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Building permanent memory codes: codification and repetition effects in word identification.

The studies presented in this article investigate the memory processes that underlie two phenomena in threshold identification: word superiority over pseudowords and the repetition effect (a prior presentation of an item facilitates later identification of that item). Codification (i.e., the development of a single memory code that can be triggered even by fragmented input information) explains the faster and more accurate identification of words than pseudowords. Our studies trace the development and retention of such codes for repeated pseudowords and examine the growth and loss of the repetition effect for both pseudowords and words. After approximately five prior occurrences, words and pseudowords are identified equally accurately in two types of threshold identification tasks, suggesting codification has been completed for pseudowords. Although the initial word advantage disappears, the accuracy of identification still increases with repetitions. The facilitation caused by repetition is not affected much by spacing within a session, but drops from one day to the next, and after a delay of one year has disappeared (new and old words were identified equally well). These results suggest an episodic basis for the repetition effect. Most important, after one year, performance is equal for old pseudowords and new and old words: all these levels are superior to that for new pseudowords, suggesting that the learned codes for pseudowords are as strong and permanent as the codes for words. A model of identification is presented in which feedback from codes and episodic images in memory facilitates letter processing. An instantiation of the model accounts for the major features of the data.

Attention↗

Predicting similarity and categorization from identification.

In this article, the relation between the identification, similarity judgment, and categorization of multidimensional perceptual stimuli is studied. The theoretical analysis focused on general recognition theory (GRT), which is a multidimensional generalization of signal detection theory. In one application, 2 Ss first identified a set of confusable stimuli and then made judgments of their pairwise similarity. The second application was to Nosofsky's (1985b, 1986) identification-categorization experiment. In both applications, a GRT model accounted for the identification data better than Luce's (1963) biased-choice model. The identification results were then used to predict performance in the similarity judgment and categorization conditions. The GRT identification model accurately predicted the similarity judgments under the assumption that Ss allocated attention to the 2 stimulus dimensions differently in the 2 tasks. The categorization data were predicted successfully without appealing to the notion of selective attention. Instead, a simpler GRT model that emphasized the different decision rules used in identification and categorization was adequate.

Adult↗

Data-driven and conceptually driven processes in partial-word identification and recognition.

Three experiments examined priming in partial-word identification (Warrington & Weiskrantz, 1968) and its relation to recognition memory. The results showed that changes in modality of presentation between study and test reduced performance on both identification and recognition. In contrast, changes in elaborative processing enhanced recognition but had no effect on identification. Furthermore, when explicit memory instructions were given, identification was changed to a cued recall test and was consequently affected by elaborative processing. We also found that the time course of forgetting in priming was different from that in recognition; priming in identification did not change over a 24-hr interval, whereas recognition declined rapidly during this interval. Overall, these results suggest that identification relies primarily on data-driven processing, whereas recognition can rely on both data-driven and conceptually driven processing.

Adult↗

Odor identification in normal aging and early Alzheimer's disease: effects of retrieval support.

Odor sensitivity and identification were examined in normal aging and early Alzheimer's disease (AD). The aims were to investigate AD as associated with lower odor sensitivity, odor identification as a function of retrieval support, and the relationship between global cognitive functioning (Mini-Mental State Exam [MMSE]; M. F. Folstein, S. E. Folstein, & P. R. McHugh, 1975) and olfactory performance. Results indicated intact odor sensitivity but deficient odor identification in AD. Both groups benefited from cues in identification, and the size of the gains was equally large in AD patients and controls. The finding of no selective benefit from retrieval support in AD suggests that a degradation of olfactory knowledge contributes to the odor identification deficits in these patients. MMSE and identification were positively related, whereas MMSE and olfactory sensitivity were unrelated. These findings suggest that the AD-related olfactory impairment stems from lesions in cortical rather than peripheral structures.

Aged↗

Identification by DNA analysis of the victims of the August 1996 Spitsbergen civil aircraft disaster.

Disaster victim identification traditionally relies on the combined efforts of police, dentists and pathologists, comparing ante mortem (AM) information from the missing persons with post mortem (PM) data from the dead bodies. In Western countries, dental evidence has ordinarily played the major role. DNA analysis has been used successfully in a number of large accidents to associate body parts and for purposes of identification, by comparing victims' DNA profiles with those of relatives. However, DNA typing is still not generally regarded as an essential part of disaster victim identification. Facing the August 1996 Spitsbergen aircraft accident in which 141 Russians and Ukrainians died and anticipating scanty ante mortem dental data, it was decided to use DNA profile analysis as the primary identification method. Material collected at the scene from all body parts, and blood sample from relatives were analysed at eight polymorphic microsatellite and minisatellite loci, DNA profile comparisons enabled us to sort the 257 typed body parts into 141 individuals, as well as identifying the 139 victims for whom reference samples were available. Identification by DNA analysis was then followed by comparisons of traditional AM and PM data, and within day 20 of the accident the identities of all victims were confidently established. This investigation indicates that it might be feasible to replace traditional identification efforts with DNA typing.

Aircraft↗

Relationship between the discrimination of (w-r) and (t-d) continua and the identification of distorted (r).

The purpose of this study was to assess the extent to which listeners can perceive intraphonemic differences. In Experiment 1, subjects identified synthesized acoustic tokens of child-like speech that varied in second and third formant (F2 and F3) onset frequencies as (w), (r), or distorted (r) in two conditions: (a) with and without feedback of the group response choices, and (b) before and after training to identify the best examples of (w), (r), and distorted (r) based on their identification in the first condition. The results were: (a) some subjects consistently identified distorted (r) above criterion, and (b) feedback was more effective in increasing distorted (r) identification than was training. In Experiment 2, the same subjects participated in discrimination tasks using stimuli from a synthesized child (w-r) continuum that varied in F2 and F3 onsets and from a synthesized adult (t-d) continuum that varied in preconsonantal vowel duration. The results were: (a) perception was not categorical for both continua, (b) little relation was found between distorted-(r) identification and measures of (w-r) discrimination, and (c) a high and significant correlation was found between identification of distorted (r) and within-(d) discrimination. In Experiment 3, different subjects identified the child manifold stimuli and discriminated stimuli in a synthesized child (w-r) continuum and in a synthesized adult (t-d) continuum. The results were: (a) neither (w-r) or (t-d) perception was categorical although the former came closer than the latter in terms of individual subject performance, (b) there was a high and significant correlation between distorted-(r) identification and within-(r) discrimination of (w-r) stimuli, and (c) there were high and significant correlations between distorted-(r) identification and mean, cross-category boundary, and within-(t) discrimination of (t-d) stimuli.

Adult↗

Molecular identification of Anopheles minimus s.l. beyond distinguishing the members of the species complex.

Correct species identification is the starting point for studying the epidemiological role of vectors. Identification is mostly achieved using morphological criteria, but this fails when sibling species and species with overlapping morphological characters are involved. The problem with the identification of Anopheles minimus s. l., one of the most widespread malaria vectors in South-East Asia, is twofold: it is a complex of at least two isomorphic species, and based on morphology, members of the complex are difficult to distinguish from closely related species. An identification method was developed for An. minimus species A and C, and four related species, An. aconitus, An. pampanai, An. varuna and An. jeyporiensis. PCR-amplified internal transcribed spacer 2 (ITS2) ribonuclear DNA (rDNA) fragments were digested with restriction endonuclease BsiZI. Clear diagnostic banding patterns for the six species were obtained on agarose gels. Testing field-collected specimens from different regions in South-East Asia indicated that the technique will be applicable over a wide geographical area. From this it is clear that molecular identification has to focus not only on the species of complexes, but also on related species if they hamper the morphological identification of the 'sensu lato species'.

Animals↗

Identification of Malassezia species from patient skin scales by PCR-RFLP.

OBJECTIVE: This study was aimed at the development of a DNA-based procedure directly applicable to pathological skin scales and at the assessment of its value in rapid laboratory confirmation and identification of each of the seven Malassezia species. These lipophilic basidiomycetous yeasts in predisposed individuals are involved in pityriasis versicolor, seborrheic dermatitis, blepharitis, folliculitis, atopic dermatitis and fungemia. Standard identification procedures to species level are available, but so far no system for direct detection and characterization of Malassezia species in clinical specimens is available. METHODS: Malassezia DNA was extracted from pathological skin scales by a modified hexadecyltrimethylammonium bromide (CTAB) method and amplified by single and nested polymerase chain reaction (PCR), assays using the general fungal ITS 1/4 and 3/4 primers for amplification of sequences from the Malassezia major ribosomal DNA complex. Restriction fragment length polymorphism (RFLP) analysis of PCR products was used in subsequent species identification. DNA extracted from culture-positive skin scales was also tested by PCR and the RFLP patterns obtained were analyzed. RESULTS: A total of 36 isolates were tested. Distinct pure culture and skin-scale ITS 3/4 HinfI and AluI restriction patterns differentially identified M. furfur, M. globosa, M. restricta, M. sympodialis, M. pachydermatis, M. obtusa and M. slooffiae. Malassezia DNA was extracted from pathological skin scales and RFLP identified solitary and multiple Malassezia species in the same specimen. Molecular identification was confirmed by cultures and biochemical tests. Concurrent detection and identification of Candida and Yarrowia species was also feasible from skin scales. CONCLUSION: The proposed method, described for the first time, could provide a sensitive and rapid detection and identification system for Malassezia species, which may be applied to epidemiological surveys and routine practice.

Animals↗

Accuracy of computerized automatic identification of cephalometric landmarks.

Computerized cephalometric analysis can include both landmark identification and determination of linear or angular measurements. Although its use is time saving compared with a manual method, the accuracy of automatic landmark identification remains unclear. The purpose of this study was to evaluate the accuracy of a computerized automatic landmark identification system that used an edge-based technique. The technique divides the scanned cephalogram into 8 rectangular subimage regions. After the resolution of these subimages is reduced, the edges are detected and the landmarks are located automatically. Thirteen landmarks were selected for assessment on a set of 10 test cephalograms. The results showed that the errors between manual and computerized identification for landmarks were not significantly different (P > .05) for 5 of 13 landmarks: sella, nasion, porion, orbitale, and gnathion. These results suggest that the accuracy of computerized automatic identification is acceptable for certain landmarks only. Further studies to improve the accuracy of computerized automated landmark identification are needed.

Cephalometry↗

Quality assurance and good laboratory practice in the mycological laboratory--compilation of basic techniques for the identification of fungi.

The identification of microfungi by morphology is generally regarded to be very difficult, as traits used for delimitation of species within a genus often show minute differences that can only be reliably evaluated by experienced mycologists. Different strains of one species can slightly vary in their morphology. This is often used as an argument by investigators with rather limited experience that identification on a morphological basis is not reliable and leads to inconsistent results. This paper compiles essential methods and techniques contributing to improved identification concerning (i) isolation from mixed cultures, (ii) inoculation and incubation, (iii) usage of media, (iv) identification and usage of determination keys, (v) preparation of microscopic slides, (vi) microscopy, and (vii) storage of isolates. The application of chemotaxonomy and sequencing for routine identification of airborne microfungi is shortly evaluated. The techniques compiled here can improve routine determination work considerably and will lead to much more accuracy and consistency of results. An improved quality assurance of applied fungal identification in the field of indoor and occupational hygiene is a prerequisite to find possible correlations between exposure on the one hand and health effects on the other.

Clinical Laboratory Techniques↗

DNA-based methods for the identification of insect vectors.

Many insect vectors are members of complexes composed of morphologically identical sibling species. The identification of individual species, a requirement of epidemiological studies and control programmes, has traditionally relied upon techniques such as chromosomal analysis or isoenzyme typing. Owing to the limitations of these techniques, the last few years have seen many developments in DNA-based technologies for identification. DNA-based protocols have advantages over the other techniques utilized, in that they may identify all insect stages of both sexes using alcohol-preserved, dried, fresh or frozen specimens. The methods ultimately rely upon either DNA probe hybridization or the polymerase chain reaction (PCR). This review describes a number of approaches taken towards the development of these techniques. The aim of these approaches, whether directed or random, is to produce a methodology that is cheap, accurate and easy to use. In this review, the DNA-based techniques developed for the identification of Anopheles gambiae complex mosquitoes are used to illustrate the power of these methods, although, as the review demonstrates, the technology is directly applicable to many other mosquito or insect vectors. In addition, the methods discussed may be utilized for generating additional epidemiological data, such as identification of parasites within the vector or origin of the bloodmeal. A comprehensive survey of the probe systems available for the identification of insect vectors and the disease-causing organisms they transmit to the human population is therefore included. Given further advances in this technology, it may be anticipated that DNA-based approaches to identification may eventually supersede more traditional methodologies in the fields of tropical medicine and parasitology.

Animals↗

Psychological characteristics and talent identification in soccer.

I review research on psychological characteristics and sports performance and examine the literature on talent identification with particular reference to soccer to derive implications for the use of psychological variables in the talent identification and development process. Although the many cross-sectional studies of psychological characteristics and performance in all football codes conducted over the last 30 years have revealed no clear patterns, studies of both general inventories and specific variables are still being conducted. Reports on talent identification in all codes have increased in recent years, but most are descriptive in nature. In this review, I suggest that research on systematic expert observation has potential as a practical approach, but more studies of this type are needed. Considering the examination of specific psychological variables, only a solitary investigation of creativity in adolescents has shown promise. Further research on creativity and talent identification is required to replicate the positive results found in that study. In summarizing the research on psychological characteristics and talent identification, I conclude that cross-sectional research on adults cannot be extrapolated for use in talent identification with adolescents. I propose that resources would be more effectively used in the provision of psychological skills training for adolescent soccer players, pending more sophisticated research on a wider range of psychological variables. It is recommended that longitudinal or quasi-longitudinal research is essential to determine whether the same psychological variables are important for outstanding performance throughout the process of development and whether psychological variables measured during adolescence can predict outstanding performance in adulthood.

Humans↗

Yeast identification in the clinical microbiology laboratory: phenotypical methods.

Emerging yeast pathogens are favoured by increasing numbers of immunocompromised patients and by certain current medical practices. These yeasts differ in their antifungal drug susceptibilities, and rapid species identification is imperative. A large variety of methods have been developed with the aim of facilitating rapid, accurate yeast identification. Significant recent commercial introductions have included species-specific direct enzymatic colour tests, differential chromogenic isolation plates, direct immunological tests, and enhanced manual and automated biochemical and enzymatic panels. Chromogenic isolation media demonstrate better detection rates of yeasts in mixed cultures than traditional media, and allow the direct identification of Candida albicans by means of colony colour. Comparative evaluation of rapid methods for C. albicans identification, including the germ tube test, shows that chromogenic media may be economically advantageous. Accurate tests for single species include the Bichrolatex Albicans and Krusei Color tests, both immunologically based, as well as the Remel Rapid Trehalose Assimilation Broth for C. glabrata. Among broad-spectrum tests, the RapID Yeast Plus system gives same-day identification of clinical yeasts, but performance depends on inoculum density and geographic isolate source. The API 20 C AUX system is considered a reference method, but newer systems such as Auxacolor and Fungichrom are as accurate and are more convenient. Among automated systems, the ID 32 C strip, the Vitek Yeast Biochemical Card and the Vitek 2 ID-YST system correctly identify >93% of common yeasts, but the ID-YST is the most accurate with uncommon yeasts, including C. dubliniensis. Spectroscopic methods such as Fourier transformed-infrared spectroscopy offer potential advantages for the future. Overall, the advantages of rapid yeast identification methods include relative simplicity and low cost. For all rapid methods, meticulous, standardized multicenter comparisons are needed before tests are fully accepted.

Candida↗

Cultural identification and alcohol use among "Black" adolescents.

This study examined the relationship between cultural identification and alcohol use among Black adolescents--77 African-American and 185 Haitian Black adolescents. The Orthogonal Cultural Identification Scale assessed cultural identification. A logistic regression analysis was used to evaluate the association between cultural identification association between cultural identification, friendships, and alcohol use. Results indicate no relationship between cultural identification and alcohol use. Drinking during the past six months was significantly associated with having close friends who drink. Although the direction of friend influence is unclear. Differences were observed in the context of drinking between the two groups and, importantly, youths in both groups reported drinking alone and to relax/because of stress. Sociocultural factors, context and the basis of drinking need to be addressed in programs for youth.

Adolescent↗

System identification: a multi-signal approach for probing neural cardiovascular regulation.

Short-term, beat-to-beat cardiovascular variability reflects the dynamic interplay between ongoing perturbations to the circulation and the compensatory response of neurally mediated regulatory mechanisms. This physiologic information may be deciphered from the subtle, beat-to-beat variations by using digital signal processing techniques. While single signal analysis techniques (e.g., power spectral analysis) may be employed to quantify the variability itself, the multi-signal approach of system identification permits the dynamic characterization of the neural regulatory mechanisms responsible for coupling the variability between signals. In this review, we provide an overview of applications of system identification to beat-to-beat variability for the quantitative characterization of cardiovascular regulatory mechanisms. After briefly summarizing the history of the field and basic principles, we take a didactic approach to describe the practice of system identification in the context of probing neural cardiovascular regulation. We then review studies in the literature over the past two decades that have applied system identification for characterizing the dynamical properties of the sinoatrial node, respiratory sinus arrhythmia, and the baroreflex control of sympathetic nerve activity, heart rate and total peripheral resistance. Based on this literature review, we conclude by advocating specific methods of practice and that future research should focus on nonlinear and time-varying behaviors, validation of identification methods, and less understood neural regulatory mechanisms. Ultimately, we hope that this review stimulates such future investigations by both new and experienced system identification researchers.

Algorithms↗

Comparison of two methods for same-day identification of Enterobacteriaceae.

Two commercial methods, API 20E (as modified for same-day enterobacterial identification) and Micro-ID, were evaluated for ability to provide useful same-day information of 368 clinically isolated Enterobacteriaceae. Organisms included Escherichia coli (54), Shigella (7), Edwardsiella tarda (1), Salmonella enteritidis (10), Citrobacter (30), Klebsiella (55), Enterobacter (68), Hafnia alvei (2), Serratia (33), Proteus (64), Morganella morganii (24), Providencia (18), and Yersinia enterocolitica (2). Methods were those of manufacturers without supplemental tests. API at five hours identified 78.5% of strains to species, 9.5% to genus only, 10.1% as part of a spectrum of identifications (SI), and 1.9% incorrect. Micro-ID at four hours yielded 90.0% correct identification to species and 3.3% to genus only, 4.0% SI, and 2.7% incorrect. API identification of many Serratia, Citrobacter, Providencia strains was to genus only; most incorrect results occurred in Serratia marcescens. Micro-ID identified most organisms to species; incorrect identifications were mainly S. marcescens and Klebsiella pneumoniae. Both systems provided excellent identification of E. coli. Both methods sacrifice a degree of accuracy that varies with the species tested, as compared to overnight systems, but both provide rapid information of potential clinical value.

Culture Media↗

Evaluation of the IDS RapID SS/u system for rapid identification of urinary microorganisms.

The accuracy of a new rapid identification system for common urinary pathogens was compared with that of conventional methods and of miniaturized 18-24-hour identification panels. The rapid system, RapID SS/u (Innovative Diagnostic System Inc., Atlanta, GA) is a non-growth-dependent micro-method that identifies selected gram-negative bacilli, gram-positive cocci, and yeasts in two hours by detection of constitutive enzymes acting on chromogenic substrates. A total of 185 representative clinical urinary isolates were tested, including 24 gram-positive cocci, 140 gram-negative bacilli, and 21 yeasts. Identifications by the rapid system were compared with the ones obtained by reference conventional methods for gram-positive cocci and yeasts. For gram-negative bacilli, identifications were compared with the ones obtained by MicroScan Combo Panel (American MicroScan, Mahwah, NJ), and all discrepancies were resolved by testing with API 20E (Analytab Products, Plainview, NY). Overall, the RapID SS/u system correctly identified to genus 160 of 185 isolates (86.5%). For 14 additional isolates (7.6%) the system provided probability overlap identifications that required further testing. Two (1%) isolates failed to be identified, and nine isolates (4.9%) were misidentified by the system. Discrepancies involved five strains of Citrobacter, one Enterobacter, one Morganella, and one Providencia species. The authors conclude that the RapID SS/u system provided rapid and accurate genus identification of most microorganisms commonly isolated from urine.

Humans↗

Genome analysis technologies: towards species identification by genotype.

Traditional identification of species has been based on phenotypic traits, although it is clear that, theoretically, genotype-based classification is more accurate. This is especially the case for microorganisms which possess less identifiable traits and are more easily influenced by environment. Therefore, technology that allows identification of species based on genotype is highly desirable. Whole genome sequencing can provide a sufficient amount of information and can be determinative for this purpose but is very impractical for routine use. Thus, a competent technology is needed that allows a reproducible reduction in the amount of information required about a whole genome, while still providing sufficiently accurate identification. It is almost imperative for such a technology to be of a high cost-performance and of easy handling. Universality and portability are also strongly desired. Based on these criteria, the current state of genome analysis technologies are reviewed. Among various methodologies discussed here, amplified fragment length polymorphism (AFLP), genome profiling (GP) and microarrays are the subject of particular attention. As species identification is a base for most fields of biology including microbiology, ecology, epidemiology and for various biotechnologies, it is of paramount importance to establish a more efficient, easily handled and more objective methodology, in parallel with conventional phenotype-based methodologies. GP is currently considered to have the most optimal nature for identification of species since it can reproducibly reduce a huge amount of genome information to a manageable size by way of random polymerase chain reaction and can extract a sufficient amount of information for species identification from the DNA fragments thus profiled by temperature gradient gel electrophoresis. The potential ability of DNA microarrays for this purpose is also discussed and promises much for the future.

Genome↗