Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “INSEMINATION”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 523 records · Page 29Linked to original sources
PubMed2002

Hysteroscopic insemination of low numbers of flow sorted fresh and frozen/thawed stallion spermatozoa.

The objective of this experiment was to determine the effects of flow cytometric sorting and freezing on stallion sperm fertility. A 2 x 2 factorial design was used to delineate effects of flow sorting and freezing spermatozoa. Oestrus was synchronised (July-August) in 41 mares by administering 10 ml altrenogest (2.2 mg/ml) per os for 10 consecutive days, followed by 250 microg cloprostenol i.m. on Day 11. Ovulation was induced by administering 3,000 iu hCG i.v. either 6 h (fresh spermatozoa) or 30 h (frozen/thawed spermatozoa) prior to insemination. Mares were assigned randomly to one of 4 sperm treatment groups. Semen was collected from 2 stallions with an artificial vagina and processed for each treatment. Treatment 1 (n = 10 mare cycles) consisted of fresh, nonsorted spermatozoa and Treatment 2 (n = 16 mare cycles) of fresh, flow sorted spermatozoa. Spermatozoa to be sorted were stained with Hoechst 33342 and sorted into X- and Y-chromosome-bearing populations based on DNA content using an SX MoFlo sperm sorter. Treatment 3 (n = 16 mare cycles) consisted of frozen/thawed nonsorted spermatozoa (frozen at 33.5 x 106 sperm/ml in 0.25 ml straws) and Treatment 4 (n = 15 mare cycles) of flow sorted frozen/thawed spermatozoa (frozen at 64.4 x 10(6) sperm/ml). Concentrations of sperm in both cryopreserved treatments were adjusted, based on predetermined average post-thaw motilities, so that each insemination contained approximately 5 x 10(6) motile spermatozoa. Hysteroscopic insemination of 5 x 10(6) motile spermatozoa in a volume of 230 microd was used for all treatments. Pregnancy was determined ultrasonographically 16 days postovulation. No differences were found (P>0.1) in the pregnancy rates for mares inseminated with fresh nonsorted (4/10 = 40.0%), fresh flow sorted (6/16 = 37.5%), frozen/thawed nonsorted (6/16 = 37.5%) and flow sorted frozen/thawed spermatozoa (2/15 = 133%). Pregnancy rates tended (P = 0.12) to be lower following insemination of frozen/thawed flow sorted spermatozoa. Further studies are needed with a larger number of mares to determine if fertility of flow sorted frozen/thawed spermatozoa can be improved.

Animals↗
PubMed2004

Effect of simultaneous thawing of multiple 0.5-mL straws of semen and sequence of insemination on conception rate in dairy cattle.

The objectives of this study were to determine 1) the effect of simultaneous thawing of multiple 0.5-mL straws of semen and sequence of insemination (first, second, third, or fourth) on conception rates in dairy cattle, 2) whether the conception rates achieved following AI by professional AI (PAI) technicians and herdsman-inseminators (HI) differed, and 3) the effect of elapsed time from initiation of thawing straws of semen to seminal deposition on conception rates in dairy cattle. Four dairies with PAI and four with HI participated in the study. Initial data recorded included beginning thaw time, cow identification number, and time of seminal deposition. Herd records were retrieved following pregnancy diagnosis. Conception rates of dairy cows (n = 1025) were not affected by sequence of insemination (first, second, third, or fourth). Conception rates for herds using PAI were 40, 47, 41, and 50%. Conception rates for herds using HI were 24, 20, 33, and 30%. Average conception rates of dairy cows differed between PAI and HI (45 vs. 27%, respectively). The difference in mean conception rate achieved by PAI and HI was not attributable to milk production, parity, service number or stage of lactation. The elapsed time from initial thaw to completion of fourth AI was shorter for PAI than for HI, 7.6 vs. 10.9 min, respectively. Although the average conception rate differed between PAI and HI, elapsed time from initial thaw to completion of fourth AI and sequence of insemination (first, second, third, or fourth) had no effect on conception rate within inseminator group.

Animals↗
PubMed1982

Influence of season on timed inseminations of dairy heifers synchronized by prostaglandin F2 alpha.

Sixty-six cycling Holstein heifers were given two injections of prostaglandin F2 alpha (25 mg intramuscular) at an 11-day interval. Average time of first observation of estrus differed little between July (55.7 +/- 5.3 h) and December, 1980 (50.6 +/- 5.1 h). Heifers were inseminated artificially at either 65 or 80 h following the second injection of prostaglandin. Synchronized heifers inseminated at 65 h had a higher conception rate in July (9/18; 50%) than in December (3/15; 20%). However, heifers inseminated at 80 h had a similar conception rate in July (9/18; 50%) and in December (8/15; 53%). Progesterone in blood serum was measured prior to the second prostaglandin F2 alpha injection before insemination and on day 21 postbreeding. Averages of progesterone for the 65-h group were 3.91 +/- .48, .39 +/- .07, and 3.80 +/- .76 ng/ml in July and 4.25 +/- .44, .33 +/- .08, and 3.91 +/- .63 ng/ml in December for the three collections. Averages for the 80-h group were 3.62 +/- .28, 1.06 +/- .56, and 3.60 +/- .65 ng/ml in July and 2.17 +/- .35, .22 +/- .07, and 2.77 +/- .43 ng/ml in December. An influence of season on response to prostaglandin and timed inseminations in dairy heifers may affect estrus behavior and conception rate.

Animals↗
PubMed1984

Effects of serum treated semen, bulls, and herdsmen-inseminators on conception to first service in large commercial dairy herds.

Semen from each of 10 Holstein bulls was extended to 15 X 10(6) total spermatozoa per dose in egg yolk-Tris and in egg yolk-Tris containing 20% (vol/vol) heat-treated heifer serum. Semen was used during 12 mo by 11 herdsmen-inseminators in four commercial dairy herds to inseminate 2820 Holstein cows on the first postpartum service. Percentage conception of cows free of clinical disorders at breeding was determined by rectal palpation at 30 to 60 days after insemination. Addition of serum to the extender did not influence percentage conception. A significant interaction of bull x seminal treatment was found. Least squares means of percentage conception to first service ranged from 45.3 to 59.9% among the 10 bulls. When bulls were classified as either high (greater than 55%), average (50 to 55%), or low conception (less than 50%), approximately 200 first services were required to classify correctly 80 to 90% of the bulls. Least squares means of conception rates ranged from 40.1 to 62.7% among the 11 herdsmen-inseminators. 1) Addition of heat-treated homologous heifer serum to the extender does not influence conception in a predictable fashion; 2) fertility inherent to the bull significantly influences conception; and 3) inseminator skill affects conception greatly.

Animals↗
PubMed1987

Effects of extender and insemination dose on postthaw quality and fertility of bovine sperm.

Quality and fertility of sperm extended in egg yolk-Tes-Tris were compared with those of sperm in egg yolk-citrate and homogenized milk extenders. Extended semen was frozen in .5-ml plastic straws at 11, 15, 17, or 22 X 10(6) sperm per insemination dose. Laboratory evaluations at 0, 1, 2, and 4 h after thawing semen utilized four tests of spermatozoal quality. Use of egg-yolk citrate extender resulted in a higher percentage of progressively motile sperm as determined visually at 0 h after thawing than use of egg yolk-Tes-Tris or homogenized milk extenders. Sperm extended in egg yolk-citrate had 18% lower activity of bound amidase at 0 h than sperm extended in egg yolk-Tes-Tris. The 75-d nonreturn rates were affected by insemination dose but not be extender or the interaction of extender and insemination dose. Fertility was lower after insemination of 11 X 10(6) sperm than for pooled data for the three higher insemination doses (64 vs. 68%). Based on all data, postthaw quality of sperm processed in the one-step egg yolk-Tes-Tris extender was similar to that for sperm extended in egg yolk-citrate or homogenized milk.

Animals↗
PubMed1989

Use of B-mode, linear array ultrasonography for evaluating the technique of bovine artificial insemination.

B-Mode, linear array ultrasonography was used to evaluate the site of semen deposition in live cows. The insemination sheath was modified to hold a brass bead that was deposited at the simulated site of semen deposition. The position of the bead was identified by ultrasonography and the bead was recovered by pulling a nylon line that was attached to the bead. An ultrasound technician identified placement of the bead in the site designated for semen deposition by a skilled inseminator in 24 of 25 cases. This procedure improves methods for inseminator training by providing a method of evaluating insemination technique using live animals rather than excised reproductive tracts. The ultrasound equipment used for inseminator evaluation is affordable, portable, and does not pose a human health risk.

Animals↗
PubMed1998

The relationship between the number of spermatozoa inseminated and the reproductive efficiency of individual dairy bulls.

The semen of 20 mature, evaluated bulls was split-sample diluted and contained 2.1 x 10(6) to 17.3 x 10(6) total spermatozoa per 0.25-ml French straw. The number of viable inseminated spermatozoa ranged from 1.1 x 10(6) to 11.8 x 10(6). Each bull had 2430 to 5330 first or second inseminations performed. The nonreturn rate at 56 d after AI was estimated for every dilution. The daily nonreturn rates to 180 d were used to estimate conception and calving rates at a given concentration. The relationship was determined between these estimations and the number of spermatozoa that were actually inseminated. The bulls differed significantly in their maximal nonreturn rate at high sperm numbers per AI and in the rate at which they approached this maximum. There was no correlation between the maximum nonreturn at high sperm numbers and the rate of approach, which implies that the ranking of the bulls for nonreturn rate 56 d after AI changes with the number of spermatozoa inseminated. Multiphasic analysis of reproductive efficiency revealed bull differences in estimated conception and calving rates. The estimated calving rate after conception was 82 to 90% and was independent of the number of spermatozoa that were inseminated. The sperm numbers needed to obtain 95% of the maximal conception rate ranged from 1 x 10(6) to 11 x 10(6).

Animals↗
PubMed1981

Effect of insemination intervals on oviducal sperm storage in turkeys.

Filling and emptying of sperm storage sites of the turkey oviduct were studied using tandem inseminations with semen from bronze and white males. Fertility was better among hens inseminated at weekly intervals than those inseminated at biweekly intervals. The rate of gland filling and emptying was unaffected by weekly and biweekly insemination intervals. However, gland filling and emptying were affected when the birds were inseminated early in a reproductive cycle, and stratification of spermatozoa occurred within the sperm-host glands. Stratification may have occurred to a lesser degree late in the reproductive cycle. It is suggested that sperm storage glands fill and empty at the same rates early and late in a breeding cycle, but fewer glands contain spermatozoa in the latter part of the season, resulting in a decreased fertility.

Animals↗
PubMed2001

Deep intrauterine insemination and embryo transfer in pigs.

A new method for non-surgical deep intrauterine catheterization of pigs, without sedation of the sow, is described. Insemination results obtained with this method using fresh spermatozoa demonstrate that, in comparison to conventional artificial insemination (AI) (3 x 10(9) spermatozoa in 80-100 ml), a 20-60-fold reduction in the number of spermatozoa inseminated and at least a 8-10-fold reduction in the dose volume can be used without affecting fertility if spermatozoa are deposited deep (middle or upper) into one of the uterine horns. Results from deep intrauterine insemination with frozen-thawed spermatozoa and flow-sorted spermatozoa are also presented and the effect of deep intrauterine insemination on sperm transport is discussed. In addition, a brief description of the advances made in non-surgical embryo transfer technology is reported.

Animals↗
PubMed1990

Experimental transmission of bovine viral diseases by insemination with contaminated semen or during embryo transfer.

Three experimental approaches were used to study transmission of blue tongue (BT), infectious bovine rhinotracheitis (IBR) and bovine virus diarrhoea (BVD) viruses. These were insemination with contaminated semen, experimental infection of embryo donor cows, or transfer of embryos experimentally exposed to virus in vitro to normal recipients. Parameters assessed included number and quality of embryos produced, virus detection (isolation and electron microscopy), serology and histopathology. All superovulated sesceptible cows inseminated with semen containing blue tongue virus (BTV) (n = 2) or infectious bovine rhinotracheitis virus (IBRV) (n = 2) became infected. One cow inseminated with semen containing BTV produced seven virus-free seven-day-old embryos; the second cow failed to produce any embryos. One of two cows inseminated with semen containing IBRV produced two underdeveloped, virus-free embryos while no embryos were produced by the second cow. One of two cows inseminated with semen containing bovine viral diarrhoea virus (BVDV) became infected. Two poorly developed, virus-free seven-day-old embryos were recovered from one of these cows. Superovulated susceptible cows inoculated either intramuscularly with BTV (n = 3) or intranasally with IBR virus (n = 2) became infected. Virus was isolated from some tissues of two BTV-infected cows, neither of which produced embryos. A third BTV-infected cow produced two virus-free embryos collected at necropsy five days after inoculation. One of two cows experimentally infected with IBR virus, produced three embryos but virus was not detected either by electron microscopy (1 embryo) or in cell culture by cytopathic alterations (1 embryo).(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1986

[Fertilization results in superovulated ewes and goats after transmural-intrauterine insemination controlled by laparoscopy].

A comparative experiment in oestrous synchronized and superovulated ewes and does is described on which fertilization was studied following natural mating or transmural intrauterine insemination by laparoscopy. Thirty-two ewes were inseminated into each uterine horn with 50-100 microliter of fresh undiluted semen, whereas 26 ewes were mated by fertile rams. Fertilization rates of eggs recovered 5-7 days later were 93.7% (163/174) and 60.5% (78/129) respectively. Eight does were inseminated by laparoscopy while 12 does were mated by fertile bucks. Numbers of eggs fertilized of those recovered 6-7 days later were 42/47 (89.4%) in the intrauterine inseminated group and 51/60 (85%) in the naturally mated group. These results indicate that high levels of fertilization can be obtained in ewes following intrauterine insemination with fresh semen with the aid of a laparoscope and that it is possible to overcome the reduced fertilization rates after natural mating. In contrast to the ewe the intrauterine semen deposition in does did not improve the high fertilization rates obtained after natural mating.

Animals↗
PubMed1988

Current topics in artificial insemination of sheep.

There have been developments in several aspects of artificial insemination (AI) in recent years, some of which have been directly responsible for proliferation of AI in the sheep-breeding industries of several countries. The most notable advances have probably been associated with the development of intrauterine insemination by laparoscopy. There is potential for refinement of some of the related techniques, particularly in the area of control of ovulation and definition of appropriate times and optimum doses of spermatozoa for insemination. It is unlikely that laparoscopic AI will be developed sufficiently that it will become readily affordable, and therefore widely practised, by commercial producers. Unfortunately, there has been little progress in the past few years in improvement of the methods of cryopreservation of ram semen. There is considerable potential for AI to have a significant impact on the genetic improvement of sheep, though this has yet to be evaluated in practice. However, if the full potential of AI in sheep is to be realized, it will likely only happen when methods of freezing semen are improved sufficiently that cervical or even vaginal insemination can be widely used with frozen-thawed semen, or when practicable methods of deep cervical or intrauterine insemination through the cervix are developed.

Animals↗
PubMed1986

[Sperm collection, evaluation, preservation and artificial insemination in the dog].

The present paper explains the conditions for semen collection in the dog in principal and the technique of semen collection by manual stimulation of the penis in detail. The evaluation of the most important ejaculate parameters is pointed out concerning their dependence of the partially striking variation of the body size of dogs from different breeds. Furthermore some methods of semen preservation and the results of the laboratory examinations are presented. The notes upon artificial insemination contain the time, frequency, interval and technique of semen application. Better conception rates can be obtained by a double than by a single insemination. Using fresh semen an insemination interval of 48 hours has proved favorable whereas with preserved semen the insemination interval should not exceed 24 hours. While freshly ejaculated semen can be applied with good success intravaginally, the intrauterine insemination offers better conditions for conception using liquid or frozen stored semen.

Animals↗
PubMed1984

[Correlation between the number of inseminated spermatozoa and results of fertilization in dairy cattle].

Of forty-seven Friesian bulls 216 ejaculates were split-samples and diluted to concentrations varying from 3.75 to 40 million spermatozoa per insemination dose. The semen was frozen in 0.25 ml French straws. After freezing, the number of spermatozoa per straw was checked with an electronic particle counter. Optimum fertility rates were achieved with a concentration of 15 million spermatozoa per insemination dose. Higher numbers of spermatozoa per insemination did not result in a further increase in conception rates. The optimum dosage of spermatozoa was dependent on the fertility level of the bull. In bulls with high, average or low fertility, the maximum fertility levels attainable were achieved with 7.5, 10 or 15 million of spermatozoa per insemination dose respectively. An interaction between season and insemination dose could not be shown to be present.

Animals↗
PubMed1984

[Effect of the type of restraint of sheep during artificial insemination on their fertility].

Studies were carried out to establish the effect of the various methods of restraining the sheep at their artificial insemination on the rate of conception. It was found that as a result of the fixing technique practiced up to then with sheep stress conditions developed that varied in number and effect, which led to a lower conception rate. The use of a new technique and technology of fixing and inseminating the sheep, with which all unfavourable stress factors were fully avoided, raised the conception rate by 7 to 14 per cent. A new equipment was used that made unnecessary the driving, seising; and lifting the animals as they themselves passed onto the platform for fixing and insemination. With the employment of the new method the enhanced vaginal contractions, which stood in the way of proper insemination, were lowered from 26 to 8.2 per cent as against the old technology. The new equipment further made it possible to shorten the time of inseminating each animal twice to three times and thus shorten the time for the semen to stay out of the animal body. This, in turn, also produced a favourable effect on the impregnation of sheep.

Animals↗
PubMed1981

[Menstrual cycle changes during consecutive artificial insemination therapy and their treatment].

Emotional stress-induced problems generated by the application of artificial insemination therapy by donor (AID) are considerable. After 6 months of frustrane insemination treatment, 54% of the women exhibited irregular or anovulatory cycles. Especially the persistent late ovulation often leads to a failure of the mechanisms of conception. An early ovulation timing with high dosages of synthetic LH-releasing hormone or a stimulating LH-RH-analogue (HOE 766) lead to an increasing pregnancy rate after application in the third insemination cycle from 34,0% untreated to 51,1% treated. The early application of ovulation inducing or timing substances is underlined in a program of artificial insemination therapy to eliminate possible stress-induced failures at an early point of treatment. As the spontaneous pregnancy rate is high in the first treatment cycles, LH-RH or its analogue should be administered after two frustrane insemination cycles.

Anxiety↗
PubMed1980

The influence of heat symptoms and the time of insemination of cattle on the early and late returns.

In a material comprising 95,085 inseminations, the non-return rate and the frequency of late returns, measured as the frequency of returns, measured as the frequency of returns between 29 and 56 days, have been studied with respect to the time of insemination within the heat period and to the intensity of heat. Both these factors have effected the NR result in that insemination late in the oestrus period--2 days after heat has been observed--gives the poorest result, 60.5% 56 days NR in comparison with 68.5 one day after observation and 71.4 the same day. Weak heat symptoms give a bad result, 60.1 compared to 68.7 for fair and 72.7 for strong heat symptoms. The differences are significant. The number of late returns is also influenced by the time of insemination as well as by the intensity of heat. However, insemination in heats with weak symptoms leads to a higher frequency of late returns than what can be expected from the frequency of early returns. This might partly indicate an increased rate of early embryonic death.

Animals↗
PubMed1995

Use of surgical intrauterine insemination to manage infertility in a colony of research German shepherd dogs.

A colony of German shepherd dogs with inherited ventricular arrhythmias and sudden death had infertility that was resolved by intrauterine insemination. Mating of German shepherd males to related German shepherd females (40 cycles) by vaginal artificial insemination resulted in a low pregnancy rate of 35% and a small median litter size of 3.5. When these same German shepherd males were bred to female beagles by vaginal artificial insemination, the pregnancy rate of 100% (P = 0.02) and median litter size of 7.0 were significantly (P = 0.04) greater. Therefore, inadequate fertility existed when the German shepherds were mated. Because matings between these dogs were necessary, surgical intrauterine insemination of fresh semen was instituted in eight German shepherd females over nine cycles. In bypassing the cervix with this method, German shepherd fecundity increased significantly, with a pregnancy rate of 100% (P = 0.002) and median litter size of 8.0 (P = 0.001). Surgical intrauterine insemination may be an important method for management of canine infertility in the research environment.

Animals↗