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Fertility of ewe lambs maintained indoors year-round on an accelerated breeding program.

Data from records of 330 crossbred ewe lambs mated to 142 rams over 11 breeding periods were analyzed for lambing percentage to one service. These ewe lambs in two separate flocks were reared and maintained indoors year-round and were derived from the three synthetic crossbred strains being developed at the Animal Research Centre in Ottawa, Ontario, Canada. At first exposure to rams, the ewes were 6 to 7 mo of age and had no hormonal treatments to induce or synchronize estrus. The two flocks were bred alternately in January, May or September to establish an 8 mo breeding cycle. The rams were at least 10 mo old and were maintained in one of two lighting regimens. Fifteen percent of the ewes lambed and the average prolificacy was 1.6. It appears that ewe lambs mated to rams maintained in a constant day length (10 h light: 14 h dark/24 h) environment had lower fertility than those ewe lambs mated to rams maintained in an environment such that the day length was altered between 4 mo of 10 h light: 14 h dark/24 h and 4 mo of 18 h light: 6 h dark/24 h. Although the fertility level was low, the data suggest that the breeding of ewes 6 to 7 mo of age is feasible.

Animal Husbandry↗

Serving capacity of crossbred yearling beef bulls. I. Single-sire mating behavior and fertility during average and heavy mating loads at pasture.

Eighty crossbred, virgin, yearling beef bulls were subjected to three serving capacity (SC) tests. Ten low SC (LSC) and 10 high SC (HSC) bulls similar in weight (avg wt = 452 kg), testicular size and seminal traits but differing in average services were selected. Objectives were to evaluate the mating behavior and fertility of LSC and HSC bulls at pasture when exposed to an average and a heavy mating load and to determine the effect of sexual activity on body weight and testicular characteristics. One LSC and one HSC bull per block were exposed single-sire to 25 naturally cyclic (N) cows for 3 d and to 9 estrus-synchronized (S) cows for 1 d in a randomized complete block design consisting of 10, 4-d blocks. Bulls were fitted with pedometers to record distance travelled. Behavioral data were collected by periodic visual observation. During the N cow treatment, LSC bulls had fewer (P less than .05) services per cow, total services and a higher mount to service ratio than HSC bulls. During the S cow treatment, LSC bulls showed increased sexual activity, though they achieved fewer (P less than .09) services per cow. Pregnancy rates for LSC and HSC bulls did not differ (P greater than .20) and across SC groups averaged 53.6% for N cows and 31.9% for S cows. Distance travelled also was similar (P greater than .20) between SC groups for both treatments. Sexual activity during pasture exposure had no effect (P greater than .20) on testicular characteristics but resulted in an average decline in body weight of 25.7 kg across SC groups during the 4-d period. Post-exposure SC tests found that LSC bulls had increased (P less than .05) services, decreased mounts and mount to service ratio compared to pre-exposure measurements, but LSC were still lower (P = .06) than HSC bulls for average services. These results suggest that LSC virgin, yearling bulls should be offered sexual experience and retested before their inherent SC can be determined.

Animals↗

Serving capacity of crossbred yearling beef bulls. II. Summer grazing activity and body temperature patterns during average and heavy mating loads at pasture.

Ten low (LSC) and 10 high (HSC) serving capacity yearling bulls were exposed individually to 25 naturally cyclic (N) cows for 3 d (average mating load) and subsequently to 9 estrus-synchronized (S) cows for 1 d (heavy mating load) in a randomized complete block design consisting of 10, 4-d blocks. Bulls were fitted with vibracorders and temperature acquisition modules to record grazing activity and body temperature (BT), respectively. During the N cow treatment, LSC bulls had fewer (P less than .05) services per cow and a higher mount to service ratio than HSC bulls, and LSC bulls tended (P = .12) to graze less total time than HSC bulls (7.8 vs 9.0 h/d, respectively). However, both groups of bulls exhibited similar diurnal grazing patterns with two major daily grazing periods; the first (0400 to 1300) peaked early in the morning (0600) and the second (1700 to 2200) occurred in late afternoon and evening. During the S cow treatment, LSC and HSC bulls did not differ (P = .60) in grazing time or pattern, but similar mating activity was exhibited by both SC groups while exposed to S cows. Bulls grazed little during the hottest part of the day (1300 to 1700), and LSC and HSC bulls spent only 2.2 and 2.4 h, respectively, grazing during dark, cooler periods near midnight. Body temperature did not differ between SC groups and averaged 39.3 degrees C during N cow treatment and 39.4 degrees C during S cow treatment. Body temperature had a distinct, monophasic diurnal pattern in bulls exposed to an average mating load.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Selection for milk production from a lactation biology viewpoint.

The success of selection for increased milk production in dairy cows is apparent. Certainly, many herds now have average production levels that would have only been associated with the best producers in the herd 30 yr ago. There are, of course, many reasons for this success. Among these are improvements in genetic selection methods and associated use of artificial insemination, better fulfillment of nutritional needs and diet formulation, and careful attention to mastitis control and milking management. Development of new management tools (i.e., bovine somatotropin, improved crops, estrus detection devices, estrus synchronization, monitoring of individual animal performance, and disease prevention) should not be forgotten. Although many aspects of a dairy operation determine overall performance and profitability, the focus of this paper is the udder. Information indicates that both the structure and function of the bovine mammary gland have been directly impacted by long-term selection for increased milk production but improved functionality may have been more important. This review also considered studies that attempt to develop techniques and measurements for possible selection of genetically superior animals including measurement of circulating hormones and direct assay of mammary tissue function.

Animals↗

An alternative AI breeding protocol for dairy cows exposed to elevated ambient temperatures before or after calving or both.

Our objective was to determine if a timed artificial insemination (AI) protocol (Ovsynch) might produce greater pregnancy rates than AI after a synchronized, detected estrus during summer. Lactating Holstein cows (n = 425) were grouped into breeding clusters and then assigned randomly to each of two protocols for AI between 50 and 70 days in milk. All cows were treated with GnRH followed 7 d later by PGF2alpha. Ovsynch cows then were treated with a second injection of GnRH 48 h after PGF2alpha and inseminated 16 to 19 h later. Controls received no further treatment after PGF2alpha and were inseminated after detected estrus. Pregnancy was diagnosed once by transrectal ultrasonography (27 to 30 d after AI) and again by palpation (40 to 50 d). Based on concentrations of progesterone in blood collected before each hormonal injection, only 85.4% of 425 cows were considered to be cycling. Although conception rates were not different between protocols at d 27 to 30, AI submission rates and pregnancy rates were greater after Ovsynch (timed AI) than after detected estrus. A temperature-humidity index > or = 72 was associated with fewer controls detected in estrus with lower conception than for controls detected in estrus when index values were < 72, whereas the reverse was true for cows after the Ovsynch protocol. We concluded that a timed AI protocol increased pregnancy rates at d 27 to 30 because its success was independent of either expression or detection of estrus. However, because of poorer embryonic survival in Ovsynch cows during heat stress only (39.5 vs. 69.2% survival for Ovsynch and control, respectively), pregnancy rates were not different by d 40 to 50 after timed AI.

Animals↗

Reproductive performance and milk production of Assaf sheep in an intensive management system.

The Assaf breed of dairy sheep, a stabilized cross of the Awassi and East Friesian breeds, has replaced the Awassi as the breed of choice in its country of origin, Israel, and has spread to other Mediterranean countries. In Israel the Assaf breed is managed under an intensive production system involving weaning lambs at birth, rearing them artificially, and milking ewes after parturition. There are several breeding periods in the year when ewes are mated following hormonally synchronized estrus. Records of 18,976 lactations from 5 farms were analyzed to investigate factors that influenced Assaf milk and reproductive performance. Lactation curves were fitted to each lactation, and a range of parameters and calculated values were analyzed. Daily milk yield records also were analyzed to describe a typical Assaf lactation and compared with those of the Awassi breed. Factors affecting age at first lambing also were studied. An average Assaf ewe kept under this intensive management regimen was found to produce 334 L of milk during a 173-d lactation. Mean litter size was 1.57 lambs/ewe lambing, and lambing interval was 272 d. Milk production was affected by litter size, with twin- and triplet-bearing ewes producing approximately 20 L more milk per lactation than single-bearing ewes. Day length was the major environmental variable influencing milk yield. The difference between midsummer and midwinter day lengths accounted for a difference in daily milk yield of 0.44 L in favor of summer. Ewe lambs that were mated for the first time at later ages produced more lambs and more milk due to greater early lactation characteristics. Milk production was found to be negatively associated with subsequent reproductive performance. Comparing these results with those from an earlier study in the Awassi breed, the Assaf was found to produce less milk during a shorter lactation than the Awassi, but its greater litter size made it a more profitable breed.

Age Factors↗

Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows.

In recent years, the number of researchers interested in mammary development and mammary function in dairy animals has declined. More importantly this cadre of workers has come to rely more than ever on scientists focused on and funded by breast cancer interests to provide fundamental mechanistic and basic cellular insights. Philosophically and practically this is a risky path to better understand, manipulate, and control a national resource as important as the dairy cow. The efficiency, resourcefulness, and dedication of dairy scientists have mirrored the actions of many dairy producers but there are limits. Many of the applications of research, use of bovine somatotropin, management of transition cows, estrus synchronization techniques, and so on, are based on decades-old scientific principles. Specific to dairy, do rodents or breast cancer cell lines adequately represent the dairy cow? Will these results inspire the next series of lactation-related dairy improvements? These are key unanswered questions. Study of the classic mammogenic and lactogenic hormones has served dairy scientists well. But there is an exciting, and bewildering universe of growth factors, transcription factors, receptors, intracellular signaling intermediates, and extracellular molecules that must ultimately interact to determine the size of the mature udder and the functional capacity of mammary gland in the lactating cow. We can only hope that enough scientific, fiscal, and resource scraps fall from the biomedical research banquet table to allow dairy-focused mammary gland research to continue.

Animals↗

Protein intake and development of ovarian follicles and embryos of superovulated nonlactating dairy cows.

Nonlactating Holstein cows (n = 12) were assigned randomly to one of two diets (approximately 30% concentrate) in a crossover design. Urea and soybean meal were used to increase CP content of diet from 12.3 to 27.4%. Mean concentrations of plasma urea were 9.8 and 21.3 mg/dl for respective diets. After 35 d on diets, cows were brought to a synchronized estrus (progestin implant plus PGF2 alpha injection). From d 10.5 to 14.5 following estrus, cows received i.m. injections of decreasing doses of FSH to initiate superovulation. On d 3 of FSH treatment, PGF2 alpha was injected with FSH. Cows were bred twice by AI when detected in estrus. Embryos were collected nonsurgically 6.5 d after breeding, and quality was assessed using visual, microscopic, and staining techniques. Development of follicles throughout this period was monitored using ultrasonography. Using ultrasonography, no differences were detected in the number or percentages of preovulatory, anovulatory, and ovulatory follicles induced during superovulation. Number of recovered embryos averaged eight per cow. Numbers and percentages of normal embryos, abnormal or retarded embryos, and unfertilized ova were similar between diets, as were the numbers and percentages of transferable and nontransferable embryos. Excess intake of CP failed to affect the growth or number of ovarian follicles and health and number of embryos of energy adequate, nonlactating dairy cows.

Ammonia↗

The effect of intrauterine inoculation with Ureaplasma diversum on bovine fertility.

To determine the influence of Ureaplasma diversum on bovine fertility 11 uninfected virgin heifers with normal ovarian cyclic activity were randomly allocated to test or control groups. At a synchronized estrus, five test heifers were given an intrauterine broth inoculum containing 1.09 x 10(8) to 1.4 x 10(9) colony forming units of U. diversum and six control animals were infused with sterile ureaplasma broth medium. All animals were artificially inseminated within one hour of infusion. Pregnancy was diagnosed in one of five test heifers and all of six controls by serum progesterone concentrations measured to 25 days postinsemination. The difference in pregnancy rates between the two groups was statistically significant (p = 0.0152). It was concluded that under the conditions of this experiment U. diversum is capable of causing infertility in cattle.

Animals↗

[Transplantation of embryos in sheep breeding].

Intra- and inter-breed transplantations were carried out with Bulgarian fresh embryos and with frozen embryos imported from Poland. A complex biotechnology was worked out, including methods of estrus synchronization, of inducing superovulation, and of producing, morphologic evaluation, storing, and transplantation of sheep embryos. Discussed are the possibilities of using embryo transplantation in sheep breeding for the fuller use of the reproductive potential of elite ewes as donors of embryos, for the introduction of new genotypes by means of importing frozen embryos as well as a modern model for fundamental investigations in the reproductive and cell biology.

Animals↗

Comparison of palpable corpora lutea with serum progesterone concentrations in cows.

Serum progesterone concentrations were used to evaluate rectal palpation of corpora lutea as a method for assignment of postpartum beef cows to prostaglandin treatment and nontreatment groups. On the basis of 124 evaluations, 18% of the cows were assigned incorrectly to the treatment group and 37% of the cows were assigned incorrectly to the nontreatment group. The inability of palpators to accurately select cows with a mature corpus luteum may diminish the success of estrus synchronization regimens that use rectal palpation of corpora lutea for selection of cows for prostaglandin therapy.

Animals↗

A study on the effect of Pseudomonas aeruginosa in semen on bovine fertility.

Two experiments were done to demonstrate whether the presence of Pseudomonas aeruginosa in bovine semen could affect fertilization and/or early embryonic development. In the first experiment, superovulated heifers were inseminated with semen naturally contaminated with P. aeruginosa (ADRI 102) or clean semen and seven day-old embryos were collected nonsurgically. The endometrium of treated heifers appeared more sensitive to the flush procedures. In experiment 2, heifers were inseminated at synchronized estrus with semen experimentally contaminated with P. aeruginosa (ADRI 102) and processed in the same way as commercial semen with antibiotics (gentamicin, lincomycin, spectinomycin and tylosin) or processed without antibiotics added. Embryos were recovered at slaughter seven days later. In general, there was no significant reduction in fertility or development of embryos in vitro as a result of relatively high numbers of P. aeruginosa in bovine semen.

Animals↗

Bioequivalency comparison between two gonadotropin-releasing hormone products.

The bioequivalency of 2 gondatropin-releasing hormone (GnRH) preparations, gonadorelin diacetate tetrahydrate and gonadorelin semicarbonate, was compared on the basis of luteinizing hormone (LH)-releasing ability of the 2 products in diestrous dairy cows. Twenty-four cycling, nonlactating Holstein cows were subjected to a double prostaglandin estrus synchronization treatment to simultaneously control stage of the estrous cycle and time factors as potential variables effecting LH responses to the treatments being studied. Circulating progesterone concentration was determined to verify stage of cycle at strategic times throughout the study. Twelve days after the second prostaglandin treatment, all cows were randomly assigned to 1 of 2 groups (n = 12). Each group of 12 cows received single doses (100 micrograms) of either GnRH preparation at the start of each test period in a 2-period crossover design. Serum samples were obtained prior to and at 12 times (10, 20, 30, 45, 60, 90, 120, 180, 240, 360, 480, and 1,440 minutes) after treatment and were assayed to determine circulating LH concentration. Significant difference between the 2 GnRH products was not found with respect to: mean concentration of LH in the blood during the 24 hours after treatment; maximal LH concentration; time from treatment to maximal LH concentration; and area under the LH concentration curve from time 0 through each of 7 times after treatment (0.5, 1, 1.5, 2, 4, 8, and 24 hours). These data confirm the bioequivalency of the 2 GnRH products.

Animals↗

Safety assessment of moxidectin 1% injectable on reproductive performance in beef cows.

The safety of moxidectin 1% injectable anthelmintic (0.6 mg/kg of body weight, 3 times the recommended dose) was evaluated in 145 reproductively sound, beef cows undergoing estrous cycle. Five treatment groups received moxidectin 1% injectable at specific times relative to a synchronized estrus (day 0): preovulatory treatment (day -2, treatment group 1), treatment at ovulation (day 0, group 2), and treatment after ovulation (days 7, 14, and 28, group 3, 4, and 5, respectively). Two groups of control cows received an injection of vehicle only at times corresponding to treatment in the other groups (6 at days -2, 7, and 28; 7 at days 0, 7, and 14). A final control group (8) received neither product or vehicle. Adverse clinical reactions were not observed in moxidectin- or vehicle-treated cows. Cows were bred by artificial insemination between days -2 and 25 and, subsequently, by breeding-sound bulls through day 65 of the study. Treatment and control groups did not differ in pregnancy rate or time to conception. Moxidectin (at 3 times the therapeutic dose) did not have deleterious effects on cow reproductive performance as examined (eg, at folliculogenesis, ovulation, and the early embryonic phase of development).

Analysis of Variance↗

Comparison of diagnostic methods for detection of active infection with Tritrichomonas foetus in beef heifers.

OBJECTIVE: To compare sensitivity of a generic trypticase-yeast extract-maltose (TYM) medium versus a commercial nutrient medium in the diagnosis of Tritrichomonas foetus infection in heifers and to assess sensitivity when incubation of samples inoculated into commercial medium pouches is delayed overnight. DESIGN: Prospective study. ANIMALS: 30 virgin beef heifers. PROCEDURES: 20 heifers vaccinated with a trichomonad antigen and 10 unvaccinated control heifers were exposed at synchronized estrus by intravaginal instillation of 10(6) T foetus organisms. Cervicovaginal mucus samples were collected every other week for 10 weeks from controls and once (10 weeks after exposure) from vaccinated heifers. Samples were inoculated into both media and immediately incubated at 37 C (98.6 F). A duplicate inoculation from controls was made into commercial medium, and the pouch was shipped overnight to a diagnostic laboratory without prior incubation. RESULTS: For 40 of 50 samples from control heifers, there was agreement on diagnoses between media. There was agreement on a positive diagnosis for 3 of 20 samples from vaccinated heifers and on a negative diagnosis for 15 of these 20 samples. For samples shipped overnight before incubation, there were 10% fewer positive diagnoses, compared with samples incubated immediately in commercial medium and 10% more positive diagnoses, compared with samples immediately incubated in TYM. CLINICAL IMPLICATIONS: Use of the commercial medium is a more sensitive indicator of current infection in heifers than use of generic TYM medium. In herds where infection prevalence is high, this method is likely to identify more infected females, an important consideration when control programs include culling of infected cows.

Animals↗

Methods of identifying and inseminating nonpregnant beef females after synchronization of second estrus with norgestomet implants.

Beef females (547) were included in three experiments to evaluate methods of identifying and inseminating nonpregnant beef females after synchronization of second estrus with norgestomet implants. In the first experiment, heifers not pregnant to the first insemination were identified for insemination via estrus (inseminated via the a.m./p.m. rule or 48 h after implant removal). In the second experiment, females not pregnant to the first insemination were identified for insemination via estrus (inseminated via the a.m./p.m. rule) or progesterone concentrations < 1.5 ng/mL at implant removal (inseminated 48 h after implant removal). In the third experiment, heifers not pregnant to the first insemination were identified for insemination via progesterone concentrations (as in experiment 2) or anterior vagina electrical resistance values < 81 ohm resistance 48 h after implant removal (inseminated after resistance measured). All methods of identifying and inseminating nonpregnant females were equally effective (P > 0.10) and did not effect (P > 0.10) calving rates from the first and second AI.

Journal Article↗

Short synchronization system for estrus cycles in dairy heifers: a preliminary report.

In previous studies we demonstrated that the administration of a luteolytic dose of cloprostenol to dairy cows in luteal phase, followed by hCG plus estradiol benzoate (EB) 12 h later, led to successful timed AI 48 h after the initiation of treatment. This article reports two consecutive studies. In Study 1 we determined the pregnancy rate of dairy heifers in luteal phase (established by palpation per rectum) treated with cloprostenol followed by 250 IU of hCG plus 1 mg of EB 12 h later, and inseminated 48 h after cloprostenol injection. Study 2 was designed to evaluate the efficiency this synchronization protocol, irrespective of the estrus stage of the animals. In Study 1, 1272 Friesian heifers aged 14 to 16 months with a palpable corpus luteum received 500 mcg cloprostenol. Heifers were then synchronized either according to the hCG plus EB protocol (hCG-EB, n=637), or by a second dose of cloprostenol 11 d later (PG, n=636). Animals in this last group served as controls and were inseminated 72 and 96 h after the second cloprostenol injection. The pregnancy rate was significantly higher (P<0.0001) in the hCG-EB group (59.5%, 379/637) than in PG (44.8%, 285/636). In Study 2, 135 contemporary heifers (with no corresponding information on estrus stage) were subjected to the same protocol as those in the hCG-EB group of Study 1. These animals were classified in retrospect according to estrus stage established by plasma progesterone concentration. Pregnancy rates were 66.7% (24/36), 51% (25/49) and 58% (29/50) for animals in the follicular, early/late luteal, and mid-luteal phase, respectively. The total pregnancy rate was 57.8% (78/135). These findings indicate an improved pregnancy rate for heifers subjected to single insemination after cloprostenol/hCG/EB synchronization, compared to double insemination after synchronization by 2 cloprostenol injections 11 d apart. The cloprostenol/hCG/EB protocol did result in acceptable pregnancy rates after timed AI of dairy heifers regardless of their estrus cycle phase.

Animals↗

Intervals from norgestomet withdrawal and injection of equine chorionic gonadotropin or P.G. 600 to estrus and ovulation in ewes.

Synchronization of estrus and ovulation is essential for AI of ewes during a predetermined time frame, and progestogen-eCG treatments are typically used to prepare the ewes. However, eCG is not readily available in the United States, but P.G. 600 (400 IU of eCG and 200 IU of hCG) is available. Thus, we conducted a study to determine the effects of eCG and P.G. 600 on the timing of estrus and ovulation after progestogen withdrawal. Ewes were assigned to two replicates of an experiment with the following treatments: 1) 3-mg norgestomet implant (i.e., one-half of a Syncro-Mate-B [SMB] implant) for 10 d, plus 2 mL of saline i.m. at SMB removal (n = 11); 2) 3-mg SMB implant for 10 d, plus 400 IU of eCG i.m. at SMB removal (n = 13); and 3) 3-mg SMB implant for 10 d, plus P.G. 600 i.m. at implant removal (n = 9). On d 6 after SMB insertion, PGF2alpha was used to induce luteolysis. Beginning 12 h after implant removal, vasectomized rams were used at 12-h intervals to check for estrus. When a ewe was detected in estrus, each ovary was evaluated ultrasonically. Ovaries were evaluated again 16 h later and then at 8-h intervals until ovulation. Treatment altered the interval from implant removal to estrus (less [P < 0.05] in SMB + eCG than in the other two groups) and to ovulation (greatest [P < 0.05] in SMB). However, the treatment x replicate interaction was significant for the intervals from implant removal to estrus (P < 0.03) and from implant removal to ovulation (P < 0.05). An inconsistent response in the SMB-treated ewes seemed to be primarily responsible for the interaction. The intervals to estrus and to ovulation for the SMB-treated ewes were shorter (P < 0.05) in Replicate 1 than in Replicate 2. Also, both intervals seemed to be less consistent between replicates for the SMB + P.G. 600- than for the SMB + eCG-treated ewes; that is, eCG seemed to increase the predictability of the intervals to estrus and to ovulation. Neither the main effects of treatment and replicate nor their interaction were significant for the interval from estrus to ovulation (38.4 /- 3.3 h), size of the ovulatory follicle (7.7 +/- 0.8 mm), or ovulation rate (1.6 +/- 0.2). We concluded from this experiment that eCG is a better choice than P.G. 600 as the gonadotropin to use at the time of progestogen withdrawal to prepare ewes for AI during a predetermined interval.

Animals↗