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Complement fixation test for the diagnosis of Lyme disease.

Sera from 43 patients were tested for complement-fixing antibodies to Borrelia burgdorferi; these patients included 8 with confirmed Lyme disease, 21 who were serologically positive but not likely to have Lyme disease, and 14 who were serologically negative. Seven individuals, all confirmed Lyme disease patients, had complement-fixing antibodies. Complement fixation may be a useful confirmatory test for Lyme disease.

Antibodies, Bacterial↗

Automated complement fixation test for the detection of antibodies against the core of hepatitis B virus (HBc).

The automated complement fixation system presented in this paper differs from those described by Studievic et al. (1971) and Vargues and Henley (1974). In the one developed here only one sampler is used with a wheel presenting a double row of cups. This system can assay 60 samples per h and can be modified to double this number. Our unit requires an incubation period of 15 min at 37 degrees C and we have nerver observed any contamination of the samples during the assay run.

Antibodies, Viral↗