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Hydrometer test for estimation of immunoglobulin concentration in bovine colostrum.

A practical field method for measuring immunoglobulin concentration in bovine colostrum has been developed from the linear relationship between colostral specific gravity and immunoglobulin concentration. Fourteen colostrums were collected within 24 h postpartum from nursed and unnursed cows and were assayed for specific gravity and major colostral constituents. Additionally, 15 colostrums were collected immediately postpartum prior to suckling and assayed for specific gravity and immunoglobulin concentration. Regression analysis provided an equation to estimate colostral immunoglobulin concentration from the specific gravity of fresh whole colostrum. From this, a colostrometer was developed for practical field use.

Animals↗

Effect of hydration on random levels of urinary pregnanediol glucuronide.

To determine the effect hydration has upon the relationship between serum progesterone and its urinary metabolite pregnanediol-3 alpha-glucuronide (PDG) we measured spot samples from patients (n = 207) presenting in the 1st trimester of pregnancy. Serum and urine specimens were obtained simultaneously and measured for progesterone and PDG by radioimmunoassay (RIA). Urine specific gravity was also measured at the time of sample collection. Results demonstrated that in samples where the urine specific gravity was greater than or equal to 1.015 there was a strong positive correlation between serum progesterone and PDG (r greater than 0.60, p less than 0.001). However, when urine samples were below a specific gravity of 1.015, serum and urinary steroid values correlated poorly or not at all. We conclude that the measurement of specific gravity should be routinely performed when determining random values of urinary PDG, since only samples in which the urine is adequately concentrated accurately reflect corresponding serum progesterone concentrations.

Adolescent↗

Method for preservation of water content during prolonged storage of cerebral tissue.

We set out to determine whether prolonged freezing of samples of cerebral tissue would alter specific gravity measurements. Our goal was to devise a method to prevent tissue water evaporation during transportation and storage of human cerebral samples in order to allow accurate measurements of specific gravity and water content at a delayed time. Control cerebral samples from healthy and water-intoxicated anaesthetized adult mice were immediately immersed in a gravimetric column for fresh specific gravity measurements. Cerebral samples from the same animals were also preserved by rapid freezing in liquid nitrogen, followed by deep-freeze storage at -80 degrees C for up to 7 days. The mean specific gravity and standard error of 65 non-oedematous and 18 oedematous fresh cerebral samples was 1,0483 +/- 2 x 10(-4) and 1,0433 +/- 5 x 10(-4), respectively. Freezing for 1 h to 7 days resulted in no significant alteration of specific gravity for 109 non-oedematous and 27 oedematous samples (P greater than 0.05). We describe a rapid freezing technique that is practical for transportation and storage of experimental or surgical cerebral specimens. Our findings indicate that storage of cerebral samples in liquid nitrogen, with or without subsequent deep-freezing, preserves water content of normal and oedematous cerebral tissue, thereby allowing accurate measurements at a later time.

Body Water↗

Urine concentration and enuresis in healthy preschool children.

OBJECTIVES: To determine whether children concentrate their urine overnight and to assess the correlation between specific gravity of a first morning urine specimen and the results of a questionnaire concerning bedwetting, voiding habits, and continence in 47 healthy children aged 3 to 6 years. DESIGN: A prospective observer-blinded consecutive sample. SETTING: Two San Francisco, Calif., preschools. PARTICIPANTS: Forty-seven children attending preschool during the study period. MEASUREMENTS/MAIN RESULTS: Forty of 47 children had a urine specific gravity greater than 1.020. None of these children wet the bed during this study, although four (11%) of 36 had a history of bedwetting. Furthermore, seven children with a urine specific gravity of 1.015 or lower had a history of bedwetting and wet the bed during this study. A voiding frequency of six or more times per day, by history, was associated with a 3:1 relative risk of bedwetting but did not segregate children with primary enuresis from those with secondary enuresis. CONCLUSIONS: Our results indicate that healthy children aged 3 to 6 years are able to concentrate their urine. In addition, urine specific gravity was an accurate predictor of the presence of nocturnal enuresis in this group of children. Our results suggest that a specific gravity of the first morning urine specimen should be correlated with appropriate history before extensive diagnostic evaluation or empiric therapy is performed in children with nocturnal enuresis.

Child↗

Fluid losses and hydration status of industrial workers under thermal stress working extended shifts.

AIMS: To assess whether workers under significant thermal stress necessarily dehydrated during their exposure and whether "involuntary dehydration" was inevitable, as supported by ISO 9866 and other authorities. Other objectives were to quantify sweat rates against recommended occupational limits, to develop a dehydration protocol to assist with managing heat exposures, and to understand the role of meal breaks on extended shifts in terms of fluid replacement. METHODS: A field investigation to examine the fluid consumption, sweat rates, and changes in the hydration state of industrial workers on extended (10, 12, and 12.5 hour) shifts under significant levels of thermal stress (wet bulb globe temperature (WBGT) >28 degrees C) was conducted on 39 male underground miners. Urinary specific gravity was measured before, during, and at the completion of the working shift. Environmental conditions were measured hourly during the shift. Fluid replacement was measured during the working periods and during the meal breaks. RESULTS: Average environmental conditions were severe (WBGT 30.9 degrees C (SD 2.0 degrees C), range 25.7-35.2 degrees C). Fluid intake averaged 0.8 l/h during exposure (SD 0.3 l/h, range 0.3-1.5 l/h). Average urinary specific gravity at start, mid, and end of shift was 1.0251, 1.0248, and 1.0254 respectively; the differences between start and mid shift, mid and end shift, and start and end shift were not significant. However, a majority of workers were coming to work in a moderately hypohydrated state (average urinary specific gravity 1.024 (SD 0.0059)). A combined dehydration and heat illness protocol was developed. Urinary specific gravity limits of 1.022 for start of shift and 1.030 for end of shift were selected; workers exceeding these values were not allowed into the workplace (if the start of shift limit was exceeded) or were retested prior to their next working shift (if the end of shift limit was exceeded). A target of 1.015 as a euhydrated state for start of shift was adopted for workforce education. CONCLUSIONS: This study found that "involuntary dehydration" did not occur in well informed workers, which has implications for heat stress standards that do not make provision for full fluid replacement during heat exposure. Fluid replacement during meal breaks was not significantly increased above fluid replacement rates during work time, with implications for the duration and spacing of meal breaks on long shifts. Testing of urinary specific gravity was found to be a good indication of hydration status and a practical method of improving workforce awareness and understanding of this important risk factor. Approximately 10 000 dehydration tests have been conducted under the dehydration protocol in a workforce of 2000 persons exposed to thermal stress and has proved practical and reliable.

Adult↗

Recirculation after cerebral ischemia. Simultaneous measurement of cerebral bloodflow, brain edema, cerebrovascular permeability and cortical EEG in the rat.

The 4-vessel occlusion rat model of cerebral ischemia was modified to permit the simultaneous measurement of cerebral blood flow (hydrogen clearance), brain edema (specific gravity), cerebrovascular permeability (14C-AIB) and electrocardiogram. Surgery was performed in one stage in the anesthetised, paralysed and ventilated rat and severe hemispheric ischemia was produced in all animals. Electrode implantation did not alter cortical specific gravity or Ki for 14C-AIB. During 4-vessel occlusion mean cortical CBF was 5.8 +/- 1.4 ml-1 100 g-1 min. and this was associated with an isoelectric ECoG; 15 min of ischemia produced a significant reduction in mean cortical specific gravity (increase in brain edema). Following 15 min ischemia, 180 min of recirculation were permitted. Post-ischemic blood flow showed an immediate hyperemia (CBF = 202 +/- 12 ml-1 100 g-1 min.) followed by hypoperfusion (CBF = 58 +/- 8 ml-1 100 g-1 min). There was an early further decrease in cortical specific gravity. Further recirculation led to a significant increase in cortical specific gravity (resolution of brain edema). The transfer constant (Ki) for 14C-AIB was not altered at any stage in recirculation. This appears to be a model of pure cytotoxic edema until 180 min recirculation after 15 min cerebral ischemia. Recirculation permitted return of cortical electrical activity.

Animals↗

The effect of air bubbles on rabbit blood brain barrier.

Several investigators have claimed that the blood brain barrier (BBB) may be broken by circulating bubbles, resulting in brain tissue edema. The aim of this study was to examine the effect of air bubbles on the permeability of BBB. Three groups of 6 rabbits were infused an isoosmotic solution of NaCl w/macrodex and 1% Tween. The solution was saturated with air bubbles and infused at rates of 50-100 ml hr(-1), a total of 1.6, 3.3, or 6.6 ml in each group, respectively. Two groups, each consisting of 6 rabbits, served as controls; one was infused by a degassed isoosmotic NaCl solution and one was sham-operated. All animals were left for 30 min before they were sacrificed. Specific gravity of brain tissue samples was determined using a brombenzene/kerosene gradient column, where a decrease in specific gravity indicates local brain edema. Specific gravity was significantly lower for left (P = 0.037) and right (P = 0.012) hemisphere white matter and left (P = 0.0015) and right (P = 0.002) hemisphere gray matter for the bubble-infused animals compared to the sham-operated ones. Infusion of degassed NaCl solution alone affected white left (P= 0.011) and right (P= 0.013), but not gray matter of both hemispheres. We speculate that insufficient degassing of the fluid may cause the effect of NaCl solution on the BBB of the white matter, indicating that the vessels of the white matter are more sensitive to gas bubbles than gray matter. Increasing the number of infused bubbles had no further impact on the development of cerebral edema, indicating that a threshold value was reached already at the lowest concentration of bubbles.

Air↗

Influence of hypoxic and hypercapnic acidosis on brain water content after forebrain ischemia in the rat.

OBJECTIVE: To determine whether hypoxia or hypercapnia superimposed on ischemia affects brain water content after ischemia. DESIGN: Prospective, randomized, controlled trial. SUBJECTS: Thirty-one male Wistar rats. INTERVENTIONS: The rats were assigned randomly into one of six groups: a) control; b) ischemia; c) ischemia combined with hypoxia; d) ischemia combined with hypercapnia; e) hypoxia; f) hypercapnia. Forebrain ischemia was induced for 5 mins by clamping both carotid arteries and inducing exsanguination. Either hypoxia or hypercapnia was induced until the arterial pH decreased to 7.0. The rats were decapitated after the protocol. MEASUREMENTS AND MAIN RESULTS: After the decapitation, the specific gravities of the neocortex, caudatoputamen, hippocampus, cerebellum, and midbrain were measured using a variable-density bromobenzene-kerosene column technique as an index of brain swelling. The specific gravities of the hippocampus and the neocortex were significantly lower in the ischemic group than in the control group. Specific gravities of the caudatoputamen and neocortex in the ischemia plus hypercapnia group, and specific gravities of the caudatoputamen, neocortex, and hippocampus in the ischemia plus hypercapnia group, were significantly lower than in the ischemia group. CONCLUSIONS: Cerebral water content increases more when ischemia is accompanied by hypoxia or hypercapnia than after ischemia alone. Hypoxic and/or hypercapnic acidosis during the periresuscitation period may be one of the causes of brain swelling after the resuscitation of patients after an anoxic-ischemic insult.

Acidosis, Respiratory↗

A rodent model of infusion brain edema: methodology and pathophysiological effects of saline and protein infusions.

To evaluate the potency of putative secondary mediators of brain edema and their possible contribution to edema related brain dysfunction an infusion model of brain edema was developed in rats. 100 ul of fluid (saline, 20% nonautologous protein) was infused over one hour into the left forebrain white matter through a stereotaxically placed (+1.2 mm ant to bregma, 3 mm lateral and 2.9 mm depth) 25 G needle. Brain tissue hydraulic resistance (Rt), regional cerebral blood flow (rCBF), cortical somatosensory evoked potentials (SEPs) and intracranial pressure (ICP) (intraventricular needle) were monitored during the infusion and rCBF CO2 reactivity (hydrogen clearance), local brain water content (microgravimetry), BBB integrity (Evans Blue 2%) and brain histology (H & E. Solochrome-cyanin) were evaluated after the infusion. Saline infusates caused no physiological dysfunction despite ipsilateral expansion and vacuolation of the subcortical white matter, separation of axonal bundles and a significant decrease (p = 3.8 x 10(-5] in local subcortical tissue specific gravity. Cortical histology and specific gravity adjacent to the infusion locus were normal. Rt significantly decreased (p = 6.5 x 10(-4] during the infusion but there were only minor increases in ICP. Findings with 20% protein infusates were similar despite a focal 65% decrement in the rCBF CO2 reactivity adjacent to the infusion site. This study has shown that a simple and inexpensive model of infusion brain edema can be created in the rat and that it provides a useful model for assessing the physiological effects of mediator compounds in the infusate. Potential applications and methodological improvements for this model are discussed.

Animals↗

[What the gravity environment enables us to attain].

How human beings achieve visual stability during body movements in space was investigated. When humans are exposed to an environment with different gravity environment, the pre-programmed behavior on the ground must be changed and should be re-programmed. This is habituation or familiarization. Present experiment focuses on visual, vestibular and somatosensory perception coordination and how it changes in microgravity as compared to a 1-G environment. We examined these issues under the microgravity during the mission Spacelab Japan (SL-J) mission with the cooperation of the Japanese Payload Specialist (RS), Dr. Mohri. In this paper we describe how the first human life science experiments were conducted and showed the unusual muscle cooperation between gravity specific and non-gravity specific muscle over the mission days.

Adaptation, Physiological↗

Collagen and non-collagenous proteins in different mineralization stages of human femur.

Mineralized tissues exhibit varying degrees of mineralization in different areas within the same bone. Using the technique of density gradient fractionation, bone powder from the diaphysis of human femur has been separated in different fractions corresponding to the degree of mineralization. Isolated bone fractions were analysed for their content in collagen and non-collagenous proteins. The results showed marked differences between compact and spongy bones, this latter containing higher proportions of little mineralized bone particles than the former (p less than 0.01). The ethylenediaminetetra-acetic acid (EDTA) extractability and the bone matrix size decreased relative to the decrease in specific gravity of bone particles. Among the matrix components of different fractions, sialoprotein consistently increased with the increase in specific gravity while proteoglycan decreased in reverse manner to the increase in collagen. However, in the most mineralized fraction (specific gravity: 2.33 g/cm3), the proteoglycan amount increased while collagen decreased. In conclusion, this study of bone maturation in human femur confirms the suitability of the technique of density gradient fractionation in the studies of bone matrix-mineral interactions. Apart from the fraction with the highest specific gravity, the analytical results obtained in fractions are similar to those observed in age-related bone changes, suggesting that the increase in mineralization degree of bone particles may be related to their age.

Aging↗

Cerebral water content in silicone oil-induced hydrocephalic rabbits.

Specific gravity determinations were made on fresh and desiccated samples of hydrocephalic rabbit brains. There was a density gradient in the normal cortical gray matter. Hydrocephalus was accompanied by significantly increased specific gravity of cortical gray matter at 3 days and 1 and 4 weeks. In the latter two periods, there were also significant increases in the specific gravity of white matter in the corpus callosum. These changes represent water loss throughout the whole cerebrum except at the ventricular surface. The specific gravity of solid tissue brain components changed insignificantly. We conclude that short-term brain volume changes in experimental hydrocephalus were due mainly to loss of tissue water.

Animals↗

Evaluation of the retention of electronic identification boluses in the forestomachs of cattle.

A total of 1,203 beef calves were used to evaluate 2 series of electronic identification boluses. Calves were intensively fattened and slaughtered at approximately 1 yr of age. Series 1 (n = 576 calves) consisted of 10 types of boluses with the same external dimensions (o.d. x length: 21 x 68 mm) but varying in weight (11 to 75 g) and specific gravity (0.63 to 3.36). Six boluses were made of ceramic (5 prototypes and 1 commercial bolus) and 4 were tubes made of plastic filled with concrete. Series 2 (n = 627 calves) consisted of 3 prototypes and 5 commercial boluses of different ceramic materials varying in external dimensions (o.d.: 15 to 21 mm; length: 39 to 78 mm), weight (20 to 73 g), and specific gravity (3.00 to 3.87). Boluses were administered to milk-fed calves (2 to 5 wk of age) by using adapted balling guns. To determine the anatomical limit for a bolus passing through the gastrointestinal tract, the size of the reticuloomasal orifice was measured in 90 male and 62 female fattened calves at slaughter. Three calves in series 1 (0.3%) could not swallow the 21-mm (o.d.) bolus at the first attempt and received the bolus 1 wk later. No problems for early administration were found with thinner boluses (o.d. < 20 mm) in series 2, and no signs of disease or growth alteration were detected in any bolused calves. Retention rate until slaughter varied according to bolus features and ranged from 0 to 100% (series 1), and from 69.7 to 100% (series 2). Inadequately dimensioned boluses were regurgitated or passed through the gastrointestinal tract and were excreted with the feces. The diameter of the reticuloomasal orifice differed between male and female yearling calves (32.5 and 29.9 mm, respectively; P < 0.01) and was greater than the o.d. of the retained boluses. Retention rate was predicted from bolus weight and volume by a logistic regression (R2 = 0.99, P < 0.001), in which the minimum bolus weight estimated to reach a 99.5% retention rate was 61 g when volume and specific gravity were 22.4 mL and 2.72, respectively. To achieve an effective retention rate of electronic identification boluses in the forestomachs of fattening calves, bolus volume and specific gravity, in addition to weight, should be optimized. No boluses of specific gravity lower than 3.0 and thicker than 20 mm o.d. are recommended for identification of cattle from early rearing (< 20 d of age) to slaughtering.

Animal Identification Systems↗

Effect of indomethacin and a free radical scavenger on cerebral blood flow and edema after cerebral artery occlusion in cats.

Using the middle cerebral artery occlusion model in cats, we evaluated the possible role of the cyclooxygenase pathway in alterations of local cerebral blood flow and the development of cortical edema following prolonged ischemia or recirculation. We divided 57 cats into three groups, and each cat received saline (control), indomethacin, or the free radical scavenger ONO-3144. Each group was subdivided into prolonged ischemia (4 hours of occlusion: PI) and recirculation (2 hours of occlusion followed by 2 hours of recirculation: RC) subgroups. We compared local cerebral blood flow and cortical specific gravity between the PI and RC subgroups of the control and drug-treated groups. In the PI subgroup, indomethacin did not influence the time course of local cerebral blood flow but significantly worsened the decrease in cortical specific gravity. On the other hand, indomethacin significantly improved postischemic hypoperfusion and ameliorated the decrease in cortical specific gravity in the RC subgroup. The effects of ONO-3144 were similar to those of indomethacin, except that ONO-3144 did not affect cortical specific gravity in the PI subgroup. Indomethacin inhibits cyclooxygenase activity, whereas ONO-3144 scavenges the oxygen-centered radical released in the conversion of prostaglandin G2 to prostaglandin H2. Thus, prostaglandins do not seem to play a major role in the occurrence of brain edema due to prolonged regional ischemia. By contrast, oxygen-centered radicals released from the cyclooxygenase pathway appear to be at least partially responsible for the occurrence of recirculation-induced edema and postischemic hypoperfusion.

Animals↗

Cerebrovascular permeability and brain edema after cortical photochemical infarcts in the rat.

The importance of protein extravasation for the development of vasogenic brain edema is still controversial. We, therefore, assessed the cerebrovascular permeability to serum proteins in relation to the development and resolution of brain edema in a photochemical cortical lesion in the rat. Cortical infarction was induced by in situ thrombosis using an argon laser beam aimed at the exposed parietal bone in animals given rose bengal i.v. The histology and the cerebrovascular permeability to serum proteins were scrutinized from 2 h to 3 weeks after the insult. The presence of serum proteins was demonstrated by an immunoperoxidase technique. The cerebral water content was estimated by specific gravity measurements of the cortical tissue in a kerosene-monobromobenzene gradient column from 2 h to 7 days after infarction. The blood-brain barrier was permeable to proteins at 2 h following the insult and proteins spread into the medial and lateral tissue reaching a maximum at 24 h. The specific gravity did not deviate from control values at 2 h. After 8 h the specific gravity of the lesion decreased with smaller decreases in the immediately adjacent tissue. At 24 h the changes in specific gravities reached a maximum in all regions except the immediately lateral area. The edema was generally worse in tissue medial to rather than lateral to the infarct. The degradation of serum proteins and the resolution of the brain edema followed the same time course with partial resolution of 72 h. By 1 week serum proteins and edema were confined to the central necrotic core. The results suggest a relationship between cerebrovascular permeability and cerebral edema in photochemical cortical infarction.

Animals↗

The effect of cropectomy on selected reproductive and physiological characteristics of laying hens.

Two-hundred and fifty Babcock B-300 pullets (18 weeks of age) were either cropectomized, sham-operated or used as unoperated controls in two experiments. Although feed consumption was slightly less in cropectomized birds than in control birds, the pattern of feed consumption in relation to time of day remained unchanged. Egg production and body weight of cropectomized birds were significantly lower than that of control or sham birds. Egg weight was not affected by cropectomy or the sham operation. Cropectomy had no influence on the pattern of serum calcium or on egg specific gravity in relation to time of day; however, serum calcium and egg specific gravity was consistently lower at each time interval measured throughout the day or night when compared to that of controls. The adverse effect of cropectomy on specific gravity of eggs and serum calcium indicated that the crop is an important organ in the laying hen. The crop appeared to serve as a storage depot for feed providing the bird with nutrients necessary for optimal shell quality during periods on non-consumption.

Animal Feed↗

A simple method to discriminate diapause from non-diapause pupae in large and small white butterflies, Pieris brassicae and P. rapae crucivora.

Differences in colour and shape have been used to discriminate diapause pupae from non-diapause pupae in butterflies. In the present study, we describe a simple discrimination method based on differences in the specific gravity of diapause and non-diapause pupae of large and small white butterflies, Pieris brassicae and Pieris rapae crucivora. When put into water, diapause pupae sink to the bottom (specific gravity is above 1.0), whereas non-diapause pupae float on the surface (specific gravity is below 1.0). Nuclear magnetic resonance microimaging revealed that this difference in specific gravity is due to a difference in the volume of an internal cavity located between the thorax and the abdomen in the pupae. The cavity appears quite early in development. We also examined the cavity in pupae of the unrelated swallowtail butterfly, Papilio xuthus, and found a similar cavity. However, in this species, the cavity is very small and, as a consequence, non-diapause as well as diapause pupae sink in water.

Animals↗

How gravity acts on Paramecium: new insights from free-fall experiments.

The swimming behaviour of Paramecium is affected by media of various specific gravities. At 1g, the negative gravitaxis of Paramecium virtually disappears in solutions the specific gravity of which is about the same as that of the organism (1.04). In solutions with a higher specific gravity (1.08), Paramecium becomes positively gravitactic. We recorded the swimming tracks of Paramecium in these media on videotape before, during and after free-falls. The records show that the density-dependent differences in the swimming behaviour disappeared immediately following the onset of the free-fall. The recorded tracks and distributions of cells in the experimental chambers were compared with computer-simulated traces and distributions based on gravitactic and gravikinetic models proposed for Paramecium. Our preliminary analysis favors a novel gravitactic mechanism involving modification of the ciliary movement The drop shaft at the Japan Microgravity Center, Hokkaido (JAMIC) was used for the free-fall experiments.

Animals↗