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At least 505 records · Page 28Linked to original sources
PubMed1978

Potential sources of error during virus thermal inactivation.

A review of virus thermal inactivation data published in the literature demonstrated variations in reported virus resistance. Examination of the methods used indicated that numerous studies were made by heat processing virus suspensions in test tubes. Duplication of some of the methods using milk suspensions of poliovirus 1 showed virus persistence after heating as a result of uneven temperature distribution inside the test tubes. Unless the containers (preferably sealed ampoules or capillary tubes) are completely submerged in the water bath and agitated vigorously, apparent virus persistence may be encountered.

Animals↗
PubMed1988

Sources of error in neonatal blood sampling.

Significant underestimation of blood electrolyte concentrations may occur if less than 1 ml of blood is withdrawn from an indwelling arterial line before taking the sample for analysis. Compared with arterial samples capillary samples give significant overestimations of potassium concentration and carbon dioxide tension, and significant underestimation of oxygen tension.

Arteries↗
PubMed1979

Accuracy of subjective measurements made with or without previous scores: an important source of error in serial measurement of subjective states.

Pain severity was assessed on visual analogue scale in 4 groups of patients receiving long-term therapy. A comparison was made between pain measurements made with and without access to initial scores. The differences between these measurements increased with the duration of treatment. Patients tended to overestimate their pain severity when previous scores were not available. It is suggested that initial scores should be made available when serial measurements of pain are made in long-term experiments.

Humans↗
PubMed1976

Defects in sphygmomanometers: an important source of error in blood pressure recording.

Almost half the sphygmomanometers in a teaching hospital group had defects in the control valve which interfered with accurate blood-pressure reading. Ward staff should be taught to check sphygmomanometers regularly and replace control valves; time consumption and cost are low. The cuffs in general use in these hospitals, and of the standard size sold in Britain, had rubber bags which did not encircle the arm of more than half the patients on whom they were used. This deficiency causes over-reading of blood pressure in obese people. The size of error is uncertain but it should be avoided by adopting a cuff with a longer bag.

Adolescent↗
PubMed1988

Variability in vibration perception threshold among sites: a potential source of error in biothesiometry.

Vibration perception threshold was measured with a biothesiometer by a single observer at both medial malleoli and both big toes in 110 diabetic patients aged 15-65 selected at random and in 64 non-diabetic subjects aged 20-65. The vibration perception threshold showed appreciable individual variation both between contralateral sites and between ipsilateral sites, differing by at least 30% between the big toes in 26 (24%) of the diabetic patients and 16 (25%) of the non-diabetic group. Variability between sites was significantly greater in the diabetics than the normal subjects. The vibration perception threshold exceeded published normal values at one or more sites in 22 of the diabetic patients but at all four sites in only four. The wide variability in vibration perception threshold among sites may be due to the tissue characteristics locally and, in diabetic patients, possibly to asymmetric neuropathy. Biothesiometer readings at single or unilateral sites may be unrepresentative or misleading.

Adolescent↗
PubMed1980

Detection of monoclonal immunoglobulins by immunoelectrophoresis: a possible source of error.

The technique of immunoelectrophoresis (IEP) is widely employed in the qualitative analysis of serum immunoglobulins. The most commonly used support media are agarose or agar gels, but the mobility of immunoglobulins is different in these two media. The presence of a small amount of a cathodal monoclonal immunoglobulin G may not be detected on IEP in agar if it is masked by larger amounts of polyclonal immunoglobulin of the same class. In these circumstances the use of agarose imparts to the monoclonal protein a different mobility from that of the bulk of the serum IgG and allows its positive identification.

Agar↗
PubMed1989

Alpha thalassaemia: a potential source of error in DNA linkage studies for adult polycystic kidney disease.

DNA polymorphisms associated with adult polycystic kidney disease and detected by restriction enzymes PvuII and RsaI were sought in 18 members of a Greek Cypriot family. The adult polycystic kidney disease (APKD) defect was shown to segregate with a specific 3'HVR PvuII DNA polymorphism and enabled exclusion of APKD in two children incorrectly diagnosed by ultrasound. An unusual problem of the DNA linkage approach in detection of APKD is illustrated. This occurred after coinheritance of alpha+ thalassaemia and APKD, which resulted in an altered band size for the (+) RsaI restriction fragment length polymorphism.

Adolescent↗