunoblotting using patients' poolserum and monoclonal antibodies (raised against group V allergens of timothy grass pollen). The antibodies identify different allergens in the four grass species. The components vary from 30-50 kDa and pI 4.8-7.0. The eight NH2-terminal amino acids were determined and indicated high similarities between the different components. These results cast doubt on the suitability of classifying allergens into groups based only on their molecular mass, isoelectric point and N-terminal sequence analysis. It suggests to classify allergens according to their IgE-reactive epitopes.

Allergens↗

Construction and evaluation of cDNA libraries for large-scale expressed sequence tag sequencing in wheat (Triticum aestivum L.).

A total of 37 original cDNA libraries and 9 derivative libraries enriched for rare sequences were produced from Chinese Spring wheat (Triticum aestivum L.), five other hexaploid wheat genotypes (Cheyenne, Brevor, TAM W101, BH1146, Butte 86), tetraploid durum wheat (T. turgidum L.), diploid wheat (T. monococcum L.), and two other diploid members of the grass tribe Triticeae (Aegilops speltoides Tausch and Secale cereale L.). The emphasis in the choice of plant materials for library construction was reproductive development subjected to environmental factors that ultimately affect grain quality and yield, but roots and other tissues were also included. Partial cDNA expressed sequence tags (ESTs) were examined by various measures to assess the quality of these libraries. All ESTs were processed to remove cloning system sequences and contaminants and then assembled using CAP3. Following these processing steps, this assembly yielded 101,107 sequences derived from 89,043 clones, which defined 16,740 contigs and 33,213 singletons, a total of 49,953 "unigenes." Analysis of the distribution of these unigenes among the libraries led to the conclusion that the enrichment methods were effective in reducing the most abundant unigenes and to the observation that the most diverse libraries were from tissues exposed to environmental stresses including heat, drought, salinity, or low temperature.

Expressed Sequence Tags↗

Influence of cover crops on insect pests and predators in conservation tillage cotton.

In fall 2000, an on-farm sustainable agricultural research project was established for cotton, Gossypium hirsutum L., in Tift County, Georgia. The objective of our 2-yr research project was to determine the impact of several cover crops on pest and predator insects in cotton. The five cover crop treatments included 1) cereal rye, Secale cereale L., a standard grass cover crop; 2) crimson clover, Trifolium incarnatum L., a standard legume cover crop; 3) a legume mixture of balansa clover, Trifolium michelianum Savi; crimson clover; and hairy vetch, Vicia villosa Roth; 4) a legume mixture + rye combination; and 5) no cover crop in conventionally tilled fields. Three main groups or species of pests were collected in cover crops and cotton: 1) the heliothines Heliothis virescens (F.) and Helicoverpa zea (Boddie); 2) the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois); and 3) stink bugs. The main stink bugs collected were the southern green stink bug, Nezara viridula (L.); the brown stink bug, Euschistus servus (Say); and the green stink bug, Acrosternum hilare (Say). Cotton aphids, Aphis gossypii Glover, were collected only on cotton. For both years of the study, the heliothines were the only pests that exceeded their economic threshold in cotton, and the number of times this threshold was exceeded in cotton was higher in control cotton than in crimson clover and rye cotton. Heliothine predators and aphidophagous lady beetles occurred in cover crops and cotton during both years of the experiment. Geocoris punctipes (Say), Orius insidiosus (Say), and red imported fire ant, Solenopsis invicta Buren were relatively the most abundant heliothine predators observed. Lady beetles included the convergent lady beetle, Hippodamia convergens Guérin-Méneville; the sevenspotted lady beetle, Coccinella septempunctata L.; spotted lady beetle, Coleomegilla maculata (DeGeer); and the multicolored Asian lady beetle, Harmonia axyridis (Pallas). Density of G. punctipes was higher in cotton fields previously planted in crimson clover compared with control cotton fields for all combined sampling dates in 2001. Intercropping cotton in live strips of cover crop was probably responsible for the relay of G. punctipes onto cotton in these crimson clover fields. Density of O. insidiosus was not significantly different between cover crop and control cotton fields. Lady beetles seemed to relay from cover crops into cotton. Conservation of the habitat of fire ants during planting probably was responsible for the higher density of red imported fire ants observed in all conservation tillage cotton fields relative to control cotton fields. Reduction in the number of times in which economic thresholds for heliothines were exceeded in crimson clover and rye compared with control fields indicated that the buildup of predaceous fire ants and G. punctipes in these cover crops subsequently resulted in reduction in the level of heliothines in conservation tillage cotton with these cover crops compared with conventional tillage cotton without cover crops.

Agriculture↗

Categories and inheritance of resistance to Russian wheat aphid (Homoptera: Aphididae) biotype 2 in a selection from wheat cereal introduction 2401.

The Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Homoptera: Aphididae), is one of the most devastating insect pests of wheat (Triticum spp.) and barley (Hordeum spp.) in the world. Yield losses and control costs are valued at several hundred million dollars each year. The use of D. noxia-resistant cultivars is an ecologically, economically, and biologically sound method of managing this pest. Several D. noxia resistance (Dn) genes from wheat have been used to develop cultivars resistant to D. noxia. However, a new U.S. D. noxia biotype (biotype 2) in Colorado is virulent to all known Dn genes except the Dn7 gene from rye (Secale spp.). Hence, there is an immediate need to identify and characterize unique sources of D. noxia resistance to biotypes. In this article, we report resistance to D. noxia biotype 2, identified in a selection from wheat cereal introduction (CItr) 2401, that is controlled by two dominant genes. CItr2401 has a strong antibiosis effect that is exhibited as a reduced intrinsic rate of increase of D. noxia biotype 2. CItr2401 plants also exhibit tolerance to leaf rolling and chlorosis. No antixenosis was detected in CItr2401.

Animals↗

Influence of instar and commodity on insecticidal effect of two diatomaceous earth formulations against larvae of Ephestia kuehniella (Lepidoptera: Pyralidae).

Laboratory experiments were carried out to evaluate the insecticidal effect of two diatomaceous earth (DE) formulations, SilicoSec and PyriSec, against larvae of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Three instars were tested: first, third and fifth. The test was conducted in six commodities: barley Hordeum vulgare (L.) (Gramminae), rye Secale cereale L. (Gramminae), wheat Triticum sp. (Gramminae), wheat + 10% cracked wheat, wheat + 30% cracked wheat, and wheat flour. Quantities of these commodities were treated with the DEs at three dose rates: 250, 500, and 1000 ppm. Mortality of the exposed larvae on the DE-treated commodities was measured after 7 d of exposure. For both Des, mortality increased with dose, but this increase was lower when dose was increased from 500 to 1,000 ppm. The type of the commodity significantly affected DE effectiveness. Both DEs were equally effective on barley, rye, and wheat, whereas the presence of cracked wheat reduced larval mortality. In addition, significantly fewer larvae were dead on treated flour compared with the other five commodities. The increase of larval age significantly affected DE effectiveness. First instars were very susceptible to both DEs, and mortality with 1,000 ppm exceeded 86%. In contrast, fifth instar were the least susceptible to DEs, because mortality with 1000 ppm was < 22%.

Animals↗

Nitrogen removal and nitrate leaching for forage systems receiving dairy effluent.

Florida dairies need year-round forage systems that prevent loss of N to ground water from waste effluent sprayfields. Our purpose was to quantify forage N removal and monitor nitrate N (NO3(-)-N) concentrations in soil water below the rooting zone for two forage systems during four 12-mo cycles (1996-2000). Soil in the sprayfield is an excessively drained Kershaw sand (thermic, uncoated Typic Quartzipsamment). Over four cycles, average loading rates of effluent N were 500, 690, and 910 kg ha(-1) per cycle. Nitrogen removed by the bermudagrass (Cynodon spp.)-rye (Secale cereale L.) system (BR) during the first three cycles was 465 kg ha(-1) per cycle for the low loading rate, 528 kg ha(-1) for the medium rate, and 585 kg ha(-1) for the high. For the corn (Zea mays L.)-forage sorghum [Sorghum bicolor (L.) Moench]-rye system (CSR), N removals were 320 kg ha(-1) per cycle for the low rate, 327 kg ha(-1) for the medium, and 378 kg ha(-1) for the high. The higher N removals for BR were attributed to higher N concentration in bermudagrass (18.1-24.2 g kg(-1)) than in corn and forage sorghum (10.3-14.7 g kg(-1)). Dry matter yield declined in the fourth cycle for bermudagrass but N removal continued to be higher for BR than CSR. The BR system was much more effective at preventing NO3(-)-N leaching. For CSR, NO3(-)-N levels in soil water (1.5 m below surface) increased steeply during the period between the harvest of one forage and canopy dosure of the next. Overall, the BR system was better than CSR at removing N from the soil and maintaining low NO3(-)-N concentrations below the rooting zone.

Animals↗

Enzyme activities and arylsulfatase protein content of dust and the soil source: biochemical fingerprints?

Little is known about the potential of enzyme activities, which are sensitive to soil properties and management, for the characterization of dust properties. Enzyme activities may be among the dust properties key to identifying the soil source of dust. We generated dust (27 and 7 microm) under controlled laboratory conditions from agricultural soils (0-5 cm) with history of continuous cotton (Gossypium hirsutum L.) or cotton rotated with peanut (Arachis hypogaea L.), sorghum [Sorghum bicolor (L.) Moench], rye (Secale cereale L.), or wheat (Triticum aestivum L.) under different water management (irrigated or dryland) and tillage (conservation or conventional) systems. The 27- and 7-microm dust samples showed activities of beta-glucosidase, alkaline phosphatase, and arylsulfatase, which are related to cellulose degradation and phosphorus and sulfur mineralization in soil, respectively. Dust samples generated from a loam and sandy clay loam showed higher enzyme activities compared with dust samples from a fine sandy loam. Enzyme activities of dust samples were significantly correlated to the activities of the soil source with r > 0.74 (P < 0.01). The arylsulfatase proteins contents of the soils (0.04-0.65 mg protein kg(-1) soil) were lower than values reported for soils from other regions, but still dust contained arylsulfatase protein. The three enzyme activities studied, as a group, separated the dust samples due to the crop rotation or tillage practice history of the soil source. The results indicated that the enzyme activities of dust will aid in providing better characterization of dust properties and expanding our understanding of soil and air quality impacts related to wind erosion.

Agriculture↗

Optimizing organic fertilizer applications under steady-state conditions.

Because organic N fertilizers must be mineralized before they become plant-available, application designs should consider time and temperature effects on N release as well as crop N requirements. This study presents deterministic (DOpt) and stochastic (SOpt) linear optimization models to determine sustainable land application schedules. The easily solved models minimize the amount of N that is applied while assuring than crop N demands are met as they develop. Temperature effects on N mineralization were included by using the Arrhenius equation to create a temperature-adjusted time series. Uncertainties associated with mineralization rates and the temperature-adjustment (Q10) factor are considered by SOpt. Examples are presented for a summer maize (Zea mays L.) and winter triticale (Triticum aestivum L. x Secale cereale L.) rotation operated by a hypothetical dairy operation in Stanislaus County, California. Monte Carlo simulations were used to test the models. A closed-form solution for estimating the time until steady state is presented and steady-state conditions were reached within 7 yr after applications were initiated. Because of temperature effects, DOpt solutions were 12% greater during the winter and 29% lower during the summer than a reference approach that applied liquid manure at 130% of the crop N demand. Stochastic linear optimization values were 1.7% greater than DOpt values in the summer and 6.2% greater in the winter. Surplus N estimates from Monte Carlo simulations averaged 104 kg ha(-1) for DOpt and 126 ka ha(-1) for SOpt, but SOpt was much less likely to result in crop N deficits. Linear optimization is a viable tool for scheduling organic N applications.

Agriculture↗

Nitrous oxide emissions from corn-soybean systems in the midwest.

Soil N2O emissions from three corn (Zea mays L.)-soybean [Glycine max (L.) Merr.] systems in central Iowa were measured from the spring of 2003 through February 2005. The three managements systems evaluated were full-width tillage (fall chisel plow, spring disk), no-till, and no-till with a rye (Secale cereale L. 'Rymin') winter cover crop. Four replicate plots of each treatment were established within each crop of the rotation and both crops were present in each of the two growing seasons. Nitrous oxide fluxes were measured weekly during the periods of April through October, biweekly during March and November, and monthly in December, January, and February. Two polyvinyl chloride rings (30-cm diameter) were installed in each plot (in and between plant rows) and were used to support soil chambers during the gas flux measurements. Flux measurements were performed by placing vented chambers on the rings and collecting gas samples 0, 15, 30, and 45 min following chamber deployment. Nitrous oxide fluxes were computed from the change in N2O concentration with time, after accounting for diffusional constraints. We observed no significant tillage or cover crop effects on N2O flux in either year. In 2003 mean N2O fluxes were 2.7, 2.2, and 2.3 kg N2O-N ha(-1) yr(-1) from the soybean plots under chisel plow, no-till, and no-till + cover crop, respectively. Emissions from the chisel plow, no-till, and no-till + cover crop plots planted to corn averaged 10.2, 7.9, and 7.6 kg N2O-N ha(-1) yr(-1), respectively. In 2004 fluxes from both crops were higher than in 2003, but fluxes did not differ among the management systems. Fluxes from the corn plots were significantly higher than from the soybean plots in both years. Comparison of our results with estimates calculated using the Intergovernmental Panel on Climate Change default emission factor of 0.0125 indicate that the estimated fluxes underestimate measured emissions by a factor of 3 at our sites.

Climate↗

Carbon supply and storage in tilled and nontilled soils as influenced by cover crops and nitrogen fertilization.

Soil carbon (C) sequestration in tilled and nontilled areas can be influenced by crop management practices due to differences in plant C inputs and their rate of mineralization. We examined the influence of four cover crops {legume [hairy vetch (Vicia villosa Roth)], nonlegume [rye (Secale cereale L.)], biculture of legume and nonlegume (vetch and rye), and no cover crops (or winter weeds)} and three nitrogen (N) fertilization rates (0, 60 to 65, and 120 to 130 kg N ha(-1)) on C inputs from cover crops, cotton (Gossypium hirsutum L.), and sorghum [Sorghum bicolor (L.) Moench)], and soil organic carbon (SOC) at the 0- to 120-cm depth in tilled and nontilled areas. A field experiment was conducted on Dothan sandy loam (fine-loamy, siliceous, thermic Plinthic Paleudults) from 1999 to 2002 in central Georgia. Total C inputs to the soil from cover crops, cotton, and sorghum from 2000 to 2002 ranged from 6.8 to 22.8 Mg ha(-1). The SOC at 0 to 10 cm fluctuated with C input from October 1999 to November 2002 and was greater from cover crops than from weeds in no-tilled plots. In contrast, SOC values at 10 to 30 cm in no-tilled and at 0 to 60 cm in chisel-tilled plots were greater for biculture than for weeds. As a result, C at 0 to 30 cm was sequestered at rates of 267, 33, -133, and -967 kg C ha(-1) yr(-1) for biculture, rye, vetch, and weeds, respectively, in the no-tilled plot. In strip-tilled and chisel-tilled plots, SOC at 0 to 30 cm decreased at rates of 233 to 1233 kg C ha(-1) yr(-1). The SOC at 0 to 30 cm increased more in cover crops with 120 to 130 kg N ha(-1) yr(-1) than in weeds with 0 kg N ha(-1) yr(-1), regardless of tillage. In the subtropical humid region of the southeastern United States, cover crops and N fertilization can increase the amount of C input and storage in tilled and nontilled soils, and hairy vetch and rye biculture was more effective in sequestering C than monocultures or no cover crop.

Agriculture↗

Five year-round forage systems in a dairy effluent sprayfield: phosphorus removal.

In northern Florida, forages are grown in dairy effluent sprayfields to recover excess P. Our purpose was to evaluate five year-round forage systems for their capacity to remove P from a dairy sprayfield. The soil is a Kershaw sand (thermic, uncoated Typic Quartzipsamment). Systems included bermudagrass (Cynodon spp.)-rye (Secale cereale L.) (BR), perennial peanut (Arachis glabrata Benth.)-rye (PR), corn (Zea mays L.)-forage sorghum [Sorghum bicolor (L.) Moench]-rye (CSR), corn-bermudagrass-rye (CBR), and corn-perennial peanut-rye (CPR). Forages were grown for five 12-mo cycles. Effluent P rates were 80, 120, and 165 kg ha-1 cycle-1. The 5-cycle P removal was 67 kg ha-1 cycle-1 for BR, 54 kg ha-1 for CBR, 52 kg for CSR, 45 kg for PR, and 43 for CPR. Removal of P by winter rye was low. There were differences in system rankings among cycles primarily due to changes in the performance of perennial forages. In the first two cycles, BR had the greatest P removal (91 kg ha-1 cycle-1) due to high bermudagrass yield and P concentration. In the first cycle, P removal was lowest for PR (36 kg ha-1) because perennial peanut was slow to establish. In later cycles, P removal for BR declined because bermudagrass yield and P concentration declined. It increased for PR because peanut yield increased. The yield of corn in CBR, CPR, and CSR was consistently high but P concentration was modest (avg. 2.2 g kg-1). Sorghum produced moderate but stable yield and had low P levels (avg. 1.8 g kg-1). Effluent rate marginally affected the performance of most grasses. For P recovery in dairy sprayfields in northern Florida, the best warm-season forage would likely be a high yielding, persistent bermudagrass.

Animal Feed↗

Technical note: stocking equivalents and stocking rate-gain relationships for steers and cow-calf pairs grazing oversown bermuda grass.

Grazing experiments may use steers or cow-calf pairs for measuring animal performance on pasture treatments, but the validity of extrapolation between these classes of cattle has not been verified. A grazing study was conducted in the spring and summer of both 1988 and 1989 to determine stocking equivalents and stocking rate-weight gain relationships for steers and cow-calf pairs grazing Coastal bermuda grass (Cynodon dactylon [L.] pers.) oversown with rye (Secale cereale L.) and ryegrass (Lolium multiflorum Lam.). Average daily gain and stocking rate (SR; 3.2, 4.2, 6.2, and 7.4 animals per hectare for steers and 1.7, 2.5, 3.7, and 4.9 pairs per hectare for cow-calf pairs) were both adjusted so that comparisons could be made on an equal BW basis. Disk meter height readings were used as measurements of forage accessibility. Disk meter height responses to SR did not differ (P greater than .10) between steer and cow-calf paddocks. There was a linear (P less than .001) decrease in ADG as SR increased, but this decline was steeper (P less than .001) for steers than for cows or suckling calves. Steers tended to be more productive than calves at low SR but less productive at high SR. Disk meter heights for the range of SR used in the study did not differ (P greater than .10) for steers and cow-calf pairs at equivalent BW per hectare. Our study suggests that live BW is a reasonable basis for determining forage requirements of steers and cow-calf pairs under grazing conditions, but extrapolation of production between classes of livestock will not be reliable.

Animal Feed↗

Forage systems for production of stocker steers in the upper south.

The southern states produce large numbers of beef calves that are generally weaned and sold in autumn. Keeping calves in this region beyond weaning to graze high-quality forages through a stocker cattle phase could improve profitability. Autumn-weaned Angus crossbred steers were allocated by breeding and weight to four forage systems that began in mid-November and continued through mid-October as follows: System 1, tall fescue (Festuca arundinacea Schreb.) and Kentucky bluegrass (Poa pratensis L.)-white clover (Trifolium repens L.); System 2, tall fescue, caucasian bluestem (Bothriochloa caucasica [Trin.] C. E. Hubbard) and tall fescue-red clover (Trifolium pratense L.); System 3, orchardgrass-alfalfa and bluegrass-white clover; and System 4, rye (Secale cereale L.), soybeans (Glycine max)-foxtail millet (Setaria italica), and bluegrass-white clover. All steers were supplemented with hay or silage previously cut from their respective systems when forage for grazing was limited. System 2 which used stockpiled tall fescue for winter grazing and caucasian bluestem for summer forage plus fescuered clover for hay and grazing in a three-paddock system, resulted in greater (P < .01) gain per hectare and per steer, more grazing days, and reduced stored forage requirements and produced more surplus feed than the other systems tested. Gains per hectare for Systems 1 through 4 were 454, 554, 472, and 487 kg (SE = 18), respectively. Harvested forage from Systems 1, 2, and 3 met needs for stored forages but System 4 required additional "purchased" hay. Stored forage was fed for 61, 38, 112, or 104 d for Systems 1 through 4, respectively. Within the physio-climatic region of this experiment, a simple three-paddock system based on cool- and warm-season perennial forages could improve beef production per unit of land area while reducing inputs of labor and equipment.

Animal Feed↗

Winter-annual pasture as a supplement for beef cows.

In each of two experiments, 120 pregnant beef cows were stratified by body condition score, BW, breed, and age, randomly divided into six groups of 20, and assigned to one of six 5.1-ha bermudagrass (Cynodon dactylon [L.] Pers.) pastures (two replicates/ treatment) in early January to evaluate the use of winter-annual pasture as a supplement. All cows in Exp. 1 and 2 had ad libitum access to bermudagrass/dallisgrass (Paspalum dilatatum Poir.) hay plus three treatments: 1) a concentrate-based supplement fed 3 d/wk, 2) limit grazing on winter-annual pasture 2 d/wk (7 hr/ d; 0.04 ha x cow(-1) x grazing d(-1)), or 3) limit grazing on winter-annual pasture 3 d/wk (7 hr/d; 0.04 ha x cow(-1) x grazing d(-1)) sod-seeded into a portion of the pasture until mid-May. The seeded portion of pastures in Exp. 1 was planted with a mixture of wheat (Triticum aestivum L.) and rye (Secale cereale L.), but annual ryegrass (Lolium multiflorum Lam.) was added to the seed mixture in Exp. 2. In mid-May, cows were blocked by treatment and the previous sorting factors, randomly assigned to six new groups of 20, and placed on the six perennial pastures until calves were weaned. Groups of cows were exposed to a bull for 60 d beginning in mid-May. In Exp. 1 and 2, limit-grazing winter-annual pasture compared to the concentrate-based supplement or limit grazing 2 vs 3 d/wk did not affect (P > 0.15) cow BW. In Exp. 1, cows limit grazed on winter-annual pasture had a lower (P = 0.05) body condition score than cows fed the concentrate-based supplement in the early spring. However, in Exp. 2, cows limit grazed on winter-annual pasture had higher (P < or = 0.07) body condition score than cows fed the concentrate-based supplement. The conception rate of cows in Exp. 1 and 2 did not differ (P > 0.22) between cows fed concentrate-based supplements and cows limit grazed on winter-annual pasture. In Exp. 2, cows limit grazed 2 d/wk tended to have a greater (P = 0.10) conception rate than cows limit grazed 3 d/wk. In Exp. 1 and 2, birth weight, total gain, BW, and ADG of calves were not affected (P > 0.15) by treatment. We conclude that wheat and rye pasture is a marginal supplement for lactating beef cows. However, cows limit grazed 2 d/wk on winter-annual pasture of wheat, rye, and annual ryegrass as a supplement maintained BW and body condition score as well as cows fed the concentrate-based supplement. But, grazing pasture 3 vs 2 d/wk did not seem to affect performance of cows.

Animal Feed↗

[Detection of alien chromatins introgressed into wheat in mitotic interphase] [In Process Citation]

Fluorescent in situ hybridization of biotin labelled total genomic DNA from alien species as probes was applied to illustrate chromatin behaviors of Secale cereale L., Thinopyrum intermedium Host., Avana fatua L., and Dasypyrum villosum (L.) Candagy introgressed into wheat (Triticum aestivum L.) in interphase cells. A pair of 1RS chromosome arms in 1BL/1RS translocations were visible as discrete domains traversing through the interphase nucleolus. The substitution and addition 1R chromosomes as well as the translocated chromosome segments of Th. intermedium and A. avena appeared as bright spots in interphase. In callli derived from immature embryos of a hybrid between T. durum-D. villosum amphidiploid and T. aestivum, seven D. villosum originated chromatins presented nearly consecutive trend from spots to domains. Chromatins from both parents did not tend to mix with each other at interphase of the hybrid calli. It is feasible to count the number of alien chromosomes and chromosome segments in the case of addition, substitution and/or translocation at interphase. As far as more alien chromosomes are considered, it seems not reliable to analyze the interphase nucleolus alone because some of the signals might be overlapped by chromatins of wheat. Neither S. cereale satellited chromosome 1R nor D. villosum 1V chromosome involving within the envelop of interphase nucleolus were associated with nucleoli, suggesting that their functions in the formation of nucleoli are inhibited in the wheat background.

Journal Article↗