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Sonographically guided hydrostatic reduction of childhood intussusception using Hartmann's solution.

OBJECTIVE: Currently, the standard methods for therapeutic reduction of intussusception in children involve considerable ionizing radiation. This study tested the effectiveness of sonographically guided hydrostatic reduction of intussusception using Hartmann's solution, a fluid with near-physiologic composition. SUBJECTS AND METHODS: Between March 1, 1994, and January 31, 1996, all children clinically suspected of having intussusception were evaluated by sonography. Those with positive findings on sonography were entered into the study and underwent confirmatory sonographically guided meglumine diatrizoate enema. During continuous sonographic monitoring, we used Hartmann's solution for attempted reduction of intussusception. Criteria for successful reduction were disappearance of the intussusceptum and passage of fluid through the ileocecal valve. Another sonographically guided meglumine diatrizoate enema was used to confirm successful reduction. RESULTS: We detected 25 consecutive intussusceptions in 22 patients. The patients were 12 girls and 10 boys, with a mean age of 14 months (range, 1-72 months). Sonograms revealed in all patients doughnut or pseudokidney signs or both. The sites of intussusception were the transverse colon (17 of 25), hepatic flexure (4 of 25), ascending colon (2 of 25), splenic flexure (1 of 25), and descending colon (1 of 25). Other findings were dilated fluid-filled small bowel (11 of 25) and free intraperitoneal fluid (9 of 25). The success rate of our sonographically guided attempts at hydrostatic reduction was 76% (19 of 25). Success was proven by meglumine diatrizoate enema in all 19 patients. The mean time of the reduction procedure was 18 min (range, 2-45 min). No complications occurred. All six patients in whom hydrostatic reduction was unsuccessful underwent surgery. Five of these patients had ileoileocolic intussusceptions. On sonography, when surrounded by fluid, ileoileocolic intussusceptions had a typically complex, fronded appearance. The remaining patient in whom hydrostatic reduction was unsuccessful had ileocolic intussusception. Of six ileoileocolic intussusceptions, one was hydrostatically reduced and a second was converted into an ileoileal intussusception before requiring surgery. The other four intussusceptions were surgically treated. CONCLUSION: Our data suggest that sonographically guided hydrostatic reduction with Hartmann's solution can be used to treat ileocolic intussusception and to diagnose ileoileocolic intussusception.

Catheterization↗

The effects of water-soluble contrast media on luminal distension and blood flow in closed loops of small bowel in minipigs.

The effects of iohexol, sodium diatrizoate, and physiologic saline on intestinal distension and circulation were observed for 8 h in nine minipigs with closed-loop obstruction of the small bowel. The two contrast media led to an elevation of intraluminal pressures when initially instilled at pressures above 35 mm Hg. These elevated pressures were not high enough to cause rupture of the bowel wall. Both contrast media caused severe mucosal ischaemia as judged from histologic sections, loops containing sodium diatrizoate more so than iohexol. The blood circulation of the bowel wall, examined by laser Doppler flowmetry, was after 6 to 8 h reduced to about 10% of the values of non-obstructed bowel at intraluminal pressures of about 70 mm Hg in the loops with iohexol and sodium diatrizoate. The correlation to osmolality was obvious when compared with concurrent observations in the loops with physiologic saline. In the bowel loops filled with physiologic saline the pressure fell to 5 mm Hg after 8 h, regaining approximately one-third of pre-instillation levels of blood flow. On microscopy these bowel loops had a nearly normal mucosa.

Animals↗

Experimental pancreatography: a comparison of three contrast media.

Twenty-five piglets were subjected to experimental transduodenal pancreatography, using three contrast media (CM), namely sodium meglumine diatrizoate (Urografin), meglumine ioxaglate (Hexabrix), and iohexol (Omnipaque), all diluted to a concentration of 300 mg/ml of iodine. The emptying of CM from pancreatic ducts was studied by repeated roentgenograms, and pancreatic irritation by serum amylase and phospholipase A2 measurements. On the 3rd day after pancreatography the glands were removed for histologic examination of tissue damage. Hyperamylasaemia was seen in 53% of the animals 12 to 15 h after examination, to equal extents in each group. Minimal acinar destruction was seen on histologic examination in all cases. The changes were least when diatrizoate was used. The difference from the changes seen in the ioxaglate group was significant (p less than 0.05). Emptying of CM from the ducts was highly significantly (p less than 0.001) faster in the diatrizoate group than in the other groups. Rapid emptying may be an advantage in clinical endoscopic retrograde pancreaticocholangiography.

Animals↗

[Activation of the complement system and cytokine production by radiographic contrast media in vascular endothelial cells in vitro].

The direct effect of four different radiographic contrast media (RCM) on the release of C3a and C5a and the production of IL-1 alpha and TNF-alpha from vascular endothelial cells was examined in vitro. The test RCM were as follows: diatrizoate (ionic monomer), iopamidol (nonionic monomer), ioxaglate (ionic dimer), and iotrolan (nonionic dimer). These were added to serum-free medium and adjusted to a final concentration of 1% (2.8 mg Iodine/ml). Human microvascular endothelial cells were stimulated by serum-free medium containing the test RCM for eight hours. After incubation, the media were aspirated and assayed for the concentrations of C3a, C5a, IL-1 alpha and TNF-alpha. Finally, the cells were harvested by trypsin, and their viability was determined by the dye-exclusion method. Diatrizoate and iotrolan had higher C3a release than the control (p < 0.05). No increase in C5a, IL-1 alpha or TNF-alpha levels was observed with any of the tested RCM, and there was no significant difference in cell viability with any of the tested RCM. The results of this study suggest that diatrizoate and iotrolan activated the complement system through the alternative pathway by directly stimulating vascular endothelial cells. These observations suggest that a direct effect of RCM on vascular endothelium might play a role in the pathogenesis of local drug eruptions due to RCM.

Cells, Cultured↗

Differential effects of radiocontrast agents on human umbilical vein endothelial cells: cytotoxicity and modulators of thrombogenicity.

UNLABELLED: The endothelium plays a central role in the regulation of blood flow and coagulation. The impact of radiocontrast agents on endothelial cells is therefore potentially clinically important, particularly in percutaneous interventions for acute coronary thrombosis. The effects of radiocontrast agents on endothelial cell viability and determinants of thrombogenicity were studied in human umbilical vein endothelial cells (HUVECs) in vitro. Intercellular tight junctions were assessed using immunofluorescence microscopy and measurement of the transmonolayer electrical resistance (TMR). The concentrations of endothelin-1 (E), von Willebrand factor (vWF), plasminogen activator inhibitor-1 (PAI-1) and thrombomodulin (T) were measured in the cell culture media. The ionic, high osmolal radiocontrast agent diatrizoate induced concentration-dependent cell death and an opening of tight junctions with the attendant abolition of the TMR. The concentration of E decreased, vWF increased in the cell culture media, the concentration of PAI-1 and T was not significantly changed by diatrizoate. Radiocontrast agents with reduced osmolality (ioxaglate: ionic; iopamidol: non-ionic) induced an increase in PAI-1 and vWF, but E and T were not significantly changed. CONCLUSIONS: Radiocontrast agents have differential effects on endothelial cells in vitro including the secretion of modulators of thrombogenesis. The effects are most pronounced in the markedly hyperosmolal compound diatrizoate suggesting a contributory role of hypertonicity. Ioxaglate and iopamidol both increased the prothrombotic factors vWF and PAI-1 to the same degree indicating a similar risk of thrombogenicity between low-osmolal ionic and non-ionic radiocontrast agents in this in vitro model.

Blood Coagulation↗

[Tolerance to standard and non-ionic contrast media in patients with ischemic heart disease and stable angina pectoris during coronary angiography].

Angiography of the coronary arteries with iohexol was carried out in 45 coronary patients with stable angina pectoris and that with diatrizoate in another group of 45 similar patients. Iohexol was tolerated better that diatrizoate. Physiological and hemodynamic side effects of iohexol were less marked than those of diatrizoate.

Adult↗

[Study of the marker for a neuronavigation system].

UNLABELLED: With different ratios, Omnipague mixed with cod liver oil and Meglumine Diatrizoate mixed with cod liver oil are compared with the stardard marker under the scanning of CT and MR in order to find the better ratio and materials for the marker. The experimental results show that the performances of both Omnipague mixed with cod liver oil and Meglumine Diatrizoate mixed with cod liver oil are better than the stardard marker. CONCLUSION: Omnipague mixed with cod liver oil and Meglumine Diatrizoate mixed with cod liver oil can be used in making marker for neuronavigation system.

Biomarkers↗

Effect of an iso-osmolar contrast medium on pulmonary arterial pressure at pulmonary angiography.

A clinical comparison of the effects on pulmonary arterial pressure induced by contrast media with various osmolalities, iohexol 140 mg I/ml (300 mosm/kg H2O), iohexol 300 mg I/ml (690 mosm/kg H2O), and diatrizoate 292 mg I/ml (1480 mosm/kg H2O) following selective pulmonary angiography was made in 12 patients with normal pulmonary arterial pressure. A double-blind crossover study was performed and the contrast media were administered in random order. The pulmonary arterial pressure was recorded continuously before, during, and for 3 min after the injection. The effect of iohexol 140 on the pulmonary arterial pressure was significantly less marked than that of diatrizoate 292, whereas no statistical significance was shown between iohexol 140 and iohexol 300. These results indicate that iso-osmolar contrast medium (iohexol 140), as well as iohexol 300, would be better tolerated than diatrizoate 292, and is therefore a safer contrast medium for selective pulmonary angiography.

Adult↗

Nephrotoxicity of high and low osmolar contrast media: case control studies following digital subtraction angiography in potential risk patients.

The urinary excretion of kidney-specific marker proteins before and 120 hours after intravenous injection of either high- or low-osmolar contrast media (CM; diatrizoate, iopamidol 370) was monitored in patients after digital vascular imaging. Inclusion criteria for the randomized clinical study in a total of 40 patients (15 women, 25 men; mean age, 64.5 years) were at least 50 years of age or diabetes mellitus with normal creatinine concentration in serum. Compared with the control period, the elimination of tubular indicator enzymes alanine aminopeptidase, gamma-glutamyltranspeptidase, alkaline phosphatase, as well as of glomerular localized angiotensinase A was significantly higher in all patients after injection of the CM. The most significant differences were observed after 48 hours. In contrast, lysosomal N-acetyl-beta-D-glucosaminidase activity in urine specimens reacted less clearly and appears to be a less sensitive parameter in assessing CM nephrotoxicity. Elimination of brush border as well as of glomerular marker proteins was significantly lower after intravenous injection of low-osmolar CM iopamidol 370 (832 mOsm/kg) than after meglumine diatrizoate 76 (2100 mOsm/kg). In all 40 patients a significant decrease in creatinine clearance was observed; however, patients receiving diatrizoate had a significant decrease in creatinine clearance (period 0 versus 24 to 48 hours after CM), whereas patients after administration of iopamidol had not. No difference was found between creatinine clearance after 48 hours of CM injection within both groups of CM. Due to noninvasive parameters of kidney damage nonionic, low-osmolar CM are less nephrotoxic in potential risk patients, and should be preferred to conventional CM.

Aged↗

Urinary protein excretion following intravenously administered ionic and non-ionic contrast media in man.

Urinary protein excretion following intravenous administration of the radiographic contrast media (CM) diatrizoate (ionic) and iopromide (non-ionic) was examined in 20 patients with normal renal function. Neither of the two CM had any effect on the 24-h urinary excretion of albumin (a marker of glomerular proteinuria). The 24-h urinary excretion of the retinol-binding protein (a marker of low molecular weight or tubular proteinuria) and the folate binding protein, a protein localized in the brush-border membranes of the proximal tubular cells, showed a statistically significant transient increase the day after diatrizoate injection, whereas no increase was observed after iopromide. Thus, only a minimal and temporary disturbance of the renal proximal tubular function was observed after diatrizoate injection in patients with normal renal function.

Adult↗

Pain in peripheral arteriography: an assessment of conventional versus ionic and non-ionic low-osmolality contrast agents.

Two low-osmolality contrast agents, ioxaglate meglumine/sodium and iohexol were compared with diatrizoate meglumine/sodium in a controlled double blind study of 126 patients undergoing arteriography for peripheral vascular disease to determine which caused the least pain. Discomfort was assessed by means of a visual analog scale rating pain from 0 to 100. Average values for pain were 39 +/- 27 for diatrizoate, 14 +/- 15 for ioxaglate and 21 +/- 22 for iohexol. We found that both low-osmolality agents caused significantly less pain in peripheral arteriography than the traditional agent. The p values were p less than 0.0005 for ioxaglate and p less than 0.005 for iohexol versus diatrizoate. In addition, ioxaglate was found to cause significantly less pain than iohexol (p less than 0.05) in this patient group.

Adult↗

Effect of contrast media on beta-endorphin secretion. An in vitro study.

The central nervous system may be highly susceptible to the toxic effects of contrast media (CM). Previous experiments demonstrated that vasopressin is released after the intravenous administration of CM. The present study examined the response of the opiocortin system to CM. Neurons of the rat basal hypothalamus, dispersed and attached to Cytodex-3 beads, were perfused with sodium diatrizoate, metrizamide or iohexol (3 mg iodine/ml). The effluent was collected, and the beta-endorphin (B-E) content was measured by a radioimmunoassay technique. Results, normalized to the internal positive control, were compared with release from normal saline (negative control) by analysis of variance. Diatrizoate and metrizamide caused significant release of B-E (p less than 0.03). Iohexol did not stimulate release of B-E. These results suggest that diatrizoate and metrizamide, but not iohexol, can stimulate the release of hormones from hypothalamic neurons. The phenomenon may play a role in some reactions to intravascular CM administration since these neurons are not protected by a blood-brain barrier.

Animals↗

Mechanisms of decreased cerebrospinal fluid production by radiographic contrast media: role of adenylate cyclase activation.

The i.v. administration of radiographic contrast media decreases cerebrospinal fluid (CSF) production as measured by negative pressure collection from the lateral ventricles of anesthetized dogs. Evidence suggests that adrenergic-mediated adenylate cyclase (AC) activity controls CSF production. AC activity was measured in membrane fractions of bovine and canine choroid plexus and rat heart and lung in the presence of various concentrations of the contrast agent sodium diatrizoate. A concentration-dependent inhibition of isoproterenol-stimulated AC activity by the contrast agent was observed in vitro. Specific binding of [3H]dihydroalprenolol to membrane fractions of bovine choroid plexus was also inhibited by sodium diatrizoate. An indication that the inhibition of choroidal AC activity by contrast media is significant in vivo was obtained by measuring CSF production in dogs. The inhibition of CSF production by sodium diatrizoate was reduced 50% when the contrast agent was administered during an i.v. infusion of isoproterenol (100 ng/kg/min). These results indicate that the mechanism of decreased CSF production by contrast media may involve inhibition of beta adrenergic-stimulated AC activity.

Adenylyl Cyclases↗

Hypernickelemia following coronary arteriography, caused by nickel in the radiographic contrast medium.

Meglumine diatrizoate ("Renografin-76", a radiographic contrast medium) contains sufficient nickel to cause hypernickelemia in patients after coronary arteriography. Nickel analyses by electrothermal atomic absorption spectrophotometry showed that nine lots of "Renografin-76" (760 g of meglumine diatrizoate per L) contained 144 +/- 44 micrograms Ni per L. Serum Ni concentrations became elevated in 11 patients after coronary arteriography (Ni dose = 19 +/- 4 micrograms per patient); peak Ni concentrations (increment = 1.8 +/- 0.4 micrograms Ni per L) occurred 0.25 or 0.5 h post-injection. Serum Ni concentrations diminished at 2 and 4 h post-injection and returned to base-line values at 24 h. The half-time (T1/2) for reduction of serum Ni concentrations averaged 1.5 h. Analysis of urine specimens from two patients showed that most of the Ni dose was excreted in urine within 24 hours. After iv administration of meglumine diatrizoate to rabbits (0.5 or 1.0 micrograms Ni per kg body wt), T1/2 values for elimination of Ni from the serum volume averaged 1.2 h, compared to T1/2 values of 5.7 and 7.4 h, respectively, when Ni was administered iv in NiCl2 or albumin solutions. Since "Renografin-76" contains edetate disodium (0.4 g per L), Ni is probably present as a Ni-EDTA complex, accounting for the rapid elimination of Ni following iv administration of the contrast medium to patients and rabbits. To reduce possible hazards of allergic or cardiovascular reactions to nickel, the authors recommend that Ni concentrations in radiographic contrast media should not exceed 10 micrograms per L.

Adult↗

Direct cardiac effects of an ionic and a non-ionic contrast medium in dogs.

Direct cardiac effects of ionic diatrizoate (Uromiro) and non-ionic iopamidol (Iopamiro) were investigated in "in situ" heart-lung preparation of 19 vagotomized dogs. Diatrizoate was found to induce considerably greater alteration in plasma osmolality and subsequent dehydration of the myocardium compared with iopamidol. Myocardial dehydration resulted in a decrease of left ventricular compliance and in that of cardiac performance. Diatrizoate was shown to influence the myocardium not only by its hyperosmolarity but also by a direct action. Heart rate was reduced by both contrast media.

Animals↗

Renal excretion mechanism of a nonionic contrast agent.

The renal excretion mechanism of a nonionic experimental contrast agent, C-29, was investigated in rabbits in comparison with the ionic contrast agent sodium diatrizoate under conditions of free urine flow and ureteral stasis. The two contrast agents were injected as a mixture of 125I-C-29 and 131I-sodium diatrizoate, in doses of 25 and 250 mg I/kg. The results indicate that, during the period relevant for urographic examinations, C-29 may have a renal clearance as low as 0.7 times that of sodium diatrizoate under conditions of both free urine flow and ureteral stasis. The biologic half-life of C-29 is 41-50 min.

Animals↗

The effect of ionic and nonionic contrast media on the metabolism of prostaglandin E2 in rat lungs.

Pulmonary inactivation of prostaglandin E2 (PGE2) was investigated in isolated perfused rat lungs during infusion of ionic and nonionic radiographic contrast media (RCM). When 100 nmol of [14C]-PGE2 was infused slowly into the pulmonary circulation, the ionic ioglycamate decreased the metabolism of PGE2, but other ionic (diatrizoate and ioxaglate) and nonionic RCM (iopamidol and metrizamide) had no significant effect. When a smaller amount of [14C]-PGE2 (10 nmol) was injected as a bolus the metabolism of PGE2 was decreased also by diatrizoate and ioxaglate, but not by iopamidol. After a similar bolus injection of 10 nmol of [14C]-PGE2, the efflux of radioactivity from the lungs was increased by diatrizoate, ioglycamate and ioxaglate but remained unchanged by iopamidol and metrizamide. The RCM infusion did not change the perfusion pressure. The present study indicates that ionic RCM decrease the inactivation of PGE2 in rat lungs and thus possibly increase the circulating level of this prostaglandin.

Animals↗

Neurotoxic effects of water-soluble contrast agents on rat hippocampus: extracellular recordings.

Synaptically evoked extracellular field potentials were obtained from the pyramidal cell layer of the Cal field of rat hippocampus slices. Synaptic activity was evoked by Schaffer's collateral stimulation. The effects of the water-soluble contrast agents Na-diatrizoate and metrizamide, as well as control solutions of NaCl and sucrose, were studied. Test solutions were 300-345 mOsm and had iodine concentrations of 3-30 mg I/ml. The effects of test solutions are apparent within 10 minutes and reversible within 30-45 minutes. Control recordings have a small positive potential, followed by a large negative spike and a final slow positive wave. Contrast agents have two effects. The first is an epileptogenic property characterized by repetitive negative spikes arising from an extended slow positive field. The second effect is a depression characterized by a reduction in the amplitude of the synaptically evoked fields, particularly the negative spikes. Na-diatrizoate predominately produces the first effect, while metrizamide principally produces the second. Hyperosmolar solutions only produce depression. These results correlate well with the clinical setting where Na-diatrizoate is a potent convulsant, while metrizamide produces symptoms compatible with neuronal depression. These results indicate that contrast agent seizure activity is not due to hyperosmolarity but to a more direct chemical effect. The depression, however, may be related to a hyperosmolar effect.

Animals↗