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Results for “pathogenicity classification”

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At least 487 records · Page 27Linked to original sources
PubMed1997

Use of reverse transcriptase polymerase chain reaction for detection of vaccine contamination by avian leukosis virus.

A reverse transcriptase polymerase chain reaction (RT-PCR) for avian leukosis virus (ALV) was developed for the detection of contamination of vaccines produced in embryonated eggs and cell cultures derived from chicken. ALV is highly pathogenic and induces a wide spectrum of disease in infected animals. ALV can be divided into five subgroups (A-E). The envelope glycoprotein (env gp85) is the main antigen determinant and responsible for subgroup classification. Viral RNA of all subgroups (A-E) was isolated and amplified using three sets of primers. Subsequently, restriction endonuclease analysis confirmed the product identity and discriminated between subgroups. In specific pathogen free (SPF) eggs experimentally inoculated with ALV, viral RNA was found in allantoic fluids, as well as in vaccines spiked with different subgroups of ALV. No adventitious virus was detected in commercially available preparations. This system provides a rapid and specific in vitro method for the detection of ALV RNA as an extraneous agent and may be applied for quality control of avian vaccines.

Animals↗
PubMed1985

The classification and identification of the anaerobic gram-positive cocci.

The Approved Lists of Bacterial Names (1980) includes the following Peptococcus and Peptostreptococcus species pathogenic for man: Peptococcus magnus, P. prevotii, P. asaccharolyticus, Peptostreptococcus anaerobius, P. micros and P. productus. A presumptive identification of these species can be made on the basis of cellular morphology, susceptibility to novobiocin, characterization of metabolic end products, fermentation of glucose and lactose, and production of indol. Capnophilic and microaerophilic streptococci are characterized by their production of lactic acid from glucose.

Bacteriological Techniques↗
PubMed2006

A classification of tasks for the systematic study of immune response using functional genomics data.

A full understanding of the immune system and its responses to infection by different pathogens is important for the development of anti-parasitic vaccines. A growing number of large-scale experimental techniques, such as microarrays, are being used to gain a better understanding of the immune system. To analyse the data generated by these experiments, methods such as clustering are widely used. However, individual applications of these methods tend to analyse the experimental data without taking publicly available biological and immunological knowledge into account systematically and in an unbiased manner. To make best use of the experimental investment, to benefit from existing evidence, and to support the findings in the experimental data, available biological information should be included in the analysis in a systematic manner. In this review we present a classification of tasks that shows how experimental data produced by studies of the immune system can be placed in a broader biological context. Taking into account available evidence, the classification can be used to identify different ways of analysing the experimental data systematically. We have used the classification to identify alternative ways of analysing microarray data, and illustrate its application using studies of immune responses in mice to infection with the intestinal nematode parasites Trichuris muris and Heligmosomoides polygyrus.

Allergy and Immunology↗
PubMed1993

Aetiology and treatment of community-acquired pneumonia in adults: an historical perspective.

Community-acquired pneumonia is common. Most disease is mild but mortality among hospitalized patients is 5-20%. The most common aetiological pathogens are Streptococcus pneumoniae, Haemophilus influenzae, and the 'atypical' organisms, Mycoplasma pneumoniae, Legionella pneumophila and Chlamydia pneumoniae. Less common pathogens account for 10-30% of cases and the aetiology cannot be determined in one-third to one-half of cases. Classification by aetiology and initiation of specific antimicrobial therapy are difficult and treatment is often initiated empirically. Ampicillin (or amoxycillin) or erythromycin are inexpensive and effective for most patients, but their use in combination, the addition of a beta-lactamase inhibitor (e.g. amoxycillin/clavulanate) or the substitution of an expanded spectrum cephalosporin (e.g. cefuroxime) should be considered for patients with more serious illnesses or pathogens likely to be drug-resistant. Fluoroquinolones such as ciprofloxacin or ofloxacin would be acceptable if adequacy for treating pneumococcal infections were likely. New macrolides, such as azithromycin and clarithromycin, and new fluoroquinolones, such as temafloxacin and sparfloxacin, have theoretical advantages over previously available drugs, but superior efficacy has not yet been demonstrated satisfactorily. Pneumococcal resistance in various parts of the world is modifying traditional treatment. Currently, there is no drug of choice for the empirical treatment of community-acquired pneumonia.

Adult↗
PubMed1983

Mycobacterium chelonei: report of a case of septicemia and review of the literature.

Mycobacterium chelonei was originally included in group IV of Runyon's classification of "anonymous" or "atypical" mycobacteria. Although a frequent contaminant without clinical significance, this organism has definite pathogenic potential. A compromised host with M. chelonei septicemia and disseminated candidiasis is described. A review of the literature on M. chelonei human infections is also presented.

Alcoholism↗
PubMed2001

Dry eye disease: the scale of the problem.

Dry eye disease, or keratoconjunctivitis sicca, is among the most frequently established diagnoses in ophthalmology; in Germany, one in four patients consulting an ophthalmologist complains of the symptoms of dry eye. Although epidemiological studies investigating the prevalence of dry eye disease are rare, published studies indicate that up to 20% of adults aged 45 years or more experience dry eye symptoms. Recent studies have shown that immunologic changes play a role in the pathogenesis of dry eye, not only in Sjögren's syndrome, but also in postinfectious and age-related conditions. Despite increasing understanding of the pathogenic factors involved in dry eye disease, there has been a lack of consensus on diagnostic criteria, classification of disease states, and the aims and interpretation of specific diagnostic tests. There is a need, therefore, for standardization of disease terminology and diagnostic tests in order to improve the usefulness of epidemiological and clinical investigation of this important ocular disorder.

Dry Eye Syndromes↗
PubMedDate not supplied

[Periodic activity in the EEG].

Definition of Periodic EEG Activities. Proposal of a classification. Clinical conditions with periodic EGG activities. Discussion of the relations between myoclonic and epileptic symptoms. Proposed pathogenic hypothesis (biochemistry, immunology). Bibliogarphy.

Adult↗
PubMed1977

A method of diagnosing intramammary infection in dairy cows for large experiments.

Diagnosis of microbial infections in the udders of cows in commercial dairy farms for large experiments cannot be without error. Limitations of sampling method and routine prevent collection of the necessary information for sure diagnosis. However, with an organized method of repeated bacteriological examinations using consistent and proven methods of aseptic sampling the errors were shown to be very low. A method based on bacteriological tests on aseptic milk samples was used in 32 herds (approximately 2000 cows) for a 3-year period. This is described and examined in terms of other criteria to validate its use in experimental work. With this method it was not difficult to differentiate between those quarters which regularly shed pathogens and those which did not. Other evidence indicated that it was reasonable to assume that this classification accurately distinguished between infected and uninfected quarters. The errors using this method were quite small: when measuring the state of infection of all quarters in the herds the errors did not exceed 1%. Some small modifications to the method described are suggested to improve further its diagnostic accuracy.

Animals↗
PubMedDate not supplied

Helicases as antiviral drug targets.

Helicases catalytically unwind duplex DNA or RNA using energy derived from the hydrolysis of nucleoside triphosphates and are attractive drug targets because they are required for viral replication. This review discusses methods for helicase identification, classification and analysis, and presents an overview of helicases that are necessary for the replication of human pathogenic viruses. Newly developed methods to analyze helicases, coupled with recently determined atomic structures, have led to a better understanding of their mechanisms of action. The majority of this research has concentrated on enzymes encoded by the herpes simplex virus (HSV) and the hepatitis C virus (HCV). Helicase inhibitors that target the HSV helicase-primase complex comprised of the UL5, UL8 and UL52 proteins have recently been shown to effectively control HSV infection in animal models. In addition, several groups have reported structures of the HCV NS3 helicase at atomic resolutions, and mechanistic studies have uncovered characteristics that distinguish the HCV helicase from related cellular proteins. These new developments should eventually lead to new antiviral medications.

Animals↗
PubMed1980

Yersinia enterocolitica infections and rheumatic diseases.

Yersinia enterocolitica (Y. ent.) infections are rather frequently complicated by acute reactive inflammation in the connective tissue, especially in the joints. At this stage of the disease the specific diagnosis can be obtained either by bacterial isolation and identification from the feces and/or mesenterial lymph nodes, or by serological methods. Serodiagnostics are frequently the only method during the complication phase, since the bacteria have often disappeared from the feces by this stage of the disease. Specific Y. ent. serodiagnostics are benefitted by the fact that no antisera cross-react with the serotype 3 thermostable O-antigen. A titre of greater than or equal to 80 is therefore highly indicative of a recent or current Y. ent. infection. In the absence of other known arthritogenic agents the Y. ent. antibodies are highly indicative of the Y. ent. etiology of a current disease. The Y. ent. complications affect most inflammatory reactive diseases, acute as well as chronic. In an area in which Y. ent. infections are endemic, Y. ent. is the most frequent cause of acute and chronic arthritis. The present results indicate that not all cases of acute Y. ent. arthritis remit, but some persist, usually with an intermittent course, and develop into rheumatoid arthritis or allied conditions. This suggests a common pathogenic mechanism in most inflammatory rheumatic diseases. It is proposed that the time has come for a classification of these diseases based on their etiology, in order to replace the present symptom-based treatment with a causal one, and to institute prophylactic measures. The pathology is not exclusive to Y. ent., but can presumably also be brought about by other bacteria, such as gonococci, meningococci, salmonellae, shigellae, and brucellae, possibly by their content of lipopolysaccharide.

Adult↗
PubMed2026

Deciphering the Role of LNX2 as a Potential Contributor to Neurodevelopmental Disorders.

BACKGROUND/OBJECTIVES: Attention-deficit/hyperactivity disorder (ADHD) is a common neurodevelopmental condition characterized by a complex and multifactorial genetic architecture. In this study, we report a male patient, born to non-consanguineous healthy parents, presenting with ADHD and oppositional defiant disorder (ODD). METHODS: Trio-based whole-exome sequencing (WES) was performed in the proband and both parents. Variant classification was performed according to American College of Medical Genetics and Genomics (ACMG) guidelines, and the potential pathogenicity of the identified variant was further assessed through multiple in silico prediction algorithms and protein structural analyses. RESULTS: WES identified a homozygous variant in the LNX2 gene (NM_153371.4: c.1165G>A, p.Ala389Thr), classified as a variant of uncertain significance (VUS) and supported by multiple in silico predictions. LNX2 is expressed during brain development and encodes an E3 ubiquitin ligase involved in neuronal differentiation and synaptic function. The identified variant is located within the PDZ2 domain, a functionally relevant region involved in protein-protein interactions. Although the variant is reported in population databases (gnomAD ID: rs148429804), it has not been associated with any clinical phenotype, and its presence in the homozygous state has been reported only once, remaining extremely rare and lacking clinical annotation. Structural modelling predicted localized rearrangement of the hydrogen-bonding network within the PDZ2 domain without major conformational changes. Integrative transcriptomic, and single-cell analyses further supported the biological relevance of LNX2 in neurodevelopment, highlighting its preferential association with neuronal projection-cell networks, synaptic vesicle trafficking pathways, and neuron-specific regulatory programs. CONCLUSION: Although the identified LNX2 variant cannot be considered causative for the patient's phenotype and a definitive disease-gene relationship cannot be established based on a single individual, the complementary genetic, structural, and transcriptomic findings support the biological plausibility of LNX2 as a candidate gene for neurodevelopmental disorders. Additional independent patients and functional studies will be required to clarify its contribution to human disease.

Child↗
PubMed1976

Electrophoresis of water-insoluble leptospiral proteins and the taxonomy of Leptospira.

The cellular debris obtained after lysis of Leptospira were solubilized by sodium dodecyl sulfate and studied by polyacrylamide electrophoresis, comparing the molecular weight and relative concentration of the protein bands. The percentage similarity of 30 characteristic bands from 21 Leptospira strains, belonging to the main pathogenic and some saprophytic serogroups, was calculated; A partial matching of the derived dendrogram with the other classifications was observed.

Bacterial Proteins↗
PubMed2002

[In search of a typology of drugs used by the aged living in the community].

BACKGROUND: The objective was to identify the determining factors of drug utilization in the elderly by using several classes of drugs. METHODS: We studied drug utilization in 1373 women, aged 75 and over, selected in the EPIDOS study concerning hip fractures in the Languedoc-Roussillon region (France) and in 2525 men and women, aged 60 and over selected for the POLA study (age-related eye diseases) in the town of Sete. We defined a priori three types of drugs considering the perceived risk by the physician, and the potential demand from the patient (I - Etio-pathogenic drugs with iatrogenic risk; II - Symptomatic drugs without perceived risk; III - Psychotropic drugs). To validate this classification, we studied drug consumption by age and sex, its relationship with mortality and education level, the fact of living alone, subjective health and level of physical activity. RESULTS: The total drug consumption level, measured by the number of drugs taken daily, was higher in women but, at any age, men more often used type I drugs. Survival was also related to the number of type I drugs, that may be considered as an indicator of serious pathology. Inversely, no relationship was observed between survival and the number of type II drugs that grouped symptomatic or comfort drugs. CONCLUSION: Such typology can help to understand drug over-utilization in the elderly.

Aged↗
PubMedDate not supplied

Thoughts about the classification of small vessel vasculitis.

The classification of small vessel vasculitis has markedly changed over the past 150 years. With the discovery of anti-neutrophil cytoplasmic autoantibodies (ANCA), renewed interest in the field has spawned investigations into the immunopathogenesis of small vessel vasculitis. This review will describe the historical basis of the classification of vasculitis, the current nosology based on the Chapel Hill Nomenclature for vasculitis and then both pathogenic and clinical reasons that support the use of the term "ANCA small vessel vasculitis" to describe the pauci-immune necrotizing small vessel vasculitides, including microscopic polyangiitis, Wegener's granulomatosis, the Churg-Strauss syndrome and pauci-immune necrotizing and crescentic glomerulonephritis.

Antibodies, Antineutrophil Cytoplasmic↗
PubMed1975

Arenavirus taxonomy: a review.

Despite a late beginning, the construction of the arenavirus taxon and its placement in the scheme of the International Committee on Taxonomy of Viruses has now been completed. The bringing together of the member viruses has already provided valuable indications of promising laboratory and field study approaches; in the future this classification will contribute further to our understanding of the natural history and disease processes of the human pathogens of the group.

Arboviruses↗
PubMed1979

Comparative study of the growth inhibition of vegetative hyphae of some dermatophytes and keratinophilic fungi on glucose-peptone-agar with griseofulvin.

By the method of Jesenská and Danilla [29] the growth inhibition of vegetative hyphae of colonies of 12 species of dermatophytes and keratinophilic fungi on the surface of glucose-peptone-agar with incorporated griseofulvin has been established. It could be concluded that the sensitivity of hyphae to griseofulvin is neither connected with the taxonomic position and ecological classification of these microscopic fungi, nor with their ability to survive in the soil, or with their pathogenity etc. - In Trichophyton rubrum some hyphae were found which were extremely resistant to griseofulvin. - The extablished values allow to make an objective comparison of the sensitivity level of vegetative hyphae of dermatophytes and keratinophilic fungi to griseofulvin in further geographical regions, at different times and in different laboratories. - In the discussion further possibilities in this direction are developed.

Arthrodermataceae↗
PubMed2003

Dominant spinocerebellar ataxias: a molecular approach to classification, diagnosis, pathogenesis and the future.

The capacity to use molecular techniques to establish the genetic diagnoses of the autosomal dominant ataxias has revolutionized the field. It is now possible to systematically classify these disorders according to the nature of the causative mutation, with implications for diagnostic testing, analysis of pathogenesis and therapeutic strategies. Here, the disorders are grouped into ataxias caused by CAG repeat expansions that encode polyglutamine, ataxias caused by mutations in ion channels, ataxias caused by repeat expansions that do not encode polyglutamine, and ataxias caused by point mutations. The clinical, pathological, genetic and pathogenic features of each disorder are considered and the current status and future of diagnosis and therapy are reviewed in light of this classification scheme.

Humans↗