Search PubMed⌕ Search

SEARCH · Search PubMed

Results for “DENTAL PULP”

Search indexed PubMed citations on genomics, clinical trials, systematic reviews and public health. Explore titles, authors and supplied subject terms, then open the PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 505 records · Page 28Linked to original sources
PubMed2004

Blood-flow change and recovery of sensibility in the maxillary dental pulp during and after maxillary distraction: a pilot study.

OBJECTIVE: To examine the change in blood flow and recovery of sensibility in the maxillary dental pulp during and after maxillary distraction. STUDY DESIGN: The subjects included 5 patients undergoing high Le Fort I osteotomy and maxillary distraction (D-group) and a reference group of 14 patients who underwent a common single-segment Le Fort I osteotomy, 1-stage maxillary advancement, and mandibular setback surgery (C-group). Eleven (D-group) and 54 (C-group) maxillary incisors were assessed preoperatively and at 1-7 days, 14 days, and 3 months postoperatively. Pulpal blood flow (PBF) was measured by laser Doppler flowmetry, and pulpal sensibility (PS) was investigated by electrodiagnostics. RESULTS: From postoperative days 1 to 5 (the latency period), the PBF tended to be higher in the D-group than in the C-group. From day 6 to 3 months postoperatively (during and after maxillary distraction), the PBF values of the 2 groups were similar. The PS remained negative up to 14 days postoperatively in both groups. However, at 3 months after the operation, a higher proportion (90.9%) of teeth in the D-group was positive for PS. CONCLUSION: These results suggest that maxillary distraction is a favorable technique for maintenance of PBF and recovery of PS in the maxillary teeth after surgery.

Adolescent↗
PubMed2007

Stimulatory effects of hydroxyl radical generation by Ga-Al-As laser irradiation on mineralization ability of human dental pulp cells.

The present study was conducted to investigate the effects of Ga-Al-As laser irradiation on the mineralization ability of human dental pulp (HDP) cells. HDP cells in vitro were irradiated once with a Ga-AL-As laser at 0.5 W for 500 s and at 1.0 W for 500 s in order to investigate free radicals as one mechanism for transmission of laser photochemical energy to cells. Production of the hydroxyl radical (*OH) was measured using the ESR spin-trapping method and was found to be increased by laser irradiation. The DMPO-OH was not detected in the presence of dimethyl sulfoxide (DMSO), a *OH scavenger. The formation of calcification nodule was also investigated by von Kossa staining. The number of calcified nodules was increased by 1.0 W-laser irradiation. Alkaline phosphatase (ALP) activity was higher in the 1.0 W-laser irradiation group. Expression of mRNAs for heat shock protein 27, bone morphogenetic proteins (BMPs) and ALP were greater in the 1.0 W-laser irradiation group. Expression of BMPs in the conditioned medium was also higher in the 1.0 W-laser irradiation group. In particular, DMSO decreased the number of calcified nodule produced by 1.0 W-laser irradiation. These results supposed that the mineralization of HDP cells is stimulated by laser irradiation, and that *OH generated by laser irradiation is a trigger for promotion of HDP cell mineralization.

Alkaline Phosphatase↗
PubMed2004

Molecular detection of Bartonella spp. in the dental pulp of stray cats buried for a year.

Bartonella henselae causes chronic bacteremia in cats. To test if B. henselae DNA can be recovered from the dental pulp of cats buried a year previously, we used PCR with primers for a sequence of the conserved groEL to test 104 teeth from 11 cats. Seven of the cats were found positive; canine teeth were more frequently positive than molar teeth. Where PCR sequences could be determined, they were identical to those of B. henselae Marseille (four cats), B. henselae Houston (one cat) or similar to those of B. grahamii (one cat). Our study indicates that dental pulp from the teeth of cats, especially the canine teeth, may be used for the PCR detection of Bartonella in animals buried for a year.

Animals↗
PubMed1992

[Micromorphological studies of the blood vessels of the dental pulp of small ruminants].

The blood vascular supply of the dental pulp of 15 domestic sheep of varying age and sex was investigated using light and electron microscopy. The pulp vascular system is showing arterioles with a media of varying thickness and with endothelial cells of variable structure. In the transitional zone between crown and root pulp, the arterioles are taking a typical S- or U-shaped course. The capillary network of the crown pulp is denser and more regular than that in the root pulp. In addition to the capillaries lined by a continuous endothelial tube, there may be others with a typically fenestrated endothelial lining. Specialised vascular structures of the peripheral blood vessels, especially arterio-venous anastomoses as described in man, have not been observed in the sheep.

Animals↗
PubMed2000

Cadmium induces expression of metallothionein in rat dental pulp.

Metallothionein (MT) is a metalloprotein with high affinity for certain heavy metals. In the present study, the ability of cadmium (Cd) to induce the synthesis of MT in dental pulp was investigated. Rats were injected subcutaneously with CdCl2 (1.5 mg Cd/kg) daily for 7 days. Expression of the MT gene was demonstrated by reverse transcription polymerase chain-reaction techniques. The results indicate that Cd induced MT in dental pulp of rat incisor and this may be involved in the cellular defense mechanism against Cd toxicity.

Animals↗
PubMed1983

Membrane junctions in the subodontoblastic region. A freeze-fracture study of the human dental pulp.

The morphology of the subodontoblastic region in the human dental pulp was analysed using the freeze-fracturing technique. Multiple areas of focal intercellular junctions were found between subodontoblastic fibroblasts (or Höhl's cells). The connections were of the gap junction type. Similar junctions were observed between subodontoblastic cells and small-calibre (0.10-0.20 microns) fibres, presumably nerves. Tight junctions were not observed.

Adolescent↗
PubMed1991

Optical determination of dental pulp vitality.

In this preliminary study, we explored the feasibility of employing photoplethysmography and pulse oximetry to assess the status of the blood circulation in the dental pulp. A simple photometer that measures diffuse light transmission at 575 nm was built to record tooth plethysmograms, and the ability to distinguish vital from surgically devitalized teeth of a dog using plethysmography was demonstrated. As an extension of the photoplethysmographic technique, red-infrared pulse oximetry applied to the measurement of the oxygen saturation (SO2) of blood in the pulp was also examined using an in vitro test setup. Results suggest that the measurement of relative SO2 changes is feasible, but standard dual-wavelength pulse oximetry does not enable determination of SO2 independent of tooth geometry and sensor placement.

Adult↗
PubMed1982

Stimulation of prostaglandin E2 biosynthesis in rat dental pulp explants in vitro by 5-hydroxytryptamine.

When excised rat dental pulp tissue was incubated in Krebs solution, prostaglandin E2 (PGE2) was released into the medium. The release was completely blocked by the PG synthetase inhibitor, indomethacin (0.1 mM). Exogenous arachidonic acid (0.01 mM), a substrate of PG synthetase, increased the amount of PGE2 production and the endogenous PGE2 biosynthesis was strongly stimulated by 5-hydroxytryptamine (5-HT) at concentrations higher than 0.01 mM. Bradykinin (0.1 mM) and histamine (0.1 mM) had no effect.

Animals↗
PubMed1979

Electrophysiological properties of human dental pulp cells.

Resting membrane potentials and electrotonic potentials of human dental pulp cells (peripheral and inner cells) were recorded from intracellular measurements. The results show a high resting membrane potential (- 80 mV) for peripheral cells and a low resting membrane potential (- 30 mV) for inner cells. The peripheral cells present rectification properties (outward going rectification and delayed rectification) and a negative post potential at the end of the depolarizing current. These properties are similar to those found in some receptor cells. On the basis of their morphological characteristics, these peripheral cells are compared to odontoblasts in their early stage of development.

Adolescent↗
PubMed2001

Immunohistochemical evidence for ATP receptors in human dental pulp.

Evidence is accumulating which supports a role for ATP in the initiation of pain by acting on P2X receptors, in particular P2X3, expressed on nociceptive afferent nerve terminals. To investigate whether this receptor plays a role in dental pain, we studied the presence and distribution of P2X3 receptors in human dental pulp, and their co-localization with other neural markers. Pulps were removed from extracted third molars and immunohistochemically stained with an antibody against P2X3 receptors. P2X3 immunoreactive (-ir) nerve fibers were detected in the main body of the pulp, in the sub-odontoblastic plexus of Raschkow, and within the odontoblastic area. Co-localization of the P2X3-ir neurons with neurofilament protein (NF) showed that the majority of the fibers were positive for both NF and P2X3. Double labeling with isolectin B4 (IB4) showed that all P2X3-ir neurons also bind IB4. We conclude that P2X3 receptors are present on both myelinated and unmyelinated nerve fibers in human dental pulp and may play a role in dental pain mechanisms.

Adenosine Triphosphate↗
PubMed1997

Stimulation of interleukin-1 beta production of human dental pulp cells by Porphyromonas endodontalis lipopolysaccharide.

IL-1 beta is synthesized as an inactive precursor, which is subsequently processed by IL-1 beta converting enzyme (ICE) and found extracellularly as a mature biologically active polypeptide. Also, IL-1 beta has been detected in necrotic and inflamed dental pulp. We examined the IL-1 beta production in human dental pulp (HDP) cells treated with lipopolysaccharide (LPS) from Porphyromonas endodontalis (P. e.) isolated from root canals and radicular cyst fluids. We demonstrated that P. e. LPS stimulated IL-1 beta release from HDP cells in a time- and dose-dependent manner. However, ICE activity was not increased by P. e. LPS. Northern blot hybridization analysis revealed that the IL-1 beta mRNA level in HDP cells was increased by P. e. LPS. These results suggest that stimulation of IL-1 beta release from HDP cells by P. e. LPS may have an important role in the progression of inflammation in pulpal and periapical disease.

Caspase 1↗
PubMed1994

Histological observations of hard tissue barrier formation in amputated dental pulp capped with alpha-tricalcium phosphate containing calcium hydroxide.

This study was performed to evaluate the pulpal response to alpha-tricalcium phosphate (alpha TCP) containing calcium hydroxide (Ca(OH)2). The dental pulps of monkeys were amputated and dressed with four agents: alpha TCP, alpha TCP containing 1% Ca(OH)2, alpha TCP containing 5% Ca(OH)2, and Ca(OH)2 being used as a control. The pulpal responses were histologically evaluated after 4 and 8 weeks. The pulp tissue treated with alpha TCP proliferated above the level of the original wound surface, and a thin layer of hard tissue barrier was formed directly against the capping agent. The barrier demonstrated atubular matrix lined with flattened or cuboidal cells, but occasionally appeared irregular in form. Ca(OH)2 dressing resulted in destruction of pulp tissue, with a thick hard tissue barrier being formed below the level of the exposure site. The barrier consisted coronally of osteodentin and pulpally of tubular dentin lined with odontoblast-like cells. By contrast, 1% Ca(OH)2 added to alpha TCP produced a slight proliferation of pulp tissue. An atubular matrix barrier, pulpally lined with cuboidal cells, formed above the exposure site. It was later followed by the formation of tubular matrix lined with columnar cells. Teeth treated with 5% Ca(OH)2 showed a thin necrotic layer and a thick barrier formation. The barrier was composed of tubular dentin-like tissue lined with odontoblast-like cells. It would appear that alpha TCP containing a small amount of Ca(OH)2 may be clinically useful as a capping agent, as it induced consistent hard tissue formation, without excessive destruction of underlying pulp tissue.

Animals↗