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Acetylcholinesterase activity of the mesencephalic trigeminal nucleus of Triturus cristatus carnifex.

This study deals with acetylcholinesterase activity in mesencephalic trigeminal nucleus neurons of the newt. These cells appear clearly positive to histochemical reaction. Electron microscope investigations revealed the fine localization of the enzyme in cell bodies. On the basis of the ultrastructural pattern of AChE distribution some considerations on the possible sites of utilization are discussed.

Acetylcholinesterase↗

Sarcomagenesis and myofibril maturation during limb regeneration and development of the newt, Triturus alpestris and salamander, Salamandra salamandra.

During limb regeneration of the salamander, Salamandra salamandra, muscles dedifferentiate and then mesenchymal cells appear, which subsequently, differentiate into muscle. Both in regenerates and in developing limbs of newts larvae mesenchymal cells begin to differentiate into myoblasts, which contain both thin (7 nm) and thick (14 nm) filaments, which are often associated with microtubules, developing a cytoskeletal network, which contribute directly to the shape of myoblasts and a linear alignment of the myofibrils. Both thin and thick filaments appear as a tandem near the periphery of the myoblasts, where myofibrilogenesis occurs. In the early stage of sarcomagenesis the appearance of "Z-bodies" was observed. They were intimately associated with the tandem of filaments thus forming the primary sarcomere units. Consequently the "Z-bodies" coalesce to form primitive Z-lines. The maturation of the primary sarcomeres occurs during fibrilogenesis and is manifested by the gradual thining of the Z-line and thickening of the M-line.

Animals↗