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A comparison of two methods of adhering composite to metal.

Composites are used as an esthetic restorative material in prosthetic dentistry, often with a metallic substructure that provides support when large restorations are required. Different bonding systems have been proposed to occupy the gap between the composite and metal and to reduce marginal leakage at this interface, which with time results in a degradation of the metal-composite junction with dissociation of these two materials. This study compared two methods of adhesion of composite to metal: (1) the Silicoater, based on a silanization of metal and (2) the Spectralink, based on an ionization of metal. A total of 108 metal pellets were covered with composite; 36 pellets formed the baseline or control group and 72 pellets were placed on temporary fixed partial dentures for the 12 patients in the study. The two bonding methods were compared at 0, 30, and 90 days. Marginal leakage (< 0.7 microns) and marginal gap (< 0.6 microns) for the baseline groups were minimal and demonstrated no significant difference between the two samples. The degradation of marginal adaptation and hermeticity was faster and more severe with the pellets of the ionization sample. The Silicoater samples exhibited a better hydrolytic resistance with the formation of a hydrophobic polymer. The degradation of the composite-metal interface with the Spectralink method appeared to be the result of the hydrophilic character of polyfluoromethacrylate. Nevertheless, the values of the marginal gap (< 6.0 microns) and the microleakage (< 30.0 microns) remained low for the metal treatments.

Acrylic Resins↗

Relationship between dimensions of adaptive behavior and sheltered workshop productivity.

A factor analysis was performed on the 24 domains of the AAMD Adaptive Behavior Scale using a sample of 217 adult retarded workshop clients. Of the seven dimensions found to describe adaptive behavior, Personal Independence, Social Maladaptation, and Personal Maladaptation accounted for the majority of variance in the factor matrix. Scores on each of the seven factors were obtained for all clients and used to predict productivity in a workshop setting as defined by average hourly salary for the time period in which the scale was administered. A stepwise regression analysis yielded a highly significant regression effect in which the factors of Personal Independence and Social Maladaptation accounted for 25% of the variance in salary and had a multiple correlation coefficient of .50 with the predicted variable. We concluded that adaptive behavior is a multidimensional variable, significantly affecting productivity in sheltered workshop settings.

Adaptation, Psychological↗

A colorimetric assay for the simultaneous measurement of plasminogen activators and plasminogen activator inhibitors in serum-free conditioned media from cultured cells.

The coupled photometric assay for plasminogen activator reported by Coleman and Green (1981) Methods in Enzymology (Lorand, L., Ed.), Vol. 80, pp. 408-414, Academic Press, San Diego, CA) has been adapted for use with 96-well plates and an automatic microplates spectrophotometer. The assay allows the discrimination between tissue-type and urokinase-type plasminogen activators in cell culture-conditioned media. It provides a level of detection of these enzymes in the range 10(-17) to 10(-13) mol (determined using purified human plasminogen activators), uses no radioisotopes, and is faster and more economical than similar assays using specific peptide substrates for plasminogen activators. Levels of free plasminogen activator inhibitor activity can be simultaneously measured on the same samples by a simple adaptation of the assay. This method allows an easy treatment of the data by interfacing with a computer and should thus be useful when large numbers of samples are assayed.

Adrenal Gland Neoplasms↗

Classification of patients with whiplash associated disorders (WAD): reliable and valid subgroups based on the Multidimensional Pain Inventory (MPI-S).

BACKGROUND: Classification of patients with chronic whiplash associated disorders (WAD) into homogenous subgroups is an important objective in order to tailor interventions and to control for subgroup differences when evaluating treatment outcome. AIMS: The aims of this study were to investigate if it was possible to replicate and describe the three cluster solution and profiles found in other pain groups and describe cluster profiles based on self-reported Multidimensional Pain Inventory-scores for patients with WAD three months after the injury, describe characteristics of the clusters in relation to disability, self-efficacy and coping at the same point in time and to validate the cluster solution by comparing clusters in disability, self-efficacy and coping over time. METHODS: Ninety-one WAD-patients three months after the accident took part in the study. The measures used were the Multidimensional Pain Inventory-Swedish version (MPI-S), The Self-Efficacy Scale, The Coping Strategies Questionnaire and The Pain Disability Index. Cluster analysis was conducted for the total sample MPI-S subscale scores. RESULTS: The adaptive copers cluster represented 42% of the sample, dysfunctional 34% of the sample, and interpersonally distressed 24% of the sample. The external validation of cluster solution showed that there were several significant differences between clusters in self-efficacy, disability and coping measures. There was also a significant interaction effect (clusterxtime) in disability (PDI). Patients in dysfunctional cluster reported a decreased disability over time. CONCLUSIONS: These results support the presence of different subgroups among patients with whiplash associated disorders. This classification can be seen as a complement to a classification based on medical condition.

Adaptation, Psychological↗

[First grade students in former East and West Berlin: socio-emotional adjustment and relation to attachment patterns of the children].

Adaptation to school of two samples of children (45 from East and 43 from West Berlin) grown up in two different political systems until the "Mauerfall" was studied over the first two school years using information from teachers in school and kindergarten, parents, and the children themselves. Cluster analysis revealed 3 patterns of adaptation behavior: the first subgroup (61% of the sample) showed no problems at all, for a second subgroup (22%) teachers reported more adaptation difficulties, whereas the third subgroup (17%) was rated by their mothers as distractible, hard to motivate and less sociable. Sex differences turned out to be more significant than political-system differences. Adjustment patterns to school were also related to the child's quality of attachment, to the reported number of stressful life events happened to the family, as well as to mother's and teacher's existential anxiety.

Affective Symptoms↗

Parameter estimation of an asymmetric vocal-fold system from glottal area time series using chaos synchronization.

In this paper, we apply an iterative parameter adaption scheme based on chaos synchronization to estimate system parameters of the asymmetric vocal folds from glottal area time series. The original asymmetric vocal-fold system associated with recurrent laryngeal paralysis shows chaotic vibrations with positive Lyapunov exponents. Aperiodic glottal area time series from the original system will be applied as the feedback variable coupling the simulative and the original vocal-fold systems. The parameter adaption technique based on chaos synchronization is employed to manipulate the simulative system parameters. The chaotic vibrations, system parameters, and the bifurcation diagram of the original vocal-fold system can be exactly reproduced in the simulative system, and the two chaotic systems can be synchronized. Furthermore, the effects of noise, sampling rate, and equation difference due to nonlinear spring terms on vocal-fold parameter estimations are investigated. Despite large noise perturbations, large equation differences, and low sampling rate, the parameter adaption scheme can effectively estimate the original vocal-fold system parameters. This study provides a theoretical base to apply chaos synchronization to estimate the vocal-fold system parameters from the glottal area data and show its potential application in laryngeal physiology.

Algorithms↗

Computer-controlled mechanical testing machine for small samples of biological viscoelastic materials.

Time dependency in the mechanical properties of viscoelastic materials means that a variety of tests is often required to fully characterize their properties. Computer control is the best way of controlling loads and displacements and the rate at which they are applied, as well as recording and analysing the data produced. This paper describes apparatus for measuring the viscoelastic properties of articular cartilage in compression which is readily adaptable to any small sample in which accurate strain measurements require small, carefully controlled displacements. An IBM-type microcomputer is used to control a stepper motor driving a ball screw with a positional accuracy of about 1 micron.

Biomechanical Phenomena↗

Survey of equine rotaviruses shows conservation of one P genotype in background of two G genotypes.

DIG-labelled ssRNA probes were prepared from variable regions of VP4 and VP7 cognate genes, and used in hybridization assays for P and G genotyping of group A cell culture-adapted equine rotaviruses and fecal samples collected from foals with and without diarrhea. The probes confirmed known P and G serotypes of sixteen cell culture-adapted strains. From one-hundred and twenty-one rotavirus-positive samples, 83 reacted when tested for their P and G genotype specific probes. From these, 71 were found to contain G3 P12 genotypes, and 11 G14 P12 genotypes. No sample reacted with H1 or L338 P and G genotype probes. This suggests that the equine rotavirus population is conservative, containing predominantly one P genotype and two G genotypes. One isolate (26/94) whose dsRNA was visualized in an agarose gel did not react with any of the equine probes, and was found to belong to G8 and P1 genotypes. This is the fourth example of a single unique equine isolate (after H1, L338, and R-22). The remaining thirty-eight untypable field isolates had no detectable dsRNA after storage for 1 to 3 years.

Animals↗

A two-stage cyclone using microcentrifuge tubes for personal bioaerosol sampling.

Personal aerosol samplers are widely used to monitor human exposure to airborne materials. For bioaerosols, interest is growing in analyzing samples using molecular and immunological techniques. This paper presents a personal sampler that uses a two-stage cyclone to collect bioaerosols into disposable 1.5 ml Eppendorf-type microcentrifuge tubes. Samples can be processed in the tubes for polymerase chain reaction (PCR) or immunoassays, and the use of multiple stages fractionates aerosol particles by aerodynamic diameter. The sampler was tested using fluorescent microspheres and aerosolized fungal spores. The sampler had first and second stage cut-off diameters of 2.6 microm and 1.6 microm at 2 l min(-1)(geometric standard deviation, GSD = 1.45 and 1.75), and 1.8 microm and 1 microm at 3.5 l min(-1)(GSD = 1.42 and 1.55). The sampler aspiration efficiency was >or=98% at both flow rates for particles with aerodynamic diameters of 3.1 microm or less. For 6.2 microm particles, the aspiration efficiency was 89% at 2 l min(-1) and 96% at 3.5 l min(-1). At 3.5 l min(-1), the sampler collected 92% of aerosolized Aspergillus versicolor and Penicillium chrysogenum spores inside the two microcentrifuge tubes, with less than 0.4% of the spores collecting on the back-up filter. The design and techniques given here are suitable for personal bioaerosol sampling, and could also be adapted to design larger aerosol samplers for longer-term atmospheric and indoor air quality sampling.

Aerosols↗

Optimization of square wave anodic stripping voltammetry (SWASV) for the simultaneous determination of Cd, Pb, and Cu in seawater and comparison with differential pulse anodic stripping voltammetry (DPASV).

Square wave anodic stripping voltammetry (SWASV) was optimized for the simultaneous determination of Cd, Pb and Cu in coastal seawater samples. Background subtraction was adapted to improve peak detection and quantification. Optimum background voltammograms were obtained by applying a 7.5 s equilibration potential at -975 mV (vs. Ag/AgCl, 3M KCl) before starting the background scan. Voltammetric scan parameters were optimized to obtain maximum sensitivity while retaining good peak resolution and discrimination from background. Optimal parameters were: frequency 100 Hz, pulse amplitude 25 mV, current sampling delay time 2 ms, step height 8 mV. The sensitivity of optimized SWASV proved to be more than double that of differential pulse anodic stripping voltammetry (DPASV), and analysis time was halved. Samples containing around 13 (Cd), 30 (Pb), 200 (Cu) ng/l (typical averages of the coastal area of the Marche region) can be analyzed using a 5 min deposition time and the total analysis time using three standard additions is about 1 h and half, excluding the mercury film preparation and the outgassing of the sample, which can be made in parallel using a second cell cup.

Cadmium↗

The prevalence of rheumatic diseases in a Filipino urban population: a WHO-ILAR COPCORD Study. World Health Organization. International League of Associations for Rheumatology. Community Oriented Programme for the Control of the Rheumatic Diseases.

OBJECTIVE: To determine the point prevalence of musculoskeletal complaints and rheumatic diseases in a Filipino urban community. METHODS: A descriptive cross-sectional 2 phase survey was conducted in an urban community in Metropolitan manila. Phase I (screening) used face-to-face interviews, while phase II (examination) involved case identification of the rheumatic diseases. We sampled 670 households (3065 adults) using a multistage cluster sampling method. A pilot study was conducted to pretest the questionnaire for cross-cultural adaptation and validation, field procedures, sampling design, and data management plan. Standardized translated COPCORD questionnaires (blind translation and blind back-translation) were administered by trained interviewers. Two weeks after Phase I, Phase II was conducted at local health centers. The COPCORD questionnaire screened the number of cases with musculoskeletal complaints. Identification of cases with rheumatic disease was based on American College of Rheumatology (ACR) criteria. RESULTS: Respondents completed 3006 questionnaires (phase I response rate 98%). Of these 489 respondents had musculoskeletal complaints. Functional disability was reported in 25% among these respondents. We examined 353 (phase II response rate 72%), revealing 294 with rheumatic conditions. In 26 persons there were no abnormalities, while 32 had nonrheumatic conditions at examination. The most common rheumatic diseases were osteoarthritis (OA) (n = 124) and soft tissue rheumatism (n = 115). CONCLUSION: The prevalence of musculoskeletal complaints was 16.3% (95% CI 8.6-24.0) of the adult population in a FIlipino urban community. The total prevalence of rheumatic disease is 9.8% (95% CI 8.2-11.4). The prevalence of OA was 4.1% (95% CI 3.3-4.9) and soft tissue rheumatism 3.8% (95% CI 2.9-4.8). The prevalence of rheumatoid arthritis, 0.17% (95% CI 0-9.36), was notably low compared to the prevalence in other developing countries.

Adolescent↗

Evolutionary genomics of Culex pipiens: global and local adaptations associated with climate, life-history traits and anthropogenic factors.

We present the first genome-wide study of recent evolution in Culex pipiens species complex focusing on the genomic extent, functional targets and likely causes of global and local adaptations. We resequenced pooled samples of six populations of C. pipiens and two populations of the outgroup Culex torrentium. We used principal component analysis to systematically study differential natural selection across populations and developed a phylogenetic scanning method to analyse admixture without haplotype data. We found evidence for the prominent role of geographical distribution in shaping population structure and specifying patterns of genomic selection. Multiple adaptive events, involving genes implicated with autogeny, diapause and insecticide resistance were limited to specific populations. We estimate that about 5-20% of the genes (including several histone genes) and almost half of the annotated pathways were undergoing selective sweeps in each population. The high occurrence of sweeps in non-genic regions and in chromatin remodelling genes indicated the adaptive importance of gene expression changes. We hypothesize that global adaptive processes in the C. pipiens complex are potentially associated with South to North range expansion, requiring adjustments in chromatin conformation. Strong local signature of adaptation and emergence of hybrid bridge vectors necessitate genomic assessment of populations before specifying control agents.

Adaptation, Biological↗

Molecular monitoring of the intestinal flora by denaturing high performance liquid chromatography.

Gut flora analysis is hampered by the complexity of the intestinal microbiota and by inherent limitations of culture-based approaches. Therefore, culture-independent molecular methods based upon 16S rRNA gene analysis were applied successfully for the analysis of complex microbial communities. However, generally accepted and validated profiling methods such as denaturing and temperature gradient gel electrophoresis (DGGE/TGGE) are still laborious and time consuming. Thus, we adapted the separation of amplified bacterial 16S rRNA gene fragments by denaturing high performance liquid chromatography (DHPLC) using the WAVE Microbial Analysis System as a rapid and convenient means to display complex intestinal bacterial communities and to monitor changes in the gut flora. The separation of 16S rRNA gene fragments amplified from reference strains representing main gut bacterial populations and from human stool samples revealed that DHPLC analysis effectively detects bacterial groups predominant in the human gut flora. The investigation of faecal samples from hospitalized patients before, during and after antibiotic therapy showed that PCR-based DHPLC can be used to monitor gut flora changes. Results from DHPLC analysis were comparable with DGGE profiles generated from the same samples, demonstrating that the adapted DHPLC protocol is well suited for the analysis of complex microbial communities.

Chromatography, High Pressure Liquid↗

Efficacy of filter types for detecting Campylobacter jejuni and Campylobacter coli in environmental water samples by polymerase chain reaction.

A previously developed polymerase chain reaction (PCR) amplification of a target region in the flaA Campylobacter flagellin gene was evaluated and adapted for use with environmental water samples. The ability to detect Campylobacter jejuni or Campylobacter coli in seeded water samples was tested with various filters after concentration and freeze-thaw lysis of the bacterial cells. A nonradioactive probe for the amplified flagellin gene fragment detected as little as 1 to 10 fg of genomic DNA and as few as 10 to 100 viable C. jejuni cells per 100 ml of water filtered onto Fluoropore (Millipore Corp.) filters. No amplification was obtained with cellulose acetate filters, most likely because of binding of the DNA to the filter. Concentration and lysis of target cells on Fluoropore and Durapore (Millipore Corp.) filters allowed PCR to be performed in the same reaction tube without removing the filters. This methodology was then adapted for use with environmental water samples. The water supply to a broiler chicken production farm was suspected as the source of C. jejuni known to be endemic in grow-out flocks at the farm, despite the inability to culture the organisms by standard methods. The filtration-PCR method detected Campylobacter DNA in more than half of the farm water samples examined. Amplified campylobacter DNA was not detected in small volumes of regional surface water samples collected on a single occasion in February. The filtration-PCR amplification method provided a basis for detection of C. jejuni and C. coli in environmental waters with a high degree of specificity and sensitivity.

Base Sequence↗

PCDD/PCDF congener profiles in soil and herbage samples collected in the vicinity of a municipal waste incinerator before and after pronounced reductions of PCDD/PCDF emissions from the facility.

Congener profiles are the fractional distribution of polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) congeners in an environmental release, or in an environmental or biological sample. In 1999, an adaptation to the EU legislation on pollutant emissions from the stack was carried out in an old municipal solid waste incinerator (MSWI) from Montcada (Barcelona, Spain). The main goal of the present study was to determine if the environmental PCDD/F levels in the area under direct influence of the facility were mainly due to PCDD/F emissions from the plant. For this purpose, soil and herbage samples were collected near the MSWI before (1998) and after (2000) the technical improvements were performed. PCDD/F congener profiles were determined and compared with those from samples collected in a suburban area of Constantí (Tarragona, Spain) outside of direct emissions from any MSWI. The results of the present study suggest that the MSWI here assessed is not the main responsible for the environmental PCDD/F concentrations in the area under evaluation. Other PCDD/F emission sources in the same area seem also to have a notable impact on the atmospheric levels of these pollutants.

Air Pollutants↗

Method for the simultaneous establishment of many axenic cultures of Paramecium.

A method is described for the simultaneous treatment of 42 (or more) stocks of Paramecium, and their adaptation to growth in axenic culture. Samples of dense cultures of these ciliates growing with Enterobacter aerogenes are rendered bacteria-free by migration through 2 sets of tubes containing Adaptation Medium (Peters' salts solution, stigmaterol, vitamins, and autoclaved E. aerogenes). The 2nd set of tubes contains Adaptation Medium plus antibiotics. Bacteria-free samples containing approximately 100 animals are then transferred to test tubes containing Adaptation Medium without antibiotics. This medium also serves as a growth medium. It supports indefinite growth of all Paramecium stocks tested. After adaptation to this medium, the ciliattes can be grown in the axenic medium developed by Soldo, Godoy & van Wagtendonk. On a single trial at least half of the stocks can be expected to produce axenic cultures within 5 to 10 days by these procedures. The method has been applied successfully to several of the species of the Paramecium aurelia complex, to all syngens of Paramecium multimicronucleatum, to several stocks of Paramecium jenningsi, and to 1 stock of Paramecium caudatum and Paramecium calkinsi. A modification of the method also works for Didinium nasutum.

Animals↗

On-line antioxidant activity determination: comparison of hydrophilic and lipophilic antioxidant activity using the ABTS*+ assay.

The ABTS/H(2)O(2)/HRP decoloration method is capable of determining both hydrophilic (in buffered media) and lipophilic (in organic media) antioxidant properties in complex samples. Now, we have adapted this method for on-line chromatographic determination. The easy, rapid and controlled generation of the ABTS radical and its great stability in buffered and organic media were important characteristics in the measurement of antioxidant activities. The HPLC-ABTS method used two pumps (one for isocratic eluting-phase and the other for preformed ABTS radical) and an UV-VIS diode array detector. The dual analysis of samples -- conventional (with UV-VIS detection) and ABTS-scavenging (at 600 nm) -- provided valuable on-line information about the correspondence between the presence of a determined compound and its possible antioxidant activity, and was applicable to both hydrophilic and lipophilic antioxidants (HAA and LAA). A comparison between HAA and LAA determined by the end-point method and by the on-line HPLC method is presented. The application to juices showed that both methods are suitable, sensitive and selective, gave similar values, and the HPLC-ABTS method contributed additional information about the antioxidant activity profile.

Antioxidants↗

A 12-month controlled trial of methadone medical maintenance integrated into an adaptive treatment model.

Methadone medical maintenance (MMM) reduces the reporting schedule for stable and well-functioning methadone maintenance patients to once a month, with counseling provided by medical staff. We report on the 12-month outcomes of 92 highly stable methadone maintenance patients randomly assigned to one of three study conditions: routine care, MMM at the methadone maintenance program, and MMM at a physician's office. Methadone medical maintenance patients received a 28-day supply of methadone, whereas routine care patients received five or six take-home methadone doses each week. All patients performed a medication recall once a month and submitted two urine samples each month. An adaptive stepped-care system of treatment intensification was used for patients who failed recall or who had drug-positive urine specimens. Seventy-seven patients completed the 12-month study period. Dropout was caused primarily by problems with handling methadone and disliking the recall frequency. There were low rates of drug use or failed medication recall. Treatment satisfaction was high in all groups, but the MMM patients initiated more new employment or family/social activities than did routine care patients over the study period. The stepped-care approach was well tolerated and matched patients to an appropriate step of service within a continuum of treatment intensity.

Adult↗