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PubMedDate not supplied

New low- and high-tech calendar methods of family planning.

Calendar-based methods are not usually considered effective or useful methods of family planning among health professionals. However, new "high-" and "low"-tech calendar methods have been developed, which are easy to teach, to use, and may be useful in helping couples avoid pregnancy. The low-tech models are based on a fixed-day calendar system. The high-tech models are based on monitoring urinary metabolites of female reproductive hormones. Both systems have high levels of satisfaction. This article describes these new models of family planning and the research on their effectiveness. The author proposes a new algorithm for determining the fertile phase of the menstrual cycle for either achieving or avoiding pregnancy.

Algorithms↗
PubMedDate not supplied

New approaches to fertility awareness-based methods: incorporating the Standard Days and TwoDay Methods into practice.

Helping clients select and use appropriate family planning methods is a basic component of midwifery care. Many women prefer nonhormonal, nondevice methods, and may be interested in methods that involve understanding their natural fertility. Two new fertility awareness-based methods, the Standard Days Method and the TwoDay Method, meet the need for effective, easy-to-provide, easy-to-use approaches. The Standard Days Method is appropriate for women with most menstrual cycles between 26 and 32 days long. Women using this method are taught to avoid unprotected intercourse on potentially fertile days 8 through 19 of their cycles to prevent pregnancy. They use CycleBeads, a color-coded string of beads representing the menstrual cycle, to monitor their cycle days and cycle lengths. The Standard Days Method is more than 95% effective with correct use. The TwoDay Method is based on the presence or absence of cervical secretions to identify fertile days. To use this method, women are taught to note everyday whether they have secretions. If they had secretions on the current day or the previous day, they consider themselves fertile. The TwoDay Method is 96% effective with correct use. Both methods fit well into midwifery practice.

Adult↗
PubMed2005

Molecular cloning and characterization of a cotton glucuronosyltranferase gene.

A glucuronosyltranferase gene has been isolated from cotton (Gossypium hirsutum) fiber cells using rapid amplification of the cDNA ends. The full-length cDNA, designated GhGlcAT1, is 1400 bp in length (AY346330) and contains an open reading frame of 1107 bp encoding a protein of 368 amino acids. Alignment of the GhGlcAT1 predicted amino acid sequence was shown to have high sequence similarity with animal glucuronosyltranferases. A phylogenic tree generated by the PHYLIP program package showed that GhGlcAT1 is clustered into the plant glucuronosyltranferase proteins and is distinct from those of other species. Homology modeling of the GhGlcAT1 structure using Homo sapiens native glucuronosyltranferase (1 kws and 1 fgg) structure as a template strongly suggests that the main-chain conformation and the folding patterns were similar to structural features characteristic of animal glucuronosyltranferases. Northern blot analysis showed that the transcripts of GhGlcAT1 were abundant in fiber cells, moderate in stem, but not detected in ovule, flower, seed, root and leaf. Transcripts were most abundant at 15dpa fiber. The transcription occurred at both the primary wall elongation stage and former stage of secondary cell thickening, suggesting that GhGLcAT1 may be involved in non-cellulose polysacchrides biosynthesis of the cotton cell wall.

Amino Acid Sequence↗
PubMed2004

Influence of repeated rectal ultrasound examinations on hormone profiles and behaviour around oestrus and ovulation in dairy cattle.

Frequent rectal ultrasound is often used to assess time of ovulation. This study investigated whether frequent rectal ultrasound examination, affects behavioural oestrus and peri-ovulatory hormone profiles (LH, oestradiol and progesterone). Additionally, the relation between peri-ovulatory hormone profiles, oestrous behaviour and time of ovulation was studied. Oestrus was synchronised in two consecutive cycles of Holstein Friesian cattle (parity from 1 to 6; n = 24 cycles). In 12 of these cycles, time of ovulation was assessed by three-hourly rectal ultrasound (assessment of ovulation time with ultrasound group: UG) the other half served as controls (n = 12; no assessment of ovulation time group: CG). There were no significant differences between the onset of oestrus (33.8 +/- 1.6 h), duration of oestrus (13.4 +/- 0.9 h) or intensity of oestrous behaviour (1047 +/- 180 points) between UG and CG treated animals. Furthermore, LH, oestradiol and progesterone profiles were similar between UG and CG. For UG, ovulation took place 30.2 +/- 1.9 h after onset of oestrus. This interval had the largest variation (21 h) of all parameters studied, ranging between 19 and 40 h after onset of oestrus. The smallest variation (6 h) was found in the timing of ovulation in relation to the LH-peak; ovulation took place 25.3 +/- 0.6 h (range: 21.5-27.5 h) after the peak in LH. This study demonstrated that repeated rectal ultrasound does not alter behavioural oestrus or peri-ovulatory hormone profiles and is therefore a useful tool for assessing time of ovulation. Further research, using ultrasound, can now be carried out to find predictors for time of ovulation.

Animals↗
PubMed2006

Luteal and follicular count in bitches: assessment by means of magnetic resonance imaging.

This study was done to determine whether preovulatory follicles or corpora lutea (physiological structures, PS) can be counted in the ovaries of bitches by means of magnetic resonance imaging (MRI). In Experiment 1, the ovaries from 15 German shepherd bitches (five in the follicular phase, one in the periovulatory period, five during the first 38 days of diestrus and four between Day 48 of diestrus and full-term gestation) were embedded in gelatin to form three phantoms with 10 ovaries each. Each phantom was exposed to MRI, using a 1mm slice thickness, a 1mm slice interval, a voxel size of 1mm cubic and a variety of pulse sequences, whereafter the ovaries were dissected and the numbers of follicles, corpora lutea and cysts counted. T2-weighted images were superior to T1-weighted images. Each of three operators counted the numbers of PS and cysts on T2-weighted images obtained in the coronal, transverse and sagital planes of each ovary, which, for the 30 ovaries, provided 270 operator by ovary by plane estimations and 90 operator by ovary estimations for each type of structure. Images of cysts were hyperintense, those of early corpora lutea and follicles similar and moderate and those of late corpora lutea hypo-intense and not clearly discernable from ovarian stroma. Estimations of PS were too low in 68%, correct in 12% and too high in 20% of estimations (n=270). Estimations of PS were correct in three operator by ovary combinations, out by 1 in 22 and out by more than 1 in 65. No operator estimated PS correctly in any bitch. In Experiment 2 MRI was done on three deeply sedated bitches in the periovulatory phase in an attempt to obtain images of the ovaries in order to count the follicles. The acquisition time of 5-7 min rendered images of poor quality from live bitches and none of their ovaries could be seen. MRI is not suitable for counting follicles or corpora lutea in the ovaries of bitches.

Animals↗
PubMed1991

Natural family planning: looking ahead.

Natural family planning is used by a relatively small number of people worldwide. The Institute's goal is to increase the knowledge, availability, effectiveness, and acceptability of natural family planning. To do so, the Institute is conducting biomedical research in several areas including: (1) the development of a home test kit for ovulation prediction, (2) sperm-mucus interaction, (3) the mechanisms and fertility impact of lactational amenorrhea, and (4) outcome of pregnancies in natural family planning users.

Amenorrhea↗
PubMed1991

Natural family planning and sex selection: fact or fiction?

Determining sex of offspring by timing of intercourse has been a subject of intense interest for the lay public and professional community. This metaaanalysis of couples practicing natural family planning provides an opportunity to evaluate sex ratios in relation to the timing of conception with several parameters, including basal body temperature shift and peak mucus as markers of ovulation. Data from six studies show a statistically significant lower proportion of male births among conceptions that occur during the most fertile time of the cycle.

Body Temperature↗
PubMed1991

Use of the Home Ovarian Monitor in pregnancy avoidance.

The application of the Home Ovarian Hormone Monitor to the avoidance of pregnancy by periodic abstinence has been explored. No woman had difficulty with the daily urine testing, and their results consistently identified the distinctive hormone pattern of the ovulatory cycle and the day of ovulation and correlated closely with the mucus symptoms. The tests gave 4 days or more warning of ovulation in 99% of cycles and allowed intercourse to be resumed 1 to 3 days after ovulation in 88%, giving a mean period of abstinence of 7 days. No pregnancy occurred from intercourse during the late safe days defined by the Monitor, but some early-day pregnancies occurred through long sperm survivals of 6 to 8 days, mostly during the return of fertility after breastfeeding. Rules for the avoidance of pregnancy, with the minimum of testing on the basis of these results, are given.

Adult↗
PubMed1987

Natural family planning.

It is now well accepted that a woman can conceive from an act of intercourse for a maximum of only about 7 days of her menstrual cycle. The reliability of natural family planning depends on identifying this window of fertility without ambiguity. Several symptomatic markers, cervical mucus and basal body temperature, have been used extensively and with considerable success in most women but failures occur. Ovarian and pituitary hormone production show characteristic patterns during the cycle. Urinary estrogen and pregnanediol measurements yield reliable information concerning the beginning, peak, and end of the fertile period, provided that the assays are accurate and performed on timed specimens of urine. We have developed such enzyme immunoassays for urinary estrogen and pregnanediol glucuronides that can be performed at home. In the early versions of the assays, enzyme reaction rates were measured by eye, but more recently, a simple photoelectronic rate meter has been used. The final problem to be solved is not technologic but whether women are sufficiently motivated to expend the same time and effort each day for 10 days a month, with less cost, on fertility awareness as they spend on making a cup of tea.

Adult↗
PubMed2001

Further analysis of the theoretical effectiveness of the TwoDay method of family planning.

This article validates the theoretical effectiveness of a simple approach to identify the fertile window of the menstrual cycle. The TwoDay method identifies all days in the cycle in which the woman notices cervical secretions, and the days immediately following these days, as the period in which the woman should consider herself fertile. Women who use this method are counseled to avoid unprotected intercourse on these days. The theoretical effectiveness of the TwoDay method was tested previously by applying the method rules to the menstrual cycles of women from a large data set from the World Health Organization (WHO). For the current study, we administered the same analysis to a data set from an Italian Ovulation Method center. These data are better suited for the analysis than were the WHO data because they identify all days with secretions. Results suggest that the method can be highly effective in helping women to identify correctly the days on which they should avoid unprotected intercourse if they do not wish to become pregnant, although some users may identify a few days as fertile that actually are not.

Adult↗
PubMed2002

Accuracy of the peak day of cervical mucus as a biological marker of fertility.

The (PD) peak day of cervical mucus is an important biologic marker for the self-determination of the optimal time of fertility in a woman's menstrual cycle. The purpose of this article is to provide evidence (literature and empiric) for the accuracy of the PD of cervical mucus as a biologic marker of peak fertility and the estimated day of ovulation. An analysis of data from four published studies that compared the self-determination of the PD of cervical mucus with the urinary luteinizing hormone (LH) surge was conducted. The four studies yielded 108 menstrual cycle charts from 53 women participants. The 108 cycles ranged in length from 22 to 75 days (mean 29.4 SD 6.0). Ninety-three of the 108 cycles had both an identified PD and LH surge. Data charts showed that 97.8% of the PD fell within +/-4 days of the estimated day of ovulation. Use of a standardized mucus cycle scoring system indicated that the peak in cervical mucus ratings was highest on the day of the LH surge. Self-determination of the PD of cervical mucus is a very accurate means of determining peak fertility and a fairly accurate means of determining the day of ovulation and the beginning of the end of the fertile time.

Adult↗
PubMed1981

Correlation of plasma gonadotrophins and ovarian steroids pattern with symptomatic changes in cervical mucus during the menstrual cycle in normal cycling women.

27 healthy young Italian women were studied to evaluate their ability to identify symptomatically the potentially fertile phase of the menstrual cycle by self observation of their cervical mucus pattern as described in the Ovulation Method Billings. The women's observations were correlated with daily plasma levels of FSH, LH, estradiol-17 beta and progesterone. Ovulation was considered to occur on the day following the LH peak. The hormonal assays revealed that 2 of the 34 cycles studied were anovulatory. 24 of the 27 subjects in the study group demonstrated their ability to recognize the onset of the mucus discharge and the peak symptom from the first cycle after teaching, another two from the second cycle. The only other subject contributed an anovulatory cycle in which the hormonal assay confirmed the accuracy of her mucus observations. In the study, the mean interval between the time of ovulation as assessed and the peak symptom recorded by the subjects was 0.0 days, with a range from -2 to +1 days. The mean time interval from the first recorded symptom to the estimated day of ovulation was 6.0 days, with a range from 3 to 10 days. The study shows that young Italian women can be taught to recognize their cervical mucus pattern as described in the Ovulation Method Billings. The accuracy of their observations is demonstrated by the hormonal assays. The study also confirms the conclusion reached in earlier similar studies that there is a direct correlation between the cervical mucus symptom and the potentially fertile phase of the cycle. Research is currently being conducted on a larger number of couples employing the Ovulation Method Billings to actually regulate their fertility.

Adolescent↗