[Metabolism of C14-labelled urea in conventional, germ-free and specifically associated rats].
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Eight cases of cerebral abscesses due to Proteus mirabilis in the newborn period are reported. The authors emphasize the insidiousness of the clinical features, and the use of ultrasound screening for an early recognition, essential for the therapeutic results.
A newborn infant with Proteus mirabilis meningitis developed multiple brain abscesses with diameters ranging from 2 to 4 cm. Intravenous antibiotic therapy alone without surgical intervention led to the complete resolution of this complication. The case supports that this may be an acceptable treatment of multiple brain abscesses in neonates. However, the clinical outcome was relatively poor, since a porencephalic cyst, hypodense areas in CT scan, and psychomotor retardation were found at one year of age.
A total of 80 patients with postnatal purulent mastitis were treated with gentamicins. The main causative agent was Staph. aureus resistant to the traditionally used antibiotic and sensitive to gentamicin, fusidin, rifampicin and semisynthetic penicillinase-stable penicillins. Gramnegative bacteria contaminated the purulent foci after opening. Gentamicin was highly effective in treatment of postnatal purulent mastitis in cases with mixed Staphylococcus-Proteus infection.
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The work presents the comparative evaluation of different methods used for differentiating bacteria of the genus Proteus. A scheme of the combined typing of Proteus is presented. This scheme includes the determination of enzymatic and serological variants with the subsequent additional bacteriocinogenic and phage typing. Among the Proteus strains in our collection (600 strains), 123 sero-enzymatic variants, 71 enzymo-lysogenic variants, 64 enzymo-bacteriocinogenic variants, 162 sero-lysogenic variants, 164 sero-bacteriocinogenic variants and 52 bacteriocinogeno-lysogenic variants have been detected.
During 1985 four external quality assessment tests for clinical microbiology were performed, each consisting of four simulated clinical specimens. The results are reported, evaluated and some problem areas discussed.
More than 7,000 bacteriological samples were received by the authors and were analysed for the frequency of resistance among pyogenic cocci which are relevant to surgical practice. Staphylococcus aureus accounted for 41 per cent and Escherichia coli for 15.3 per cent. These were the most common germs in 1984. Resistance of Staphylococcus aureus to ampicillin was as high as 78 per cent. Resistance levels below eight per cent were recorded in the context of lincomycin, erythromycin, gentamicin, oxacillin, and berlocombin. Resistance ratios of Escherichia coli were 55.2 per cent to ampicillin, 4.5 per cent to gentamicin, and 12.6 per cent to berlocombin.
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The author studied the dynamics of toxin formation of the high- and low-virulent Pr. mirabilis strains. The high-virulent strain produced toxic substances of the exotoxin type detectable in 1-2-day broth cultures. Later the activity of the culture medium of both cultures under study was due to substances of the endotoxin type. By physico-chemical and immunobiological properties, and also by chemical composition of Pr. mirabilis "early toxin" was similar to the exoenterotoxins of enterobacteria. A principal possibility of concentration and purification of Pr. mirabilis "early toxin" with the use of ultrafiltration and gel-chromatography was demonstrated.