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Effects of supplementing prairie hay with corn and soybean meal on intake, digestion, and ruminal measurements by beef steers.

Prairie hay supplemented with various amounts of corn and soybean meal was fed to steers in two experiments. Effects of supplementation on hay OM intake, digestion, and ruminal fermentation and kinetics were measured. A preliminary study was conducted to attain accurate values for OM intake and digestibility of prairie hay to be used in ration formulation using the NRC (1996) level 1 model. Ten steers (284 +/- 9 kg) given ad libitum access to chopped prairie hay (75% NDF, 6% CP) were supplemented with dry-rolled corn (0.75% of BW/d) plus soybean meal (0.25% of BW/d). Hay OM intake was 1.85% of BW and hay OM digestibility was 48%. Based on results from the preliminary study, eight ruminally cannulated beef steers (317 +/- 25 kg) received a sequence of eight different supplementation combinations (2 x 4 factorial arrangement of treatments). These supplements consisted of dry-rolled corn at either 0 or 0.75% of BW (DM basis) daily combined with one of four amounts of added soybean meal to provide between 0 and 1.3 g of degradable intake protein (DIP)/kg of BW. After supplements had been fed for 10 d, feces were collected for 4 d. Intake of hay and total OM increased quadratically (P < 0.01) in response to added DIP with or without supplemental corn. Hay OM digestibility increased quadratically (P = 0.03) as DIP was added when corn was fed in the supplement. Intake of digestible OM was greater (P < 0.01) with than without corn supplementation. Increasing DIP increased (P < 0.01) digestible OM intake regardless of whether corn was fed. Inadequate ruminally degraded protein in grain-based supplements decreased forage intake, digestibility, and energy intake of cattle fed low-quality prairie hay. Providing adequate supplemental DIP to meet total diet DIP needs seemed to overcome negative associative effects typically found from supplementing low-quality forages with large quantities of low-protein, high-starch feeds.

Animal Feed↗

Flaking corn: processing mechanics, quality standards, and impacts on energy availability and performance of feedlot cattle.

Based on performance of feedlot cattle, steam flaking increases the value of corn by 18%, considerably more than is suggested by tabular values. Tabular values underestimate the energy availability of flaked corn by failing to account for digestibility of the nonstarch OM that is increased by flaking by the same magnitude (10%) as starch. Correcting for improvement in digestibility of nonstarch OM increases the NEg value of steam-flaked corn to 1.70 Mcal/kg, a value very close to values calculated from cattle performance trials. Digestibility of starch from corn grain is limited by the protein matrix that encapsulates starch granules, and by the compact nature of the starch itself. Disruption of the protein matrix (by shear forces on hot grain during flaking) is the first limiting step toward optimizing starch digestion. Five critical production factors influence the quality of steam-flaked corn: steam chest temperature, steaming time, roll corrugation, roll gap, and roll tension. For optimal shear, it is important that rolls be hot and that kernels be hot when flaked. Steam chests should be designed to allow a steaming time of at least 30 min at maximum roller mill capacity producing a flake of 0.31 kg/L (24 lb/bushel). As little as 5% moisture uptake during steaming appears adequate. The rate of flaking and distribution of kernels across the rolls also are critical. Quality standards for steam-flaked corn include measurements of flake thickness, flake density, starch solubility, and enzyme reactivity. Flake density, the most common quality standard, closely associated with starch solubility (r2 = 0.87) and enzyme reactivity (r2 = 0.79), still explains only 63% of the variability in percentage fecal starch and 52% of the variability in starch digestibility. Direct determination of fecal starch can explain 91% of the variability in starch digestion. The NEg value of corn can be predicted from fecal starch: NEg= 1.78 - 0.0184FS. Starch digestion is a Kappa Curve function of hot flake density, reaching a maximum at a flake density of approximately 0.31 kg/L. Flaking to a density of less than 0.31 kg/L, though increasing starch solubility, may reduce DMI, increase variability of weight gain among animals within a pen, and predispose cattle to acidosis and bloat without increasing starch digestion. We recommend that the steam-flaking process be optimized on the basis of fecal starch analysis.

Animal Feed↗

Nutritional value of a corn containing a glutamate dehydrogenase gene for growing pigs.

Eight female PIC Line 42 pigs (initial BW = 47.5 +/- 1.8 kg) were used in a two-period switchback design (n = 4 per treatment per period) to evaluate the nutritional difference between a genetically modified corn and a similar nontransgenic corn. The genetically altered corn (gdhA+) contained a glutamate dehydrogenase gene isolated from Escherichia coli. The non-transgenic corn was the same variety lacking the transgenic cassette, grown at the same two locations. Pigs were surgically fitted with steered ileocecal valve cannulas for collection of ileal digesta. Diets were made up of primarily one of the two corn sources. Dietary AA profiles were adjusted using crystalline AA to match Illinois Ideal Protein Ratios. Pigs were limit-fed at 8% of metabolic body weight (BW0.75) in two equal feedings at 0600 and 1800 daily throughout the experiment. The study consisted of two 15-d periods. Each period consisted of a 7-d acclimation period, a 3-d total collection of feces and urine, two 12-h ileal collections, and a 3-d adjustment period between ileal collections to ensure adequate hydration. Crude protein, leucine, methionine, alanine, aspartic acid, glutamic acid, and tyrosine concentrations were greater (P < 0.05) in the gdhA+ corn than in the nontransgenic variety. The presence of the gene did not alter (P > 0.17) BW gain. Similarly, DM digestibility, fecal N excretion (grams per day), apparent total-tract N digestibility, N balance, net protein utilization, and N retained as percentages of absorbed were not affected (P > or = 0.32) by the gene modification. Apparent ileal AA digestibility values did not differ (P > 0.31) between the two dietary treatments. Results of this study suggest corn that contains the E coli. gene for glutamate dehydrogenase was nutritionally equivalent to the unaltered variety.

Animal Feed↗

Glycemic index of cracked corn, oat groats and rolled barley in horses.

Muscle glycogen synthesis depends on glucose availability. This study was undertaken to determine the glycemic and insulinemic response of horses to equal amounts of hydrolyzable carbohydrates (starch and sugar) in the form of one of three grain meals or intragastric administration of a glucose solution. In a randomized crossover design, seven horses were fed each of three grain meals (cracked corn, steamed oat groats, or rolled barley) or were infused intragastrically with glucose solution at 2 g of hydrolyzable carbohydrate (starch plus sugar) per kilogram of BW. The quantity of hydrolyzable carbohydrate ingested was not different among all treatments (P = 0.70). Plasma glucose concentration peaked in all four treatments by 1.5 to 2 h after feeding. Plasma glucose concentration remained higher than baseline in oat groats or barley-fed horses throughout 8 h, whereas plasma glucose returned to baseline by 5 to 6 h in corn-fed horses or after glucose administration. Meal consumption was slower in oat groats-fed horses than in corn-fed ones, which may confound the glycemic and insulinemic responses observed after grain feeding. Plasma glucose area under the curve (AUC) was 63% both in corn and oat groats and 57% in barley-fed horses compared with that of horses administered glucose (P = 0.13). Serum immunoreactive insulin concentration peaked between 2 and 3 h after feeding or glucose administration, and barley-fed horses had lower serum immunoreactive insulin concentration by 3 to 4 h than corn-fed horses or after glucose administration (P < 0.05). We conclude, in horses, ingestion of oat groats, corn, and barley result in similar plasma glucose AUC and, compared with the glycemic index of 100 as the glucose reference, corn, oat groats, and barley had a glycemic index of approximately 60.

Animal Feed↗

Fatty acid indices of stearoyl-CoA desaturase do not reflect actual stearoyl-CoA desaturase enzyme activities in adipose tissues of beef steers finished with corn-, flaxseed-, or sorghum-based diets.

We hypothesized that stearoyl-CoA desaturase (SCD) enzyme activity would not correlate with fatty acid indices of SCD activity in steers fed different grains. Forty-five Angus steers (358 +/- 26 kg BW) were individually fed for 107 d diets differing in whole cottonseed (WCS) supplementation (0, 5, or 15% of DM) and grain source (rolled corn, flaxseed plus rolled corn, or ground sorghum grain) in a 3 x 3 factorial arrangement. Flaxseed- and corn-fed steers had greater (P < 0.01) G:F (0.119 and 0.108, respectively) than sorghum-fed steers (0.093). Marbling score was decreased by WCS (P = 0.04), and LM area was decreased (P < 0.01) by sorghum. Plasma 14:0, 16:0, 16:1n-7, and 18:2n-6 were greatest in corn-fed steers, whereas plasma 18:3n-3 and 20:5n-3 were greatest in the flax-seed-fed steers (P < 0.01). Plasma 18:1trans-11 was least in sorghum-fed steers, and plasma cis-9,trans-11 CLA was barely detectable, in spite of high intestinal mucosal SCD enzyme activity (118 to 141 nmol*g tissue(-1).7 min(-1)). Interfascicular (i.f.) and s.c. cis-9,trans-11 CLA remained unchanged (P > or = 0.25) by treatment, although 18:1trans-11 was increased (P < or = 0.02) in steers fed corn or flaxseed. Steers fed flaxseed also had greater (P < 0.01) i.f. and s.c. concentrations of 18:3n-3 than steers fed the other grain sources. Oleic acid (18:1n-9) was least and total SFA were greatest (P < 0.01) in i.f. adipose tissue of steers fed 15% WCS. Lipogenesis from acetate in s.c. adipose tissue was greater (P < 0.01) in flaxseed-fed steers than in the corn- or sorghum-fed steers. Steers fed flaxseed or corn had larger i.f. mean adipocyte volumes (P < 0.01) than those fed sorghum and tended (P = 0.07) to have larger s.c. adipocyte volumes. Several fatty acid indices of SCD enzyme activity were decreased (P < or = 0.03) by WCS in i.f. adipose tissue, including the 18:2cis-9,trans-11/ 18:1trans-11 ratio. The 18:2cis-9,trans-11/18:1trans-11 ratio also tended to be decreased (P = 0.09) in s.c. adipose tissue by flaxseed; however, SCD enzyme activities in i.f. and s.c. adipose tissue were not affected by dietary WCS (P > or = 0.47) or grain source (P > or = 0.37). Differences in SFA seemed to be independent of SCD enzyme activity in both adipose tissues, suggesting that duodenal concentrations of fatty acids were more important in determining tissue fatty acid concentrations than endogenous desaturation by SCD.

Adipocytes↗

Ammonia and urea in corn silage-based complete mixed diets for dairy cows.

Source of supplemental N was evaluated in three corn silage-based complete mixed diets (CMD) fed to lactating dairy cows. Diets were formulated to be isonitrogenous and contained 60% corn silage and 40% concentrate on a dry matter basis. Diets were: CMD-A, ammoniated corn silage (ammonia provided 16% of total N) plus concentrate; CMD-U, untreated corn silage and concentrate (16% of total N from urea) and CMD-S, untreated corn silage plus concentrate with soybean meal. Ammoniation at 1.0% of dry weight increased crude protein content of silage from 8.5 to 12.7%. Daily means for cows fed CMD-A, CMD-U and CMD-S were: 24.2, 24.3 and 24.5 kg of fat-corrected milk; 3.17, 3.10 and 3.15% milk protein; 3.41, 3.67 and 3.63% milk fat and 3.02, 2.99 and 3.02 kg intake of total dry matter/100 kg body weight. Differences were not significant except for reduced milk fat percentage for cows fed the ammoniated corn silage diet. Both anhydrous ammonia and urea at 16% of total dietary N were found to be practical and economical substitutes for part of the supplemental soy protein. When compared to CMD with all soybean protein, greater savings in feed costs resulted from inclusion of ammoniated corn silage than with inclusion of urea.

Ammonia↗

Effects of supplemental Bermuda grass hay or corn on intake, digestion and performance of cattle consuming endophyte-infected fescue.

Effects of supplemental Bermuda grass hay (BG) or ground corn on intake, digestion and performance of cattle consuming endophyte-infected fescue (I) were studied. In Exp. 1, a Latin square study, five growing Holstein steers (158.1 kg) consumed I ad libitum and were offered 0, .3, .6, .9 or 1.2% body weight (BW) of BG daily. Total dry matter (DM) intake rose linearly (P less than .05) with increasing BG, although intake was numerically similar with .6, .9 and 1.2% BW of BG. Digestibility was constant with diet (P greater than .10). Six growing Holstein steers used in Exp. 2, a Latin square with a 2 x 3 factorial arrangement of treatments, ingested I or noninfected (NI) fescue hay ad libitum with 0, .5 or 1.0% BW of ground corn. Total DM intake increased linearly as the level of corn rose (P less than .05). Total intake with I increased more with the first than with the second addition of corn, and the opposite occurred with NI (interaction between fescue infection and the quadratic effect of corn level, P less than .10). Organic matter digested (g/d) was greater for NI than for I and rose linearly with increasing corn ingestion (P less than .05). Ninety-six crossbred beef heifers and steers (184.2 kg avg initial live weight) were used in a 77-d fall grazing experiment (Exp. 3) with a 2 x 3 factorial treatment arrangement. Cattle grazed I or NI paddocks and were given no supplement or .34% BW of BG or .65% BW of ground corn on a daily basis (DM).(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗

Feed intake, digestion and digesta characteristics of cattle fed Bermudagrass or orchardgrass alone or with ground barley or corn.

Feed intake, digestion and digesta characteristics of cattle fed bermudagrass (BG) or orchardgrass (OG) alone or with supplemental ground corn or barley were determined in two 6 x 6 latin squares with 2 x 3 factorial treatment arrangements. In Exp. 1, beef cows (Hereford, Angus and Hereford-Angus; 452 kg) cannulated in the rumen and duodenum were fed BG (7.9% CP, 79% NDF and 8.7% ADL) or OG (9.8% CP, 79% NDF and 7.2% ADL) hays at 1.2% of BW per day either alone or with added ground barley (.64% BW) or ground corn (.60% BW daily). The increase in microbial OM flow with corn was greater for OG than for BG; corn elevated microbial OM flow more than did barley with OG but less than with BG (forage type x grain source interaction; P less than .10). The increase in total tract OM digestion with grain was greater for BG than for OG (supplementation effect and forage type x supplementation interaction; P less than 05). In Exp. 2, Holstein steers (228 kg) were fed BG and OG hays ad libitum either alone or with addition of either 1.07% of BW per day of barley or 1.00% BW of corn. Total DM intake was 2.19, 3.03 and 2.82% BW for BG and 2.14, 2.80 and 2.52% BW for OG alone or with barley or corn supplements, respectively, being affected by forage type, grain supplementation, grain type and a forage type x grain supplementation interaction (P less than .05). Organic matter digested daily (g/d) was higher for OG than for BG, higher with than without grain and higher for barley than for corn (P less than .05).

Animal Feed↗

Effects of dry corn gluten feed on feedlot cattle performance and fiber digestibility.

Effects of dry corn gluten feed (DCGF) on feedlot cattle performance and fiber digestibility were investigated. In Trial 1, 120 growing steers were fed corn silage-based diets containing 0, 40, 60 or 80% DCGF. Increasing levels of DCGF resulted in a curvilinear response in gain (P less than .05) and a linear increase in feed/gain (P less than .01). When the same steers subsequently were fed the same levels of DCGF in corn-based diets (Trial 2), increasing the percentage of dietary DCGF resulted in a linear decrease in gain (P less than .01) and a linear increase in feed/gain (P less than .01). In Trial 3, 46 crossbred steers were fed individually in a 2 x 2 factorial design to determine effects of 60 or 80% dietary high-moisture corn (HMC) or DCGF on feedlot cattle performance. Steers fed HMC had faster (P less than .08) and more efficient (P less than .05) gains than those fed DCGF, which had greater feed intakes (P less than .05). In Trial 4, 120 Angus crossbred steers were used to compare effects of 20 or 40% dietary HMC or DCGF on feedlot performance. Steers fed diets containing 40% HMC or DCGF had greater gains (P less than .01) and feed intakes (P less than .01) than those fed 20% diets. Steers fed HMC gained more efficiently than those fed DCGF (P less than .01). In an in situ trial, 0, 40, 60 or 80% dietary DCGF did not affect in situ DCGF DM or NDF disappearance. When DCGF was fermented in vitro in combination with corn silage, increasing the level of DCGF from 0 to 100% resulted in a linear increase (P less than .01) in 24 and 48 h NDF disappearance. These results suggest that at high dietary levels DCGF will support feedlot cattle gains that are nearly equal to those of cattle fed corn silage but somewhat less than those fed corn.

Animal Feed↗

Effects of brown midrib 3 mutation in corn silage on productivity of dairy cows fed two concentrations of dietary neutral detergent fiber: 1. Feeding behavior and nutrient utilization.

The effects of digestibility of corn silage neutral detergent fiber (NDF) and dietary NDF content on feeding behavior, dry matter intake (DMI), and energy utilization were evaluated with eight multiparous high producing dairy cows in a duplicated 4 x 4 Latin square design with 21-d periods. Experimental diets contained corn silage from a brown midrib (bm3) hybrid or its isogenic normal control at two concentrations of dietary NDF (29 and 38%). Both low NDF diets and bm3 corn silage treatments increased DMI and milk yield. However, an interaction between corn silage treatment and dietary NDF content was observed for meal size and for interval between meals, implying that different mechanisms regulating DMI dominate depending on the fermentation characteristics of diets. The bm3 treatment depressed milk fat concentration when fed in a low NDF diet. The bm3 corn silage increased solids-corrected milk yield, but did not affect daily body weight gain compared with control, whereas low NDF diets increased daily body weight gain, but did not affect solids-corrected milk yield compared to high NDF diets. Both bm3 treatments and low NDF diets reduced ruminal pH, but low NDF treatments increased fluctuation of ruminal pH and bm3 treatments did not. Feeding bm3 corn silage increased the energy utilized for milk production, possibly because of a consistent supply of metabolic fuels from the rumen. The beneficial effects of bm3 corn silage on productivity of lactating cows were greater for the cows fed a high NDF diet.

Animals↗

Effects of brown midrib corn silage on the energy balance of dairy cattle.

Effects of genotype and level of intake on net energy for lactation values of corn silage were evaluated by indirect calorimetry in two experiments using lactating and dry, nonpregnant dairy cows. In experiment 1, six multiparous Holstein cows in early lactation were fed experimental diets containing either brown midrib (bm3) or isogenic normal corn silage. Dietary treatments were isogenic and bm3 diets fed ad libitum, and the bm3 diets restricted-fed. Dry matter (DM) intake was 2.4 kg/d greater for cows fed the bm3 diet ad libitum compared with cows fed the isogenic diet. Apparent digestibilities of DM, organic matter, neutral detergent fiber, and acid detergent fiber were greater for cows restricted-fed bm3 than the isogenic diet. In experiment 2, six dry, nonpregnant Holstein cows were fed maintenance diets containing either bm3 or isogenic corn silage. Apparent digestibilities of DM, organic matter, neutral detergent fiber, and acid detergent fiber were greater for cows fed bm3 compared with isogenic corn silage. Digestible energy and metabolizable energy were greater for maintenance diets containing bm3 compared with isogenic corn silage, respectively. These data indicate increased milk production seen in other studies is a result of increased DMI rather than an increase in energy efficiency. Increased organic matter digestibility of bm3 corn silage resulted in greater digestible energy and metabolizable energy values in cows fed at maintenance energy intake. However, calculated net energy for lactation values of bm3 and isogenic corn silages were similar at both productive and maintenance levels of feeding.

Animals↗

Cracked dry or finely ground high moisture shelled corn as a supplement for grazing cows.

Feeding shelled corn as the primary supplement in cracked dry form or ground high moisture form to grazing cows was studied in an experiment using 32 cows in late lactation. Each of the two forms of corn accounted for 74.7% of the concentrate supplement, which was fed at 9 kg/d (DM). The experiment was 13 wk, during which cows grazed permanent native pasture containing mostly grasses and some clover. Milk yield was 2.4 kg/d higher for cows fed ground high moisture shelled corn than for those fed dry cracked shelled corn. Milk fat content was lower (3.28 vs. 3.67%), but protein content was higher (3.26 vs. 3.15%) for the high moisture corn supplement. Results suggest that high moisture corn can serve as a better supplement for grazing cows than dry corn, likely by providing more metabolizable energy and microbial protein due to more fermentable and digestible starch.

Animal Feed↗

Rumen acid production from dairy feeds. 1. Effects on feed intake and milk production of dairy cows offered grass or corn silages.

Earlier studies developed a new approach to feed evaluation, measuring the net acid load that develops during rumen fermentation. Two concentrates were formulated to be isoenergetic and isonitrogenous, with extremes of rumen acid load. A third treatment comprised a 50:50 mixture of these concentrates. These concentrates were evaluated along with ryegrass silage and corn silage. The feeds were evaluated in a continuous culture system adapted to deliver and record the quantities of acid or alkali needed to maintain a constant pH (6.2 to 6.3). This study confirmed the anticipated ranking of concentrates for rumen acid load, as well as the highly acidogenic nature of corn silage. The concentrates were formulated to balance corn silage and were offered to early-lactation Holstein-Friesian cows at 50% of dry matter intake, with either ryegrass silage or corn silage. Feed intake was lower for animals offered corn silage-based diets (17.4 vs. 22.2 kg of dry matter/d). Increasing concentrate acid load led to a large decline in dry matter intake for corn silage, although not for grass silage. Feed intake effects were reflected in significant effects on yield of milk (31.0, 29.9, and 26.9 kg/d for low-, medium-, and high-acid load concentrates, respectively) and milk solids. Milk protein concentration was unaffected by concentrate type with corn silage diets but tended to be higher when high acid load concentrates were fed with grass silage. This may reflect the effect of the high starch concentrate rectifying a shortage of glucogenic precursors or microbial protein with the grass silage-based diet.

Animal Feed↗

Performance of lactating dairy cows fed corn as whole plant silage and grain produced from a glyphosate-tolerant hybrid (event NK603).

Sixteen multiparous Holstein cows averaging 74 d in milk were used in a replicated 4 x 4 Latin square to compare the effects on animal performance of feeding whole plant silage and grain from a glyphosate-tolerant corn hybrid (event NK603), a nontransgenic control hybrid, and two commercial nontransgenic hybrids (DK647 and RX740). The grain and silage from the four corn hybrids were produced using the same procedures and under similar agronomic conditions at the University of Illinois. On a dry matter (DM) basis, diets contained 30% corn silage and 27.34% corn grain produced either from event NK603, a nontransgenic control, or commercial hybrids. Apart from the DM content of silages, the chemical composition of both grain and silage produced from the four corn hybrids were substantially equivalent. Feeding diets that contained event NK603 and DK647 hybrids tended to decrease DM intake (DMI) compared with the control nontransgenic and RX740. The intakes of crude protein (CP), acid and neutral detergent fiber, and nonfiber carbohydrates were not different for cows fed event NK603 and control diets. The RX740 diet resulted in the highest intakes of fiber and CP, whereas the DK647 diet resulted in the lowest intake of CP. These differences in nutrient intake arose from small variations in both the DMI and the chemical composition of feed ingredients and experimental diets. Production of milk and 3.5% fat-corrected milk; milk fat, CP, and true protein percentage and yield; milk urea N; milk total solids percentage and yield; and somatic cell count were not affected by treatments. These data indicate that the stable insertion of the gene that confers tolerance to glyphosate (event NK603) in the corn line used in this experiment does not affect its chemical composition and nutritional value for lactating dairy cows when compared with conventional corn.

Animals↗

Effects of cutting height and maturity on the nutritive value of corn silage for lactating cows.

We studied the effect of increasing the cutting height of whole-plant corn at the time of harvest from 12.7 (NC) to 45.7 (HC) cm on yield and nutritive value of silage for dairy cows. Three leafy corn silage hybrids were harvested at NC and HC at about 34% dry matter (E) and 41% DM (L) and ensiled in laboratory silos. Increasing the height of cutting lowered yields of harvested DM/ha. In addition, the concentrations of DM and starch were higher but the concentrations of lactic acid, crude protein, neutral detergent fiber (NDF), and acid detergent fiber were lower in HC than in NC. The concentration of acid detergent lignin was also lower in HC, but only in corn harvested at E. In vitro digestion (30 h) of NDF was greater in HC (50.7%) than NC (48.3%). Calculated yield of milk per tonne of forage DM was greater for HC than for NC at E but not at L. In a lactation experiment, increasing the height of cutting of another leafy corn silage hybrid, TMF29400, in general also resulted in similar changes in nutrient composition as just described. When fed to lactating dairy cows, HC corn silage resulted in tendencies for greater NDF digestion in the total tract, higher milk production and improved feed efficiency, but there were no differences in 3.5% fat corrected milk between treatments. Results of this study suggest that increasing the cutting height of whole plant corn at harvest can improve the nutritive value of corn silage for lactating dairy cows.

Animal Nutritional Physiological Phenomena↗

Fermentation characteristics of corn forage ensiled in mini-silos.

To evaluate numerous experimental variables and their interactions involving different corn (Zea mays, L.) silage hybrids, scaled down mini-silos are necessary. Objectives of this study were to evaluate the influence of sample size on pH, NH3, and volatile fatty acid profile of 8 corn silage hybrids, selected to vary in fiber digestibility and ensiled in vacuum-sealed polyethylene bags for 90 d, and to assess the suitability of these mini-silos for detecting differences among corn silage samples. Hybrids were grown at the Cornell Teaching and Research Center located near Harford, NY, and harvested at a dry matter content of about 32% in the fall of 2002. Samples from 3 field replications of each hybrid were chipper-shredder chopped and vacuum-ensiled in bags with sample sizes of 50, 100, 200, 400, and 600 g. Increasing sample size resulted in decreased lactic acid, acetic acid, total acids, and NH3. Most of the difference among sample sizes occurred between the 50- and 100-g sample sizes. Lactic acid:acetic acid ratio (3.1 +/- 0.13) and pH (3.9 +/-0.08) did not vary among sample sizes. There was no detectable butyric acid in the samples. Fermentation characteristics suggested that all samples were well ensiled but that the fermentation profile of the 50-g samples differed the most from other sample sizes. Hybrids did vary in lactic acid, acetic acid, lactic acid:acetic acid, and pH. Differences among hybrids were also noted for dry matter and crude protein. Field-chopped corn hybrids that were ensiled using mini-silos had higher acids than corresponding field-chopped corn hybrids ensiled in Ag-bags, in part due to no effluent escaping from the mini-silos. It is possible to use vacuum-sealed plastic bags to ensile corn, with samples as small as 200 g, and to use these mini-silos to assess differences among corn silage samples. Caution should be used when extrapolating mini-silo data to field-scale ensiling.

Ammonia↗

Effects of corn silage particle length and forage:concentrate ratio on milk fatty acid composition in dairy cows fed supplemental flaxseed.

This study aimed to evaluate the effects of length of chop of corn silage and forage:concentrate ratio (F:C) on performance and milk fatty acid profiles in dairy cows supplemented with flaxseed. Our hypothesis was that decreasing forage particle length and F:C ratio would increase unsaturated fatty acid flow to the small intestine and subsequent transfer of these unsaturated fatty acids into milk. Eight Holstein cows (648.1 +/- 71.5 kg body weight; 109.6 +/-43.6 days in milk) were used in a replicated 4 x4 Latin square design with 21-d periods and a 2 x2 factorial arrangement of dietary treatments. Dietary factors were: 1) F:C ratios (dry matter basis) of 55:45 and 45:55; and 2) corn silage particle lengths of 9.52 and 19.05 mm. All experimental cows received 1 kg of flaxseed to substitute for 1 kg of a rolled barley grain-based concentrate daily. Diets were fed twice daily as a total mixed ration. Corn silage particle length and F:C ratio had no effect on dry matter intake, milk yield, and milk composition; however, feeding short cut corn silage depressed milk protein yield. Significant particle size xF:C ratio interactions were observed for milk fat proportions of C(16:0), C(18:1) cis-9, and C(18:2) cis-9, trans-11 (a conjugated linoleic acid isomer). At short corn silage particle size, decreasing F:C ratio depressed milk fat proportion of C(16:0). Conversely, feeding short corn silage at high F:C ratio increased the proportion of C(18:1) cis-9 and C(18:2) cis-9, trans-11 in milk fat. The milk fat proportion of C(18:2) trans-10, cis-12, a conjugated linoleic acid isomer that is associated with milk fat depression, was not affected by dietary treatment. Our results show that corn silage particle length and F:C ratio influence milk fatty acid profiles in dairy cows fed supplemental flaxseed as a source of polyunsaturated fatty acids.

Animal Nutritional Physiological Phenomena↗

Rumen volatile fatty acids and milk composition from cows fed hay, haylage, or urea-treated corn silage.

Alfalfa-brome hay, haylage, .5% urea-treated corn silage, or .5% urea plus 1% dried whey-treated corn silage was fed as the only forage to one of four groups of 10 lactating cows per group for a lactation trial of 10 wk. Rumen samples were collected via stomach tube 3 to 4 h after the morning feeding. The pH of the rumen samples from cows fed hay was higher than for cows fed haylage, urea-treated corn silage, and urea-whey corn silage, 6.69 versus 6.36, 6.40, and 6.50. Total volatile fatty acids and propionate were highest from cows fed urea-whey corn silage and were higher on all three fermented forages than cows fed hay. Acetate/propionate ratio was highest from cows fed hay and lowest from cows fed corn silages. Butyrate was highest from cows fed haylage or hay. Milk protein composition was not affected by ration although nonprotein nitrogen of milk was highest from cows fed the urea-treated corn silages. Oleic acid and total unsaturated fatty acids were lowest in milk fat from cows fed hay while palmitic acid was highest from cows fed hay and haylage. These results suggest that type of forage fed may cause small changes in rumen fermentation and in milk composition. The importance of these changes is unknown but may affect properties of dairy products produced from this milk.

Animal Feed↗