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PubMedDate not supplied

Changes in lysosomal hydrolases from cord blood of vigorous and asphyxiated Nigerian newborn infants.

The activities of lysosomal hydrolases, beta-glucuronidase and acid phosphatase, were assayed in cord sera from forty-seven non asphyxiated babies and eleven babies who were severely asphyxiated at birth. Significant changes of these enzymes were observed in cord serum. Compared to the control group, serum beta glucuronidase (determined at pH 4.5 using fluorogenic substrate, 4-methyl-umbelliferyl-B-D glucuronide) was 2.2 fold higher (P less than 0.01) in the asphyxiated babies. Similarly, serum acid phosphatase activity estimated by using 4-methyl-umbelliferyl-phosphate was 1.8 fold higher (P less than 0.01) in asphyxia neonatorum. The clinical relevance of this finding lies in its ultimate application, using the lysosomal hydrolase activity in scalp blood sample to evaluate objectively the foetal well-being.

Acid Phosphatase↗
PubMed1980

Effect of acute vigorous exercise on lipoprotein lipase activity of adipose tissue and skeletal muscle in physically active men.

Ten well-trained men ran a distance of 20 km in the morning after overnight fasting. Lipoprotein lipase (LPL) activity was determined from heparin eluates of adipose tissue and skeletal muscle before and after the exercise. The mean LPL activity rose 2.1-fold in skeletal muscle (p < 0.01) and by 20 % in adipose tissue (p < 0.05) during the running. No significant change occurred in serum lipid or lipoprotein concentrations. The plasma insulin decreased and plasma glucagon increased during the exercise. The muscle LPL increment was significantly related to the fall of insulin/glucagon ratio. The results show that during exercise the skeletal muscle is adapted for increased uptake of circulating triglycerides which are either utilized immediately or used for restoration of muscle lipid stores after the end of exercise.

Adipose Tissue↗
PubMedDate not supplied

[Validation of Guttman scales of vigor and readiness to make social contacts in the aged].

Two Guttman-scales for the assessment of functional health and sociability of aged persons were examined in several different samples concerning their scalability, dimensionality, and validity. They proved well suited for a relatively broad use. Contrary to other measures of ADL-functioning, our functional health scale discriminates the upper levels of functional competence. In regard to expert-ratings as well as to measures of actual behavior, both scales proved to be valid. The functional health scale correlates significantly with the Barthel Index but shows a better sensibility for changes of functional competence during a half-year period.

Activities of Daily Living↗