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PubMed1991

The effect of pure and partial yellow endosperm sorghum grain hybrids on site and extent of digestion in beef steers.

To compare the effects of sorghum grain hybrids on site and extent of digestion, two yellow (Y1 and Y2), two cream (C1 and C2), and two hetero-yellow (HY1 and HY2) sorghum grains were fed (1.85% BW, DM basis) in an 81% dry-rolled grain diet to steers (342 kg BW) equipped with ruminal, duodenal, and ileal cannulas within a 6 X 6 Latin square. Yellow (YEL) hybrids had a homozygous yellow endosperm and a yellow seed coat; cream (CREAM) and hetero-yellow (HET-YEL) hybrids had a heterozygous (partial) yellow endosperm, with white or red seed coats, respectively. Total tract starch digestibility (percentage) was greater (P less than .10) for CREAM and HET-YEL (82.3) than for YEL (78.9), primarily because of greater (P less than .05) starch digestion in the large intestine. Ruminal starch digestibility (percentage) was greater (P less than .10) for HET-YEL (73.2) than for CREAM (66.3) and was a larger proportion of total tract digestion for HET-YEL (90.6) than for CREAM (80.1). Ruminal starch digestion was correlated negatively (r = -.46; P less than .08) with ruminal escape of feed N. Prececal starch digestibility (average 76.2%) was more strongly correlated with ruminal digestibility (r = .69; P less than .01) than with digestion in the small intestine (r = .41; P = .12). Total tract nonammonia N (NAN) digestibility (percentage) was greater (P less than .10) for CREAM than for HET-YEL, greater for Y1 (P less than .10) than for Y2, greater for C2 (P less than .05) than for C1, and greater for HY2 (P less than .05) than for HY1. Flow of NAN to the duodenum was correlated negatively (r = -.55; P less than .05) with prececal starch digestion. Small intestinal NAN disappearance (g/d) was greater (P less than .01) for HY1 (76.0) than for HY2 (52.2). Microbial N flow (r = .88; P less than .01), but not feed N flow (r = .17; P = .52), to the duodenum was correlated with partial NAN digestibility in the small intestine. Hybrids differed in site and extent of digestion. Differences were generally larger for N than for starch.

Animal Feed↗
PubMed1995

Effect of supplemental fish meal protein on site and extent of digestion in beef steers.

The effects of supplemental fish meal on site and extent of digestion were determined using four steers equipped with ruminal, duodenal, and ileal cannulas. Fish meal was included in diets to supply 0 (0FM), 25 (25FM), 50 (50FM), or 75 (75FM) g of N daily above the CP requirement of a 400-kg steer gaining 1.2 kg/d. Total tract starch digestibility tended to be greatest for 25FM (95%), lowest for 0FM (90%), and intermediate for 50FM (94%) and 75FM (92%). Total tract N digestibility was greatest for 25FM, lowest for 0FM, and intermediate for 50FM and 75FM (cubic, P < .05). Total tract starch digestibility increased .46 percentage units for each unit increase in N digestibility (n = 16, P = .03). Ruminal starch digestibility was greatest for 25FM, intermediate for 50FM and 75FM, and lowest for 0FM (quadratic; P < .10). Starch flow to the duodenum was decreased for 25FM and 50FM vs 0FM and 75FM (quadratic, P < .10) and N flow to the duodenum was increased (linear, P < .01) by fish meal. Fish meal supplementation increased N digestibility (quadratic, P < .05) but had no effect (P > .10) on starch digestibility in the small intestine. Starch digestibility in the small intestine was not related to N flow to the duodenum; however, starch digestibility increased .9 percentage units for each percentage unit increase in N digestibility (n = 16, P = .02). Fish meal supplementation tended to increase total tract starch digestibility by increasing ruminal, but not small intestinal, digestibility.

Animals↗
PubMed1999

Comparison of Tifton 85 and Coastal bermudagrasses for yield, nutrient traits, intake, and digestion by growing beef steers.

A study was undertaken to compare Tifton 85 (T85) and Coastal (CBG) bermudagrasses for effects of cultivar and age at harvest on yields of DM and digestible DM, in vitro digestion, nutrient content, cell wall composition, in situ digestion kinetics, and feed intake and digestion by growing beef steers. In Exp. 1, T85 and CBG forages staged for growth in May or July of 1993 were harvested at 3, 4, 5, 6, 7, and 8 wk from subplots. Tifton 85 bermudagrass had 7.1% greater DM yield, 18.2% higher (P < .05) digestible DM yield, and 7.1% greater IVDMD than CBG, and, after 5 wk of forage growth, IVDMD of both T85 and CBG decreased with increased age at harvest (P < .05). In Exp. 2, T85 and CBG forages staged for growth in July 1997 were harvested at 2, 3, 4, 5, 6, and 7 wk from subplots. Even though T85 had higher concentrations of NDF and ADF than CBG, T85 had 34.1% higher DM yield, 47.9% higher digestible DM, 55.0% higher digestible NDF, 91.7% higher digestible ADF, greater IVDMD, in vitro NDF and ADF disappearances, and higher in situ DM and NDF digestion (P < .05). Coastal bermudagrass had higher concentrations of lignin and lower concentrations of total neutral sugars, arabinose, glucose, and xylose than T85 (P < .05). In vitro digestibilities of DM, NDF, and ADF were lower and concentrations of ADF and lignin were greater for 7- vs 6-wk harvests of both T85 and CBG (P < .05). In Exp. 3, T85 and CBG forages staged for growth in July 1997 were harvested as hay at 3, 5, and 7 wk from .8-ha pastures and fed to 36 individually penned growing beef steers (initial BW = 244 kg) to quantify ad libitum intake without supplementation. Tifton 85 bermudagrass had lower concentrations of lignin and ether-linked ferulic acid and greater concentrations of NDF, ADF, hemicellulose, and cellulose than CBG (P < .05). Steers fed T85 had higher (P < .05) digestion of DM, OM, NDF, ADF, hemicellulose, and cellulose than steers fed CBG. Digestion of NDF, ADF, hemicellulose, and cellulose decreased (P < .05) with increased age at harvest for both cultivars. In conclusion, T85 produced more DM and had more digestible nutrients in vitro, in situ, and in vivo than CBG, and 3 and 5 wk of growth would be recommended ages to harvest either cultivar.

Animal Nutritional Physiological Phenomena↗
PubMed2004

Effect of supplementation of xylanase and phospholipase to a wheat-based diet for weanling pigs on nutrient digestibility and concentrations of microbial metabolites in ileal digesta and feces.

The objective of this study was to determine the effect of supplementing a wheat-based diet with xylanase and phospholipase either alone or in combination on the ileal and fecal digestibilities of nutrients and energy in early-weaned pigs. In addition, the concentrations of ammonia, lactate, and VFA were measured in ileal digesta and feces. The experiment was carried out with 16 barrows weaned at the age of 11 d with an average initial BW of 4.1 kg. On d 4 and 5 postweaning, the piglets were fitted with a simple T-cannula at the distal ileum. The experiment was designed as a balanced incomplete block design with three periods. The piglets received the basal diet with or without supplementation of either xylanase or phospholipase or a combination of these. There was a positive (P = 0.005 to 0.018) effect on the digestibility values of GE, OM, CP, crude fiber (CF), and NDF with xylanase supplementation. Apart from lysine, threonine, cysteine, glycine, and proline, the digestibility values of all AA were improved (P = 0.001 to 0.024). Phospholipase supplementation had a positive effect on CP (P = 0.047) and CF (P = 0.002) digestibilities, but no effect on ether extract (EE) digestibility. Supplementation of both enzymes showed the largest response in nutrient digestibilities, except that EE digestibility was not affected. No differences were found in D-/L- lactate, and ammonia concentrations among treatments. Acetate and propionate concentrations tended to increase when xylanase was supplemented and were highest for the combination of both enzymes. Despite the positive effects on ileal nutrient and energy digestibilities, there was no effect of xylanase or the combined enzyme supplementation on the fecal digestibilities of OM, CP, EE, CF, NDF, ADF, or GE, and on fecal concentrations of VFA. Phospholipase alone slightly decreased the total-tract nutrient and energy digestibilities (P < 0.05). In conclusion, the combination of both enzymes generally led to the highest increases in ileal digestibilities, which were of small numerical magnitude (approximately 2%). However, on a relative basis, this increase of 2% represents approximately 13% of the remaining diet that was available for digestion based on the fact that approximately 15% of the diet was not digested in the control pigs. Thus, the potential benefits in the nutrition of weanling pigs from combinations of enzymes should be validated under practical conditions.

Animals↗
PubMed2005

Corn hybrid affects in vitro and in vivo measures of nutrient digestibility in dogs.

Corn is a commonly used ingredient in dry pet foods because there is a stable supply and it is a relatively inexpensive source of nutrients. Corn hybrids are available that are higher in CP and amylose and lower in phytate concentration than conventional hybrids. Approximately 500 mg of high-protein (HP), high-protein, low-phytate (HPLP), and high-amylose (HA) corn were compared with conventional (CONV) corn and amylomaize starch (AM) in triplicate and exposed to pepsin/hydrochloric acid and pancreatin to simulate hydrolytic digestion. Substrate remaining after this was used to determine in vitro colonic fermentation. Organic matter disappearances as a result of hydrolytic digestion were >80% for CONV, HP, and HPLP, whereas HA (60.7%) and AM (43.7%) were lower (P < 0.05). Total digestion (TD) values after hydrolytic digestion and 8 h of fermentation using canine fecal inoculum were greater (P < 0.05) for CONV, HP, and HPLP vs. HA and AM. The residue left after hydrolytic digestion of all substrates was poorly fermented. Five ileal-cannulated dogs were fed each corn hybrid at approximately 31% of the diet in a 5 x5 Latin square design. Dogs fed diets containing HP corn had higher (P < 0.05) ileal OM digestibility (70.3%) and tended (P < 0.10) to have higher DM digestibility (64.6%). Ileal starch digestibilities were lower (P < 0.05) for dogs fed HA (64.0%) and AM (63.0%). Ileal digestibilities of essential (71.2%), nonessential (67.4%), and total (69.0%) AA tended to be higher (P < 0.10) for HP diets compared with CONV (66.4, 62.4, and 64.0%, respectively). Total-tract DM, OM, CP, and GE digestibilities (77, 82, 77, and 84%, on average, respectively) were higher (P < 0.05) for dogs fed CONV, HP, and HPLP than for those fed AM (66.9, 71.6, 72.6, and 76.5%) and HA (60.6, 65.7, 69.7, and 71.5%). Total-tract fat digestibilities were lower (P < 0.05) for dogs fed HA diets (86.6%) than for all other treatments (91.0%, on average). Total-tract starch digestibilities were higher (P < 0.05) for dogs fed CONV, HP, and HPLP (98%, on average) compared with HA (72.8%) and AM (76.5%). No differences were detected among treatments in fecal bifidobacteria, lactobacilli, or Clostridium perfringens concentrations. The experiments demonstrated that HP and HPLP corn had hydrolytic digestion and fermentation characteristics similar to those of CONV corn, whereas HA resulted in similar responses to AM, a well-established resistant starch ingredient.

Acids, Acyclic↗
PubMed2006

Sites, rates, and limits of starch digestion and glucose metabolism in growing cattle.

Growing cattle in the United States consume up to 6 kg of starch daily, mainly from corn or sorghum grain. Total tract apparent digestibility of starch usually ranges from 90 to 100% of starch intake. Ruminal starch digestion ranges from 75 to 80% of starch intake and is not greatly affected by intake over a range of 1 to 5 kg of starch/d. Starch apparently digested in the small intestine decreases from 80 to 34% as starch entering the small intestine increases from 0.2 to 2 kg/d. Starch apparently digested in the large intestine ranges from 44 to 46% of starch entering the large intestine. Approximately 70% of starch digested in the small intestine appears as glucose in the bloodstream. Within the range of starch intakes that do not cause rumen upsets, increasing starch (and energy) intake increases the amount of starch digested in the rumen, increases the supply of starch to the small intestine, increases starch digested in small intestine (albeit at reduced efficiency), and increases starch digested in the large intestine, such that total tract digestibility remains relatively constant. With increased starch intake, most of the starch is still digested in the rumen, but increasing amounts of starch escape ruminal and intestinal digestion, and disappear distal to the ileocecal junction. Again, within the range of starch intakes that do not cause rumen upsets, as starch intake increases, hepatic gluconeogenesis increases, glucose entry increases, and glucose irreversible loss increases, with a significant portion lost as CO2. The ability to increase use of dietary starch to support greater weight gains or improved marbling could come from increasing starch digestion in a healthy rumen or in the small intestine, but we conclude that the main limit to use of dietary starch to support live weight gain is digestion and absorption from the small intestine. Increased oxidation of glucose at greater starch intakes may alter energetic efficiency by sparing other oxidizable substrates, like amino acids.

Animals↗
PubMed1980

The effect of starch on forage fiber digestion kinetics in vitro.

Purified corn and wheat starch were added to alfalfa, Coastal bermudagrass, fescue, and orchardgrass hays at 0, 40, 60, and 80% of the total as-fed substrate, and fiber digestion kinetics were determined in vitro. Kinetics were estimated by the model R = Doe-k(t-L) + U where R is residue remaining at time t, Do is digestible fraction, k is digestion rate constant, L is discrete lag time, and U is indigestible fraction. Parameters of the model were estimated by logarithmic transformation and a direct nonlinear least squares procedure. Corn and wheat starch did not differ in their effect upon lag time of fiber digestion, digestion rate, or potential extent of digestion. Alfalfa had a shorter lag time of fiber digestion (.86 h) than Coastal bermudagrass (3.05 h), but not than orchardgrass or fescue (1.66 and 2.42 h). Orchardgrass differed in fiber digestion rate (.0542h-1) from Coastal bermudagrass (.0698h-1) but not from alfalfa or fescue (.0670 and .0658h-1). The potential extent of fiber digestion was similar for fescue (75.8%) and orchardgrass (76.0%). The potential extent of fibre digestion for alfalfa (50.9%) differed from Coastal bermudagrass (64.3%), and both of these forages differed from fescue or orchardgrass. Addition of starch resulted in a linear increase in lag time of fiber digestion, but digestion rate was not affected. Potential extent of digestion was decreased when starch was added.

Animal Feed↗
PubMed1997

Isolation and morphofunctional characterization of mussel digestive gland cells in vitro.

The aim of the present study was to isolate and characterize digestive gland cells of the bivalve mollusc Mytilus galloprovincialis Lmk. The digestive gland of bivalves is a complex organ composed of digestive and connective tissues but it is also invaded by the reproductive tissue as gametogenesis proceeds. The digestive tissue is comprised of stomach and intestinal epithelial cells, ciliated and non-ciliated duct cells, digestive cells and basophilic cells. the last two cell types are found lining the epithelium of the blind-ending digestive tubules and are the main focus of this study. Two different approaches were assayed for cell isolation, i.e., explant culture techniques and mechanical plus enzymatical digestion techniques. Cell viability was tested by trypan blue exclusion, neutral red uptake and ultrastructural analysis. The explant cultures were often contaminated with bacteria and spermatozoa and, moreover, cells migrating out of the explants possibly corresponded to hemocytes and not to digestive tissue cells. Mechanical plus enzymatical digestion with collagenase was concluded to be the method of choice for digestive cell isolation, with a percentage of about 30% of isolated cells corresponding to digestive cells. The ultrastructure of digestive cells isolated with the latter procedure closely resembled that of mussel digestive cells in vivo.

Animals↗
PubMed2005

Phenols in anaerobic digestion processes and inhibition of ammonia oxidising bacteria (AOB) in soil.

This study focuses on the presence of phenols in digestate from seven Swedish large-scale anaerobic digestion processes and their impact on the activity of ammonia oxidising bacteria (AOB) in soil. In addition, the importance of feedstock composition and phenol degradation capacity for the occurrence of phenols in the digestate was investigated in the same processes. The results revealed that the content of phenols in the digestate was related to the inhibition of the activity of AOB in soil (EC(50)=26 microg phenols g(-1) d.w. soil). In addition, five pure phenols (phenol, o-, p-, m-cresol and 4-ethylphenol) inhibited the AOB to a similar extent (EC(50)=43-110 microg g(-1) d.w. soil). The phenol content in the digestate was mainly dependent on the composition of the feedstock, but also to some extent by the degradation capacity in the anaerobic digestion process. Swine manure in the feedstock resulted in digestate containing higher amounts of phenols than digestate from reactors with less or no swine manure in the feedstock. The degradation capacity of phenol and p-cresol was studied in diluted small-scale batch cultures and revealed that anaerobic digestion at mesophilic temperatures generally exhibited a higher degradation capacity compared to digestion at thermophilic temperature. Although phenol, p-cresol and 4-ethylphenol were quickly degraded in soil, the phenols added with the digestate constitute an environmental risk according to the guideline values for contaminated soils set by the Swedish Environmental Protection Agency. In conclusion, the management of anaerobic digestion processes is of decisive importance for the production of digestate with low amounts of phenols, and thereby little risks for negative effects of the phenols on the soil ecosystem.

Ammonia↗
PubMed2001

Effect of a controlled-release urea supplementation on feed intake, digestibility, nitrogen balance and ruminal kinetics of sheep fed low quality tropical forage.

Four ruminally cannulated crossbred sheep (25+/-3.4kg BW) were divided into a 4x4 Latin square design to measure the effects of controlled-release urea supplement (CRUS). The basal diet consisted of 60% sugar cane tops (Saccharum officinarum), 30% full plant corn stubble (Zea mays), and 10% King grass (Pennisetum purpureum). Feed intake, digestibility, N balance and in situ ruminal kinetics were studied with four diets, D1 (control), D2, D3 and D4, which included the ratios of 100:0%, 90:10%, 80:20% or 70:30% of basal diet with CRUS. Results showed DMI differences (P<0.05) for D4 (822g per day) versus D1, D2 and D3 (580, 659 and 700g per day, respectively). N retention increased (P<0.05) for D4 (35.69g per day) versus D1, D2 and D3 (9.29, 6.85 and 19.10g per day, respectively). In vivo N digestibility was greater (P<0.05) in D4 (79.63%) than in D1 (57.57%). In vivo digestibility of DM, OM, GE, cellulose and hemicellulose was similar among the four groups. Digestibility of cell walls in D4 was higher (P<0.05) at 74.06% versus 67.78% in D1. In situ DM digestibility showed differences (P<0.05) among all diets at 9, 12, 24 and 48h of incubation. Potentially digestible fiber, 52.61%, was higher (P<0.05) in D4 versus 31.00% in D1. Indigestible fiber, 35.29%, was lowest (P<0.05) in D4 compared to 81.51% in D1. Digestion rate constant (k(d)) was different (P<0.05) between the experimental diets and control. Passage constant (k(p)) was different (P<0.05) between all diets (0.036/h in D4 to 0.081/h in D1). True digestibility was higher (P<0.05) in D4 (44.64%) compared to D1 (19.55%), but in D2 (24.54%) and D3 (28.22%) there was no difference. Cellulose in situ digestion rate, the potentially digestible fiber, was higher (P<0.05) in D3 (42.74%) as compared to D1 (22.50%). Time of disappearance of cellulose in D4 (14.79h) was less (P<0.05) than in D1 (24.03h), however there was no difference between D1 and D2. Hemicellulose in situ digestion was different (P<0.05) between D3 (45.48%) and D1 (23.61%). Digestion rate was higher (P<0.05) between D3 and D4 as compared to D1. Passage rate was different (P<0.05) between D4 (0.033/h) and D1 (0.018/h). True digestibility in D3 (34.84%) and D4 (34.62%) was higher (P<0.05) than that in D1 (20.06%) and D2 (25.86%). Half-time disappearance (t(1/2)) for hemicellulose was higher (P<0.05) in D1 (62.36h) than in D3 (28.00h) and D4 (20.64h). This study demonstrated that low quality forages at 70% of the total diet can be efficiently utilized by sheep when controlled-release urea supplementation is 30% of the feeding regime.

Journal Article↗
PubMed1983

A study of various factors affecting digestion of fish tissue prior to mercury determination.

The effect of temperature on digestion, the acid combination and their quantities, and the time required for digestion of fish tissue were investigated using Hamour (Epinephelus tauvina) body tissue. Concentrations of Hg in fish tissue digested for four hours at 80 +/- 2 degrees C and 95 +/- 2 degrees C were statistically similar and significantly higher than in tissue digested at 60 +/- 2 degrees C. Eight acid combinations were investigated as digestion media and a 1:2 mixture of concentrated HNO3:H2SO4 proved to be the best. A quantity of 15 ml of this digestion media were found to be sufficient to digest approximately two grams of wet fish tissue. The use of 25 ml of digestion media resulted in significantly reduced Hg concentration whereas 10 ml was not sufficient to digest two grams of fish tissue. A digestion period of four to six hours at 80 degrees C was sufficient to oxidize the fish tissue. However, a two hour digestion resulted in reduced Hg values. Mercury determinations made from the samples prepared by the best combination of all the experimental conditions showed a good agreement with those of samples prepared in Teflon Acid Digestion Bombs. This study has pointed the necessity of developing a uniform standard procedure for digesting fish tissue prior to Hg determinations.

Acids↗
PubMed1997

Current perspective on assessing site of digestion in ruminants.

The site of nutrient digestion in the gastrointestinal tract of ruminants affects nutrient availability and the nature of digestive end-products supplied to the host animal. Methods to study site of digestion in vivo provide a tool to obtain data that enhance the ability to interpret or predict animal responses to some feeding practices. Examples are discussed for which site of digestion data provided insights that might not have been evident from other approaches. The use of site of digestion techniques may provide interpretation regarding digestion of N by ruminants different from those derived from measurement of total tract N digestion. Site of digestion measurements have been particularly important in studying effects of heat processing of protein sources and in understanding the nature of digestion of N in high-quality, fresh forages. Moreover, site of digestion techniques have been instrumental in interpreting the influences of supplemental fat sources on ruminal digestion and ruminal biohydrogenation and small intestinal digestion of long-chain fatty acids.

Animals↗
PubMed2001

Effect of cellulose on the digestibility of high starch versus high fat diets in dogs.

The effects of cellulose added in three levels (7, 15 and 20% crude fibre in dry matter) to three different basal diets (a high fat diet, a high starch diet with raw starch and a high starch diet with cooked starch) on apparent digestibility were investigated in eight adult dogs. Cellulose had little effect on the apparent digestibility of fat. In the high fat diet there was no significant decrease, not even at the highest cellulose level (98.3% compared with 98.1% in the basal high fat diet). In the cooked starch diets, fat digestibility decreased from 95.1% in the basal cooked starch diet to 93.8% at the highest cellulose level. In the raw starch diets, digestibility did not decrease with increasing cellulose levels. The apparent digestibility of crude protein was considerably decreased by cellulose in all diets. Starch also decreased protein digestibility and the effects of cellulose and starch appeared to be additive (high fat diet decrease of protein digestibility from 86.7 to 83.5%, cooked starch from 81.6 to 78.6%, raw starch from 79.0 to 70.8%, basal diets to highest cellulose levels, respectively). The apparent digestibility of nitrogen-free extract decreased from 93.9% in the basal cooked starch diet to 84.5% at the highest cellulose level. The figures for the raw starch diets were similar (decrease from 93.4 to 85.9%). Cellulose decreased the apparent digestibility of energy in all diets. This decrease was more marked in the high starch diets (cooked starch decrease from 89.1 to 69.6%, raw starch from 88.9 to 70.2%) than in the high fat diet (decrease from 90.1 to 76.1%). An evaluation of previous data showed that in general fibre has a higher impact on the apparent digestibility of energy in high carbohydrate diets than in low carbohydrate diets. The apparent digestibility of potassium, sodium and chloride was impaired by cellulose. The apparent digestibility of these minerals tended to be lower in the high starch diets, especially in the raw starch diet, and the effect of cellulose was usually more marked in those diets.

Animal Feed↗
PubMed1996

The effect of condensed tannins from heated and unheated cottonseed on the ileal digestibility of amino acids for the growing rat and pig.

The effect of condensed tannins (CT) from heated and unheated cottonseed on the apparent ileal digestibility of amino acids for the growing rat and pig was determined. In Expt 1, twenty-four rats were allocated to four semi-purified diets which contained cottonseed kernel and hulls as the only protein source. Two of the diets contained unheated solvent-extracted cottonseed kernel and hulls, while the remaining two diets contained similar material but which had been heat-treated by autoclaving at 110 degrees for 120 min. In Expt 2, twelve rats and twelve pigs were fed on four semi-purified diets containing commercial cottonseed meal (CSM) as the only protein source. Cr2O3 was added to all diets as an indigestible marker. For each pair of diets in both experiments, PEG was either included or excluded. The effect of CT was assessed by comparing control animals (-PEG; CT acting) with PEG-supplemented animals (+PEG; CT inactivated). Ileal contents from the terminal 150 and 450 mm of ileum were collected at slaughter, 7 h from the start of feeding, for the rats and pigs respectively. Apparent ileal amino acid digestibility for rats fed on the diet containing cottonseed kernel and hulls was significantly depressed by the heat treatment, particularly for lysine and threonine. On average, apparent ileal amino acid digestibility in the diets without PEG was decreased from 0.80 to 0.70 by heat treatment. Dietary cottonseed CT depressed apparent ileal protein digestibility in the pig and in the rat. The addition of PEG to the diets significantly increased the apparent ileal digestibility of N and some amino acids for the pigs and the rats. The mean increase in apparent ileal digestibility due to PEG addition for the fourteen amino acids was 2 percentage units in both species fed on the commercial CSM diets, and 2 or 4 percentage units in rats fed on the unheated or the heated cottonseed kernel and hull diets respectively. The effect of PEG was similar in the heated and unheated cottonseed kernel and hulls for most amino acids, but apparent ileal digestibilities of threonine, tyrosine and lysine were increased more by PEG in heated than in unheated CSM. Apparent ileal N digestibility was lower in the pig than in the rat. For several of the amino acids there were significant animal species differences in apparent ileal digestibility. Studies into the effects of cottonseed CT should be carried out in the target animal species. The commercial CSM had a low apparent ileal amino acid digestibility overall, particularly for the essential amino acids lysine and threonine. It was concluded that effects of heating did not eliminate the reversible reactivity of cottonseed CT on amino acid digestion in rats and pigs but rather appeared to increase it for threonine, tyrosine and lysine in Expt 1, causing large reductions in apparent ileal digestibility of these amino acids.

Amino Acids↗
PubMed2005

Influence of age on the apparent ileal amino acid digestibility of feed ingredients for broiler chickens.

The apparent ileal digestibility of amino acids in 8 feed ingredients was determined using 14-, 28- and 42-d-old male broiler chickens. The ingredients included three cereals (wheat, sorghum and maize), one cereal by-product (mill run), three oilseed meals (canola, cottonseed and soybean meals) and one animal protein meal (meat and bone meal). Dietary crude protein in the assay diets was supplied solely by the test ingredient. All diets contained 20 g/kg acid-insoluble ash as an indigestible marker, and each diet was offered ad libitum in mash form to 5 replicate pens from 11 to 14 d, 25 to 28 d and 39 to 42 d post-hatching. There were 12, 6 and 6 birds per pen for the 14, 28 and 42 d samplings, respectively. The results suggest that the age of broilers significantly influenced the apparent ileal digestibility of amino acids. The effects, however, varied among amino acids and ingredients. In wheat, the digestibility of most amino acids was higher in 14- than in 28- and 42-d-old broilers. In maize, the digestibility coefficients of amino acids were higher at 28 and 42 d than at 14 d. The digestibility coefficients in maize and wheat at 28 and 42 d were similar. The digestibility of amino acids in sorghum at 42 d was higher than those at 28 d, but similar to those at 14 d except for histidine, lysine, serine and glycine, which were significantly higher at 42 d. Digestibility of amino acids in sorghum was similar between 14 and 28 d except for isoleucine, leucine, phenylalanine, glutamic acid and alanine. The digestibility of amino acids in mill run at 42 d was significantly higher than those at 14 and 28 d. There were no differences in digestibility between 14 and 28 d. In general, the digestibility of amino acids in canola meal, soybean meal and, meat and bone meal was higher at 28 and 42 d compared to those at 14 d, and similar between 28 and 42 d of age. In cottonseed meal, age had no effect on the digestibility coefficient of amino acids, except for lysine and arginine, which increased with age. Analysis of the combined results for the 8 feed ingredients showed that, in general, the digestibility coefficients of amino acids increased with advancing age of broiler chickens.

Aging↗
PubMed2005

Variation in precaecal amino acid and energy digestibility between pea (Pisum sativum) cultivars determined using a linear regression approach.

An experiment was conducted to study the variation in the precaecal amino acid (AA) and energy digestibility of 4 cultivars of white-flowering peas (Pisum sativum) for broiler chickens. The 4 cultivars were grown and harvested under the same agronomic and environmental conditions. One basal diet and 8 diets including each pea cultivar at inclusion rates of either 150 or 300 g/kg were used. Peas were included at the expense of starch. Hence, the differences in dietary crude protein and AA concentrations resulted only from the inclusion of peas. Titanium dioxide was included as an indigestible marker. Crude protein concentrations in the complete diets ranged from 164 to 244 g/kg. All diets were fed ad libitum to broiler chickens between 14 and 21 d of age. Seven pens of 12 chickens were allocated to each treatment. Digesta were sampled on a pen basis from the section of the gastrointestinal tract between Meckel's diverticulum and 2 cm anterior to the ileo-caeco-colonic junction. The proportions of crude protein and AAs digested responded linearly to increased intake and the relationships between quantitative intake and digested amounts of AAs were described by multiple linear regressions. The slope determined for each pea cultivar was taken as a measure of AA digestibility without the need for consideration of basal endogenous crude protein and AA secretions. Digestibility of AAs in peas ranged from 0.60 to 0.91. One cultivar had significantly lower AA digestibilities than the other three cultivars. This could be explained neither by the trypsin inhibitor activity nor by tannin levels. The AAs with the highest digestibilities in all cultivars were arginine > glutamic acid > lysine > methionine > phenylalanine > leucine. The AAs with the consistently lowest digestibilities were cystine < threonine. The ranking of the remaining AAs varied slightly between cultivars. The multiple linear regression approach is suitable to study differences in AA digestibilities without the need for consideration of basal endogenous AA losses. Diets containing 300 g/kg of peas had significantly lower energy digestibilities than the pea-free basal diet. The pea cultivar with the lowest AA digestibility caused the lowest energy digestibility at both levels of pea inclusion.

Amino Acids↗
PubMed1999

A comparison of ileal digesta and excreta analysis for the determination of amino acid digestibility in food ingredients for poultry.

1. The apparent ileal and excreta digestibilities of amino acids in 15 samples representing 12 food ingredients were determined using 5-week-old male broiler chickens. The ingredients included 3 samples of cereals (wheat, maize and sorghum), 6 samples of plant protein meals (soyabean meal, cottonseed meal, canola meal and sunflower meal) and 6 samples of animal protein meals (meat meal, meat-and-bone meal, feather meal and fish meal). 2. The test ingredients were incorporated as the sole source of dietary protein in assay diets. Each diet was offered ad libitum to 3 pens (4 birds/pen) from d 35 to d 42 post-hatching. Total collection of excreta was carried out during the last 4 d. All birds were killed on d 42 and the contents of the lower half of the ileum were collected. Apparent ileal and excreta amino acid digestibilities were calculated using acid-insoluble ash as the indigestible marker. 3. The influence of site of measurement was found to vary among food ingredients, among samples within an ingredient and among different amino acids within an ingredient. Ileal amino acid digestibility values were similar in some ingredients, but significantly lower or higher in others than the corresponding excreta values. 4. Average ileal and excreta amino acid digestibilities in sorghum and maize were similar, but significant differences were observed for individual amino acids. In contrast, ileal amino acid digestibility values were higher than the corresponding excreta digestibility values in wheat. 5. The average ileal and excreta digestibilites of amino acids in the 3 soyabean meal samples were similar although small, but significant differences were noted for individual amino acids. Site of measurement had no effect on the digestibility of amino acids in canola meal. Digestibilities of valine, isoleucine, phenylalanine, histidine, glutamic acid, alanine and tyrosine in sunflower meal and those of valine, methionine, isoleucine, leucine, lysine, glutamic acid and alanine in cottonseed meal were lower by excreta analysis. 6. Digestibilities in animal protein meals, with the exception of blood meal and fish meal, were consistently higher by excreta analysis. Ileal-excreta differences in individual amino acid digestibilities were more evident in feather meal, meat meal and meat-and-bone meal. 7. Threonine and valine were the indispensable amino acids that were more frequently influenced by the site of measurement. Of the dispensable amino acids, aspartic acid, serine, glutamic acid and alanine were the most affected. 8. Differences determined between ileal and excreta digestibilities in the present study clearly demonstrate that amino acid metabolism by hindgut microflora in chickens may be substantial and that digestibilities measured in the terminal ileum are more accurate measures of amino acid availability than those measured in the excreta.

Amino Acids↗
PubMed1975

[Determination of the true digestibility of amino acids in variously prepared potatoes in growing pigs. 1. Freshly steamed potatoes and silage made from steamed potatoes].

Freshly Steamed Potatoes of 6 varieties were investigated. With 3 varieties the true N and amino acid digestibilities were found to be in good agreement, while great variations were found in potatoes of the other 3 varities so that it appears to be a matter of some doubt whether the true amino acid digestibility could, in the case of potatoes, be reliably derived from data on the true N digestibility. Differences in the true amino acid digestibility data were found between the varities of potatoes grown in different years. A positive trend was found to exist in the relationship between the absolute amino acid content and true digestibility. The true lysine digestibility of freshly steamed potatoes varies between 76-90%, the average percentage being about 80%. The true methionine digestibility was found to vary within still wider limits, although the average percentages was also about 80%. Values established for the true cystine digestibility ranged from 80% to 90%. The true amino acid digestibility data did not provide a sufficiently reliable basis for making conclusions as to the existence of a limiting amino acid. Both the lowest value for true digestibility and the greatest variations were found in the case of the amino acids alanine, tyrosine, and methionine. It was observed that silage made from steamed potatoes had a lower true digestibility of N than steamed fresh potatoes although the value for true amino acid digestibility were the same, with the exception of methionine and cystine.

Amino Acids↗