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PubMed1995

Determining the net energy value of wet and dry corn gluten feed in beef growing and finishing diets.

One growing and two finishing trials evaluated the feeding value of corn gluten feed for cattle. In the growth trial, calves were fed (DM basis) one of two control diets (44% dry-rolled corn [DRC], 50% alfalfa hay, 5% molasses, 1% supplement or 33% DRC, 33% alfalfa hay, 33% corn silage, 1% supplement), diets containing 49 or 65% wet corn gluten feed (WCGF) with alfalfa hay and supplement, or a diet containing 61% WCGF with cornstalks and supplement. Calves fed diets containing 49 or 65% WCGF with alfalfa hay gained faster (P < .10) and more efficiently (P < .10) than calves fed the two control diets. In finishing trial 1, yearling steers were fed 92.5% concentrate diets containing (DM basis) 79% DRC, a combination of DRC and 35 or 70% WCGF, a combination of DRC and 70% dry corn gluten feed (DCGF) with or without added water, or a combination of high-moisture corn (HMC) and 70% WCGF. Steers fed 35 or 70% WCGF in combination with either DRC or HMC had gains and efficiencies similar (P > .10) to those of steers fed DRC. Inclusion of water in the 70% DCGF diet reduced DMI (P < .10) and daily gain (P < .10) but did not affect (P > .10) feed efficiency. In Trial 2, WCGF replaced either 20, 40, 60, 80, or 100% of the DRC and molasses (DM basis). Feed efficiency was not different (P > .10) among diets.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗
PubMed1988

Effects of corn gluten feed on forage intake, digestibility and ruminal parameters of cattle fed native grass hay.

Thirty-two beef cows (467 kg) were individually fed native grass hay and supplement for two 14-d periods in each of 2 yr. Supplement treatments and amounts fed (kilograms/day) were negative control (NC), 0, or equal amounts of protein from soybean meal (SBM), .7; a blend of soybean meal and corn gluten feed (SBM/CGF), 1.0; or corn gluten feed (CGF) 1.6. Cows received supplement at 0645 and had ad libitum access to native grass hay from 0700 to 1130 and from 1530 to 2000. Compared with NC, all protein supplements increased (P less than .05) ruminal NH3, propionate and butyrate concentrations at 4 and 25 h postfeeding. Ruminal fluid pH, total VFA and acetate concentrations at 4 and 35 h postfeeding were not affected by supplements. All supplements increased (P less than .01) hay intake as well as hay, acid detergent fiber (ADF) and total diet dry matter (DM) digestibility. Compared to supplemental SBM, feeding CGF reduced (P less than .01) hay intake. Calculated daily intakes of metabolizable energy (ME) were 12, 17, 18, and 17 Mcal for NC, SBM, SBM/CGF and CGF, respectively. Hay intake, DM and ADF digestibility and ME intakes tended to be higher for SBM/CGF than for the average of SBM and CGF fed alone. Intakes of digestible DM and ADF were not altered by protein supplements, suggesting that intake responses were due to increased diet digestibility. Corn gluten feed appears to be an effective source of supplemental protein and energy for cows consuming low-quality roughage.

Animal Feed↗
PubMed1978

Gluten-sensitive enteropathy: genetic analysis and organ culture study in 35 families.

The genetic marker histocompatibility antigen HLA-B8 is present in 80% of patients with gluten-sensitive enteropathy (GSE). We studied 35 families with at least one affected member to determine whether an HLA-region gene alone could determine susceptibility to GSE. The incidence of HLA-B8 in the patients was 69% vs 22% for normals (P less than 0.001). The incidence of GSE in HLA-genotype-identical siblings of patients was only 8%, and in HLA-B8-haplotype-identical siblings and parents of patients was only 14% and 5%, respectively. In addition, intestinal biopsies of HLA-identical or partially identical relatives of patients were studied in an in vitro organ culture system capable of detecting gluten sensitivity in subjects ingesting a normal diet. The results confirmed the low incidence of gluten sensitivity in these individuals. The organ culture system could not differentiate mucosa obtained from unaffected parents or siblings of patients with GSE (who presumably carry the HLA-associated genetic information) from mucosa obtained from normals. We conclude that the genetic material inherited with HLA-B8 alone is not sufficient to produce clinical or subclinical disease. Other genetic and environmental factors appear to be important for disease pathogenesis.

Adolescent↗
PubMed1985

Studies of intestinal lymphoid tissue. IX. Dose-dependent, gluten-induced lymphoid infiltration of coeliac jejunal epithelium.

Jejunal biopsy specimens from coeliac patients who had received small, oral doses (100-1500 mg) of a peptic-tryptic gluten digest were analysed by morphometric methods. An increase in the total number of surface epithelial lymphocytes, maximal at 12 h after challenge, was dose-dependent, the mean percentage rise at this time being 53% (p less than 0.005), 44% (p = 0.01), and 25% (p greater than 0.05) with 1500, 1000, and 500 mg of gluten digest, respectively. This effect was not accompanied by any increased mitotic activity or blast transformation among the infiltrating lymphocytes, nor was there any demonstrable alteration in mucosal structure-that is, reduction in surface or increase in crypt epithelial volumes. The results of this controlled morphometric analysis indicate that oral gluten challenge causes an increase in the lymphocyte population of surface epithelium in coeliac disease but that this effect does not necessarily result in mucosal damage.

Celiac Disease↗
PubMed1985

Influence of gluten-free diet on the gastric condition in dermatitis herpetiformis.

Achlorhydric atrophic gastritis occurs in approximately 25% of patients with dermatitis herpetiformis (DH). The effect of gluten withdrawal on the gastric condition was studied in 35 patients, with a control group of 20 patients continuing their habitual diet. Gastrointestinal examinations were performed initially and repeated after about 1 3/4 years. Adherence to the diet was confirmed by dietary interviews, improvement of malabsorption test results and intestinal villous structure, and decreased dapsone requirement. Neither the non-restricted diet nor the gluten-free diet had any effect on gastric morphology, the ability to secrete gastric acid, serum gastrin levels, or the frequency or titres of circulating parietal cell antibodies. The findings indicate that gluten is not responsible for the perpetuation of the gastric affection in DH, in contrast to the enteropathy.

Adolescent↗
PubMed1994

Immunologic and intestinal permeability tests as predictors of relapse during gluten challenge in childhood coeliac disease.

Fifteen children with an initial diagnosis of coeliac disease underwent gluten challenge either because they had never had a jejunal biopsy or because they had had one during the first 2 years of life. The challenge was preceded by a biopsy; clinical symptoms, the cellobiose/mannitol permeability test, and gliadin and endomysial antibody measurement were used to determine the timing of the confirmatory biopsy: it was performed if one test result was repeatedly abnormal or two results were concomitantly abnormal. Gliadin antibodies increased early (already 7 days after the reintroduction of gluten to the diet), but in many cases they returned to normal values thereafter. Increased intestinal permeability to sugars and even more positivity of endomysial antibody were good predictors of histologic relapse. The sequential use of laboratory tests during gluten challenge may significantly shorten its duration.

Autoantibodies↗
PubMedDate not supplied

The immunopathology of the small intestinal reaction in gluten-sensitivity.

Computerised image-analysis was used to define the spectrum of immunopathological changes in small intestinal mucosa in established celiac sprue disease; dermatitis herpetiformis; 1 degree relatives of celiac sprue patients, and treated celiac sprue patients challenged with varying doses of a peptic-tryptic digest of gluten. Typically, in flat ('Type 2') lesion there was a reduced number of large, mitotically active lymphocytes in surface epithelium, but an increased lymphocyte population in crypts. In approximately 50% untreated DH patients and in 20% 1 degree celiac sprue relatives, mucosal architecture was well-preserved although surface (villous) epithelium contained an expanded population of small, non-mitotic lymphocytes ('Type 1' lesion), with or without crypt hyperplasia. Similar changes were also induced by small dose gluten challenge. Larger dose challenges caused a progression from a Type 1 to a Type 2 lesion during a 5 day period of observation. In addition, observations on a few patients over 2-4 years showed a similar sequence of mucosal changes. A major feature of this sequence was the early appearance of crypt hypertrophy, before significant villous flattening had occurred. These changes parallel T lymphocyte-mediated graft- versus-host reactions in animals, suggesting that the specific immunopathologic features seen in gluten sensitivity are fundamentally cell-mediated in type, the degree of change probably dependent on host genetic factors. Finally, these data show that in becoming flat the mucosa must initially pass through the earlier Type 1 lesion in which crypt hypertrophy is a prominent response.

Adult↗
PubMed1994

Corn gluten meal or dried brewers grains as partial replacement for soybean meal in the diet of Holstein cows.

Holstein cows (n = 24, 9 primiparous) were assigned equally to diets based on corn silage and high moisture corn supplemented with soybean meal alone or with either corn gluten meal or dried brewers grains. In diets with two protein sources, the protein quantity from each source was similar. Dietary CP and ADF averaged 16.3 and 18.8%, and undegraded protein as a percentage of CP was 33.6, 41.1, and 41.8% for the respective diets. After parturition, cows were fed the soybean meal diet for a 3-wk covariant period, an experimental diet for 10 wk, the soybean meal diet for 3 wk, and the experimental diet for another 10 wk. Milk production by cows fed those diets averaged 30.9, 31.7, and 34.9 kg/d; protein averaged .90, .94, and 1.02 kg/d; and DMI averaged 16.8, 18.8, and 18.2 kg/d, respectively, for the two 10-wk periods. No dietary differences occurred for 3.5% FCM, estimated DM digestibility, BW, BW gain, or percentages of milk fat, protein, or SNF. Ruminal isobutyrate and isovalerate differed by diet; isovalerate was highest in the diet containing soybean meal plus corn gluten meal, probably because of the high Leu content of corn gluten meal. Ruminal NH3 N did not differ. The favorable production response by cows fed dried brewers grains can be explained by a more favorably balanced AA profile in the ruminally undegraded protein than in other diets.

Animal Feed↗
PubMed1977

Nutritive efficiencies of lactalbumin and wheat gluten at very low levels of intake in adult rats.

The nutritive values of proteins in relation to their intake levels were evaluated by feeding adult male rats weighing 250 g diets containing 0%, 0.39%, 0.78%, 1.56%, 2.34%, 3.90%, 7.79% and 15.58% lactalbumin or wheat gluten for three weeks. The biological values (BV) of both proteins were high at low levels of protein intake but decreased with increase in protein intake. The BV of wheat gluten was estimated to be about 100 at a level of intake of 1.56% but only 25 at a level of 15.58%. Similarly, the BV of lactalbumin decreased with increase in the protein level, being 67 at a level of 7.79%. The BVs of both proteins at low levels of dietary protein (below 2.34% of lactalbumin or 0.78% of wheat gluten) were apparently more than 100 because urinary N excretion was less than endogenous N. The BVs also decreased with time during the three-week test period. It is concluded that BV of a protein is not a fixed value but varies with the experimental conditions especially with changes in the amount of intake, and that differences in the qualities of various proteins cannot be compared quantitatively at a single level of protein. The results were briefly discussed in relation to protein requirements.

Age Factors↗
PubMed1987

Gluten content of Australian pharmaceutical products.

The treatment of patients with coeliac disease is based on the strict avoidance of gluten. Such patients may be at risk if they ingest inadvertently pharmaceutical products that contain gluten. This paper provides information on the gluten content of Australian pharmaceutical products. It is recommended that legislation be introduced to require that excipients in pharmaceutical products which are known to be potentially hazardous to susceptible individuals be listed on the product label, possibly in code form.

Australia↗
PubMed1999

[Long-term collagenous sprue--remission with a gluten-free diet].

We report on a 56-year-old caucasian female, 154 cm, 38 kg, who was admitted to the hospital in extremely poor, cachectic condition with pretibial edemas, ascites and pleural effusion with a history of severe watery diarrhea up to 15 times daily. The patient's history with permanent meteorism, recurrent episodes of watery diarrhea and dystrophy dates back to her early childhood. Multiple biopsies from the distal part of the duodenum showed a complete atrophy of villi with an extensive subepithelial layer of collagenous fibers. No antigliadin or antiendomysial antibodies could be detected. Under a strictly gluten-free diet, the patient's symptoms quickly improved. During the follow-up of one year, no recurrence of diarrhea and meteorism has been seen. The patient showed a continuous improvement of her general condition and nutritional state with a weight gain to 50 kg. Repeated control biopsies still revealed a pronounced villous atrophy. However, no subepithelial collagenous fibers were detectable. A diagnosis fitting to the patient's clinical symptoms of severe malabsorption, would be the rare "collagenous sprue", which is considered to be gluten-insensitive. However, the patient's good response to a gluten-free diet raises the possibility, that the collagenous layer represents a manifestation of more severe forms of common celiac disease. In this case, it would not be necessary to regard collagenous sprue as a distinct entity.

Biopsy↗
PubMedDate not supplied

[Sensitivity to cow's milk proteins and gluten in children's allergic disease].

Aim of the study was the evaluation of sensitivity to cow's milk and gluten participation in children's allergic disease. Our studies were carried out in a group of 191 children, aged between 7 months and 18 years, with sensitivity to cow's milk proteins and gluten in type I and/or IV according to Gell and Coombs. Sensitivity to these allergens was confirmed in 88.4% of the 216 examined children with allergic disease. We showed a prevalent frequency of allergen specific T-cell responses in mechanism IV (in type IV - 79.58%, in type I - 10.99%, in I+IV - 9.42%). Evidence is presented that sensitivity to cow's milk proteins and gluten in I and IV Gell's and Coombs' mechanisms is more likely in small children and that it decreases with age.

Adolescent↗
PubMedDate not supplied

[Study of the fractional composition of protein substances in gluten by the gel-chromatographic method].

The fractional composition of protein substances of the gluten was studied by gel chromatography. The gluten proteins from the mormal wheat flour and eurygaster damaged wheat flour were compared. The content of the high molicular weight fraction was measured for the gluten of weak and normal flour. The fractional composition of gliadin from the normal and weak flour was investigated. The molecular weight of gliadin from eurygaster damaged grain was shown to change during autolysis.

Chromatography, Gel↗
PubMed2001

Diagnosing coeliac disease by rectal gluten challenge: a prospective study based on immunopathology, computerized image analysis and logistic regression analysis.

The purpose of this study was to evaluate the use of rectal gluten challenge in the diagnosis of coeliac disease. A total of 103 patients with features suggestive of this diagnosis were prospectively enrolled into the study; a diagnosis of coeliac disease was based on strictly defined criteria used in judging the proximal jejunal biopsy. On that basis, 45 out of the 103 patients were deemed to have coeliac disease. A slurry of gluten powder in physiological saline was introduced into the rectum, and biopsies taken before and at 2 h or 4 h after the challenge were examined immunohistochemically by computerized image analysis. Cell counts were analysed by logistic regression, and the best equations were obtained for each challenge group. The 2 h challenge yielded diagnostic sensitivity and specificity of 69.6% and 78.6% respectively. The 4 h challenge provided sensitivity and specificity of 100% and 100% respectively. These results were compared with other clinical diagnostic predictors,including anti-endomysial antibodies, which yielded diagnostic sensitivity and specificity of 70% and 98% respectively. It is concluded that a 4 h rectal challenge is a highly sensitive means of identifying gluten-sensitized individuals, and would be of particular value in cases showing negative antibody screening or equivocal biopsy appearances.

Adult↗
PubMed2001

[Antibodies to tissue transglutaminase as marker of gluten-free diet maintenance in patients with coeliac disease].

The aim of the study was to assess the compatibility of IgA antiendomysium antibodies (IgAEmA) and IgA tissue transglutaminase antibodies (IgAtTG) in treated patients with coeliac disease and to estimate the value of the IgAtTG-ELISA as a marker of gluten-free diet maintenance. The study included 71 children and young adults (46 F, 25 M) aged from 6 years to 27 years, on the gluten-free diet (in some cases unrestricted) for at least 4 years because of coeliac disease diagnosed according to ESPGHAN criteria. Serum samples of all patients were examined simultaneously for IgAEmA titer by indirect immunofluorescence and IgAtTG level by ELISA test. IgAEmA antibodies were present in 26 patients (36.6%). IgAtTG-ELISA were positive in 23 patients (32.4%), equivocal--in 8 patients (11.3%) and negative--in 40 patients (53.6%). The serological tests were compatible in 58 patients (81.7%). If we consider equivocal results of IgAtTG test to be negative, IgAEmA and IgAtTG tests will be compatible in 64 cases (90.1%). Tissue transglutaminase antibodies ELISA provide a sensitive test of the gluten-free diet maintenance in patients with coeliac disease.

Adolescent↗
PubMedDate not supplied

[Cancer in children with celiac disease. Gluten-sensitive enteropathy in a boy operated for Wilms tumour].

In adults untreated coeliac disease (CD) is associated with a wide variety of malignant complications. The overall mortality in CD is approximately twice that of the general population. The excess deaths are due mainly to intestinal lymphoma. In Europe, only 26 cases of CD and cancer in children have been published. There is evidence that cancer and CD in children are underreported. We report a case of Wilms tumour in a child who developed CD. As a neonate the boy had been successfully operated for congenital mesoblastic nephroma. Gluten was introduced in his diet at age of 12 months. He developed vomiting, abdominal distention ad hypertransaminosaemia. Up to the age of 16 months he had lost 1500 g and coeliac crisis was diagnosed (IgAEMA titre 1:50 and in the duodenal biopsy specimen there was crypt hyperplastic total villous atrophy). Symptoms improved rapidly when gluten-free diet was initiated, and 4 years later, clinical and serological findings were normal. Wilms tumour accounts for 6% to 7% of all childhood malignancies. There are no reports in MEDLINE about coeliac disease in patients who have been operated for the tumour. This seemed intriguing because prevalence of gluten-sensitive enteropathy calculated for Europe varies between 1/100 and 1/300.

Antibodies, Anti-Idiotypic↗
PubMedDate not supplied

Distribution of deoxynivalenol in wheat, wheat flour, bran, and gluten, and variability associated with the test procedure.

Analytical data obtained on deoxynivalenol (DON) concentration in naturally contaminated wheat during processing in an industrial mill were statistically analyzed, and the distribution functions of DON concentration in lots of wheat, bran, wheat flour, and gluten were estimated. The analytical method had acceptable precision (HORRAT 0.25-0.32) for each test sample. The total variance combined sampling, sample preparation, and analytical variances were 0.188, 0.033, 0.42, and 0.0014 ppm2 for wheat, 1.93; flour, 0.99; bran, 4.68; and gluten, 0.29, respectively. The distribution function of DON contamination presented an asymmetric tail for high values of concentration in wheat grains and wheat flour; in bran it seemed to be bimodal with 2 separated peaks of different concentrations; in gluten the normal distribution function gave a reasonably good fit to empirical data. The function eta(c) = -In(-Inp), where p (c) is the cumulative distribution function was linear with c in the so-called extreme-value type I distribution and could be fitted by a cubic polynomial in c in the distributions determined for all the products. This variability and distributional information contributes to the design of better sampling plans in order to reduce the total variability and to estimate errors in the evaluation of DON concentration in lots of wheat and wheat products.

Dietary Fiber↗
PubMed2003

[Effects of different environments on qualitative characters of three gluten wheat cultivars].

Six cultivars including strong, middle and weak gluten wheat varieties were cultivated at five latitudes(from 32 degrees N to 36 degrees N) in Henan Province, and the variations of their qualitative characters were analyzed in relation to the different environments. The result indicated that latitudes had great effects on the protein content, wet gluten content, softening degree, evaluation value, and extensibility of the cultivars. The rheological parameters measured by farinogram and the extensogram were significantly different among different among different cultivars, and the coefficients of variation were quite large. The variations in qualitative characters among cultivars and among latitudes were different. With the rising latitude, the protein content, wet gluten content, development time, evaluation value, extensibility, resistance, and maximum resistance showed an increase tendency, with a few exceptions for certain latitude. For most qualitative characters, there was a clear boundary between Xinyan(32 degrees N) and Zhumadian(33 degrees N). According to these results, suggestions in the latitude layout of the hi-quality wheat cultivars and in improving their cultuvation practices were made.

Crops, Agricultural↗