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PubMed2006

[Vitreal fluorometry].

Vitreous fluorophotometry is an investigation that facilitates the quantitative study of the blood-retinal barrier (thus allowing a better understanding of its physiopathology and opening new therapeutical research fields). It can be performed for the vast majority of the ocular diseases: diabetic retinopathy, arterial hypertension, uveitis, post-cryotherapy/laser photocoagulation, retinal vascular diseases, choroidal melanoma, optic nerve diseases etc.

Blood-Retinal Barrier↗
PubMed1991

[An evaluation of the conformational changes in the superhelical DNA of eukaryotic cells by direct nucleoid fluorometry. II. The characteristics of the change in acridine orange fluorescence in studying the superhelical DNA of rat thymocytes].

Comparative studies of acridine orange (AO) and ethidium bromide interactions with supercoiled DNA (scDNA) of thymocytes were performed in which various conformational changes were induced. AO may be efficiently used for evaluation of conformational alterations of scDNA. Moreover, employing the maximum values of AO fluorescence allows to determine the maximum levels of scDNA relaxation.

Acridine Orange↗
PubMed1990

Ultrasensitive two-site immunometric assay of human lutropin by time-resolved fluorometry.

A rapid, sensitive two-site immunometric assay for human lutropin (LH), with two monoclonal antibodies directed against the beta subunit, has been developed with time-resolved fluorescence as the detection principle. The cross-reactivities with human thyrotropin and human follitropin are negligible and that with human choriogonadotropin is 1% in the standard two-step protocol. Free beta subunits of LH are also detected. Besides the standard procedure (45 + 15 min), more rapid alternative protocols (15 + 15 min or 30 min in one step) have also been evaluated. The minimum detectable dose per well is approximately 1 amol (10(-18) mol), corresponding to less than 0.01 int. unit/L, for a sample volume of 25 microL and less than 0.001 int. unit/L for 200-microL samples. The intra- and interassay CV is less than 6% for LH concentrations from 0.2 to 250 int. units/L. With 200-microL sample volumes, intra-assay variation is less than 6% at 0.04 int. unit/L and interassay variation is 7% at 0.05 int. unit/L. The very high sensitivity and reproducibility of the presented method enables assay of low prepubertal and of suppressed LH concentrations, and represents a clear improvement over currently available immunoassays.

Adult↗
PubMed1979

Manual fluorometry of phenylalanine from blood specimens collected on filter paper: a modified procedure.

In this manual fluorometric method, blood samples are used that have been impregnated on the filter paper, a convenient collection technique that is widely used to screen newborns for phenylketonuria. The modified procedure, based on the method of McCaman and Robins [J. Lab Clin. Med. 59, 885 (1962)], includes elution of phenylalanine from specimens on filter paper, removal of proteins by precipitation with trichloroacetic acid at 0 degrees C, and then reaction with ninhydrin-peptide reagent for color development. The standard curve is linear to at least 200 mg/L and the CV is 6.3% for a phenylalanine concentration of 27 mg/L. The modified procedure is suitable both for early screening for phenylketonuria and for monitoring blood phenylalanine of phenylketonurics during dietary therapy.

Blood Specimen Collection↗
PubMed1988

Evaluation of cytoprotective drugs for liver preservation by pyridine nucleotide fluorometry.

The cytoprotective effects of membrane-stabilizing drugs, such as chlorpromazine, allopurinol, dibucaine, phenoxybenzamine, and OP41483 (prostacyclin analogue), administered to perfusate and preservation medium were studied in rat liver, after 24 hours' preservation, by assessment of pyridine nucleotide fluorescence. On the fluorometric trace curve, amplitude (RxA) and velocity (RxV) from oxidation to reduction were determined. Percent decrease of RxA (%RxA) and that of RxV (%RxV) after 24 hours' preservation were calculated. At the end of preservation, the concentration of total adenine nucleotides of the liver, hepatic adenylate energy charge, and prepared mitochondrial oxidative phosphorylative activity were also measured. In the groups given phenoxybenzamine, dibucaine, and allopurinol, there was no significant difference among these parameters. In the chlorpromazine group, energy charge and %RxV were higher than in the drug-free group (p less than 0.05). In the OP41483 group, both energy charge and phosphorylation rate were significantly higher (p less than 0.05) and %RxV was significantly high (p less than 0.01) at concentrations of more than 3 nmol/L, compared with the values for those without drugs. These results suggest that the Redoximeter can provide accurate information on the effectiveness of cytoprotective drugs. It is also suggested that OP41483 has potential application for maintaining graft viability for human liver transplantation.

Allopurinol↗
PubMed1988

Resolution of the lifetimes and correlation times of the intrinsic tryptophan fluorescence of human hemoglobin solutions using 2 GHz frequency-domain fluorometry.

We used 2 GHz harmonic content frequency-domain fluorescence to measure the intensity and the anisotropy decays from the intrinsic tryptophan fluorescence from human hemoglobin (Hb). The tryptophan intensity decays are dominated by a short-lived component which accounts for 35-60% of the total steady state intensity. The decay time of this short component varies from 9 to 27 ps and this component is sensitive to the ligation state of Hb. Our error analyses indicate the uncertainty is about +/- 3 ps. The intensity decays also show two longer lived components near 0.7 and 8 ns, which are probably due either to impurities or to Hb molecules in conformations which do not permit energy transfer. The anisotropy decays indicate the tryptophan residues in Hb are highly mobile, with apparent correlation times near 55 ps.

Carboxyhemoglobin↗
PubMed1986

Three-dimensional fluorometry for the detection of DNA adducts.

We describe the interfacing of a fluorometer to a desk-top computer by means of a commercially available interface box, for the purpose of generating three-dimensional fluorescence spectra. The important features of a self-designed program in BASIC are discussed in detail.

DNA↗
PubMed1977

[Quantitative fluorometry at the ocular fundus (author's transl)].

Description of a videodensitometric method for the quantitative evaluation of fluorescence angiography photographs using the Quantimet 720 image analysis computer system. The clinical value of the new method is demonstrated in a case of central serous retinopathy. With the aid of these measurements the activity of leakage points can be assessed more exactly than by mere subjective inspection of the gained pictures, thus allowing a better control of the effects of a chosen therapy.

Computers↗